pAAVLINKv1-pCALM1-Cre-3'-CHD2-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-3'-CHD2-FLAG               6645 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6645)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 9, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3358
                     /codon_start=1
                     /gene="CHD2"
                     /gene_synonym="DEE94; EEOC"
                     /product="chromodomain-helicase-DNA-binding protein 
                     2isoform 1"
                     /EC_number="3.6.4.12"
                     /label=CHD2
                     /note="isoform 1 is encoded by transcript variant 1;CHD-2; 
                     ATP-dependent helicase CHD2"
                     /db_xref="GI:118421089"
                     /db_xref="CCDS:CCDS10374.2"
                     /db_xref="GeneID:1106"
                     /db_xref="HGNC:HGNC:1917"
                     /db_xref="MIM:602119"
                     /protein_id="NP_001262.3"
                     /translation="KRRGPTIKISGVQVNVKSIIQHEEEFEMLHKSIPVDPEEKKKYCL
                     TCRVKAAHFDVEWGVEDDSRLLLGIYEHGYGNWELIKTDPELKLTDKILPVETDKKPQG
                     KQLQTRADYLLKLLRKGLEKKGAVTGGEEAKLKKRKPRVKKENKVPRLKEEHGIELSSP
                     RHSDNPSEEGEVKDDGLEKSPMKKKQKKKENKENKEKQMSSRKDKEGDKERKKSKDKKE
                     KPKSGDAKSSSKSKRSQGPVHITAGSEPVPIGEDEDDDLDQETFSICKERMRPVKKALK
                     QLDKPDKGLNVQEQLEHTRNCLLKIGDRIAECLKAYSDQEHIKLWRRNLWIFVSKFTEF
                     DARKLHKLYKMAHKKRSQEEEEQKKKDDVTGGKKPFRPEASGSSRDSLISQSHTSHNLH
                     PQKPHLPASHGPQMHGHPRDNYNHPNKRHFSNADRGDWQRERKFNYGGGNNNPPWGSDR
                     HHQYEQHWYKDHHYGDRRHMDAHRSGSYRPNNMSRKRPYDQYSSDRDHRGHRDYYDRHH
                     HDSKRRRSDEFRPQNYHQQDFRRMSDHRPAMGYHGQGPSDHYRSFHTDKLGEYKQPLPP
                     LHPAVSDPRSPPSQKSPHDSKSPLDHRSPLERSLEQKNNPDYNWNVRKT"
     misc_feature    2462..2467
                     /label=SDS
     CDS             3365..3430
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3434..3865
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3578..3583
                     /label=SDS
     repeat_region   3908..4048
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3908..4037
                     /label=AAV2 ITR
     repeat_region   3909..4049
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4123..4578
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4860..4964
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4965..5825
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5996..6584
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca gggaaaagga gaggtccaac
     1501 aatcaagata tccggagttc aggttaatgt gaaatccatt atccaacatg aagaggagtt
     1561 tgagatgctg cataaatcta tccctgtgga ccctgaagaa aaaaaaaaat actgcttaac
     1621 ctgtcgtgtc aaagctgcac attttgatgt agagtggggg gtggaagatg attctcgcct
     1681 gttgctgggg atttatgaac atggctatgg aaactgggag ttaattaaaa cagacccaga
     1741 gcttaaatta actgacaaaa ttctgccggt ggagacagat aaaaagcctc aggggaagca
     1801 gctacagacc cgagcggatt acttgttgaa gctgctcaga aagggtctgg agaagaaggg
     1861 ggctgtgaca ggtggggaag aggccaaatt aaagaagcgg aagcctcggg taaagaagga
     1921 aaacaaagtg cccaggctga aagaggagca tggaattgag ctttcatctc ctaggcattc
     1981 agataatcca tcagaagagg gagaagtgaa agatgatggc ttggaaaaaa gtccaatgaa
     2041 aaaaaaacag aagaagaaag agaacaagga gaacaaggag aaacaaatga gttctaggaa
     2101 agacaaagaa ggggacaagg aaagaaagaa gtcaaaagat aagaaagaga agcctaaaag
     2161 tggtgatgcc aaatcttcga gtaaatcaaa gcgatctcag ggtcctgtcc atattacagc
     2221 aggaagtgaa cctgtcccca ttggagagga tgaggatgat gatctggacc aggagacatt
     2281 cagcatatgt aaggagagga tgaggcccgt gaaaaaggca ctgaaacagc tcgacaaacc
     2341 tgacaagggg ctcaacgtgc aagaacagct ggaacacacc cggaactgcc tgctgaaaat
     2401 cggagaccgg atagccgagt gccttaaagc ctactcagat caggagcaca tcaaactctg
     2461 gaggaggaac ctatggattt ttgtttccaa gtttacagaa tttgatgctc gaaaactgca
     2521 taagttatac aagatggctc ataagaaaag gtctcaagaa gaagaggagc aaaagaagaa
     2581 agacgacgtg actgggggta agaaaccatt tcgtccagag gcctcaggct ccagccggga
     2641 ctctctgata tctcagtccc atacctcaca caaccttcac cctcagaagc ctcatttgcc
     2701 tgcctcccat ggcccacaga tgcatggaca cccaagagat aactacaatc accccaacaa
     2761 gagacacttc agtaatgcag atcgaggaga ctggcagagg gaaagaaagt tcaactatgg
     2821 tggtggcaac aacaatccac catggggaag cgacaggcac catcagtatg agcagcactg
     2881 gtacaaggac caccattatg gggaccggcg acatatggat gcccaccgtt ccggaagcta
     2941 tcgacccaac aacatgtcca gaaagaggcc ttatgaccag tacagcagtg accgagacca
     3001 ccggggacac agagattatt atgacaggca ccatcatgac tccaagcgga ggagatccga
     3061 tgaatttagg cctcaaaatt accaccagca ggatttccga cgaatgtctg atcaccgccc
     3121 cgctatgggc taccatggcc agggaccctc agaccattac cgctctttcc acacagataa
     3181 actgggggaa tataaacagc ctctaccccc attgcaccct gcagtctcag atcctcgctc
     3241 acccccttct cagaaatctc ctcacgattc caagtcaccc ctggatcata ggtctccttt
     3301 ggagagatca ctagaacaga aaaacaaccc agattataac tggaatgttc ggaaaacatg
     3361 tacagactac aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga
     3421 cgatgacaag taactcgaga taatcaacct ctggattaca aaatttgtga aagattgact
     3481 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     3541 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggtta
     3601 gttcttgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     3661 ctgttgggca ctgacaattc cgtggtgttt atttgtgaaa tttgtgatgc tattgcttta
     3721 tttgtaacca tctagcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     3781 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     3841 gggggagatg tgggaggttt tttaaagatc tggagaccgg tgtggcacgt gcggaccgag
     3901 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     3961 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     4021 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
     4081 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     4141 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct
     4201 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     4261 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     4321 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4381 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4441 gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     4501 cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     4561 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     4621 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     4681 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     4741 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     4801 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     4861 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     4921 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     4981 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5041 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5101 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5161 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5221 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5281 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5341 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5401 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5461 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     5521 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     5581 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     5641 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     5701 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     5761 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     5821 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     5881 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5941 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6001 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6061 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6121 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     6181 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6241 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6301 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6361 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6421 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6481 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6541 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6601 cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//