pCAMBIA1301-proMpEF1a-MpTCP2-GFP

LOCUS       pCAMBIA1301-proMpEF1a-MpTCP2-GFP           16069 bp DNA     circular SYN 03-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 25, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..16069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      39..291
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    313..337
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1637..2266
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2695..3768
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3834..4028
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    4372..4512
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4698..5286)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5373..6167)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6592..6616
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    6694..6868
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6908..7933)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(8001..8678)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    8869..8890
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8905..8935
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8943..8959
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8967..8983
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8999..10727
                     /label=proMpEF1alpha
     gene            10743..15332
                     /label=Mp1g19590
     CDS             15345..16064
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
ORIGIN
        1 cgaatttccc cgatcgggtg accagctcga atttccccga tcgttcaaac atttggcaat
       61 aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt
      121 tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg
      181 tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc
      241 gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattaa
      301 actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta
      361 gaataacgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca
      421 tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat
      481 agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag
      541 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt
      601 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag
      661 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc
      721 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc
      781 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac
      841 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt
      901 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac
      961 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag
     1021 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg
     1081 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc
     1141 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc
     1201 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt
     1261 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc
     1321 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg
     1381 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct
     1441 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
     1501 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     1561 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
     1621 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     1681 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     1741 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     1801 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     1861 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     1921 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     1981 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     2041 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     2101 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     2161 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     2221 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     2281 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     2341 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     2401 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     2461 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
     2521 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     2581 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
     2641 ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc
     2701 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
     2761 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
     2821 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
     2881 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
     2941 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
     3001 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
     3061 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
     3121 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
     3181 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
     3241 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
     3301 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
     3361 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
     3421 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
     3481 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
     3541 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
     3601 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
     3661 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
     3721 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
     3781 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat
     3841 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac
     3901 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
     3961 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc
     4021 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg
     4081 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca
     4141 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca
     4201 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga
     4261 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     4321 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     4381 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
     4441 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     4501 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     4561 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     4621 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4681 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4741 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4801 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4861 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4921 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4981 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     5041 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     5101 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     5161 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     5221 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     5281 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     5341 cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag taaaatataa
     5401 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
     5461 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
     5521 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
     5581 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
     5641 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
     5701 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
     5761 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
     5821 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
     5881 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
     5941 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
     6001 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
     6061 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
     6121 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
     6181 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
     6241 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
     6301 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac
     6361 aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg
     6421 tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt
     6481 ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc
     6541 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat
     6601 atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt
     6661 aatgtactga attaacgccg aattaattcg ggggatctgg attttagtac tggattttgg
     6721 ttttaggaat tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg
     6781 gtttcttata tgctcaacac atgagcgaaa ccctatagga accctaattc ccttatctgg
     6841 gaactactca cacattatta tggagaaact cgagcttgtc gatcgacaga tccggtcggc
     6901 atctactcta tttctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga
     6961 gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc
     7021 cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat
     7081 catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat
     7141 acgcccggag tcgtggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct
     7201 gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg
     7261 aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca
     7321 ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg
     7381 cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca
     7441 cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag
     7501 tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg
     7561 ccgcagcgat cgcatccata gcctccgcga ccggttgtag aacagcgggc agttcggttt
     7621 caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct
     7681 cgctaaactc cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc
     7741 gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc
     7801 cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca
     7861 ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt
     7921 caggcttttt catatctcat tgccccccgg gatctgcgaa agctcgagag agatagattt
     7981 gtagagagag actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata
     8041 gaggaaggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat
     8101 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
     8161 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct
     8221 ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga
     8281 tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt
     8341 gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga
     8401 cgagagtgtc gtgctccacc atgttatcac atcaatccac ttgctttgaa gacgtggttg
     8461 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
     8521 cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc
     8581 caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga
     8641 ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga
     8701 ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttggcaagct
     8761 gctctagcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     8821 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     8881 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     8941 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgaattcca
     9001 aatgagtcac acacattgtt gagagacata tcaaaataac tcaattgtaa ggtaagttga
     9061 tagatttccc agttaaatgc attattttaa caatatttgt atctcaaatt ttcaaaatca
     9121 tatcaaattt gcagatcttg atgatgattc acaactctgt cttcgatttt tgattgttaa
     9181 cgggtatcga tatttgggta acttaaaaag aatttgctaa agatcttaaa aaacagatta
     9241 aaaaaacctt tcaaaatagc taatccttta aatccgtttc acaatataat gctcattgat
     9301 gataatcgaa aagattccat tttaaagtgc atataaattg cttaattatc tctttctgtc
     9361 tcgtaatatt ttgttctctc ctattggatc ggacccgcat cttctcgatc agttcactat
     9421 actaatattt ggtcaacttc aagagagatt agaacttcgt tctccaaagt gtaaaggccg
     9481 acaagcagaa aaggaaactt caatcagaag ccttctaggt tttcccgcgt cccaaaaagc
     9541 gaagggagga cggggaggac ggtgaggcca gaagaagaaa gcaacagtaa aatctcatgt
     9601 gattgcgtgc atgtcatgga tctatgggat gctagtccac cgtttggaag tatgtgcaga
     9661 aggagaaagg cgcgggaggc caaaagcgac ttcggccacc tctctacttt ctagaaagtt
     9721 tgctcgcagc ttgtaataag cgcggaaaag gtactcgaaa aaccggagca atcggtgtgg
     9781 tatcagcagt cgggttcgct aaacctacgt cttcttattt tggaatgtta aattgaaagg
     9841 gcgtgccggc cctaagcacg gacgatgtga cagacggagg agtgtgagca attcggatga
     9901 cgatgctggc cggcagtcac gacaaagagt ccgaaggggc atggacaggg caatatataa
     9961 atggaaaaga ggctttgaat attcctaacc cttcgtcgtc ggcctcccaa cttgccctcg
    10021 catgctgaat tggccgccta tctgcgaatg gccggccgcc gaccctaagt cgcaataact
    10081 tgaacggagc cgatgcaaag tccttatcga acgctcgtta ttataaacgg ggcagaccta
    10141 ggtatttaat tatatcaaaa ccctccgcaa tttcgctaat cattccctaa tttcttctac
    10201 tttggcgtct agtgctgtgg tcgcgcttgc tctcaaagct cgctgggagg cagtggagcg
    10261 tctggcttaa aatcgatcgc cgcaacccta gagttccttt cacttctttc tctcttgcgt
    10321 ggagagcatc gacatcgaag agctgcatct tgtcttctgg tatgcgcctt gtgccctcgt
    10381 ctctgtacgt cgttacgatt gctcgatatt gtgattgtct agacctgaaa cccgactgga
    10441 gcttttacgt ggtgtcgatt ggggcctcga gcgagtgatt ttttaggttc gttttagcca
    10501 agtacatccc actttggtca gtcctgttat ctcggtaagg tgttcagttc ggtctctggt
    10561 gcggaggtgc ctcgtaagtt tcctgcatga tagatttgtc tatccttgac ttgaagagga
    10621 gtcgaaggtc agcgaggttg gctgtgtgct atggacgtcg gtgctccttg gttccgtgct
    10681 tctatgggtg ttgcctcaac agtattgatg tgcctgcaga aaggttgggt accgaaaagc
    10741 ttatgatttt gtctcgcctt cgagagggag ttcaaaactc cggctggact tcgcggcttc
    10801 ggtatgttga tcgagaaatc tcacgagaaa tctcaccatt cttatgcgtt gtttgatcga
    10861 ttttgaccgg gttgttgccg gagcctgtcg aggagaaagg ccgtcgagac ggccgttgtt
    10921 gcaacttctg ggaaaggatc cggaatgtta gcatgggctc ggtctgttga ttcctccacg
    10981 agctgccatg caaggtgggt tatcgatgtc agacggaaac tcaacgtaac tgacccccac
    11041 tgttggttgg cctggtctgg tcttccccat agtatgcacg agagatcgat caggagagag
    11101 tacgcgtcca gtatagatgg acactacagt agatgccttc acacagccgt ctgggatagg
    11161 cttcctgcaa tctaagatga gggtttgacg gacaacttac atactatatc gagtttttcc
    11221 aattgaatgt gtgaaattgt ggacttacac acatggtaag cggtgaacgg atgccaagtg
    11281 gcaatactca ttatcatagc gttcttcaga catatactgc tagatttcgc tcctctcgac
    11341 ttgctttctg cagccgtaga gggctgcagt attgagttct gatgaatgga agttcacttc
    11401 atctaccaca gactgtggaa cttgaaacaa tttttggggt tcttgtccac cgtttcccat
    11461 agttcgaact tctactgtta catgtgaggc gctgtctacg ctgttaaaaa gttccgggtt
    11521 cctgcacttc ctactcccat gggttcgagc atttacgagt agatggtaga ccgtctctta
    11581 ctccttggga aagcctttaa gttcctcctg agaaactgag cataactcgt agagcgcgag
    11641 atgagtgtac gagtgatctt cttctgggcg gaaatcgaat gctatttaaa taggagaagc
    11701 gtctcacctg atcgcagagt gcgagcacct cgttactctt atatgataga gtaacgatgt
    11761 tcatgttgag tgtacagctg aatgtgtaag cttggtcacc tggtcgtagt atgtcctctt
    11821 ctcgcagttt tgtttctagt cacgcacaaa tgtcgagtcg atggtaagca tagagactga
    11881 gaggaatgga gtgagtaaat ttatgtcaca ctgatatact ctatcatata ttctctacac
    11941 gtcacaggtc aacgagctgg aggattgggt tcgagggcaa ctgttggcca gtggaaagag
    12001 atctccaaga cgtttgcaat agacaatgct cggactccac gggatatttg gaagtgtcag
    12061 tcagagctgc gaagcaaccg caatcggagt tgtgcttttc tgtagcgaat ggaagcaagt
    12121 ttgaatcaaa agttgatgag agagcaagag atggagagag gtttcgaata gaaagagatc
    12181 agatcagaca atacgcgcag tccatctaca gaacacttgc agatccacag gcggcggcag
    12241 tcccaacaga acagcggtct tcaggcctgc ggagttcgat cccaggaggc caactcgcat
    12301 gtgatgtaag caaatataga gttcagacag catcggcaaa tcagctgcat cccaattcta
    12361 acgacagttt cgagagcata aatttttccc cttcatcacc catcactgat catcagagag
    12421 agttacgacg tactgaaatt gaggcgggat tggagccaga aggtacgagg agacaaaacg
    12481 ttggagagga agcttcagac caaataaatc gagctacttc tctgaagaac gggtttccac
    12541 tggctgagag agcaagtgct cctgagaagt tcatcgcgga agggctggat gattctgagc
    12601 cagcaagaca cggcagagga ggtcctcaga acaagcggtc tagtatttgg gcaaaaaatg
    12661 tggaactagt gggacatggt cctttcgcct tccgtgagaa cattgtggaa tgcagtacac
    12721 atattggtgt gagtgagaag gaaggcttcg ttttacataa tgtaatcgat gcacaggcct
    12781 cgcatggaag agctaaagag cccacagctg actcagggac accgagaagc caaaattctc
    12841 atcagaggac aatacatccg caaggacgtc aagatagagc caagactgcc aatgccatcc
    12901 ccagtagtcc acaatcgcag gctttgactg atagagtttc tagaggagca gcctctgatg
    12961 cgctaggcac cgatttactt cctcaaagca ttgggagtga tctacttcag cagggaacca
    13021 ccccagcacg agtcgaacga gtccaaaata tatcacggac gagaagtggc ggtgagagag
    13081 acgtggagga taccttaaaa ggccgtagat atccggagag aattggagac cttagagcaa
    13141 ctacagtagc cacagctgga cgccaacata aagcagaaag atctggggaa atacctcctg
    13201 ttcgaacaag tcaagagaca gttgtaggta cagcaggagt tctcacaatt ccagaaagaa
    13261 tcggtgggac ggcagaagat ctgacagcac gcgaaatagt tgatgtttca gaaagccatt
    13321 cagtcagtga aagagttgga ggtagagcag aaacacattc agctcaagac ggtggaagtg
    13381 ccgaaggttt cacgattcga gaaagaactg gagggagtcc atccgaaggc aacaaaattc
    13441 cagaaagaat tggagcagtt tcagacagag actcggcggg gaaggtccaa gagaaaggaa
    13501 aagaagaaaa gctccatccg agggaagagg gacatcaacg gcaaagcgag tcatcagacg
    13561 aaaggaggct tcgggcacga atcgacggct caggtggtag cgcctcagga ggaggtgatg
    13621 agattgagga gcccaaagtc gtgcgtcctg cacggccatc gggtgggaag gatcgccaca
    13681 gcaaagtgaa cactgctaag ggtcccagag atcggagagt gagactatcc gtccctacag
    13741 cagtgcagtt ttacgatgtc caagaccgac ttggctttga tcaaccaagc aaggccgtgg
    13801 aatggcttat tcataaagcc aaggacgcaa tcgatgagct tggtcgtatt cctgtcgatg
    13861 gcaaggatgg acctcgcgta cccagtgtgt gtacgtccga tattacacgt cacgtttcga
    13921 tttcctcttc ttacggtgcg ggcgccgtct caggtcttgt gagtggcact tcatcttgtt
    13981 cacctgcaag ttcattccaa ggcccttcca gtatgtcgta tggagatcag ctgagcttct
    14041 taatgaaccc atctattgct cagggaatgt ttgcgcctct acagccaaat caaggaaatt
    14101 ttgcaggtcc aacctttggt gtaggaagaa tggatctgac taggccgaat agcggatctg
    14161 cggaaggagg cagatcgaat gaatcagcag gatctccatt ggcacgtgtg gaatccagga
    14221 caaaggcgag agagagggca agggagagga cgaaaaaggg tcccggaaga gatggaaacc
    14281 aagggaaagt caccagcggt ggttcgccgt catcctctca gtcacctcct ttccatccta
    14341 atcccgcatt taactccttc aatgcgtcaa attccattca gagttctggt catggcttgt
    14401 ccttacagag cccacatcaa agcgcgctac tccccgccgc atatgacgga agcccattct
    14461 cgaggatcat gcaaggttct caccaagcca cctttcaaaa tatgatcatg cacaatcccc
    14521 ctctggactg tcagtttggc gatcagactc aaagccctct tcacaatgcg tttctggtcc
    14581 agcctcaagc ttctttacca agtccttctc acactatgaa cacgttacag cacccacatt
    14641 tgcagacgat gcaagtaaac gctcaaggag cccttcactt gggtaccaat ttcaatcccg
    14701 gaccatgttc atcttccttc gccgaggcat ctatgtcctc tcctcattat ccacccgttc
    14761 ccactcttcc gatatctcaa tcgctctcga ttcctcagtt gtcttcccta ggtatgtctc
    14821 ctgccagttc gctaggacct cattacccca ccggaagccc gacaccgata cctccaggat
    14881 tttctcccgg tcctcctgct tcagcatttc gagcccattc cttgtccata agccaatgct
    14941 caaatccgaa tcagaatcat cagcagcagc agcaacagct tcaaggattg tcaagagttc
    15001 aacgtccagt tccgtccttc agcactactc catcgacgtc gcatgccgga tcttcttcgt
    15061 caagggcaca tgcacacccg tactactcag ggaattcttc gacggcactc atgggctgtc
    15121 ttcaatccat gccatcgttc gcacccagaa gcaatgtggg ctacgagcat ggacaacgag
    15181 tgcgtcctat gatgtctcct aatcgaattc attcctatca agcatccaac tctcagatac
    15241 cggcgagaat tcatggtctc gatgacatgg aagagctcga ggaagagccg aatccagcaa
    15301 caccatcttc tccacatctt tctccccacc ctggatccgg taccatggtg agcaagggcg
    15361 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
    15421 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
    15481 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
    15541 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
    15601 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
    15661 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
    15721 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
    15781 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
    15841 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
    15901 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
    15961 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
    16021 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaagagct
//