pSL1501

LOCUS       pSL1501            7041 bp DNA     circular SYN 11-FEB-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Aug 30, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     primer_bind     123..164
                     /label=oSCL1800
     misc_feature    143..725
                     /label=CMV
     primer_bind     complement(699..730)
                     /label=OXB018
     primer_bind     706..741
                     /label=oSCL1791
     primer_bind     709..729
                     /label=oSCL1013
     misc_feature    731..732
                     /label=linker
     primer_bind     736..777
                     /label=oSCL2270
     misc_feature    759..771
                     /label=linker
     misc_feature    777..795
                     /label=MS2 box
     misc_feature    801..810
                     /label=linker
     misc_feature    816..834
                     /label=MS2 box
     misc_feature    840..849
                     /label=linker
     misc_feature    840..849
                     /label=linker
     misc_feature    848..878
                     /label=hairpin
     misc_feature    854..872
                     /label=MS2 box
     misc_feature    877..889
                     /label=linker
     misc_feature    888..918
                     /label=hairpin
     misc_feature    894..912
                     /label=MS2 box
     primer_bind     complement(911..928)
                     /label=oSCL2271
     primer_bind     920..946
                     /label=oSCL2270
     misc_feature    922..923
                     /label=linker
     misc_feature    928..940
                     /label=linker
     misc_feature    946..964
                     /label=MS2 box
     misc_feature    970..979
                     /label=linker
     misc_feature    985..1003
                     /label=MS2 box
     misc_feature    1009..1018
                     /label=linker
     misc_feature    1009..1018
                     /label=linker
     misc_feature    1017..1047
                     /label=hairpin
     misc_feature    1023..1041
                     /label=MS2 box
     misc_feature    1046..1058
                     /label=linker
     misc_feature    1057..1087
                     /label=hairpin
     misc_feature    1063..1081
                     /label=MS2 box
     primer_bind     complement(1080..1109)
                     /label=oSCL2271
     misc_feature    1091..1096
                     /label=linker
     primer_bind     1104..1125
                     /label=Oscl31-3
     misc_feature    1105..1108
                     /label=New Feature
     misc_feature    1105..1108
                     /label=New Feature
     misc_feature    1108
                     /label=EGFP
                     /note="note=""Fragment extracted from OWS233-Kpn1-EGFP-Xba1
                     [758  nt] : (KpnI[4] / XbaI[766])"
     primer_bind     1111..1125
                     /label=oSCL775
     primer_bind     1113..1125
                     /label=oSCL456
     misc_feature    1119..2678
                     /label=SEAP
     primer_bind     complement(1307..1343)
                     /label=oSCL545
     primer_bind     1324..1352
                     /label=oSCL544
     primer_bind     complement(2140..2171)
                     /label=oSCL546
     primer_bind     2152..2184
                     /label=oSCL547
     primer_bind     2237..2267
                     /label=Oscl50-1
     primer_bind     complement(2237..2267)
                     /label=Oscl50-2
     primer_bind     complement(2626..2678)
                     /label=oSCL795
     primer_bind     complement(2660..2698)
                     /label=oSCL2503
     primer_bind     complement(2660..2675)
                     /label=oSCL475
     primer_bind     complement(2662..2678)
                     /label=Oscl31-4
     primer_bind     complement(2662..2678)
                     /label=oSCL457
     primer_bind     complement(2662..2678)
                     /label=oSCL548
     primer_bind     complement(2662..2678)
                     /label=oSCL776
     primer_bind     complement(2662..2678)
                     /label=oSCL083
     polyA_signal    2670..2678
                     /label=SV40 late polyA
     primer_bind     2672..2713
                     /label=OXB007
     primer_bind     2676..2713
                     /label=oSCL1795
     primer_bind     complement(2676..2698)
                     /label=oSCL1849
     polyA_signal    2685..2806
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2696..2715)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2709..2729
                     /label=PSG5-R(SV40-R)
     primer_bind     2750..2769
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2777..2806)
                     /label=OXB016
     primer_bind     complement(2781..2806)
                     /label=OXB008
     primer_bind     complement(2790..2812)
                     /label=oSCL1801
     primer_bind     complement(2790..2806)
                     /label=oSCL1174
     primer_bind     complement(2790..2806)
                     /label=oSCL1796
     misc_feature    2811..3398
                     /label=CMV
     primer_bind     2811..2832
                     /label=oSCL1800
     primer_bind     complement(3367..3398)
                     /label=OXB018
     primer_bind     3374..3398
                     /label=oSCL1791
     primer_bind     3377..3397
                     /label=oSCL1013
     primer_bind     3399..3418
                     /label=T7
     primer_bind     3405..3454
                     /label=oSCL2413
     primer_bind     3428..3454
                     /label=oSCL2381
     CDS             3437..3598
                     /codon_start=1
                     /label=MS2-N55K
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGIAEWISSNSRSQAYKVT
                     CSVRQSSAG"
     primer_bind     complement(3570..3618)
                     /label=oSCL2481
     CDS             3599..4009
                     /codon_start=1
                     /label=LOV2
                     /translation="LERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGRNCRF
                     LQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQYFIG
                     VQLDGTEHVRDAAEREGVMLIKKTAENIDEAAG"
     primer_bind     3599..3618
                     /label=oSCL2482
     primer_bind     3603..3645
                     /label=oSCL2408
     primer_bind     complement(3603..3622)
                     /label=oSCL2407
     CDS             3623..3625
                     /codon_start=1
                     /label=V416I
                     /translation="V"
     primer_bind     3939..3981
                     /label=oSCL2410
     primer_bind     complement(3939..3958)
                     /label=oSCL2409
     CDS             3959..3961
                     /codon_start=1
                     /label=G528A
                     /translation="G"
     primer_bind     3969..4012
                     /label=oSCL2412
     primer_bind     complement(3969..3988)
                     /label=oSCL2411
     CDS             3989..3991
                     /codon_start=1
                     /label=N538E
                     /translation="N"
     CDS             4010..4237
                     /codon_start=1
                     /label=MS2-N55K
                     /translation="QKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATN
                     SDCELIVKAMQGLLKDGNPIPSAIAANSGIY"
     primer_bind     complement(4218..4260)
                     /label=oSCL2415
     primer_bind     complement(4218..4240)
                     /label=oSCL2383
     primer_bind     complement(4218..4237)
                     /label=oSCL2382
     primer_bind     4218..4237
                     /label=oSCL2384
     misc_feature    4247..4295
                     /label=HSV TK poly(A) signal
     primer_bind     complement(4271..4303)
                     /label=oSCL1799
     repeat_region   4304..4444
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4304..4433
                     /label=AAV2 ITR
     rep_origin      4519..4974
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4606..4625)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4816..4837
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4991..5010)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5110..5132
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5170..5188)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5256..5360
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5361..6221
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5579..5598)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6392..6980
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6770..6797)
                     /label=oSCL2481
     primer_bind     6881..6900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggcctcta gagttgacat tgattattga ctagttatta atagtaatca
      181 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      241 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      301 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      361 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      421 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      481 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      541 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      601 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      661 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc cttgaaccgg tggatccggt ctcaaactgc aacaaacaaa tcaccaccgt
      781 gaggatcacc cacggtggtg aaagccttag cctagccgtg aggatcaccc acggtaggca
      841 caagaaattc tacccgtgag gatcacccac gggtagaatc acaaagacta cagccgtgag
      901 gatcacccac ggctgtagac tcaaactgca acaaacaaat caccaccgtg aggatcaccc
      961 acggtggtga aagccttagc ctagccgtga ggatcaccca cggtaggcac aagaaattct
     1021 acccgtgagg atcacccacg ggtagaatca caaagactac agccgtgagg atcacccacg
     1081 gctgtagact caaactggag acgaagctta gcgccaccat gctgctgctg ctgctgctgc
     1141 tgggcctgag gctacagctc tccctgggca tcatcccagt tgaggaggag aacccggact
     1201 tctggaaccg cgaggcagcc gaggccctgg gtgccgccaa gaagctgcag cctgcacaga
     1261 cagccgccaa gaacctcatc atcttcctgg gcgatgggat gggggtgtct acggtgacag
     1321 ctgccagaat cctaaaaggg cagaagaagg acaaactggg gcctgagata cccctggcca
     1381 tggaccgctt cccatatgtg gctctgtcca agacatacaa tgtagacaaa catgtgccag
     1441 acagtggagc cacagccacg gcctacctgt gcggggtcaa gggcaacttc cagaccattg
     1501 gcttgagtgc agccgcccgc tttaaccagt gcaacacgac acgcggcaac gaggtcatct
     1561 ccgtgatgaa tcgggccaag aaagcaggga agtcagtggg agtggtaacc accacacgag
     1621 tgcagcacgc ctcgccagcc ggcacctacg cccacacggt gaaccgcaac tggtactcgg
     1681 acgccgacgt gcctgcctcg gcccgccagg aggggtgcca ggacatcgct acgcagctca
     1741 tctccaacat ggacattgac gtgatcctag gtggaggccg aaagtacatg tttcgcatgg
     1801 gaaccccaga ccctgagtac ccagatgact acagccaagg tgggaccagg ctggacggga
     1861 agaatctggt gcaggaatgg ctggcgaagc gccagggtgc ccggtatgtg tggaaccgca
     1921 ctgagctcat gcaggcttcc ctggacccgt ctgtgaccca tctcatgggt ctctttgagc
     1981 ctggagacat gaaatacgag atccaccgag actccacact ggacccctcc ctgatggaga
     2041 tgacagaggc tgccctgcgc ctgctgagca ggaacccccg cggcttcttc ctcttcgtgg
     2101 agggtggtcg catcgaccat ggtcatcatg aaagcagggc ttaccgggca ctgactgaga
     2161 ccatcatgtt cgacgacgcc attgagaggg cgggccagct caccagcgag gaggacacgc
     2221 tgagcctcgt cactgccgac cactcccacg tgttctcctt cggaggctac cccctgcgag
     2281 ggagctccat cttcgggctg gcccctggca aggcccggga caggaaggcc tacacggtcc
     2341 tcctatacgg aaacggtcca ggctatgtgc tcaaggacgg cgcccggccg gatgttaccg
     2401 agagcgagag cgggagcccc gagtatcggc agcagtcagc agtgcccctg gacgaagaga
     2461 cccacgcagg cgaggacgtg gcggtgttcg cgcgcggccc gcaggcgcac ctggttcacg
     2521 gcgtgcagga gcagaccttc atagcgcacg tcatggcctt cgccgcctgc ctggagccct
     2581 acaccgcctg cgacctggcg ccccccgccg gcaccaccga cgccgcgcac ccgggttaca
     2641 gcagagtcgg ggcggccggc cgcttcgagc agacatgact cgagtaagat acattgatga
     2701 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2761 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttacgc gttgacattg
     2821 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     2881 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2941 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     3001 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     3061 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     3121 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     3181 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     3241 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     3301 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3361 taggcgtgta cggtgggagg tctatataag cagagctcta atacgactca ctatagggaa
     3421 gctttaagcc accatggcta gcaacttcac acagttcgtg ctggtggaca acggcggcac
     3481 cggcgacgtg accgtggccc ctagcaactt cgccaacggc atcgccgagt ggatcagcag
     3541 caacagcaga agccaagcct acaaggtgac ctgcagcgtg agacagagca gcgccggcct
     3601 ggagagaatc gagaagaact tcgtgatcac cgaccctaga ctgcccgaca accccatcat
     3661 cttcgctagc gacagcttcc tgcagctgac cgagtacagc agagaggaga tcctgggcag
     3721 aaactgcaga ttcctgcaag gccccgagac cgacagagcc accgtgagaa agatcagaga
     3781 cgccatcgac aatcagaccg aggtgaccgt gcagctgatc aactacacca agagcggcaa
     3841 gaagttctgg aacctgttcc acctgcagcc catgagagat cagaagggcg acgtgcagta
     3901 cttcatcggc gtgcagctgg acggcaccga gcacgtgaga gacgccgccg agagagaggg
     3961 cgtgatgctg atcaagaaga ccgccgagaa catcgacgag gccgccgggc agaagagaaa
     4021 gtacaccatc aaagtcgagg tgcccaaggt ggccacccaa accgtgggcg gcgtggagct
     4081 gcccgtggcc gcctggagaa gctacctgaa catggagctg accatcccca tcttcgccac
     4141 caacagcgac tgcgagctga tcgtgaaggc catgcaaggc ctgctgaagg acggcaaccc
     4201 cattcctagc gccatcgctg ctaacagcgg catttattaa caattgcggc aataaaaaga
     4261 cagaataaaa cgcacgggtg ttgggtcgtt tgttcgcggc cgcaggaacc cctagtgatg
     4321 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     4381 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
     4441 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     4501 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4561 ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct
     4621 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4681 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     4741 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4801 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg
     4861 gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc
     4921 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
     4981 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     5041 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     5101 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     5161 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     5221 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5281 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5341 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5401 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5461 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5521 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5581 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5641 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5701 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5761 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5821 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5881 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5941 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     6001 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     6061 gagccggtga gcgtggaagc cgcggtatca ttgcagcact ggggccagat ggtaagccct
     6121 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     6181 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6241 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6301 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6361 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6421 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6481 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     6541 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6601 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6661 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6721 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6781 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7021 gctggccttt tgctcacatg t
//