pAC1430

LOCUS       pAC1430               15497 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  AMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker,
            and USER cassette (PacI/Nt.BbvCI).
ACCESSION   .
VERSION     .
KEYWORDS    pAC1430
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15497)
  AUTHORS   Vanegas KG, Jarczynska ZD, Strucko T, Mortensen UH
  TITLE     Cpf1 enables fast and efficient genome editing in Aspergilli.
  JOURNAL   Fungal Biol Biotechnol. 2019 May 1;6:6. doi: 
            10.1186/s40694-019-0069-6. eCollection 2019.
  PUBMED    31061713
REFERENCE   2  (bases 1 to 15497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15497
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      509..5759
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
     protein_bind    complement(6072..6105)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(6312..6331)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(6337..6356)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        6398..6489
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6490..7350
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6708..6727)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7472..8060
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7961..7980
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8214..8231
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     8329..8346
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8330..8346
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8816..8849
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(10979..10999)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(11003..11083)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(11090..11137)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(11138..14821)
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
ORIGIN
        1 catacgtagc aagatagacc gcaagaaaat gatgctcgga atccatattg actcacgaaa
       61 atttctttct ttttgccccc ctcatctgtt gcctgggagg tgagtcaaat tgcccctcac
      121 taaacgctcc tttgcttttc ccgaacttgg cccgttcgag agcatgatca gcacaaaggc
      181 ttaacttggg cggtgattct gctgtctcgg ctgaggtctt aattaaaccc tcagcattaa
      241 accctcagcg gaaacagcta tgaccatgag atcttcccgg gcccagtata tgttccgcag
      301 atgactggag ctctgccata cgtgccctct caagcaccat ttgttccatc tacagagact
      361 agtcaccaac tagtctatca agactcacag ggtacattgc tgagaccaac tgaccagagg
      421 cagggtagcg gattgacggc tccatctcct tcacttacaa ggtctattga aagcccttta
      481 gcatcaccaa gcggagaata gattgttaag cttatttttt gtatactgtt ttgtgatagc
      541 acgaagtttt tccacggtat cttgttaaaa atatatattt gtggcgggct tacctacatc
      601 aaattaataa gagactaatt ataaactaaa cacacaagca agctacttta gggtaaaagt
      661 ttataaatgc ttttgacgta taaacgttgc ttgtatttat tattacaatt aaaggtggat
      721 agaaaaccta gagactagtt agaaactaat ctcaggtttg cgttaaacta aatcagagcc
      781 cgagaggtta acagaaccta gaaggggact agatatccgg gtagggaaac naaaaaaaaa
      841 aaacaagaca gccacatatt agggagacta gttagaagct agttccagga ctaggaaaat
      901 aaaagacaat gataccacag tctagttgac aactagatag attctagatt gaggccaaag
      961 tctctgagat ccaggttagt tgcaactaat actagttagt atctagtctc ctataactct
     1021 gaagctagaa taacttacta ctattatcct caccactgtt cagctgcgca aacggagtga
     1081 ttgcaaggtg ttcagagact agttattgac tagtcagtga ctagcaataa ctaacaaggt
     1141 attaacctac catgtctgcc atcaccctgc acttcctcgg gctcagcagc cttttcctcc
     1201 tcattttcat gctcattttc cttgtttaag actgtgacta gtcaaagact agtccagaac
     1261 cacaaaggag aaatgtctta ccactttctt cattgcttgt ctcttttgca ttatccatgt
     1321 ctgcaactag ttagagtcta gttagtgact agtccgacga ggacttgctt gtctccggat
     1381 tgttggagga actctccagg gcctcaagat ccacaacaga gccttctaga agactggtca
     1441 ataactagtt ggtctttgtc tgagtctgac ttacgaggtt gcatactcgc tccctttgcc
     1501 tcgtcaatcg atgagaaaaa gcgccaaaac tcgcaatatg gctttgaacc acacggtgct
     1561 gagactagtt agaatctagt cccaaactag cttggatagc ttacctttgc cctttgcgtt
     1621 gcgacaggtc ttgcagggta tggttccttt ctcaccagct gatttagctg ccttgctacm
     1681 ctcacggcgg atctgcataa agagtggcta gaggttataa attagcactg atcctaggta
     1741 cggggctgaa tgtaacttgc cyttcctttc tcatcgcgcg gcaagacagg cttgctcaaa
     1801 ttcctaccag tcacaggggt atgcacggcg tacggaccac ttgaactagt cacagattag
     1861 ttagcaacta gtctgcattg aatggctgta cttacgggcc ctcgccattg tcctgatcat
     1921 ttccagcttc accctcgttg ctgcaaagta gttagtgact agtcaaggac tagttgaaat
     1981 gggagaagaa actcacgaat tctcgacacc cttagtattg tggtccttgg acttggtgct
     2041 gctatatatt agctaataca ctagttagac tcacagaaac ttacgcagct cgcttgcgct
     2101 tcttggtagg agtcggggtt gggagaacag tgccttcaaa caagccttca taccatgcta
     2161 cttgactagt cagggactag tcaccaagta atctagatag gacttkcctt tggcctccat
     2221 cagttccttc atagtgggag gtccattgtg caatgtaaac tccatgccgt gggagttctt
     2281 gtccttcaag tgcttgacca atatgtttct gttggcagag ggaacctgtc aactagttaa
     2341 taactagtca gaaactagta tagcagtaga ctcactgtac gcttgaggca tcccttcact
     2401 cggcagtaga cttcatatgg atggatatca ggcacgccat tgtcgtcctg tggactagtc
     2461 agtaactagg cttaaagcta gtcgggtcgg cttactatct tgaaatccgg cagcgtaagc
     2521 tccccgtcct taactgcctc gagatagtga cagtactctg gggactttcg gagatcgtta
     2581 tcgcgaatgc tcggcatact aatcgttgac tagtcttgga ctagtcccga gcaaaaagga
     2641 ttggaggagg aggaggaagg tgagagtgag acaaagagcg aaataagagc ttcaaaggct
     2701 atctctaagc agtatgaagg ttaagtatct agttcttgac tagatttaaa agagatttcg
     2761 actagttatg tacctggagt ttggatatag gaatgtgttg tggtaacgaa atgtaagggg
     2821 gaggaaagaa aaagtcggtc aagaggtaac tctaagtcgg ccattccttt ttgggaggcg
     2881 ctaaccataa acggcatggt cgacttagag ttagctcagg gaatttaggg agttatctgc
     2941 gaccactgga ctctcaccgt ttccattggg gtactaacat agccatcaaa tgccggcccg
     3001 tgtttaatct cctcgagatg gctgggctca catgcgttct actcaaaaac tcttatacct
     3061 aggccctctc ccaatcgtgt atgtttggac cttcctctct cacactttgg ataacatcaa
     3121 gcatactgca gattttgacc tagttaacaa ggcacatcat gctatcactt ccaacactct
     3181 tgctgagcgc gttctgcctt gcctttctgg ttctacgaag tatccagtcc cgcctgcaac
     3241 gccgcagaaa cgccaaaagg tggggttgtc aaccgccagc tagagctcca tcgggattct
     3301 ttggcattca gccgttcctc ggtggtcgca gataactccc taaattccct gagctaactc
     3361 taagtcgacc atgccgttta tggttagcgc ctcccaaaaa ggaatggccg acttagagtt
     3421 acctcttgac cgactttttc tttcctcccc cttacatttc gttaccacaa cacattccta
     3481 tatccaaact ccaggtacat aactagtcga aatctctttt aaatctagtc aagaactaga
     3541 tacttaacct tcatactgct tagagatagc ctttgaagct cttatttcgc tctttgtctc
     3601 actctcacct tcctcctcct cctccaatcc tttttgctcg ggactagtcc aagactagtc
     3661 aacgattagt atgccgagca ttcgcgataa cgatctccga aagtccccag agtactgtca
     3721 ctatctcgag gcagttaagg acggggagct tacgctgccg gatttcaaga tagtaagccg
     3781 acccgactag ctttaagcct agttactgac tagtccacag gacgacaatg gcgtgcctga
     3841 tatccatcma tatgaagtct actgccgagt gaagggatgc ctcaagcgta cagtgagtct
     3901 actgctatac tagtttctga ctagttatta actagttgac aggttccctc tgccaacaga
     3961 aacatattgg tcaagcactt gaaggacaag aactcccacg gcatggagtt tacattgcac
     4021 aatggacctc ccactatgaa ggaactgatg gaggccaaag gmaagtccta tctagattac
     4081 ttggtgacta gtccctgact agtcaagtag catggtatga aggcttgttt gaaggcactg
     4141 ttctcccaac cccgactcct accaagaagc gcaagcgagc tgcgtaagtt tctgtgagtc
     4201 taactagtgt attagctaat atatagcagc accaagtcca aggaccacaa tactaagggt
     4261 gtcgagaatt cgtgagtttc ttctcccatt tcaactagtc cttgactagt cactaactac
     4321 tttgcagcaa cgagggtgaa gctggaaatg atcaggacaa tggcgagggc ccgtaagtac
     4381 agccattcaa tgcagactag ttgctaacta atctgtgact agttcaagtg gtccgtacgc
     4441 cgtgcatacc cctgtgactg gtaggaattt gagcaagcct gtcttgccgc gcgatgagaa
     4501 aggaarggca agttacattc agccccgtac ctaggatcag tgctaattta taacctctag
     4561 ccactcttta tgcagatccg ccgtgagkgt agcaaggcag ctaaatcagc tggtgagaaa
     4621 ggaaccatac cctgcaagac ctgtcgcaac gcaaagggca aaggtaagct atccaagcta
     4681 gtttgggact agattctaac tagtctcagc accgtgtggt tcaaagccat attgcgagtt
     4741 ttggcgcttt ttctcatcga ttgacgaggc aaagggagcg agtatgcaac ctcgtaagtc
     4801 agactcagac aaagaccaac tagttattga ccagtcttct agaaggctct gttgtggatc
     4861 ttgaggccct ggagagttcc tccaacaatc cggagacaag caagtcctcg tcggactagt
     4921 cactaactag actctaacta gttgcagaca tggataatgc aaaagagaca agcaatgaag
     4981 aaagtggtaa gacatttctc ctttgtggtt ctggactagt ctttgactag tcacagtctt
     5041 aaacaaggaa aatgagcatg aaaatgagga ggaaaaggct gctgagcccg aggaagtgca
     5101 gggtgatggc agacatggta ggttaatacc ttgttagtta ttgctagtca ctgactagtc
     5161 aataactagt ctctgaacac cttgcaatca ctccgtttgc gcagctgaac agtggtgagg
     5221 ataatagtag taagttattc tagcttcaga gttataggag actagatact aactagtatt
     5281 agttgcaact aacctggatc tcagagactt tggcctcaat ctagaatcta tctagttgtc
     5341 aactagactg tggtatcatt gtcttttatt ttcctagtcc tggaactagc ttctaactag
     5401 tctccctaat atgtggctgt cttgtttttt tttttttgtt tccctacccg gatatctagt
     5461 ccccttctag gttctgttaa cctctcgggc tctgatttag tttaacgcaa acctgagatt
     5521 agtttctaac tagtctctag gttttctatc cacctttaat tgtaataata aatacaagca
     5581 acgtttatac gtcaaaagca tttataaact tttaccctaa agtagcttgc ttgtgtgttt
     5641 agtttataat tagtctctta ttaatttgat gtaggtaagc ccgccacaaa tatatatttt
     5701 taacaagata ccgtggaaaa acttcgtgct atcacaaaac agtatacaaa aaataagctt
     5761 aacaatctat tctccgcttg gtgatgctaa agggctttca atagaccttg taagtgaagg
     5821 agatggagcc gtcaatccgc taccctgcct ctggtcagtt ggtctcagca atgtaccctg
     5881 tgagtcttga tagactagtt ggtgactagt ctctgtagat ggaacaaatg gtgcttgaga
     5941 gggcacgtat ggcagagctc cagtcatctg cggaacatat actgggcccg gtacgcgtta
     6001 agcttggcac tggccgtcag ctgcttcgtc gattaaaccc tcagcgcggc cgcatttaaa
     6061 tccccacttc agaagttcct atactttcta gagaatagga acttcactat agagtcgaat
     6121 aagggcgaca ccccataatt agcccgggcg aaaggcccag tctttcgact gagcctttcg
     6181 ttttatttga tgcctggcag ttccctactc tcgcatgggg agtccccaca ctaccatcgg
     6241 cgctacggcg tttcacttct gagttcggca tggggtcagg tgggaccacc gcgctactgc
     6301 cgccaggcaa acaaggggtg ttatgagcca tattcaggta taaatgggct cgcgataatg
     6361 ttcagaattg gttaattggt tgtaacactg acccctattt gtttattttt ctaaatacat
     6421 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     6481 aggaagaata tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     6541 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     6601 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     6661 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     6721 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     6781 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     6841 agagaattat gtagcgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     6901 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     6961 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     7021 accacgatgc ctgtagcgat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     7081 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     7141 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatccgg agccggtgag
     7201 cgtggttctc gcggtatcat cgcagcgctg gggccagatg gtaagccctc ccgtatcgta
     7261 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     7321 ataggtgcct cactgattaa gcattggtaa gcagagcatt acgctgactt gacgggacgg
     7381 cgcaagctca tgaccaaaat cccttaacgt gagttacgcg cgcgtcgttc cactgagcgt
     7441 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7501 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7561 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     7621 ttctagtgta gccgtagtta gcccaccact tcaagaactc tgtagcaccg cctacatacc
     7681 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7741 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7801 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7861 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7921 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7981 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8041 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8101 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     8161 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     8221 cagtgagcga ggaagcggaa ggcgagagta gggaactgcc aggcatcaaa ctaagcagaa
     8281 ggcccctgac ggatggcctt tttgcgtttc tacaaactct ttctgtgttg taaaacgacg
     8341 gccagtctta agctcgggcc ccctgggcgg ttctgataac gagtaatcgt taatccgcaa
     8401 ataacgtaaa aacccgcttc ggcgggtttt tttatggggg gagtttaggg aaagagcatt
     8461 tgtcagaata tttaagggcg cctgtcactt tgcttgatat atgagaatta tttaacctta
     8521 taaatgagaa aaaagcaacg cactttaaat aagatacgtt gctttttcga ttgatgaaca
     8581 cctataatta aactattcat ctattattta tgattttttg tatatacaat atttctagtt
     8641 tgttaaagag aattaagaaa ataaatctcg aaaataataa agggaaaatc agtttttgat
     8701 atcaaaatta tacatgtcaa cgataataca aaatataata caaactataa gatgttatca
     8761 gtatttatta tgcatttaga ataaattttg tgtcgccctt attcgactca ctatagaagt
     8821 tcctattctc tagaaagtat aggaacttca cttcatttta tttaaatttg cggccgcgct
     8881 gaggtcttaa tgcggtagtg gggatgcctc aattgtgcta gctgcggccg ccttgctaga
     8941 tgactggtag gaatctaaca aagggaaaac cttgacgatt gggggcggaa aacatggcat
     9001 tacaggggcc aatacggacg agctcatcgt ttaatgacag gcacaatgta tgcagccttc
     9061 ctccacaaca ctcgtactgg cactacactt ttgctccaag aggaaatcat gacttgccgc
     9121 atactctggc catataagct tcccagcctt ctttctggta ccgctgtgca gcttcaaccg
     9181 ggtcgggagc agcgtagatg cctcgaccgg cgatgataaa gtcggcaccg cgtccaatag
     9241 ccgatgcagg agtctggtat tgctgtccaa gcttatctcc tttggaagag aggttcacac
     9301 ccgtcgtgaa gaccacgaaa tcttcatcct ccgaggctga agacacatcc gactgcactt
     9361 ccgtcagggc ccgcgtcgac acgaaaccca taacgaagtt cttgtatttg cgagcgtagt
     9421 caaccgatgc cttggtatac tcgcccgtag ccagcgatcc tttgggggtc atctctgcca
     9481 ggaccaacag tcctctctca ggaccatagg ggaagtcttg cgcagatgcg gtctgggcca
     9541 gagcctcgac gatgccctcg ccagggagaa cgctgcagtt gataatgtgg gcccattcgg
     9601 agatcctcag agcaccgccg tggtattgct tctggacggt attgccgatg tcgatgaatt
     9661 tgcggtcctc gaagatcaaa aagttgtact tttgagccag cacattcagg ccattgatag
     9721 tgtcgacgct gaaatcggtg aggatgtcga tgtgtgtctt gatgacggcg atgtagggac
     9781 cgagacctgt atcatcaaaa gttgattagt ccattgctag acggcatatg tattggatcc
     9841 aacagcttcc gtacggtcag cgaggtccag gagttctcgg gttgtcgtca catcagcaga
     9901 gacggtaacg tttgtcttct ttgcttcggc aatctcaaaa agtctctttg ccagaggatt
     9961 ggggtgcttg ctggctcgag caccgtaagt caattgcgac ttggacgaca tcgtgggaat
    10021 ggagggttat atgcggggta tgacggtgat tgatgagttg aagtagccga gcaatgaggt
    10081 atattatcca attgagggtg acccaaccaa atcaattgct tgagaatcaa atctcaaata
    10141 ttctaaaata gaagccgccg gggactaaat ttaaagtcgt actctatctc gatcttttcc
    10201 gcgtgtaaaa attgcgtgcc aatctggatg gagacaggcc acatcggtgc tgtattcctc
    10261 cgcctccgac tgtggggtca accacaatta aactactctg tagtcaggta cagctagaat
    10321 ggggtagaca ggcagaactg tagaagataa aacattggtc aatcactggt aactccacgt
    10381 ctagaggatc cccggccggg taccgagctc gaattcgtaa tcatgtcata ttacctagtg
    10441 ggcgcttaca cagtacacga ggagctgagg tcttaatgta ttgggatgaa ttttgtatgc
    10501 acgcgctgcg ggcgcaattt tttgaaattt tccattttta atgtcttgaa tcgcgcattg
    10561 gatcctgccc tcttgctcaa tcaaatctcg ctttgtttta cgcgcttttg gtggcatgat
    10621 ggttgttgaa agttgaggtt gataaacgcg ttggggtgga cgagaaaact accttccgcc
    10681 cgggacgcac ctaccgcccg ggatttacgt tatgactttc gcgcagaact ctgtccaggt
    10741 catgtaatat cacgtgataa gcaaggtaca cgcaaaacga ggtacattta cgaatagttt
    10801 caacattggc tttacgggaa gagctgagat taataatgaa cagcggattt gaagttcagg
    10861 aatcataatc cattgtagaa tcaactacat aacaagtcta attccatttc tttcattcgt
    10921 aaaggctttt ttaaggaagt cataacggca taaatcgaat gtccgcagta gactttcaga
    10981 ccttgcgctt cttcttggga ggggcataat ccgggacgtc gtacggatag gcgtagtcag
    11041 gcacgtcgta agggtaagcg taatcgggga catcgtaggg gtacgagcct ttcttcttct
    11101 ttgcctggcc agccttcttg gtggcggcag ggcgcttgtg cttgacggag gtctgggcgt
    11161 actcgagcca ctctttgttg gaaatggcga tcttgacctt atcgagcttc tcgtcctcag
    11221 ctttcttgaa ctggccgatg gcccagagga ccttgcgggc gatattgtag gcgccgttgg
    11281 cgtcggcgtt cttgggcaag atggcgttct cttgagcctc gtagttccgg gaatcgtaga
    11341 agataccatc cgagttcttg acaggggaga tgaggaaatc gacatcggtc cggccggtga
    11401 tggagttgcg catctggagc atcagggaca tgagggccat gaaggaggag tagaaggcct
    11461 tatcggactg ctcgcagagc agagcgcgga tgtcgccctg ctggtagttg atgccgtact
    11521 tgttgaacag ttctttgtag gcggaggtga ggcagacttc ttcccaatcg aaaacgttgt
    11581 tcttcttagg attgcggaag atgcggatgc ggttgccgta ggagtagagc ttccacttct
    11641 taatgtaatc ggcgtcggtg cggctgaagt tcttataatc cagggcgaac tcgaagagat
    11701 cttcctcggg gacgtacatg atgcgatcga agctggagat gaacttcttg gaatcggcaa
    11761 tggaggtgta cttggtcttg agcaggttca cgaagccggt cgagggatcg atcttggagg
    11821 tgagccaggc ggggatgtag aagatgaaac cgttctgggt gctcatggac ttgaacgact
    11881 cgaacttgtt agtgatctgg tagcccttga gggctccgcc ggtggcgcag gggttcgact
    11941 tcttgtcgac catgtagttg agcttgtcga tgagcatctt ctcgaacttt tggtagacct
    12001 gcttctcaac cttgacgcgg ctgttcttga agccggagtt cagatcctcg agagcgatca
    12061 cggcatcata cttctcgacc agctcgcaga tcttgtggac gacctgcgag atgtagccgg
    12121 ccttcaactc tttgatgttc tcgatggagg tccagttctg gcgggcctcg aagcgctctt
    12181 tttctttctt atcgaggagg ctgtggtagt cggtcttgat gcggataccg ttgaagttgt
    12241 tgatgatttc gttcagggag tactgctcga cgatgttacc cttgccatcg acgacgacga
    12301 tgtagaggag gttgcgctcg ccgcgatcga taccgatgac gtaggggttg tcatcgtgct
    12361 tcaggaggac gcggacctcg gtattgatct tgaagatgtt cttagggcac ttgttgatgg
    12421 cgatagggat gtgcagctcg tactgatcct cgctgaagcg cttgtccttg tagacgtcgt
    12481 aggacaaggt ggtagttttc ttggggttat caggattctt gttggcgatc ggggagttag
    12541 caggatggac gaccaactct tctttcttca ggctagcacg gcgcatgaac agctcggcac
    12601 cgccggagag gcggatctgg ccgtggttgt tctcgtcgaa cagcagcttg aagtacatgg
    12661 tgtggaggtt aggggtgccg tgggacttat cggagaaatc cttgttgtag atttggaaca
    12721 tgtagagctt gccctcttcg acgagcttat cgacctcttt cttggaggcg gactcgaagg
    12781 acaccttgta accctgttcc tcgacctcac ggtagaagcc agcgatatcc ttatacttct
    12841 cagtctcgga gaagttgaaa tcgtaggcgt tggaccactt ggggtagcgc gagatggaat
    12901 ccttgaagaa atcgatgagc ttgtggcaat cgttgaggtt gaacatatcg ccctttttga
    12961 aggtgccgtt cttatagatc ttctggatat cctcggaggg gttgtagtag gccatccatt
    13021 tcttgctgaa gaacaccttg ggcagcatct tgttaggacc ggggagcagc ttgtagttga
    13081 tcttttcgta gttgccgttg acatcgtcct tatcgatctt ttggaggcac ttggcgtatt
    13141 tcttatccat gatggcgagg tagtacttgc tgccgtagcg gaggatggtg gcgcggtaat
    13201 cggtttcttt gtctttgtcc cagccgccca tgaactgagg attctggaag tacaacttga
    13261 acttatcctt ggagtaaggc ttctgggtga cgtagttgcg gatagcatcg tagatgtgat
    13321 cgaccttgag gaggatatcg taggccagga cgaaatcacc gtagaagctc tcgtcgcggt
    13381 tagtctcttt gccctcaccg aagaaagcct tgatgtagtt ctcgaacgac ttcacggaat
    13441 cgagcagatc cttcatgata gcgacgacgg cgtcgttctt cttcagggac ttttcgagga
    13501 cgaagtcggc atcgaacagc ttctcagagg agccgtagac cttgtagatc tcgtccacct
    13561 tctggatgat gatctctttc agcttctcga cgacggagag atcggcatcg gcgtactctt
    13621 ggagctgctc gaggctgaag gagccgatct tcttgaagga cttgcggcga tcatcctcgt
    13681 acttctcggt aacgacggcc ttcttcttga ggtggatgtc atcgtactcg gcgttccact
    13741 tatcgcggat gacgttccac tcgccgaaga tgtccttgga gatggtggag atagcagggc
    13801 cgttcttgac gaagatgccg gcggaggagt actcatcgaa gttcttgaaa agcttctcca
    13861 acttcttgat gctggagaag atctcggagt tcttgttgag ggtgttgcga aagacctcga
    13921 ggacttcctc atcggaggtg taaccctcgc cgtagaagga cagggactcg cgatcggaga
    13981 ggacctgctt gtagaggggc ttgaacttgg ggagcttctg cttggtcttt tgattgtaga
    14041 ggttgatgta ctcgttgagg cccttgatct tctcgccgga ctcggtgacg aagccgccga
    14101 tgatggcgtt gtagacatcg atgccctctt gggtgagcac gaagttgaag aactcgccct
    14161 cgaagaaatc ctcgacatcg taatcggagt tgaggatctt ttctttgatc tcttggacct
    14221 cgtgcttgtc gaagatggca tcgaccttct cgaagatatc catgttgctg atgtagcggg
    14281 tcaagttctc gttgatgcag cggaaggcga tagaggtgga cttggcttcc tcggagaaca
    14341 tgttttcgcg gttatcgaag aaaccggtga aggcggtagt gaagccgttg aaggagttga
    14401 cgagggcgat ctcatcctta tcatcgagga actcaggcag gatggtttcg atgatatcct
    14461 tcttgaacag ggacttgtag ccctcgttgc ccttgaaggc cttggcgatc tctttgcgga
    14521 ggttgatctc gaggttttcc agctctttgt tctctttctc ggtgcgggtt ttcttgcgga
    14581 acagggagat gtagttgttg aggttcttga gcttgatgga gtggaggaca tcgttgatga
    14641 aggagaggta gtagcgatcg aggagcttct tgacgccctt gtaatcctcg gcgcgcttct
    14701 catcctcgac gaggaggcgc ttgttatcga tattctcttg ggtcttgccg acggggatag
    14761 ccttgaagcg gagggtcttg ctgagggagt agcagttggt gaacttctcg agcttggaca
    14821 tacgaagtct gtgttatgtt ttgtggaata taaaagggag aagtgctcag atactacggc
    14881 tagtaatgcg tagaggtgct gagcagagaa gttagcagga gatggatgaa agaaggatgg
    14941 aggagagaag cgagggaagg gaagatatac gtactgaagc ggacaaggag aggaagggaa
    15001 agaaaatttt gtgggagggg gaggggaaat ccttttatgg aggggcaagc gaggacgacg
    15061 actaagcggg cgggctcaaa cccttggctg gtagatatct gtcattggat atggaggggt
    15121 aacccgcccc actcccgatg caaattcttc tcctagactc ccaccttggg cgtcgagcct
    15181 ttacaacacc ttccggaacg gcagcccagc gcgactcttt ccttggttct gctccacccc
    15241 atctcgtttt catggcttag gggcagaaat ttttttttgt ttagcttttc tttacttgct
    15301 atcgcctcta cggtggattc tcggatcgaa aaagctgggc atcaacgcgt tgatggtcgc
    15361 atggcggctt ttatcactct gtactttgca gcggacaacc ccgcggctgt acgagtttgc
    15421 cgccttttaa tttgtctgcg accagcctgc gatggagcga gcgagtggcg ttgcttgatc
    15481 cacagcgtca agatcaa
//