pCAMBIA1301-MpCDF-GFP

LOCUS       pCAMBIA1301-MpCDF-GFP          15110 bp DNA     circular SYN 03-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 24, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..15110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      39..291
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    313..337
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1637..2266
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2695..3768
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3834..4028
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    4372..4512
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4698..5286)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5373..6167)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6592..6616
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    6694..6868
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6908..7933)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(8001..8678)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    8869..8890
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8905..8935
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8943..8959
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8967..8983
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            8999..14373
                     /label=Mp1g18540 promoter+gene
     CDS             14386..15105
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
ORIGIN
        1 cgaatttccc cgatcgggtg accagctcga atttccccga tcgttcaaac atttggcaat
       61 aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt
      121 tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg
      181 tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc
      241 gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattaa
      301 actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta
      361 gaataacgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca
      421 tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat
      481 agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag
      541 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt
      601 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag
      661 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc
      721 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc
      781 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac
      841 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt
      901 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac
      961 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag
     1021 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg
     1081 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc
     1141 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc
     1201 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt
     1261 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc
     1321 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg
     1381 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct
     1441 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
     1501 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     1561 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
     1621 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     1681 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     1741 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     1801 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     1861 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     1921 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     1981 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     2041 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     2101 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     2161 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     2221 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     2281 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     2341 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     2401 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     2461 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
     2521 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     2581 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
     2641 ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc
     2701 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
     2761 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
     2821 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
     2881 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
     2941 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
     3001 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
     3061 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
     3121 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
     3181 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
     3241 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
     3301 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
     3361 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
     3421 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
     3481 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
     3541 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
     3601 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
     3661 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
     3721 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
     3781 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat
     3841 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac
     3901 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
     3961 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc
     4021 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg
     4081 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca
     4141 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca
     4201 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga
     4261 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     4321 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     4381 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
     4441 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     4501 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     4561 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     4621 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4681 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4741 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4801 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4861 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4921 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4981 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     5041 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     5101 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     5161 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     5221 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     5281 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     5341 cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag taaaatataa
     5401 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
     5461 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
     5521 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
     5581 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
     5641 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
     5701 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
     5761 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
     5821 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
     5881 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
     5941 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
     6001 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
     6061 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
     6121 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
     6181 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
     6241 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
     6301 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac
     6361 aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg
     6421 tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt
     6481 ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc
     6541 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat
     6601 atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt
     6661 aatgtactga attaacgccg aattaattcg ggggatctgg attttagtac tggattttgg
     6721 ttttaggaat tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg
     6781 gtttcttata tgctcaacac atgagcgaaa ccctatagga accctaattc ccttatctgg
     6841 gaactactca cacattatta tggagaaact cgagcttgtc gatcgacaga tccggtcggc
     6901 atctactcta tttctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga
     6961 gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc
     7021 cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat
     7081 catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat
     7141 acgcccggag tcgtggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct
     7201 gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg
     7261 aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca
     7321 ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg
     7381 cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca
     7441 cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag
     7501 tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg
     7561 ccgcagcgat cgcatccata gcctccgcga ccggttgtag aacagcgggc agttcggttt
     7621 caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct
     7681 cgctaaactc cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc
     7741 gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc
     7801 cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca
     7861 ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt
     7921 caggcttttt catatctcat tgccccccgg gatctgcgaa agctcgagag agatagattt
     7981 gtagagagag actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata
     8041 gaggaaggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat
     8101 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
     8161 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct
     8221 ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga
     8281 tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt
     8341 gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga
     8401 cgagagtgtc gtgctccacc atgttatcac atcaatccac ttgctttgaa gacgtggttg
     8461 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
     8521 cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc
     8581 caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga
     8641 ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga
     8701 ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttggcaagct
     8761 gctctagcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     8821 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     8881 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     8941 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgaattcac
     9001 ccgctcgttg gaagcgaggg gatggggcaa tcttactttc ctctacgaag ctttcccagc
     9061 gacaagagcg gcgaggaaga aaggcgatta tgaagatcag acatccatta aagaaagggt
     9121 gggctccaca cgagtcgcga gtttcccttc tgcgacgagt cgtgaaaata accgaataca
     9181 acctggccgc aattggtaga ttttcgatgc tcgttacgcc gacggggagc gacaagtatg
     9241 cctgcgcagg attatcgtta aaagcgtcgg tgtcacgtca ccatgtgctc gaaggaatga
     9301 agaagaagaa gaagaagaag ccgaaagaag gaaaatccca agggttgttt cctctaagct
     9361 tcctggacct ggtgagcgat gtggcaccgg tcgagtcgac aaagaatgcc aaatagccgg
     9421 actgtgaccc ggatcggctg gagaaacact caaatgtggc cgcccttgtc atgtcccgca
     9481 accactatgg gctacggaga gcatcttttg gaagaacgag agaattggcg cttcgaaggc
     9541 gagagagcgc gcgagcgagc tagctccgtt gcatctctcg gactctaaat cagagagagc
     9601 acgagatgca cgcctccgca aagacaatat catactcgtc gctcccgatg atgccccagc
     9661 tatgacatga cctcatcgcg ctcgctcaac tcctcaagaa gcaccggcga ggagaccatc
     9721 ctttttgctg tattacaggg ggccaccttt tttttctgga ggcgggcgcc gaagccttat
     9781 catttgcttt gctttccttt tatttttccc cggggggcct tccctttccc ggaaaagcaa
     9841 tgaaagaaag aaagaaaaga gatatgattt accagcgaaa gaggtctttt tggccagaaa
     9901 aggtcgacgc gtcggcccca tgattcatgc gataagcagc gcatcgacgt gctgcgggac
     9961 cggctcgtcg gggaaaacag ggtcggggct gcagcttcgg ggatagaaga taccagaagt
    10021 tcccagcccg gcccgcccgt tcggcggcgg ccggcctggg aagggagacc ttggagcgcg
    10081 gcagcctcga ccctgacggt gacccccggt ccacatcatc cgagcggagg agccagctca
    10141 aatgatgatg aggagtttca cctggcgagg agtcgagaac ggtggccacg ttggatcggg
    10201 aaaggaagaa tatacggaac tgtattttca cgtgaactaa tacgggcatg cgtacgtggc
    10261 gcccgaagag gaaaagattt tcatgcctcg gtgctgagcc acacgttatg agtggctctc
    10321 gttcgctccc acgtgagcac tcgcgacgct tttgagttcg agcaattctt gtaactagtg
    10381 gatggcctgg ccctaaagca aagcggagca aaagctctca agtggaaccc tccgctgctc
    10441 gaaagcgacc tgctgcttat cgcgagcgtc tccatgtccg ccagccggtc gtcgaccggc
    10501 tgccggagcg cccaggagcg gtttagggct cgcttttcgc ccctgctcct tgcagagcat
    10561 ggcggttttt tattcgcaca gcttttgccg atcttaccca cacctcacac accggcctcc
    10621 ctcgtcctcg gccatcgagc agccacgggc ccgggccggg acgccgaccg gcgcgaactc
    10681 caggcgggcc aggtcgcccc gtaggccatg tcggggaaga cgggcggaag cagggtcgag
    10741 gccgaccagc cggtgccgcc accggcgagc gaccggttcg cgcgaaaacc ccccaggccc
    10801 agcagtgcag aggagccacc gcatttcctt acacagccag cgctggttct cacctcctca
    10861 caactctccc cgtgttcgcc ctctcggccg tcgtcgtctc ttctccacct ctcctttccc
    10921 cggtcccccg cgcggtcctt gatcgatcga tcgatccatc gcgcgcccgc ctgccttccc
    10981 tctctcctcc ctttcctccc ttccttgatg agagtgaagc tcttcgtctc gtgtgcctct
    11041 cgtctccgct gcctgcttgt cggactggct ggcgtgcttg gctgggctgg cctggtgtct
    11101 gcggccgacc atcaacgacg acgtctcgac atgaggctct cttgaagcgc cgagagctgg
    11161 tttcgctgtg agcttgtggc tgtgtgtgcg tgtgtgtgag cgagcgagtg tgtgattgac
    11221 tcttgtgcgt ggcagcgctc tctgtcgaca ggtcgaggcc gagcgcagtt cgcttctact
    11281 attcacctcc tgtgctcttt catgggcgga ggagcgagcg aagttgcgag cgtgacgggc
    11341 gaagaagaag cgctaggaag cagggacaga ctttgccatc aacgagtgct gtgtgtgcga
    11401 taggaaggga agggccgatt tcggtaggta atgtgcttcg cgagtaagct ggatctggtg
    11461 gattggagga ggcgcccgct ggtagacgat aggtaattga cagacagaca gacagacagg
    11521 ttgcaatctt gacaggtccg ttgctgtgga gagtccgtgg gaaggtgccg gtgggtggtg
    11581 gtaataaggg gcgtggaggg caaggtggta tggaagaagt gttctggtca ggaaacaggg
    11641 tgaagagaaa ggacgatata tcagcgatct gttgaagaag ttctagctgt agaaaaagaa
    11701 aggccgagtt tagggagcgc gattccctga ggaatagttt tctgggtaaa gatctgcgac
    11761 ggaggctgaa ggctgggtca ggacgtggga cggcacgaaa tggcatgtgc ttgaggatat
    11821 ggattgccgt cgaagctcaa gaatttcagt atgatggatt caccgatcaa gttgttcggg
    11881 aaaacaattg ctaaagctgc cgcttccgca ttcacaacat catcaggtga agcagacgac
    11941 gctgaagcag tcccgactac gagcagtggg ggctgggttg atttgccatc gtcgtcgcag
    12001 gtgtgtactt cccttccagc tctttgaatg tatgcaagct gatcttgcct acctgacgag
    12061 cattcgagca gcccttcgtc gctcgtcaca gatgcgtttc cgtgtctttt gcatagccac
    12121 tcctcaggag ggctcaggct ccgcgcctgc gtgtttcaat cgatgcctgc gtgagtgttt
    12181 tgtgcgcttt atgaattggt tcaatcgccg tataggatag gatagtatag gatgcgatat
    12241 gctttctttc catggagggg tggctcgccg agcacgtcgg cagccggtgg cgaagtcgct
    12301 agtccgtctc aatgtcgcct gcgccagagc agagcatcgc gggccgtcgg gactgacgtg
    12361 ggcgggcgtg caatttctgt ctgggatgtg caggatgtga aggaaaatgg agcggaaagc
    12421 agcagactgg gggagatcaa tggaggcggg gtgacgttca cgaggcagga ggtgaaacga
    12481 ggatcgtcgc ccgaccggac gtcgtcggcg tgcaacagcg cggaggagga gaagctgtgc
    12541 gcgtcgccgc gaggcggagg cggggccacg gggggagcag gagcagagct gacgccctcg
    12601 gcctcggggg gcgaggcggg cgcgggcgac gacgagggca gcgaggagga ggatggatcg
    12661 ggcatgaatg ggcgcagtaa aaaggatggc ggcgacgatg gcggcaaggg aggggagaag
    12721 atgccccgac ggcccgagaa tgctgttgcg tgtcctcgat gccatagttt tgagacgaag
    12781 ttttgttact acaacaatta caacgtcaat cagccccggc acttctgcaa gcactgccag
    12841 cgctactgga ccgcgggcgg cacgctgcgc aacgtgccgg tgggcgccgg gcggcgcaag
    12901 aacaagcacg gcagcaccat cgcgccgcgc ggccagacgc ccgaggtcgg caccgtgcgc
    12961 agcgacgcgc ccgacatcgc ctcgccgctc atcgcgtcca agggcggcgt cgggcccgtg
    13021 cccgtgcagg tcgcggtcgt gcaggccgcg ggcagcaagg gcgggattgt gcgcatggcc
    13081 gtgcctgcgg ccagcggcga cccctcggtt ctgccgtttc tgcgaatgca gccctcgccg
    13141 atggacaccg cctccatctt catggcctcg tccgatgccg gcacctcgag cgacacggtc
    13201 accgtggcct gcaagcgcgg ccggctggcc actcccgggt tcagcgacgc atccacaggt
    13261 gccaacgaca tggagcagca gcagcagcag cagcagcagc cgtcttgggg gcagcagtcg
    13321 tcgacgaatg atgaatccgc ctgctcgccc ctcacgtcgg cgaatggcaa tgttgtggtg
    13381 catgagatta agggcgaacc cgggtcagcg tgtcagatga tgggtgtggc agcggtatca
    13441 ggagtggggg ggcccgagca gcagcagcag cagcagcaac aacagcaaca gcagcagcag
    13501 cagcagcagc atcctgcgac gaacaacggc atggccggtc cagggggttg ttggtctgct
    13561 gccggtccgc aatcgagcgt acctccgccc ggggccgcgg gagcccctta cggattctat
    13621 aacggaggat ggccgtatgg gtacaacgtg ggctggagcg ggcctcctgc attctgcgcc
    13681 gggggagttg ccactgcggg cacgggcatg ccgggccctc ccccgccggg caacatcgct
    13741 gcgtggaatg gagccggggg cagcgtctgg accggggttc cttggcccat gccgggcctg
    13801 gcttggggcc ccccctgggc tatggcgccg tgggccatga gtgcgggtca cggtggggct
    13861 agcatgggga gcattacgca gacgatttcg atgagctccg gtgctgctgc tggcatggtc
    13921 actgtgccgg tgccctgcag tacgactgcc agtggtggtg gtcctgcgtc cgctgcgccc
    13981 ccgtcatcgc cctcatcatc actgggcaag cattcgcgcg gggggccgcc ggtgggggcc
    14041 cctgcatcgt ctgccgcagc agctgctgct tgcaatggcg caggaggagc aggtggggga
    14101 ggaggaggcg gcggaggagg ccgcatggag ggaggcctgt gggcgcccaa atcgctgcga
    14161 atggctgatc ctgaggaggc aatgcgaagt tcggtttgga ggatactggg agtcggttcc
    14221 cgagccgaca tgacgacggc cagcacattt aaagcgttcc agcctagaaa cgagagtaag
    14281 aagatttccg atgatgatca acaagcagtg caagcgtcgt tgcatgcgaa ccctgcagcg
    14341 atgtctcgtt ccatgaattt ccacgagagt aacggatccg gtaccatggt gagcaagggc
    14401 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
    14461 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
    14521 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
    14581 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
    14641 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
    14701 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
    14761 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
    14821 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
    14881 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
    14941 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
    15001 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
    15061 accgccgccg ggatcactct cggcatggac gagctgtaca agtaagagct
//