pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SHANK3-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SHANK3-FLAG             8106 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 16, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..4819
                     /codon_start=1
                     /gene="SHANK3"
                     /gene_synonym="DEL22q13.3; PROSAP2; PSAP2; SCZD15;SPANK-2"
                     /product="SH3 and multiple ankyrin repeat domains protein3"
                     /label=SHANK3
                     /note="shank postsynaptic density protein; proline 
                     richsynapse associated protein 2; shank3 postsynaptic 
                     density protein"
                     /db_xref="GeneID:85358"
                     /db_xref="HGNC:HGNC:14294"
                     /db_xref="MIM:606230"
                     /protein_id="NP_001358973.1"
                     /translation="VGHKQVVALIRQGGNRLVMKVVSVTRKPEEDGARRRAPPPPKRAP
                     STTLTLRSKSMTAELEELASIRRRKGEKLDEMLAAAAEPTLRPDIADADSRAATVKQRP
                     TSRRITPAEISSLFERQGLPGPEKLPGSLRKGIPRTKSVGEDEKLASLLEGRFPRSTSM
                     QDPVREGRGIPPPPQTAPPPPPAPYYFDSGPPPAFSPPPPPGRAYDTVRSSFKPGLEAR
                     LGAGAAGLYEPGAALGPLPYPERQKRARSMIILQDSAPESGDAPRPPPAATPPERPKRR
                     PRPPGPDSPYANLGAFSASLFAPSKPQRRKSPLVKQLQVEDAQERAALAVGSPGPGGGS
                     FAREPSPTHRGPRPGGLDYGAGDGPGLAFGGPGPAKDRRLEERRRSTVFLSVGAIEGSA
                     PGADLPSLQPSRSIDERLLGTGPTAGRDLLLPSPVSALKPLVSGPSLGPSGSTFIHPLT
                     GKPLDPSSPLALALAARERALASQAPSRSPTPVHSPDADRPGPLFVDVQARDPERGSLA
                     SPAFSPRSPAWIPVPARREAEKVPREERKSPEDKKSMILSVLDTSLQRPAGLIVVHATS
                     NGQEPSRLGGAEEERPGTPELAPAPMQSAAVAEPLPSPRAQPPGGTPADAGPGQGSSEE
                     EPELVFAVNLPPAQLSSSDEETREELARIGLVPPPEEFANGVLLATPLAGPGPSPTTVP
                     SPASGKPSSEPPPAPESAADSGVEEADTRSSSDPHLETTSTISTVSSMSTLSSESGELT
                     DTHTSFADGHTFLLEKPPVPPKPKLKSPLGKGPVTFRDPLLKQSSDSELMAQQHHAASA
                     GLASAAGPARPRYLFQRRSKLWGDPVESRGLPGPEDDKPTVISELSSRLQQLNKDTRSL
                     GEEPVGGLGSLLDPAKKSPIAAARLFSSLGELSSISAQRSPGGPGGGASYSVRPSGRYP
                     VARRAPSPVKPASLERVEGLGAGAGGAGRPFGLTPPTILKSSSLSIPHEPKEVRFVVRS
                     VSARSRSPSPSPLPSPASGPGPGAPGPRRPFQQKPLQLWSKFDVGDWLESIHLGEHRDR
                     FEDHEIEGAHLPALTKDDFVELGVTRVGHRMNIERALRQLDGS"
     misc_feature    2794..2799
                     /label=SDS
     misc_feature    3699..3704
                     /label=SDS
     misc_feature    3816..3821
                     /label=SDS
     misc_feature    4488..4493
                     /label=SDS
     CDS             4826..4891
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      4895..5326
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    5039..5044
                     /label=SDS
     repeat_region   5369..5509
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    5369..5498
                     /label=AAV2 ITR
     repeat_region   5370..5510
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      5584..6039
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6321..6425
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6426..7286
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7457..8045
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaactatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtcg gacacaagca
     1561 ggtggtggct ctgattcgcc agggtggcaa ccgcctcgtc atgaaggttg tgtctgtgac
     1621 aaggaagcca gaagaggacg gggctcggcg cagagcccca ccgcccccca agagggcccc
     1681 cagcaccaca ctgaccctgc gctccaagtc catgacagct gagctcgagg aacttgcctc
     1741 cattcggaga agaaaagggg agaagctgga cgagatgctg gcagccgccg cagagccaac
     1801 gctgcggcca gacatcgcag acgcagactc cagagccgcc accgtcaaac agaggcccac
     1861 cagtcggagg atcacacccg ccgagattag ctcattgttt gaacgccagg gcctcccagg
     1921 cccagagaag ctgccgggct ccttgcggaa ggggattcca cggaccaagt ctgtagggga
     1981 ggacgagaag ctggcgtccc tgctggaagg gcgcttcccg cggagcacct cgatgcaaga
     2041 cccggtgcgc gagggtcgcg gcatcccgcc cccgccgcag accgcgccgc ctcccccgcc
     2101 cgcgccctac tacttcgact cggggccgcc cccggccttc tcgccgccgc ccccgccggg
     2161 ccgcgcctac gacacggtgc gctccagctt caagcccggc ctggaggcgc gcctgggcgc
     2221 gggcgctgcc ggcctgtacg agccgggcgc ggccctcggc ccgctgccgt atcccgagcg
     2281 gcagaagcgc gcgcgctcca tgatcatcct gcaggactcg gcgcccgagt cgggcgacgc
     2341 ccctcgaccc ccgcccgcgg ccaccccgcc cgagcgaccc aagcgccggc cgcggccgcc
     2401 cggccccgac agcccctacg ccaacctggg cgccttcagc gccagcctct tcgctccgtc
     2461 caagccgcag cgccgcaaga gccccctggt gaagcagctg caggtggagg acgcgcagga
     2521 gcgcgcggcc ctggccgtgg gcagccccgg tcccggcggc ggcagcttcg cccgcgagcc
     2581 ctccccgacc caccgcggtc cgcgcccggg tggcctcgac tacggcgcgg gcgatggccc
     2641 ggggctcgcg ttcggcggcc cgggcccggc caaggaccgg cggctggagg agcggcgccg
     2701 ctccactgtg ttcctgtccg tgggggccat cgagggcagc gcccccggcg cggatctgcc
     2761 atccctacag ccctcccgct ccatcgacga gcgcctcctg gggaccggcc ccaccgccgg
     2821 ccgcgacctg ctgctgccct ccccggtgtc tgccctgaag ccgttggtca gcggcccgag
     2881 cctggggccc tcgggttcca ccttcatcca cccactcacc ggcaaacccc tggaccccag
     2941 ctcacccctg gcccttgccc tggctgcccg agagcgagct ctggcctccc aggcgccctc
     3001 ccggtccccc acacccgtgc acagtcccga cgccgaccgc cccggacccc tgtttgtgga
     3061 tgtacaggcc cgggacccag agcgagggtc cctggcttcc ccggctttct ccccacggag
     3121 cccagcctgg attcctgtgc ctgctcgcag ggaggcagag aaggtccccc gggaggagcg
     3181 gaagtcaccc gaggacaaga agtccatgat cctcagcgtc ctggacacat ccctgcagcg
     3241 gccagctggc ctcatcgttg tgcacgccac cagcaacggg caggagccca gcaggctggg
     3301 gggggccgaa gaggagcgcc cgggcacccc ggagttggcc ccggccccca tgcagtcagc
     3361 ggctgtggca gagcccctgc ccagcccccg ggcccagccc cctggtggca ccccggcaga
     3421 cgccgggcca ggccagggca gctcagagga agagccagag ctggtgtttg ctgtgaacct
     3481 gccacctgcc cagctgtcgt ccagcgatga ggagaccagg gaggagctgg cccgaattgg
     3541 gttggtgcca ccccctgaag agtttgccaa cggggtcctg ctggccaccc cactcgctgg
     3601 cccgggcccc tcgcccacca cggtgcccag cccggcctca gggaagccca gcagtgagcc
     3661 accccctgcc cctgagtctg cagccgactc tggggtggag gaggctgaca cacgcagctc
     3721 cagcgacccc cacctggaga ccacaagcac catctccacg gtgtccagca tgtccacctt
     3781 gagctcggag agcggggaac tcactgacac ccacacctcc ttcgctgacg gacacacttt
     3841 tctactcgag aagccaccag tgcctcccaa gcccaagctc aagtccccgc tggggaaggg
     3901 gccggtgacc ttcagggacc cgctgctgaa gcagtcctcg gacagcgagc tcatggccca
     3961 gcagcaccac gccgcctctg ccgggctggc ctctgccgcc gggcctgccc gccctcgcta
     4021 cctcttccag agaaggtcca agctatgggg ggaccccgtg gagagccggg ggctccctgg
     4081 gcctgaagac gacaaaccaa ctgtgatcag tgagctcagc tcccgcctgc agcagctgaa
     4141 caaggacacg cgttccctgg gggaggaacc agttggtggc ctgggcagcc tgctggaccc
     4201 tgccaagaag tcgcccatcg cagcagctcg gctcttcagc agcctcggtg agctgagctc
     4261 catttcagcg cagcgcagcc ccgggggccc gggcggcggg gcctcgtact cggtgaggcc
     4321 cagtggccgc taccccgtgg cgagacgcgc cccgagcccg gtgaagcccg cgtcgctgga
     4381 gcgggtggag gggctggggg cgggcgcggg gggcgcaggg cggcccttcg gcctcacgcc
     4441 ccccaccatc ctcaagtcgt ccagcctctc catcccgcac gagcccaagg aggtgcgctt
     4501 cgtggtgcgc agcgtgagcg cgcgcagtcg ctccccctcg ccgtcgccgc tgccctcgcc
     4561 cgcgtccggc cccggccccg gcgcccccgg cccacgccga cccttccagc agaagccgct
     4621 gcagctctgg agcaagttcg acgtgggcga ctggctggag agcatccacc taggcgagca
     4681 ccgcgaccgc ttcgaggacc atgagataga aggcgcgcac ctacccgcgc ttaccaagga
     4741 cgacttcgtg gagctgggcg tcacgcgcgt gggccaccgc atgaacatcg agcgcgcgct
     4801 caggcagctg gacggcagct gtacagacta caaagaccat gacggtgatt ataaagatca
     4861 tgacatcgac tacaaggatg acgatgacaa gtaactcgag ataatcaacc tctggattac
     4921 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     4981 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     5041 tccttgtata aatcctggtt agttcttgcc acggcggaac tcatcgccgc ctgccttgcc
     5101 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt tatttgtgaa
     5161 atttgtgatg ctattgcttt atttgtaacc atctagcttt atttgtgaaa tttgtgatgc
     5221 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     5281 tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagat ctggagaccg
     5341 gtgtggcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
     5401 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
     5461 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
     5521 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     5581 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5641 ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5701 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5761 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     5821 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5881 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt
     5941 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat
     6001 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     6061 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     6121 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     6181 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     6241 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     6301 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     6361 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     6421 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     6481 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     6541 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     6601 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     6661 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     6721 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     6781 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     6841 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     6901 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     6961 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     7021 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     7081 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     7141 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     7201 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     7261 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     7321 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     7381 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7441 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7501 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7561 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     7621 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7681 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7741 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7801 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7861 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7921 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7981 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8041 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     8101 acatgt
//