| 质粒名称: | pAAV-CAG-DIO-H2B-mBaoJin-WPRE-polyA |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000668 |
| 产品用途: | AAV, expresses H2B-mBaoJin in cells |
| 存储实验室: | Hu Zhao Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl |
|
插入基因信息 |
|
| 插入基因名称: | H2B-mBaoJin |
| 物种来源: | H.sapiens, Obelia Medusa |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Bright and stable monomeric green fluorescent protein derived from StayGold
Zhang H., Leslov G.D., Subach O.M., Zhang W., Kuzmicheva T.P., Vlaskina A.V., Samygina V.R., Chen L., Ye X., Nikolaeva A.Y., Gabdulkhakov A., Papadaki S., Qin W., Borshchevskiy V., Perfilov M.M., Gavrikov A.S., Drobizhev M., Mishin A.S., Piatkevich K.D.
Nat. Methods 2024 214 21, 657–665 (2024)
|
买家导航
pAAV-CAG-DIO-H2B-mBaoJin-WPRE-polyA载体质粒基本信息
pAAV-CAG-DIO-H2B-mBaoJin-WPRE-polyA质粒图谱载体图谱和pAAV-CAG-DIO-H2B-mBaoJin-WPRE-polyA载体序列质粒序列多克隆位点信息
pAAV-CAG-DIO-H2B-mBaoJin-WPRE-polyA质粒载体简介
pAAV-CAG-DIO-H2B-mBaoJin-WPRE-polyA质粒序列载体序列
LOCUS pAAV-CAG-DIO-H2B-mBaoJin-WPRE-polyA 6111 bp DNA circular SYN 16-JAN-2025
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6111)
AUTHORS Rui Lin
TITLE Direct Submission
JOURNAL Exported Apr 30, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6111
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature complement(3..11)
/label=glycine-serine-glycine linker
rep_origin complement(126..793)
/direction=LEFT
/label=pUC ori
CDS complement(941..1801)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(1802..1906)
/gene="bla"
/label=AmpR promoter
rep_origin complement(1932..2387)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication;
arrowindicates direction of (+) strand synthesis"
misc_feature 2458..2647
/label=ITR Addgene
misc_feature 2648..2653
/label=5' splice site
misc_feature 2651..3587
/label=CAG
enhancer 2774..2899
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2901..3178
/label=chicken beta-actin promoter
primer_bind 3579..3600
/label=0711-PM960-F
misc_feature 3624..3657
/label=lox2722
primer_bind 3639..3659
/label=Clone118-R
misc_feature 3708..3741
/label=loxP
primer_bind 3724..3752
/label=Clone118-F
CDS complement(3753..4454)
/codon_start=1
/label=mBaoJin
/translation="MVSKGEEENMASTPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKG
KYVCTSGKLPMSWAALGTTFGYGMKYYTKYPSGLKNWFREVMPGGFTYDRHIQYKGDGS
IHAKHQHFMKNGTYHNIVEFTGQDFKENSPVLTGDMNVSLPNEVPQIPRDDGVECPVTL
LYPLLSDKSKYVEAHQYTICKPLHNQPAPDVPYHWIRKQYTQSKDDAEERDHICQSETL
EAHLKGMDELYK"
primer_bind 3753..3774
/label=0711-PM960-R
primer_bind 3780..3804
/label=0714-StayBlue-R
primer_bind complement(4394..4428)
/label=0714-StayBlue-F
primer_bind complement(4433..4454)
/label=0711-PM960-F
misc_feature 4455..4857
/label=MCS
/note="multiple cloning site"
CDS complement(4470..4847)
/codon_start=1
/label=H2B
/translation="MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKV
LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
GELAKHAVSEGTKAVTKYTSSK"
primer_bind complement(4856..4889)
/label=Clone118-R
misc_feature 4871..4904
/label=lox2722
misc_feature 4955..4988
/label=loxP
primer_bind complement(4955..4972)
/label=Clone118-F
misc_feature 5013..5601
/label=WPRE
/note="woodchuck hepatitis virus
posttranscriptionalregulatory element"
CDS complement(5484..5495)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 5644..5765
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature 5868..6057
/label=ITR Addgene
ORIGIN
1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
2641 gaagatcgta ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
2701 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
2761 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
2821 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
2881 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc
2941 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
3001 gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg
3061 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
3121 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
3181 agtcgctgcg acgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
3241 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
3301 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
3361 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg
3421 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
3481 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
3541 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgga tccggtaccg
3601 gatcctctag agtcgactcc ggaataactt cgtataggat actttatacg aagttatgca
3661 gaatggtagc tggattgtag ctgctattag caatatgaaa cctcttaata acttcgtata
3721 gcatacatta tacgaagtta tggcgcgcct tacttataca gctcgtccat gccctttaag
3781 tgtgcttcga gagtctctga ttgacagata tgatcgcgtt cctcggcatc atctttgctt
3841 tgtgtgtatt gtttacgaat ccagtgatat gggacatcag gtgctggttg attatgaaga
3901 ggcttgcaga ttgtatattg gtgagcctcg acgtattttg atttatccga taataaagga
3961 taaagcaagg tcactgggca ttctactcca tcatctctgg gtatttgtgg gacttcattc
4021 ggtaatgaga cattcatatc tccagttaag actggactat tttctttaaa atcctgaccg
4081 gtaaattcta caatgttgtg ataagtccca tttttcataa agtgttggtg ttttgcatgg
4141 atactcccat cgcctttata ttgaatatga cgatcgtagg taaagcctcc gggcattact
4201 tcacgaaacc agttcttcag tccactagga tatttggtat aatatttcat tccataacca
4261 aaggttgtcc caagtgctgc ccatgacatc ggtagttttc cacttgtaca aacatatttt
4321 cctttatgag aaccttcatg tgagttccct tcaccttcgc cttcaacggt aaacgatttg
4381 ccattgatgg ttcctttaag ttgaaattta aatggtgtac tagccatgtt ttcctcctct
4441 ccctttgaga ccataagctt gagctcgagc ttggagctgg tgtacttggt gaccgccttg
4501 gtgccctcgg acacggcgtg cttggccagc tcgccgggca gcagcaggcg cacggccgtc
4561 tggatctcgc gggatgtgat ggtggagcgc ttgttgtagt gcgccaggcg ggaagcctct
4621 cccgcgatgc gctcgaagat gtcgttgacg aaggagttca tgatgcccat ggccttggac
4681 gagatgccgg tgtcggggtg gacctgcttc agcaccttgt acacgtagat ggagtagctc
4741 tctttgcggc tgcgcttgcg cttcttgccg tctttcttct gggctttggt gacggctttc
4801 ttggagccct ttttaggggc cggagcggat tttgccggtt caggcatggt ggctagcgac
4861 cggtgctagc ataacttcgt ataaagtatc ctatacgaag ttatttgcct taacccagaa
4921 attatcactg ttattcttta gaatggtgca aagaataact tcgtataatg tatgctatac
4981 gaagttatga attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
5041 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
5101 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
5161 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
5221 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
5281 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
5341 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
5401 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
5461 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
5521 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
5581 ccctttgggc cgcctccccg catcgatacc gtcgacccgg gcggccgctt cgagcagaca
5641 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
5701 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
5761 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg
5821 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat cttcctagag
5881 catggctacg tagataagta gcatggcggg ttaatcatta actacaagga acccctagtg
5941 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
6001 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc
6061 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc t
//
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