p(+)MV-NSE-FlagP-F497A-3p

LOCUS       p(+)MV-NSE-FlagP-F497A-3p      19043 bp DNA     circular SYN 26-AUG-2024
DEFINITION  Expresses recombinant measles virus with Flag-P-F497A gene tagged 
            si2 (siP) target sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19043)
  TITLE     biG-biS plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 26, 2024 from SnapGene Server 7.0.3
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
FEATURES             Location/Qualifiers
     source          1..19043
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1213..1221
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             1810..1833
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2853..2864
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             10038..10049
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(16136..16154)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      16150..16197
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(16250..16268)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(16289..16305)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16289..16305)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(16302..16324)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    16313..16329
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(16337..16367)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16382..16403
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(16520..16537)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(16691..17279)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16771..16790)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(17450..18310)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     18073..18092
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(18311..18415)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(18578..18599)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     18989..19011
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     19003..19020
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     19004..19020
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 accaaacaaa gttgggtaag gatagttcaa tcaatgatca tcttctagtg cacttaggat
       61 tcaagatcct attatcaggg acaagagcag gattagggat atccgagatg gccacacttt
      121 taaggagctt agcattgttc aaaagaaaca aggacaaacc acccattaca tcaggatccg
      181 gtggagccat cagaggaatc aaacacatta ttatagtacc aatccctgga gattcctcaa
      241 ttaccactcg atccagactt ctggaccggt tggtcaggtt aattggaaac ccggatgtga
      301 gcgggcccaa actaacaggg gcactaatag gtatattatc cttatttgtg gagtctccag
      361 gtcaattgat tcagaggatc accgatgacc ctgacgttag cataaggctg ttagaggttg
      421 tccagagtga ccagtcacaa tctggcctta ccttcgcatc aagaggtacc aacatggagg
      481 atgaggcgga ccaatacttt tcacatgatg atccaattag tagtgatcaa tccaggttcg
      541 gatggttcga gaacaaggaa atctcagata ttgaagtgca agaccctgag ggattcaaca
      601 tgattctggg taccatccta gcccaaattt gggtcttgct cgcaaaggcg gttacggccc
      661 cagacacggc agctgattcg gagctaagaa ggtggataaa gtacacccaa caaagaaggg
      721 tagttggtga atttagattg gagagaaaat ggttggatgt ggtgaggaac aggattgccg
      781 aggacctctc cttacgccga ttcatggtcg ctctaatcct ggatatcaag agaacacccg
      841 gaaacaaacc caggattgct gaaatgatat gtgacattga tacatatatc gtagaggcag
      901 gattagccag ttttatcctg actattaagt ttgggataga aactatgtat cctgctcttg
      961 gactgcatga atttgctggt gagttatcca cacttgagtc cttgatgaac ctttaccagc
     1021 aaatggggga aactgcaccc tacatggtaa tcctggagaa ctcaattcag aacaagttca
     1081 gtgcaggatc ataccctctg ctctggagct atgccatggg agtaggagtg gaacttgaaa
     1141 actccatggg aggtttgaac tttggccgat cttactttga tccagcatat tttagattag
     1201 ggcaagagat ggtaaggagg tcagctggaa aggtcagttc cacattggca tctgaactcg
     1261 gtatcactgc cgaggatgca aggcttgttt cagagattgc aatgcatact actgaggaca
     1321 agatcagtag agcggttgga cccagacaag cccaagtatc atttctacac ggtgatcaaa
     1381 gtgagaatga gctaccgaga ttggggggca aggaagatag gagggtcaaa cagagtcgag
     1441 gagaagccag ggagagctac agagaaaccg ggcccagcag agcaagtgat gcgagagctg
     1501 cccatcttcc aaccggcaca cccctagaca ttgacactgc atcggagtcc agccaagatc
     1561 cgcaggacag tcgaaggtca gctgacgccc tgcttaggct gcaagccatg gcaggaatct
     1621 cggaagaaca aggctcagac acggacaccc ctatagtgta caatgacaga aatcttctag
     1681 actaggtgcg agaggccgag gaccagaaca acatccgcct accctccatc attgttataa
     1741 aaaacttagg aaccaggtcc acacagccgc cagcccatca accatccact cccacgattg
     1801 gagcagatgg attataagga tgacgatgac aaagcagaag agcaggcacg ccatgtcaaa
     1861 aacggactgg aatgcatccg ggctctcaag gccgagccca tcggctcact ggccatcgag
     1921 gaagctatgg cagcatggtc agaaatatca gacaacccag gacaggagcg agccacctgc
     1981 agggaagaga aggcaggcag ttcgggtctc agcaaaccat gcctctcagc aattggatca
     2041 actgaaggcg gtgcacctcg catccgcggt cagggacctg gagagagcga tgacgacgct
     2101 gaaactttgg gaatcccccc aagaaatctc caggcatcaa gcactgggct acagtgtcat
     2161 tatgtttatg atcacagcgg tgaagcggtt aagggaatcc aagatgctga ctctatcatg
     2221 gttcaatcag gccttgatgg tgatagcacc ctctcaggag gagacaatga atctgaaaac
     2281 agcgatgtgg atattggcga acctgatacc gagggatatg ctatcactga ccggggatct
     2341 gctcccatct ctatggggtt cagggcttct gatgttgaaa ctgcagaagg aggggagatc
     2401 cacgagctcc tgagactcca atccagaggc aacaactttc cgaagcttgg gaaaactctc
     2461 aatgttcctc cgcccccgga ccccggtagg gccagcactt ccgggacacc cattaaaaag
     2521 ggcacagacg cgagattagc ctcatttgga acggagatcg cgtcttcatt gacaggtggt
     2581 gcaacccaat gtgctcgaaa gtcaccctcg gaaccatcag ggccaggtgc acctgcgggg
     2641 aacgtccccg agtgtgtgag caatgccgca ctgatacagg agtggacacc cgaatctggc
     2701 accacaatct ccccgagatc ccagaataat gaagaagggg gagaccatta tgatgatgag
     2761 ctgttctctg atgtccaaga tattaaaaca gccttggcca aaatacacga ggataatcag
     2821 aagataatct ccaagctaga atcactgctg ttattgaagg gagaagttga gtcaattaag
     2881 aagcagatca acaggcaaaa tatcagcata tccaccctgg aaggacacct ctcaagcatc
     2941 atgatcgcca ttcctggact tgggaaggat cccaacgacc ccactgcaga tgtcgaaatc
     3001 aatcccgact tgaaacccat cataggcaga gattcaggcc gagcactggc cgaagttctc
     3061 aagaaacccg ttgcctcgcg acaactccaa ggaatgacaa atggacggac cagttccaga
     3121 ggacagctgc tgaaggaatt tcagctaaag ccgatcggga aaaagatgag ctcagccgtc
     3181 gggtttgttc ctgacaccgg ccctgcatca cgcagtgtaa tccgctccat tataaaatcc
     3241 agccggctag aggaggatcg gaagcgttac ctgatgactc tccttgatga tatcaaagga
     3301 gccaatgatc ttgccaaggc ccaccagatg ctgatgaaga taataatgaa gtagctacag
     3361 ctcaacttac ctgccaaccg gacacctctc aagcatcatc catgcggaca cctctcaagc
     3421 atcatcagtc gggacacctc tcaagcatca tacccaacta gcgtaccatg acgtcctagt
     3481 acaacctaaa tccattataa aaaacttagg agcaaagtga ttgcctccca agttccacaa
     3541 tgacagagat ctacgacttc gacaagtcgg catgggacat caaagggtcg atcgctccga
     3601 tacaacccac cacctacagt gatggcaggc tggtgcccca ggtcagagtc atagatcctg
     3661 gtctaggcga caggaaggat gaatgcttta tgtacatgtt tctgctgggg gttgttgagg
     3721 acagcgattc cctagggcct ccaatcgggc gagcatttgg gtccctgccc ttaggtgttg
     3781 gcagatccac agcaaagccc gaaaaactcc tcaaagaggc cactgagctt gacatagttg
     3841 ttagacgtac agcagggctc aatgaaaaac tggtgttcta caacaacacc ccactaactc
     3901 tcctcacacc ttggagaaag gtcctaacaa cagggagtgt cttcaacgca aaccaagtgt
     3961 gcaatgcggt taatctgata ccgctcgata ccccgcagag gttccgtgtt gtttatatga
     4021 gcatcacccg tctttcggat aacgggtatt acaccgttcc tggaagaatg ctggaattca
     4081 gatcggtcaa tgcagtggcc ttcaacctgc tggtgaccct taggattgac aaggcgatag
     4141 gccctgggaa gatcatcgac aatacagagc aacttcctga ggcaacattt atggtccaca
     4201 tcgggaactt caggagaaag aagagtgaag tctactctgc cgattattgc aaaatgaaaa
     4261 tcgaaaagat gggcctggtt tttgcacttg gtgggatagg gggcaccagt cttcacatta
     4321 gaagcacagg caaaatgagc aagactctcc atgcacaact cgggttcaag aagaccttat
     4381 gttacccgct gatggatatc aatgaagacc ttaatcgatt actctggagg agcagatgca
     4441 agatagtaag aatccaggca gttttgcagc catcagttcc tcaagaattc cgcatttacg
     4501 acgacgtgat cataaatgat gaccaaggac tattcaaagt tctgtagacc gtagtgccca
     4561 gcaatgcccg aaaacgaccc ccctcacaat gacagccaga aggcccggac aaaaaagccc
     4621 cctccgaaag actccacgga ccaagcgaga ggccagccag cagccgacgg caagcgcgaa
     4681 caccaggcgg ccccagcaca gaacagccct gacacaaggc caccaccagc caccccaatc
     4741 tgcatcctcc tcgtgggacc cccgaggacc aacccccaag gctgcccccg atccaaacca
     4801 ccaaccgcat ccccaccacc cccgggaaag aaacccccag caattggaag gcccctcccc
     4861 ctcttcctca acacaagaac tccacaaccg aaccgcacaa gcgaccgagg tgacccaacc
     4921 gcaggcatcc gactccctag acagatcctc tctccccggc aaactaaaca aaacttaggg
     4981 ccaaggaaca tacacaccca acagaaccca gaccccggcc cacggcgccg cgcccccaac
     5041 ccccgacaac cagagggagc ccccaaccaa tcccgccggc tcccccggtg cccacaggca
     5101 gggacaccaa cccccgaaca gacccagcac ccaaccatcg acaatccaag acgggggggc
     5161 ccccccaaaa aaaggccccc aggggccgac agccagcacc gcgaggaagc ccacccaccc
     5221 cacacacgac cacggcaacc aaaccagaac ccagaccacc ctgggccacc agctcccaga
     5281 ctcggccatc accccgcaga aaggaaaggc cacaacccgc gcaccccagc cccgatccgg
     5341 cggggagcca cccaacccga accagcaccc aagagcgatc cccgaaggac ccccgaaccg
     5401 caaaggacat cagtatccca cagcctctcc aagtcccccg gtctcctcct cttctcgaag
     5461 ggaccaaaag atcaatccac cacacccgac gacactcaac tccccacccc taaaggagac
     5521 accgggaatc ccagaatcaa gactcatcca atgtccatca tgggtctcaa ggtgaacgtc
     5581 tctgccatat tcatggcagt actgttaact ctccaaacac ccaccggtca aatccattgg
     5641 ggcaatctct ctaagatagg ggtggtagga ataggaagtg caagctacaa agttatgact
     5701 cgttccagcc atcaatcatt agtcataaaa ttaatgccca atataactct cctcaataac
     5761 tgcacgaggg tagagattgc agaatacagg agactactga gaacagtttt ggaaccaatt
     5821 agagatgcac ttaatgcagt gacccagaat ataagaccgg ttcagagtgt agcttcaagt
     5881 aggagacaca agagatttgc gggagtagtc ctggcaggtg cggccctagg cgttgccaca
     5941 gctgctcaga taacagccgg cattgcactt caccagtcca tgctgaactc tcaagccatc
     6001 gacaatctga gagcgagcct ggaaactact aatcaggcaa ttgaggcaat cagacaagca
     6061 gggcaggaga tgatattggc tgttcagggt gtccaagact acatcaataa tgagctgata
     6121 ccgtctatga accaactatc ttgtgattta atcggccaga agctcgggct caaattgctc
     6181 agatactata cagaaatcct gtcattattt ggccccagct tacgggaccc catatctgcg
     6241 gagatatcta tccaggcttt gagctatgcg cttggaggag acatcaataa ggtgttagaa
     6301 aagctcggat acagtggagg tgatttactg ggcatcttag agagcagagg aataaaggcc
     6361 cggataactc acgtcgacac agagtcctac ttcattgtcc tcagtatagc ctatccgacg
     6421 ctgtccgaga ttaagggggt gattgtccac cggctagagg gggtctcgta caacataggc
     6481 tctcaagagt ggtataccac tgtgcccaag tatgttgcaa cccaagggta ccttatctcg
     6541 aattttgatg agtcatcgtg tactttcatg ccagagggga ctgtgtgcag ccaaaatgcc
     6601 ttgtacccga tgagtcctct gctccaagaa tgcctccggg ggtccaccaa gtcctgtgct
     6661 cgtacactcg tatccgggtc ttttgggaac cggttcattt tatcacaagg gaacctaata
     6721 gccaattgtg catcaatcct ttgcaagtgt tacacaacag gaacgatcat taatcaagac
     6781 cctgacaaga tcctaacata cattgctgcc gatcactgcc cggtagtcga ggtgaacggc
     6841 gtgaccatcc aagtcgggag caggaggtat ccagacgctg tgtacttgca cagaattgac
     6901 ctcggtcctc ccatatcatt ggagaggttg gacgtatgga caaatctggg gaatgcaatt
     6961 gctaagttgg aggatgccaa ggaattgttg gagtcatcgg accagatatt gaggagtatg
     7021 aaaggtttat cgagcactag catagtctac atcctgattg cagtgtgtct tggagggttg
     7081 atagggatcc ccgctttaat atgttgctgc agggggcgtt gtaacaaaaa gggagaacaa
     7141 gttggtatgt caagaccagg cctaaagcct gatcttacgg gaacatcaaa atcctatgta
     7201 aggtcgctct gatcctctac aactcttgaa acacaaatgt cccacaagtc tcctcttcgt
     7261 catcaagcaa ccaccgcacc cagcatcaag cccacctgaa attatctccg gtttccctct
     7321 ggccgaacaa tatcggtagt taattaaaac ttagggtgca agatcatcca caatgtcacc
     7381 acaacgagac cggataaatg ccttctacaa agataacccc catcccaagg gaagtaggat
     7441 agtcattaac agagaacatc ttatgattga tagaccttat gttttgctgg ctgttctgtt
     7501 tgtcatgttt ctgagcttga tcgggttgct agccattgca ggcattagac ttcatcgggc
     7561 agccatctac accgcagaga tccataaaag cctcagcacc aatctagatg taactaactc
     7621 aatcgagcat caggtcaagg acgtgctgac accactcttc aaaatcatcg gtgatgaagt
     7681 gggcctgagg acacctcaga gattcactga cctagtgaaa ttcatctctg acaagattaa
     7741 attccttaat ccggataggg agtacgactt cagagatctc acttggtgta tcaacccgcc
     7801 agagagaatc aaattggatt atgatcaata ctgtgcagat gtggctgctg aagagctcat
     7861 gaatgcattg gtgaactcaa ctctactgga gaccagaaca accaatcagt tcctagctgt
     7921 ctcaaaggga aactgctcag ggcccactac aatcagaggt caattctcaa acatgtcgct
     7981 gtccctgtta gacttgtatt taggtcgagg ttacaatgtg tcatctatag tcactatgac
     8041 atcccaggga atgtatgggg gaacttacct agtggaaaag cctaatctga gcagcaaaag
     8101 gtcagagttg tcacaactga gcatgtaccg agtgtttgaa gtaggtgtta tcagaaatcc
     8161 gggtttgggg gctccggtgt tccatatgac aaactatctt gagcaaccag tcagtaatga
     8221 tctcagcaac tgtatggtgg ctttggggga gctcaaactc gcagcccttt gtcacgggga
     8281 agattctatc acaattccct atcagggatc agggaaaggt gtcagcttcc agctcgtcaa
     8341 gctaggtgtc tggaaatccc caaccgacat gcaatcctgg gtccccttat caacggatga
     8401 tccagtgata gacaggcttt acctctcatc tcacagaggt gttatcgctg acaaccaagc
     8461 aaaatgggct gtcccgacaa cacgaacaga tgacaagttg cgaatggaga catgcttcca
     8521 acaggcgtgt aagggtaaaa tccaagcact ctgcgagaat cccgagtggg caccattgaa
     8581 ggataacagg attccttcat acggggtctt gtctgttgat ctgagtctga cagttgagct
     8641 taaaatcaaa attgcttcgg gattcgggcc attgatcaca cacggttcag ggatggacct
     8701 atacaaatcc aaccacaaca atgtgtattg gctgactatc ccgccaatga agaacctagc
     8761 cttaggtgta atcaacacat tggagtggat accgagattc aaggttagtc cctacctctt
     8821 cactgtccca attaaggaag caggcggaga ctgccatgcc ccaacatacc tacctgcgga
     8881 ggtggatggt gatgtcaaac tcagttccaa tctggtgatt ctacctggtc aagatctcca
     8941 atatgttttg gcaacctacg atacttccag ggttgaacat gctgtggttt attacgttta
     9001 cagcccaagc cgctcatttt cttactttta tccttttagg ttgcctataa agggggtccc
     9061 catcgaatta caagtggaat gcttcacatg ggaccaaaaa ctctggtgcc gtcacttctg
     9121 tgtgcttgcg gactcagaat ctggtggaca tatcactcac tctgggatgg tgggcatggg
     9181 agtcagctgc acagtcaccc gggaagatgg aaccaatcgc agatagggct gctagtgaac
     9241 caatcacatg atgtcaccca gacatcaggc atacccacta gtgtgaaata gacatcagaa
     9301 ttaagaaaaa cgtagggtcc aagtggttcc ccgttatgga ctcgctatct gtcaaccaga
     9361 tcttataccc tgaagttcac ctagatagcc cgatagttac caataagata gtagccatcc
     9421 tggagtatgc tcgagtccct cacgcttaca gcctggagga ccctacactg tgtcagaaca
     9481 tcaagcaccg cctaaaaaac ggattttcca accaaatgat tataaacaat gtggaagttg
     9541 ggaatgtcat caagtccaag cttaggagtt atccggccca ctctcatatt ccatatccaa
     9601 attgtaatca ggatttattt aacatagaag acaaagagtc aacgaggaag atccgtgaac
     9661 tcctcaaaaa ggggaattcg ctgtactcca aagtcagtga taaggttttc caatgcttaa
     9721 gggacactaa ctcacggctt ggcctaggct ccgaattgag ggaggacatc aaggagaaag
     9781 ttattaactt gggagtttac atgcacagct cccagtggtt tgagcccttt ctgttttggt
     9841 ttacagtcaa gactgagatg aggtcagtga ttaaatcaca aacccatact tgccatagga
     9901 ggagacacac acctgtattc ttcactggta gttcagttga gttgctaatc tctcgtgacc
     9961 ttgttgctat aatcagtaaa gagtctcaac atgtatatta cctgacattt gaactggttt
    10021 tgatgtattg tgatgtcata gaggggaggt taatgacaga gaccgctatg actattgatg
    10081 ctaggtatac agagcttcta ggaagagtca gatacatgtg gaaactgata gatggtttct
    10141 tccctgcact cgggaatcca acttatcaaa ttgtagccat gctggagcct ctttcacttg
    10201 cttacctgca gctgagggat ataacagtag aactcagagg tgctttcctt aaccactgct
    10261 ttactgaaat acatgatgtt cttgaccaaa acgggttttc tgatgaaggt acttatcatg
    10321 agttaattga agctctagat tacattttca taactgatga catacatctg acaggggaga
    10381 ttttctcatt tttcagaagt ttcggccacc ccagacttga agcagtaacg gctgctgaaa
    10441 atgttaggaa atacatgaat cagcctaaag tcattgtgta tgagactctg atgaaaggtc
    10501 atgccatatt ttgtggaatc ataatcaacg gctatcgtga caggcacgga ggcagttggc
    10561 caccgctgac cctccccctg catgctgcag acacaatccg gaatgctcaa gcttcaggtg
    10621 atgggttaac acatgagcag tgcgttgata actggaaatc ttttgctgga gtgaaatttg
    10681 gctgctttat gcctcttagc ctggatagtg atctgacaat gtacctaaag gacaaggcac
    10741 ttgctgctct ccaaagggaa tgggattcag tttacccgaa agagttcctg cgttacgacc
    10801 ctcccaaggg aaccgggtca cggaggcttg tagatgtttt ccttaatgat tcgagctttg
    10861 acccatatga tgtgataatg tatgttgtaa gtggagctta cctccatgac cctgagttca
    10921 acctgtctta cagcctgaaa gaaaaggaga tcaaggaaac aggtagactt tttgctaaaa
    10981 tgacttacaa aatgagggca tgccaagtga ttgctgaaaa tctaatctca aacgggattg
    11041 gcaaatattt taaggacaat gggatggcca aggatgagca cgatttgact aaggcactcc
    11101 acactctagc tgtctcagga gtccccaaag atctcaaaga aagtcacagg ggggggccag
    11161 tcttaaaaac ctactcccga agcccagtcc acacaagtac caggaacgtg agagcagcaa
    11221 aagggtttat agggttccct caagtaattc ggcaggacca agacactgat catccggaga
    11281 atatggaagc ttacgagaca gtcagtgcat ttatcacgac tgatctcaag aagtactgcc
    11341 ttaattggag atatgagacc atcagcttgt ttgcacagag gctaaatgag atttacggat
    11401 tgccctcatt tttccagtgg ctgcataaga ggcttgagac ctctgtcctg tatgtaagtg
    11461 accctcattg cccccccgac cttgacgccc atatcccgtt atataaagtc cccaatgatc
    11521 aaatcttcat taagtaccct atgggaggta tagaagggta ttgtcagaag ctgtggacca
    11581 tcagcaccat tccctatcta tacctggctg cttatgagag cggagtaagg attgcttcgt
    11641 tagtgcaagg ggacaatcag accatagccg taacaaaaag ggtacccagc acatggccct
    11701 acaaccttaa gaaacgggaa gctgctagag taactagaga ttactttgta attcttaggc
    11761 aaaggctaca tgatattggc catcacctca aggcaaatga gacaattgtt tcatcacatt
    11821 tttttgtcta ttcaaaagga atatattatg atgggctact tgtgtcccaa tcactcaaga
    11881 gcatcgcaag atgtgtattc tggtcagaga ctatagttga tgaaacaagg gcagcatgca
    11941 gtaatattgc tacaacaatg gctaaaagca tcgagagagg ttatgaccgt taccttgcat
    12001 attccctgaa cgtcctaaaa gtgatacagc aaattctgat ctctcttggc ttcacaatca
    12061 attcaaccat gacccgggat gtagtcatac ccctcctcac aaacaacgac ctcttaataa
    12121 ggatggcact gttgcccgct cctattgggg ggatgaatta tctgaatatg agcaggctgt
    12181 ttgtcagaaa catcggtgat ccagtaacat catcaattgc tgatctcaag agaatgattc
    12241 tcgcctcact aatgcctgaa gagaccctcc atcaggtaat gacacaacaa ccgggggact
    12301 cttcattcct agactgggct agcgaccctt actcagcaaa tcttgtatgt gtccagagca
    12361 tcactagact cctcaagaac ataactgcaa ggtttgtcct gatccatagt ccaaacccaa
    12421 tgttaaaagg attattccat gatgacagta aagaagagga cgagggactg gcggcattcc
    12481 tcatggacag gcatattata gtacctaggg cagctcatga aatcctggat catagtgtca
    12541 caggggcaag agagtctatt gcaggcatgc tggataccac aaaaggcttg attcgagcca
    12601 gcatgaggaa ggggggttta acctctcgag tgataaccag attgtccaat tatgactatg
    12661 aacaattcag agcagggatg gtgctattga caggaagaaa gagaaatgtc ctcattgaca
    12721 aagagtcatg ttcagtgcag ctggcgagag ctctaagaag ccatatgtgg gcgaggctag
    12781 ctcgaggacg gcctatttac ggccttgagg tccctgatgt actagaatct atgcgaggcc
    12841 accttattcg gcgtcatgag acatgtgtca tctgcgagtg tggatcagtc aactacggat
    12901 ggttttttgt cccctcgggt tgccaactgg atgatattga caaggaaaca tcatccttga
    12961 gagtcccata tattggttct accactgatg agagaacaga catgaagctt gccttcgtaa
    13021 gagccccaag tcgatccttg cgatctgctg ttagaatagc aacagtgtac tcatgggctt
    13081 acggtgatga tgatagctct tggaacgaag cctggttgtt ggctaggcaa agggccaatg
    13141 tgagcctgga ggagctaagg gtgatcactc ccatctcaac ttcgactaat ttagcgcata
    13201 ggttgaggga tcgtagcact caagtgaaat actcaggtac atcccttgtc cgagtggcga
    13261 ggtataccac aatctccaac gacaatctct catttgtcat atcagataag aaggttgata
    13321 ctaactttat ataccaacaa ggaatgcttc tagggttggg tgttttagaa acattgtttc
    13381 gactcgagaa agataccgga tcatctaaca cggtattaca tcttcacgtc gaaacagatt
    13441 gttgcgtgat cccgatgata gatcatccca ggatacccag ctcccgcaag ctagagctga
    13501 gggcagagct atgtaccaac ccattgatat atgataatgc acctttaatt gacagagatg
    13561 caacaaggct atacacccag agccatagga ggcaccttgt ggaatttgtt acatggtcca
    13621 caccccaact atatcacatt ttagctaagt ccacagcact atctatgatt gacctggtaa
    13681 caaaatttga gaaggaccat atgaatgaaa tttcagctct cataggggat gacgatatca
    13741 atagtttcat aactgagttt ctgctcatag agccaagatt attcactatc tacttgggcc
    13801 agtgtgcggc catcaattgg gcatttgatg tacattatca tagaccatca gggaaatatc
    13861 agatgggtga gctgttgtca tcgttccttt ctagaatgag caaaggagtg tttaaggtgc
    13921 ttgtcaatgc tctaagccac ccaaagatct acaagaaatt ctggcattgt ggtattatag
    13981 agcctatcca tggtccttca cttgatgctc aaaacttgca cacaactgtg tgcaacatgg
    14041 tttacacatg ctatatgacc tacctcgacc tgttgttgaa tgaagagtta gaagagttca
    14101 catttctctt gtgtgaaagc gacgaggatg tagtaccgga cagattcgac aacatccagg
    14161 caaaacactt atgtgttctg gcagatttgt actgtcaacc agggacctgc ccaccaattc
    14221 aaggtctaag accggtagag aaatgtgcag ttctaaccga ccatatcaag gcagaggcta
    14281 tgttatctcc agcaggatct tcgtggaaca taaatccaat tattgtagac cattactcat
    14341 gctccctgac ttatctccgg cgaggatcga tcaaacagat aagattgaga gttgatccag
    14401 gattcatttt cgacgccctc gctgaggtaa atgtcagtca gccaaagatc ggcagcaaca
    14461 acatctcaaa tatgagcatc aaggctttca gacccccaca cgatgatgtt gcaaaattgc
    14521 tcaaagatat caacacaagc aagcacaatc ttcccatttc agggggcaat ctcgccaatt
    14581 atgaaatcca tgctttccgc agaatcgggt tgaactcatc tgcttgctac aaagctgttg
    14641 agatatcaac attaattagg agatgccttg agccagggga ggacggcttg ttcttgggtg
    14701 agggatcggg ttctatgttg atcacttata aggagatact taaactaagc aagtgcttct
    14761 ataatagtgg ggtttccgcc aattctagat ctggtcaaag ggaattagca ccctatccct
    14821 ccgaagttgg ccttgtcgaa cacagaatgg gagtaggtaa tattgtcaaa gtgctcttta
    14881 acgggaggcc cgaagtcacg tgggtaggca gtgtagattg cttcaatttc atagttagta
    14941 atatccctac ctctagtgtg gggtttatcc attcagatat agagaccttg cctgacaaag
    15001 atactataga gaagctagag gaattggcag ccatcttatc gatggctctg ctcctgggca
    15061 aaataggatc aatactggtg attaagctta tgcctttcag cggggatttt gttcagggat
    15121 ttataagtta tgtagggtct cattatagag aagtgaacct tgtataccct agatacagca
    15181 acttcatatc tactgaatct tatttggtta tgacagatct caaggctaac cggctaatga
    15241 atcctgaaaa gattaagcag cagataattg aatcatctgt gaggacttca cctggactta
    15301 taggtcacat cctatccatt aagcaactaa gctgcataca agcaattgtg ggagacgcag
    15361 ttagtagagg tgatatcaat cctactctga aaaaacttac acctatagag caggtgctga
    15421 tcaattgcgg gttggcaatt aacggaccta agctgtgcaa agaattgatc caccatgatg
    15481 ttgcctcagg gcaagatgga ttgcttaatt ctatactcat cctctacagg gagttggcaa
    15541 gattcaaaga caaccaaaga agtcaacaag ggatgttcca cgcttacccc gtattggtaa
    15601 gtagcaggca acgagaactt atatctagga tcacccgcaa attttggggg cacattcttc
    15661 tttactccgg gaacagaaag ttgataaata agtttatcca gaatctcaag tccggctatc
    15721 tgatactaga cttacaccag aatatcttcg ttaagaatct atccaagtca gagaaacaga
    15781 ttattatgac ggggggtttg aaacgtgagt gggtttttaa ggtaacagtc aaggagacca
    15841 aagaatggta taagttagtc ggatacagtg ccctgattaa ggactaattg gttgaactcc
    15901 ggaaccctaa tcctgcccta ggtggttagg cattatttgc aatatattaa agaaaacttt
    15961 gaaaatacga agtttctatt cccagctttg tctggtggcc ggcatggtcc cagcctcctc
    16021 gctggcgctg gctgggcaac attccgaggg gaccgtcccc tcggtaatgg cgaatgggac
    16081 gcggccgatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
    16141 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
    16201 aaaggaggaa ctatatccgg atgcggccgc aggtacccag cttttgttcc ctttagtgag
    16261 ggttaatttc gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
    16321 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
    16381 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
    16441 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
    16501 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    16561 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    16621 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    16681 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    16741 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    16801 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    16861 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    16921 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    16981 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    17041 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    17101 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
    17161 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    17221 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    17281 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    17341 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
    17401 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    17461 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    17521 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    17581 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    17641 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    17701 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    17761 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    17821 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    17881 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    17941 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    18001 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    18061 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    18121 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    18181 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    18241 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    18301 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    18361 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    18421 ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
    18481 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
    18541 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
    18601 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
    18661 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
    18721 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg ccatttaggc
    18781 catagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
    18841 aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg
    18901 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
    18961 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
    19021 gaattgtaat acgactcact ata
//