| 质粒名称: | pQL261 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002536 |
| 产品用途: | Lentivirus vector for PhCMVmin-driven mCherry expression (ITR-PhCMVmin-mCherry-pA-ITR). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pLenti-CMV |
| 表达系统: | 慢病毒表达 |
| 启动子: | CMVMIN |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | mCherry |
| 物种来源: | Discosoma |
| 密码子优化: | 未优化 |
pQL261
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pQL261载体质粒基本信息
pQL261质粒图谱载体图谱和pQL261载体序列质粒序列多克隆位点信息
pQL261质粒载体简介
pQL261质粒序列载体序列
LOCUS pQL261 7158 bp DNA circular SYN 08-APR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM recombinant plasmid
REFERENCE 1 (bases 1 to 7158)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 29, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7158
/mol_type="other DNA"
/organism="recombinant plasmid"
primer_bind 212..232
/label=QL426-F
enhancer 238..617
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 619..817
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
LTR 835..1015
/label=HIV-1 5 LTR
misc_feature 1062..1187
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1126..1170
/label=HIV-1 psi pack
misc_feature 1222..1227
/label=U6 terminator
misc_feature 1251..1256
/label=U6 terminator
misc_feature 1665..1670
/label=U6 terminator
misc_feature 1684..1917
/label=RRE
misc_feature 2444..2561
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2620..2932
/label=U6 promoter
/note="RNA polymerase III promoter for mouse U6 snRNA (Das
et al., 1988)"
misc_feature 2845..2850
/label=U6 terminator
primer_bind 2949..2965
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2994..3008)
/label=QL144-R
misc_recomb 3007..3040
/label=LoxP
protein_bind 3007..3040
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
primer_bind 3048..3088
/label=引物 1
primer_bind 3048..3068
/label=QL426-F
primer_bind 3067..3085
/label=Oscl23-1
misc_feature 3069..3216
/label=hcmvmin
misc_feature 3138..3152
/label=New Feature
primer_bind complement(3207..3227)
/label=ql228 98-R
primer_bind 3208..3247
/label=QL 431-F
CDS 3228
/codon_start=1
/product="start codon"
/label=ATG
/translation=""
CDS 3231..3935
/codon_start=1
/label=mCherry
/translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
GRHSTGGMDELYK"
primer_bind complement(3915..3939)
/label=QL138-R
primer_bind complement(3918..3959)
/label=QL144-R
primer_bind complement(3918..3958)
/label=引物 2
primer_bind complement(3919..3938)
/label=QL 430-R
misc_recomb 3958..3991
/label=LoxP
protein_bind 3958..3991
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
misc_feature 4047..4635
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4409..4427)
/label=oPLL3.7-5
CDS complement(4518..4529)
/label=FactorXa site
primer_bind complement(4638..4654)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
LTR 5164..5344
/label=HIV-1 5 LTR
primer_bind complement(5361..5380)
/label=oYW207
/note="pLL3.7 PCR R"
rep_origin complement(5388..6016)
/direction=LEFT
/label=ColE1 origin
rep_origin complement(5406..5994)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5545..5564)
/label=W5CF0703-P099-D09
/note="Sequencing primer"
misc_feature 5946..5951
/label=U6 terminator
misc_feature 5947..5952
/label=U6 terminator
misc_feature 5979..5984
/label=U6 terminator
misc_feature 5980..5985
/label=U6 terminator
primer_bind 6009..6028
/label=oPLL3.7-8
CDS complement(6165..7025)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
CDS complement(6168..6827)
/label=AmpR
primer_bind complement(6182..6201)
/label=W4CF0701-P099-E08
/note="Sequencing primer"
misc_feature 6577..6582
/label=U6 terminator
primer_bind complement(6846..6865)
/label=W3CF0630-P099-D08
/note="Sequencing primer"
primer_bind complement(6902..6921)
/label=W3CF0630-P099-A08
/note="Sequencing primer"
primer_bind complement(6902..6921)
/label=W3CF0630-P099-F07
/note="Sequencing primer"
misc_feature 6984..6989
/label=U6 terminator
primer_bind 6996..7015
/label=oYW24
/note="Forward seq primer of AmpR"
promoter complement(7026..7130)
/gene="bla"
/label=AmpR promoter
promoter complement(7067..7095)
/label=Amp prom
primer_bind complement(7070..7093)
/label=oPLL3.7-7
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
2581 gcagagatcc agtttggtta gtaccgggcc cgctctagag atccgacgcg ccatctctag
2641 gcccgcgccg gccccctcgc acagacttgt gggagaagct cggctactcc cctgccccgg
2701 ttaatttgca tataatattt cctagtaact atagaggctt aatgtgcgat aaaagacaga
2761 taatctgttc tttttaatac tagctacatt ttacatgata ggcttggatt tctataagag
2821 atacaaatac taaattatta ttttaaaaaa cagcacaaaa ggaaactcac cctaactgta
2881 aagtaattgt gtgttttgag actataaata tcccttggag aaaagccttg ttaacgcgcg
2941 gtgaccctcg aggtcgacgg tatcgataag ctcgcttcac gagattccag caggtcgagg
3001 gacctaataa cttcgtatag catacattat acgaagttat attaagggtt ccaagcttaa
3061 gcggccgctc gagctcggta cccgggtcga gtaggcgtgt acggtgggag gcctatataa
3121 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
3181 tccatagaag acaccgggac cgatccagcc tccgcggccc cgaattcatg gtgagcaagg
3241 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
3301 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
3361 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
3421 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
3481 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
3541 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
3601 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
3661 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
3721 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
3781 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
3841 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
3901 gccactccac cggcggcatg gacgagctgt acaagtaaga attcgtcgag ggacctaata
3961 acttcgtata gcatacatta tacgaagtta tacatgttta agggttccgg ttccactagg
4021 tacaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
4081 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
4141 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
4201 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
4261 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
4321 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
4381 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
4441 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
4501 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
4561 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
4621 gggccgcctc cccgcatcga taccgtcgac ctcgatcgag acctagaaaa acatggagca
4681 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
4741 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
4801 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
4861 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
4921 gattggcaga actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc
4981 tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc
5041 cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag
5101 tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt
5161 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
5221 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
5281 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
5341 agcagcatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
5401 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
5461 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
5521 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
5581 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
5641 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
5701 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
5761 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
5821 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
5881 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
5941 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
6001 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
6061 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
6121 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
6181 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
6241 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
6301 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
6361 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
6421 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
6481 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
6541 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
6601 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
6661 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
6721 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
6781 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
6841 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
6901 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
6961 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
7021 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
7081 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
7141 cgaaaagtgc cacctgac
//
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