| 质粒名称: | Tet-O-FUW-Ascl1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 27150 |
| 存储实验室: | Marius Wernig |
|
载体骨架基本信息 |
|
| 载体名称: | TetO-FUW |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 8393 bp |
| 完整质粒大小: | 9089 bp |
| 载体类型: | 哺乳细胞表达，Lentiviral |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Ascl1 |
| 物种来源: | M. musculus (老鼠) |
| 插入大小: | 696 bp |
| 基因又名: | Mash1 |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoR1(未破坏) |
| 3' 克隆位点: | EcoR1(未破坏) |
| 5' 测序引物 | LNCX |
| 3' 测序引物 | WPRE-R |
|
文献引用方法 |
|
| 材料和方法部分: | Tet-O-FUW-Ascl1 was a gift from Marius Wernig (爱迪基因 plasmid # 27150) |
| 参考文献部分: | Direct conversion of fibroblasts to functional neurons by defined factors. Vierbuchen T, Ostermeier A, Pang ZP, Kokubu Y, Sudhof TC, Wernig M. Nature. 2010 Feb 25. 463(7284):1035-41. 10.1038/nature08797 PubMed 20107439 |
买家导航
Tet-O-FUW-Ascl1载体质粒基本信息
Tet-O-FUW-Ascl1质粒图谱载体图谱和Tet-O-FUW-Ascl1载体序列质粒序列多克隆位点信息
Tet-O-FUW-Ascl1质粒载体简介
Tet-O-FUW-Ascl1质粒序列载体序列
LOCUS Tet-O-FUW-Ascl1 9089 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Tet-O-FUW-Ascl1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9089)
AUTHORS Vierbuchen T, Ostermeier A, Pang ZP, Kokubu Y, Sudhof TC, Wernig M
TITLE Direct conversion of fibroblasts to functional neurons by defined
factors.
JOURNAL Nature. 2010 Feb 25. 463(7284):1035-41.
PUBMED 20107439
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|Tet-O-FUW-Ascl1|
FEATURES Location/Qualifiers
source 1..9089
/organism="synthetic DNA construct"
/mol_type="other DNA"
regulatory 10..19
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
misc_feature 736..1324
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(789..809)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(1207..1218)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(1327..1343)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1328..1344)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 1849..2029
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(2055..2072)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 2061..2285
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2331..2759
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2418..2437)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 2628..2649
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(2768..2788)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 2773..3102
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2953..3088
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3015..3034
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 3150..3197
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 3216..3590
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 3720..3841
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3757..3776)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3811..3830
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(3890..3906)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3890..3906)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3903..3925)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3914..3930
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3938..3968)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3983..4004
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4121..4138)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4292..4880)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4372..4391)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5051..5911)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5674..5693
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5912..6016)
/gene="bla"
/label=AmpR promoter
primer_bind complement(6091..6110)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 6282..6661
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 6663..6861
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 6816..6836
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 6879..7059
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 7106..7231
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 7724..7957
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 8484..8601
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind 8655..8925
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
promoter 8958..8995
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 8992..9016
/label=LNCX
/note="Human CMV promoter, forward primer"
ORIGIN
1 aattcgctag ccaccatgga gagctctggc aagatggaga gtggagccgg ccagcagccg
61 cagcccccgc agcccttcct gcctcccgca gcctgcttct ttgcgaccgc ggcggcggcg
121 gcagcggcgg cggccgcggc agctcagagc gcgcagcagc aacagccgca ggcgccgccg
181 cagcaggcgc cgcagctgag cccggtggcc gacagccagc cctcaggggg cggtcacaag
241 tcagcggcca agcaggtcaa gcgccagcgc tcgtcctctc cggaactgat gcgctgcaaa
301 cgccggctca acttcagcgg cttcggctac agcctgccac agcagcagcc ggccgccgtg
361 gcgcgccgca acgagcgcga gcgcaaccgg gtcaagttgg tcaacctggg ttttgccacc
421 ctccgggagc atgtccccaa cggcgcggcc aacaagaaga tgagcaaggt ggagacgctg
481 cgctcggcgg tcgagtacat ccgcgcgctg cagcagctgc tggacgagca cgacgcggtg
541 agcgctgcct ttcaggcggg cgtcctgtcg cccaccatct cccccaacta ctccaacgac
601 ttgaactcta tggcgggttc tccggtctcg tcctactcct ccgacgaggg atcctacgac
661 cctcttagcc cagaggaaca agagctgctg gactttacca actggttctg agaattcgat
721 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
781 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
841 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
901 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
961 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
1021 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
1081 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
1141 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
1201 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
1261 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
1321 ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
1381 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
1441 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
1501 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
1561 gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac
1621 acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca
1681 gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct
1741 gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc
1801 cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg
1861 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
1921 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
1981 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta
2041 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
2101 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
2161 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
2221 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
2281 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
2341 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
2401 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
2461 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
2521 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
2581 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
2641 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
2701 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
2761 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
2821 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
2881 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
2941 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
3001 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
3061 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
3121 atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg
3181 gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc
3241 cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt
3301 tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt
3361 tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc
3421 gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg
3481 cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc
3541 gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac
3601 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
3661 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
3721 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
3781 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
3841 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
3901 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
3961 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
4021 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
4081 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
4141 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
4201 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
4261 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
4321 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
4381 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
4441 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
4501 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
4561 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
4621 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
4681 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
4741 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
4801 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
4861 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
4921 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
4981 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
5041 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
5101 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
5161 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
5221 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
5281 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
5341 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
5401 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
5461 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
5521 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
5581 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
5641 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
5701 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
5761 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
5821 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
5881 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
5941 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
6001 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
6061 atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag
6121 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
6181 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
6241 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
6301 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
6361 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
6421 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
6481 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
6541 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
6601 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
6661 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
6721 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
6781 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
6841 gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga
6901 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
6961 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
7021 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
7081 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
7141 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
7201 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
7261 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
7321 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
7381 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
7441 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
7501 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
7561 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
7621 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
7681 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
7741 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
7801 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
7861 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
7921 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
7981 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
8041 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
8101 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
8161 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
8221 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
8281 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
8341 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
8401 acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga
8461 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
8521 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
8581 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta
8641 ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
8701 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
8761 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
8821 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
8881 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
8941 ctcggtaccc gggtcgagta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt
9001 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
9061 ccgggaccga tccagcctcc gcggccccg
//
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