| 质粒名称: | pCgVchcrRNA |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 203815 |
| 存储实验室: | Sheng Yang |
|
载体骨架基本信息 |
|
| 载体名称: | pJYS1Ptac |
| 完整质粒大小: | 6406 bp |
| 载体类型: | Bacterial Expression, CRISPR |
| 载体抗性: | Kanamycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | CRISPR(BsaI) |
| 物种来源: | Vibrio cholera |
| 克隆方法: | Gibson Cloning |
|
文献引用方法 |
|
| 材料和方法部分: | pCgVchcrRNA was a gift from Sheng Yang (Addgene plasmid # 203815 ; http://n2t.net/addgene:203815 ; RRID:Addgene_203815) |
买家导航
pCgVchcrRNA载体质粒基本信息
pCgVchcrRNA质粒图谱载体图谱和pCgVchcrRNA载体序列质粒序列多克隆位点信息
pCgVchcrRNA质粒载体简介
pCgVchcrRNA质粒序列载体序列
LOCUS pCgVchcrRNA 6406 bp ds-DNA circular SYN 07-AUG-2023
DEFINITION Expression of CRISPR RNA, shuttle vector for Corynebacterium
glutamicum / E. coli.
ACCESSION .
VERSION .
KEYWORDS pCgVchcrRNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6406)
TITLE Sheng Yang Plasmids 2023
REFERENCE 2 (bases 1 to 6406)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 7, 2023 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6406
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(100..894)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(211..230)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 821..840
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
rep_origin complement(3853..4591)
/direction=LEFT
/label=CloDF13 ori
/note="Plasmids containing the CloDF13 (CDF) origin of
replication can be propagated in E. coli cells that contain
additional plasmids with compatible origins."
terminator 4819..4846
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 4938..5023
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS complement(5104..5727)
/codon_start=1
/gene="tetR from transposon Tn10"
/product="tetracycline repressor TetR"
/label=TetR
/note="TetR binds to the tetracycline operator tetO to
inhibit transcription. This inhibition can be relieved by
adding tetracycline or doxycycline."
/translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCKSGS"
primer_bind 5635..5654
/label=Tet-R
/note="Tetracycline resistance gene, reverse primer"
protein_bind 6147..6165
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O1 for the tetR and tetA genes"
ORIGIN
1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
61 caaatatgta tccgctcatg aattaattca agcttcatat cagaagaact cgtcaagaag
121 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
181 gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
241 atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
301 caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg
361 catccgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
421 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
481 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc
541 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc
601 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc
661 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttggagttc
721 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
781 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag
841 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa
901 cgatcctcat cctgtctctt gatcagatct tgatcccctg cgccatcaga tccttggcgg
961 caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc
1021 tggcaattcc ggttcgcttg ctgtccataa aaccgcccag tctagctatc gccatgtaag
1081 cccactgcaa gctacctgct ttctctttgc gcttgcgttt tcccttgtcc agatagccca
1141 gtagctgaca ttcatccggg gtcagcaccg tttctgcgga ctggctttct acgtgttccg
1201 cttccggtga ccgcctcgat gatcgccggg tgggcgtggc ccaaggagga tcatccagcc
1261 attcggggtc gttcactggt tcccctttct gatttctggc atagaagaac ccccgtgaac
1321 tgtgtggttc cgggggttgc tgatttttgc gagacttctc gcgcaattcc ctagcttagg
1381 tgaaaacacc atgaaacact agggaaacac ccatgaaaca cccattaggg cagtagggcg
1441 gcttcttcgt ctagggcttg catttgggcg gtgatctggt ctttagcgtg tgaaagtgtg
1501 tcgtaggtgg cgtgctcaat gcactcgaac gtcacgtcat ttaccgggtc acggtgggca
1561 aagagaacta gtgggttaga cattgttttc ctcgttgtcg gtggtggtga gcttttctag
1621 ccgctcggta aacgcggcga tcatgaactc ttggaggttt tcaccgttct gcatgcctgc
1681 gcgcttcatg tcctcacgta gtgccaaagg aacgcgtgcg gtgaccacga cgggcttagc
1741 ctttgcctgc gcttctagtg cttcgatggt ggcttgtgcc tgcgcttgct gcgcctgtag
1801 tgcctgttga gcttcttgta gttgctgttc tagctgtgcc ttggttgcca tgctttaaga
1861 ctctagtagc tttcctgcga tatgtcatgc gcatgcgtag caaacattgt cctgcaactc
1921 attcattatg tgcagtgctc ctgttactag tcgtacatac tcatatttac ctagtctgca
1981 tgcagtgcat gcacatgcag tcatgtcgtg ctaatgtgta aaacatgtac atgcagattg
2041 ctgggggtgc agggggcgga gccaccctgt ccatgcgggg tgtggggctt gccccgccgg
2101 tacagacagt gagcaccggg gcacctagtc gcggataccc cccctaggta tcggacacgt
2161 aaccctccca tgtcgatgca aatctttaac attgagtacg ggtaagctgg cacgcatagc
2221 caagctaggc ggccaccaaa caccactaaa aattaatagt ccctagacaa gacaaacccc
2281 cgtgcgagct accaactcat atgcacgggg gccacataac ccgaaggggt ttcaattgac
2341 aaccatagca ctagctaaga caacgggcac aacacccgca caaactcgca ctgcgcaacc
2401 ccgcacaaca tcgggtctag gtaacactga aatagaagtg aacacctcta aggaaccgca
2461 ggtcaatgag ggttctaagg tcactcgcgc tagggcgtgg cgtaggcaaa acgtcatgta
2521 caagatcacc aatagtaagg ctctggcggg gtgccatagg tggcgcaggg acgaagctgt
2581 tgcggtgtcc tggtcgtcta acggtgcttc gcagtttgag ggtctgcaaa actctcactc
2641 tcgctggggg tcatctctgg ctgaattgga agtcatgggc gaacgccgca ttgagctggc
2701 tattgctact aagaatcact tggcggcggg tggcgcgctc atgatgtttg tgggcactgt
2761 tcgacacaac cgctcacagt catttgcgca ggttgaagcg ggtattaaga ctgcgtactc
2821 ttcgatggtg aaaacatctc agtggaagaa agaacgtgca cggtacgggg tggagcacac
2881 ctatagtgac tatgaggtca cagactcttg ggcgaacggt tggcacttgc accgcaacat
2941 gctgttgttc ttggatcgtc cactgtctga cgatgaactc aaggcgtttg aggattccat
3001 gttttcccgc tggtctgctg gtgtggttaa ggccggtatg gacgcgccac tgcgtgagca
3061 cggggtcaaa cttgatcagg tgtctacctg gggtggagac gctgcgaaaa tggcaaccta
3121 cctcgctaag ggcatgtctc aggaactgac tggctccgct actaaaaccg cgtctaaggg
3181 gtcgtacacg ccgtttcaga tgttggatat gttggccgat caaagcgacg ccggcgagga
3241 tatggacgct gttttggtgg ctcggtggcg tgagtatgag gttggttcta aaaacctgcg
3301 ttcgtcctgg tcacgtgggg ctaagcgtgc tttgggcatt gattacatag acgctgatgt
3361 acgtcgtgaa atggaagaag aactgtacaa gctcgccggt ctggaagcac cggaacgggt
3421 cgaatcaacc cgcgttgctg ttgctttggt gaagcccgat gattggaaac tgattcagtc
3481 tgatttcgcg gttaggcagt acgttctcga ttgcgtggat aaggctaagg acgtggccgc
3541 tgcgcaacgt gtcgctaatg aggtgctggc aagtctgggt gtggattcca ccccgtgcat
3601 gatcgttatg gatgatgtgg acttggacgc ggttctgcct actcatgggg acgctactaa
3661 gcgtgatctg aatgcggcgg tgttcgcggg taatgagcag actattcttc gcacccacta
3721 aaagcggcat aaaccccgtt cgatattttg tgcgatgaat ttatggtcaa tgtcgcgggg
3781 gcaaactatg atgggtcttg ttgtttagct agctcactcg gtcgctacgc tccgggcgtg
3841 agactgcggc gggcgctgcg gacacataca aagttaccca cagattccgt ggataagcag
3901 gggactaaca tgtgaggcaa aacagcaggg ccgcgccggt ggcgtttttc cataggctcc
3961 gccctcctgc cagagttcac ataaacagac gcttttccgg tgcatctgtg ggagccgtga
4021 ggctcaacca tgaatctgac agtacgggcg aaacccgaca ggacttaaag atccccaccg
4081 tttccggcgg gtcgctccct cttgcgctct cctgttccga ccctgccgtt taccggatac
4141 ctgttccgcc tttctccctt acgggaagtg tggcgctttc tcatagctca cacactggta
4201 tctcggctcg gtgtaggtcg ttcgctccaa gctgggctgt aagcaagaac tccccgttca
4261 gcccgactgc tgcgccttat ccggtaactg ttcacttgag tccaacccgg aaaagcacgg
4321 taaaacgcca ctggcagcag ccattggtaa ctgggagttc gcagaggatt tgtttagcta
4381 aacacgcggt tgctcttgaa gtgtgcgcca aagtccggct acactggaag gacagatttg
4441 gttgctgtgc tctgcgaaag ccagttacca cggttaagca gttccccaac tgacttaacc
4501 ttcgatcaaa ccacctcccc aggtggtttt ttcgtttaca gggcaaaaga ttacgcgcag
4561 aaaaaaagga tctcaagaag atcctttgat cttttctact gaaccgctct agatttcagt
4621 gcaatttatc tcttcaaatg tagcacctga agtcagcccc atacgatata agttgtaatt
4681 ctcatgttag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag
4741 ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc
4801 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt aatttgatgc
4861 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cgcaacgttc
4921 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
4981 aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttaaattct agactgatga
5041 atcccctaat gatttttatc aaaatcatta aggttaccat cacggaaaaa ggttatgctg
5101 cttttaagac ccacttttac atttaagttg tttttctaat ccgcatatga tcaattcaag
5161 gccgaataag aaggctggct ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa
5221 taatggcggc atactatcag tagtaggtgt ttccctttct tctttagcga cttgatgctc
5281 ttgatcttcc aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg catataatgc
5341 attctctagt gaaaaacctt gttggcataa aaaggctaat tgattttcga gagtttcata
5401 ctgtttttct gtaggccgtg tacctaaatg tacttttgct ccatcgcgat gacttagtaa
5461 agcacatcta aaacttttag cgttattacg taaaaaatct tgccagcttt ccccttctaa
5521 agggcaaaag tgagtatggt gcctatctaa catctcaatg gctaaggcgt cgagcaaagc
5581 ccgcttattt tttacatgcc aatacaatgt aggctgctct acacctagct tctgggcgag
5641 tttacgggtt gttaaacctt cgattccgac ctcattaagc agctctaatg cgctgttaat
5701 cactttactt ttatctaatc tagacatgtt gtgtctcctc taaagattgt aggaaatgca
5761 atgtgtcaag cagttaggtc aatcagagca tcggtcactc aagaattctt gagctgacag
5821 aacccaaggg cgagtatggc gaatttttcc aactcacgca ccaaacctac tcacattgga
5881 taattcgtcg agtcaactcc gtgtcaacct gtcctcaact ttagcgggga aacaaagatt
5941 tcgcagcaga tgcaaaatca tacagacaca ttcaaaaatg gacattgacc ccaaacgggg
6001 gcatttccaa cacttgcgct aaatagtttt tgcagggaac gaacctgaaa tcgttcgcac
6061 tacgatctgc tcccccgaat ggggttaatt atgaactaca ttacaccccc gtgaatcgaa
6121 ttccaaaaac taaaaaaaat attgacactc tatcattgat agagtataat taacgggatc
6181 cactcttcat tgataagtcg ccaagcttgg gatccccagc ttgttgatac actaatgctt
6241 ttatataggg aaaaggtggt gaactactcc atgggtgaac tgccgagtag gtagctgata
6301 acgagacctc tggtctcgtg aactgccgag taggtagctg ataacggatc cgaattcgag
6361 ctcggcgcgc ctgcaggtcg acaagcttgc gctcgagcgg gggccc
//
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