| 质粒名称: | pDJ73 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001153 |
| 产品用途: | Mammalian mTOR-responsive SEAP expression vector |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | mTOR-responsive |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | SEAP |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Fluorescent protein-based ticker tapes for multiplexed recordings of transcriptional histories in single cells in culture and in vivo
Wang R., Jiang J., Li Z., Liu T., Wang Y., Xie M., Piatkevich K.D.
Preprint
|
pDJ73
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pDJ73载体质粒基本信息
pDJ73质粒图谱载体图谱和pDJ73载体序列质粒序列多克隆位点信息
pDJ73质粒载体简介
pDJ73质粒序列载体序列
LOCUS pDJ73 5126 bp DNA circular SYN 08-APR-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5126)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Aug 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5126
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 6..121
/label=important segment
misc_feature 6..15
/label=SRE-1
/note="sterol regulatory element-1"
misc_feature 27..36
/label=SRE-1
/note="sterol regulatory element-1"
misc_feature 48..57
/label=SRE-1
/note="sterol regulatory element-1"
misc_feature 91..99
/label=E-box
misc_feature 113..121
/label=E-box
misc_feature 129..277
/label=CMVmin
promoter 151..189
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 321..1841
/label=important segment
CDS 321..1823
/codon_start=1
/product="secreted alkaline phosphatase from human
placenta"
/label=SEAP
/note="The natural enzyme has a C-terminal transmembrane
sequence that is replaced by a GPI anchor."
/translation="MLLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQP
AQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDK
HVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVV
TTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRK
YMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTH
LMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESR
AYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKA
RDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFA
RGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
misc_feature 1076..1081
/label=putative TEAD-binding sites
polyA_signal 2883..3017
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3379..3967)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4434..4812)
/label=ISS
/note="immunostimulatory sequence from the AmpR gene;
contains unmethylated CpG dinucleotides in the context of
5'-AACGTT-3' (Sato et al., 1996)"
promoter complement(4997..5101)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacgtatcac cccacacatc tgacgtatca ccccacacat ctgacgtatc accccacaca
61 tctgacgtca ggatatcctg caggacatct atcacgtgac tgcaggacat ctatcacgtg
121 actgcaggtc gagctcggta cccgggtcga ggtaggcgtg tacggtggga ggcctatata
181 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
241 ctccatagaa gacaccggga ccgatccagc ctccgcggaa ttcgagctcg cccggggatc
301 ctctagagtc agcttcctgc atgctgctgc tgctgctgct gctgggcctg aggctacagc
361 tctccctggg catcatccca gttgaggagg agaacccgga cttctggaac cgcgaggcag
421 ccgaggccct gggtgccgcc aagaagctgc agcctgcaca gacagccgcc aagaacctca
481 tcatcttcct gggcgatggg atgggggtgt ctacggtgac agctgccagg atcctaaaag
541 ggcagaagaa ggacaaactg gggcctgaga tacccctggc catggaccgc ttcccatatg
601 tggctctgtc caagacatac aatgtagaca aacatgtgcc agacagtgga gccacagcca
661 cggcctacct gtgcggggtc aagggcaact tccagaccat tggcttgagt gcagccgccc
721 gctttaacca gtgcaacacg acacgcggca acgaggtcat ctccgtgatg aatcgggcca
781 agaaagcagg gaagtcagtg ggagtggtaa ccaccacacg agtgcagcac gcctcgccag
841 ccggcaccta cgcccacacg gtgaaccgca actggtactc ggacgccgac gtgcctgcct
901 cggcccgcca ggaggggtgc caggacatcg ctacgcagct catctccaac atggacattg
961 acgtgatcct aggtggaggc cgaaagtaca tgtttcgcat gggaacccca gaccctgagt
1021 acccagatga ctacagccaa ggtgggacca ggctggacgg gaagaatctg gtgcaggaat
1081 ggctggcgaa gcgccagggt gcccggtatg tgtggaaccg cactgagctc atgcaggctt
1141 ccctggaccc gtctgtgacc catctcatgg gtctctttga gcctggagac atgaaatacg
1201 agatccaccg agactccaca ctggacccct ccctgatgga gatgacagag gctgccctgc
1261 gcctgctgag caggaacccc cgcggcttct tcctcttcgt ggagggtggt cgcatcgacc
1321 atggtcatca tgaaagcagg gcttaccggg cactgactga gacgatcatg ttcgacgacg
1381 ccattgagag ggcgggccag ctcaccagcg aggaggacac gctgagcctc gtcactgccg
1441 accactccca cgtcttctcc ttcggaggct accccctgcg agggagctcc atcttcgggc
1501 tggcccctgg caaggcccgg gacaggaagg cctacacggt cctcctatac ggaaacggtc
1561 caggctatgt gctcaaggac ggcgcccggc cggatgttac cgagagcgag agcgggagcc
1621 ccgagtatcg gcagcagtca gcagtgcccc tggacgaaga gacccacgca ggcgaggacg
1681 tggcggtgtt cgcgcgcggc ccgcaggcgc acctggttca cggcgtgcag gagcagacct
1741 tcatagcgca cgtcatggcc ttcgccgcct gcctggagcc ctacaccgcc tgcgacctgg
1801 cgccccccgc cggcaccacc gacgccgcgc acccgggtta acccgtggtc cccgcgttgc
1861 ttcctctgct ggccgggacc ctgctgctgc tggagacggc cactgctccc tgagtgtccc
1921 gtccctgggg ctcctgcttc cccatcccgg agttctcctg ctccccacct cctgtcgtcc
1981 tgcctggcct ccagcccgag tcgtcatccc cggagtccct atacagaggt cctgccatgg
2041 aaccttcccc tccccgtgcg ctctggggac tgagcccatg acaccaaacc tgccccttgg
2101 ctgctctcgg actccctacc ccaaccccag ggactgcagg ttgtgccctg tggctgcctg
2161 caccccagga aaggaggggg ctcaggccat ccagccacca cctacagccc agtggcctcg
2221 agctgcagaa gcttttaaaa cagctctggg gttgtaccca ccccagaggc ccacgtggcg
2281 gctagtactc cggtattgcg gtacccttgt acgcctgttt tatactccct tcccgtaact
2341 tagacgcaca aaaccaagtt caatagaagg gggtacaaac cagtaccacc acgaacaagc
2401 acttctgttt ccccggtgat gtcgtataga ctgcttgcgt ggttgaaagc gacggatccg
2461 ttatccgctt atgtacttcg agaagcccag taccacctcg gaatcttcga tgcgttgcgc
2521 tcagcactca accccagagt gtagcttagg ctgatgagtc tggacatccc tcaccggtga
2581 cggtggtcca ggctgcgttg gcggcctacc tatggctaac gccatgggac gctagttgtg
2641 aacaaggtgt gaagagccta ttgagctaca taagaatcct ccggcccctg aatgcggcta
2701 atcccaacct cggagcaggt ggtcacaaac cagtgattgg cctgtcgtaa cgcgcaagtc
2761 cgtggcggaa ccgactactt tgggtgtccg tgtttccttt tattttattg tggctgctta
2821 tggtgacaat cacagattgt tatcataaag cgaattggat tgcggccgct taagctagct
2881 tcgatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
2941 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
3001 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
3061 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
3121 atcctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
3181 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
3241 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
3301 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgtcg
3361 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
3421 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
3481 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
3541 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
3601 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
3661 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
3721 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
3781 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
3841 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
3901 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
3961 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
4021 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
4081 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
4141 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
4201 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
4261 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
4321 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
4381 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtg cgcaacgttg
4441 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
4501 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
4561 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
4621 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
4681 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
4741 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
4801 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
4861 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
4921 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
4981 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
5041 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
5101 gcacatttcc ccgaaaagtg ccacct
//
pDJ73载体图谱质粒图谱pdf版和pDJ73载体序列质粒序列等相关资料下载
pDJ73质粒载体应用举例
- 上一篇：pDJ72
- 下一篇：没有了
