pCN5-mBiyu-PBP2

LOCUS      pCN5-mBiyu-PBP2           10005 bp DNA     circular SYN 13-MAY-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10005)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Sep 28, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..2089
                     /label=pT181cop-wt repC
     CDS             2372..3022
                     /codon_start=1
                     /label=Cm
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     promoter        3362..3466
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3467..4327
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4498..5086
                     /direction=RIGHT
                     /label=ColE1ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5252..5314
                     /label=Pspac
                     /note="Pspac"
     misc_feature    5256..5261
                     /label=predicted TTGACA
     misc_feature    5276..5284
                     /label=predicted TATA box
     misc_feature    5326..5337
                     /label=RBS site
     CDS             5338..6084
                     /codon_start=1
                     /label=mBiyu
                     /translation="MVSKGEEDNMASYGALLFREKIPYVVEMEGDVEGMKFSVRGKGHG
                     DANTGKIEASFICTTGELPVPWSSILTTVTYGAQCFAKYPNDIKDYLKSTMPEGYVQER
                     TITFENDGIYKTRAEVTYEKGSVYNRVTLNGSGFKKGGNILGKKLEFNFNASRTYVLPD
                     VENNGIKCYINIRHNVIGGGQIRAEHQQLNTPLGGGPVDIPHYHAIQTHTILSKDPKET
                     RDHMNVVEVFRAIDCKTAYAGMDELYK"
     primer_bind     6085..6138
                     /label=sGFP-pbp2-P5
     CDS             6115..8295
                     /codon_start=1
                     /label=PBP2
                     /translation="TENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFFVV
                     LLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDVPKS
                     MKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLSQHKS
                     IGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKDLNLAE
                     EAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDLKANLVN
                     RTPEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTNMDKDVQK
                     TLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATDPHPTGSSL
                     KPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDALRQSFNIPA
                     LKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQLASAFAAIANG
                     GTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKPYGSAYGHGVSG
                     VNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGFSKVKQYGENSFV
                     GHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQDNNTTNRSTHGGS
                     DTSANSSGTAQSNNNTRSQQSRNSGGLTGIFN"
     primer_bind     6115..6131
                     /label=sgfp-pbp2-P5-new
     misc_feature    complement(8332..8351)
                     /label=pBCB13DOWN_seq_P1
                     /note="pBCB13DOWN_seq_P1"
     misc_feature    complement(8464..8480)
                     /label=check-pBCB-12r
                     /note="pBCB-12"
     misc_feature    8604..9686
                     /label=LacI
                     /note="LacI"
     misc_feature    8723..8744
                     /label=LacI_Seq_P1
                     /note="LacI_Seq_P1"
     misc_feature    9127..9150
                     /label=LacI_Spa-P1
                     /note="LacI_Spa-P1   "
     primer_bind     complement(9426..9445)
                     /label=Check-pCN4-lacI-R
     primer_bind     complement(9667..9707)
                     /label=pCN4-FtsZ-GFP-LacI-R
     misc_feature    9687..9692
                     /label=Feature 13
     terminator      9688..10003
                     /label=transcription terminator
     primer_bind     complement(9750..9766)
                     /label=1980 - pCNseqDWRev
     primer_bind     complement(9762..9781)
                     /label=2172 - pCNseqDWRev2
ORIGIN
        1 cctttgcgga aagagttaat aagttaacag aagatgaacc aaaactaaat ggtttagcag
       61 gaaacttaga taaaaaaatg aatccagaat tatattcaga acaggaacag caacaagaac
      121 aacaaaagaa tcaaaaacga gatagaggta tgcacttata gaacatgcat ttatgccgag
      181 aaaacttatt ggttggaatg ggctatgtgt tagctaactt gttagcgagt tggttggact
      241 tgaattggga ttaatcccaa gaaagtacca actcaacaac acataaagcc ctgtaggttc
      301 cgaccaataa ggaaattgga ataaagcaat aaaaggagtt gaagaaatga aattcagaga
      361 agcctttgag aattttataa caagtaagta tgtacttggt gttttagtag ttttaactgt
      421 ttaccagata atacaaatgc ttaaataaaa aaagacttga tctgattaga ccaaatcttt
      481 tgatagtgtt atattaataa caaaataaaa aggagtcgct cacgccctga ccaaagtttg
      541 tgaacgacat cattcaaaga aaaaaacact gagttgtttt tataatcttg tatatttaga
      601 tattaaacga tatttaaata tacatcaaga tatatatttg ggtgagcgat tccttaaacg
      661 aaattgagat taaggagtcg attttttatg tataaaaaca atcatgcaaa tcattcaaat
      721 catttggaaa atcacgattt agacaatttt tctaaaaccg gctactctaa tagccggttg
      781 gacgcacata ctgtgtgcat atctgatcca aaattaagtt ttgatgcaat gacgatcgtt
      841 ggaaatctca accgagacaa cgctcaagcc ctttctaaat ttatgagtgt agagccccaa
      901 ataagacttt gggatattct tcaaacaaag tttaaagcta aagcacttca agaaaaagtt
      961 tatattgaat atgacaaagt gaaagcagat agttgggata gacgtaatat gcgtattgaa
     1021 tttaatccaa acaaacttac acgagatgaa atgatttggt taaaacaaaa tataataagc
     1081 tacatggaag atgacggttt tacaagatta gatttagcct ttgattttga agatgatttg
     1141 agtgactact atgcaatgtc tgataaagca gttaagaaaa ctatttttta tggtcgtaat
     1201 ggtaagccag aaacaaaata ttttggcgtg agagatagta atagatttat tagaatttat
     1261 aataaaaagc aagaacgtaa agataatgca gatgctgaag ttatgtctga acatttatgg
     1321 cgtgtagaaa tcgaacttaa aagagatatg gtggattact ggaatgattg ctttagtgat
     1381 ttacatatct tgcaaccaga ttggaaaact atccaacgca ctgcggatag agcaatagtt
     1441 tttatgttat tgagtgatga agaagaatgg ggaaagcttc acagaaattc tagaacaaaa
     1501 tataagaatt tgataaaaga aatttcgcca gtcgatttaa cggacttaat gaaatcgact
     1561 ttaaaagcga acgaaaaaca attgcaaaaa caaatcgatt tttggcaaca tgaatttaaa
     1621 ttttggaaat agtgtacata ttaatattac tgaacaaaaa tgatatattt aaactattct
     1681 aatttaggag gattttttta tgaagtgtct atttaaaaat ttggggaatt tatatgaggt
     1741 gaaagaataa tttaccccta taaactttag tcacctcaag taaagaggta aaattgttta
     1801 gtttatataa aaaatttaaa ggtttgtttt atagcgtttt attttggctt tgtattcttt
     1861 cattttttag tgtattaaat gaaatggttt taaatgtttc tttacctgat attgcaaatc
     1921 attttaatac tactcctgga attacaaact gggtaaacac tgcatatatg ttaacttttt
     1981 cgataggaac agcagtatat ggaaaattat ctgattatat aaatataaaa aaattgttaa
     2041 ttattggtat tagtttgagc tgtcttggtt cattgattgc ttttattggg cccacctagg
     2101 tattatcaag ataagaaaga aaaggatttt tcgctacgct caaatccttt aaaaaaacac
     2161 aaaagaccac attttttaat gtggtctttt attcttcaac taaagcaccc attagttcaa
     2221 caaacgaaaa ttggataaag tgggatattt ttaaaatata tatttatgtt acagtaatat
     2281 tgacttttaa aaaaggattg attctaatga agaaagcaga caagtaagcc tcctaaattc
     2341 actttagata aaaatttagg aggcatatca aatgaacttt aataaaattg atttagacaa
     2401 ttggaagaga aaagagatat ttaatcatta tttgaaccaa caaacgactt ttagtataac
     2461 cacagaaatt gatattagtg ttttataccg aaacataaaa caagaaggat ataaatttta
     2521 ccctgcattt attttcttag tgacaagggt gataaactca aatacagctt ttagaactgg
     2581 ttacaatagc gacggagagt taggttattg ggataagtta gagccacttt atacaatttt
     2641 tgatggtgta tctaaaacat tctctggtat ttggactcct gtaaagaatg acttcaaaga
     2701 gttttatgat ttataccttt ctgatgtaga gaaatataat ggttcgggga aattgtttcc
     2761 caaaacacct atacctgaaa atgctttttc tctttctatt attccatgga cttcatttac
     2821 tgggtttaac ttaaatatca ataataatag taattacctt ctacccatta ttacagcagg
     2881 aaaattcatt aataaaggta attcaatata tttaccgcta tctttacagg tacatcattc
     2941 tgtttgtgat ggttatcatg caggattgtt tatgaactct attcaggaat tgtcagatag
     3001 gcctaatgac tggcttttat aatatgagat aatgccgact gtacttttta cagtcggttt
     3061 tctaatgtca ctaacctgcc ccgttagttg aagaaggccg cggcctcgag cggccgcata
     3121 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     3181 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     3241 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     3301 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     3361 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     3421 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     3481 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     3541 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     3601 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     3661 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     3721 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     3781 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     3841 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     3901 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     3961 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     4021 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     4081 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     4141 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     4201 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     4261 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     4321 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     4381 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     4441 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4501 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4561 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4621 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     4681 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4741 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4801 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4861 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4921 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4981 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5041 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5101 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     5161 atcccctgat tctgtggata accgtattac cgcctttgag tgagctggcg gccgctgcat
     5221 gcgctaaaat tcctgaaaaa ttttgcaaaa agttgttgac tttatctaca aggtgtggca
     5281 taatgtgtgg aattgtgagc ggataacaat taagcttttc ccgggaggag gtaccttatg
     5341 gtgagtaaag gtgaggagga taatatggca tcttatggtg cattattatt tagagagaag
     5401 ataccttatg tagtagaaat ggaaggtgat gtcgagggaa tgaaattttc agttagaggt
     5461 aaaggccatg gcgatgctaa cactggcaag attgaggcat cttttatttg tacaactggt
     5521 gaattaccgg tgccatggag ttcaatctta acgacggtaa cttatggcgc tcaatgcttt
     5581 gcaaaatatc cgaatgacat taaagattat ttaaagtcaa ctatgccaga aggttatgta
     5641 caagaacgta caattacatt tgaaaatgat ggaatctata agacacgtgc agaggttacg
     5701 tacgaaaaag gttcagtcta caatagagtt acgttaaatg gttctggatt taagaaaggc
     5761 ggtaacattt taggcaagaa attggagttt aattttaatg cttcacgtac gtatgttttg
     5821 cctgatgtag aaaataacgg aatcaaatgt tacattaaca ttagacataa cgttattggt
     5881 ggtggtcaaa ttagagctga acatcaacaa ttaaacactc ctttaggtgg tggaccagtg
     5941 gatataccac attatcatgc aattcaaaca catacaattt taagtaaaga tcctaaagaa
     6001 actagagatc atatgaatgt agttgaagta tttagagcaa tagattgcaa aacggcatat
     6061 gcaggaatgg atgaattata taaaggtgga ggcggttctg gcggaggtgg ctctacggaa
     6121 aacaaaggat cttctcagcc taagaaaaac ggtaataatg gtgggaaatc caactcaaaa
     6181 aagaatagaa atgtgaagag aacgattatt aagattattg gcttcatgat tattgcattt
     6241 ttcgttgttc ttttactagg tatcttattg tttgcttatt atgcttggaa agcacctgct
     6301 tttaccgaag ctaaattaca agatccgatt cctgcaaaga tatatgacaa gaacggagaa
     6361 cttgttaaaa cattagataa tggccaaaga catgagcatg taaatttaaa agacgtgccg
     6421 aaatcaatga aagacgcagt acttgcaact gaagacaatc gtttctacga acatggcgca
     6481 cttgattata aacgtttatt cggtgcaatt ggtaagaact tgactggtgg atttggttct
     6541 gaaggtgcct caacattaac acaacaagtt gttaaagatg catttttatc acaacataaa
     6601 tctattggac gtaaagctca agaagcatac ttatcatatc gtttagaaca agagtatagt
     6661 aaagatgata tcttccaagt atatctaaac aaaatttact attctgatgg cgtaacaggt
     6721 attaaagctg ctgctaagta ttactttaat aaagatttaa aagatttaaa cttagcggaa
     6781 gaagcttatt tagccggttt acctcaggtt ccaaacaact ataatattta tgatcatcca
     6841 aaagctgctg aagatcgtaa aaacactgtt ttatacttaa tgcattatca taaacgcatt
     6901 acagataaac agtgggaaga tgctaagaaa atcgatttaa aagcgaactt agtaaatcgt
     6961 actcctgaag aacgtcaaaa cattgataca aatcaagatt ctgagtataa ttcatacgtt
     7021 aactttgtaa aatctgaatt aatgaataat aaagcattca aagatgaaaa tttaggtaat
     7081 gtattacaaa gtggtattaa aatttataca aacatggata aagatgttca aaaaacatta
     7141 caaaatgatg ttgataatgg tagcttctac aagaataaag accaacaagt tggtgcaaca
     7201 attcttgata gtaaaactgg tggtttagtt gctatatctg gtggacgtga tttcaaagac
     7261 gtcgttaaca gaaaccaagc aacagatcct caccctactg gttcatcttt aaaacctttc
     7321 ttagcgtatg gacctgccat tgaaaatatg aaatgggcaa caaaccatgc gattcaagat
     7381 gaatcttcat atcaagttga tggttctaca tttagaaact atgatacgaa gagtcacggt
     7441 actgtatcta tttatgatgc tttacgacaa agtttcaata tcccagcttt aaaagcttgg
     7501 caatcagtta agcaaaatgc tggtaatgat gcacctaaga aattcgctgc caaacttggc
     7561 ttaaactacg aaggcgatat tggtccatct gaagtacttg gtggttctgc ttcagaattc
     7621 tcaccaacac aattagcatc agcatttgct gcaatcgcta acggtggtac ttataacaac
     7681 gcgcattcaa ttcaaaaagt agttactcgt gatggtgaaa caatcgaata cgatcatact
     7741 agccataaag cgatgagtga ttacactgca tacatgttag ctgagatgct aaaaggtaca
     7801 tttaaaccat atggttctgc atatggccat ggtgtatctg gagtaaatat gggtgctaag
     7861 acaggtactg gtacttacgg tgctgaaact tattcacaat ataatttacc tgataatgca
     7921 gcgaaagacg tgtggattaa cggctttaca cctcaataca ctatgtcagt gtggatgggc
     7981 ttcagtaaag ttaaacaata tggtgaaaac tcatttgtgg gacatagcca acaagaatat
     8041 ccacagttct tatatgaaaa tgtgatgtca aaaatttcat ctagagatgg cgaagacttt
     8101 aaacgtccta gctcagtaag tggtagtatc ccatcaatca atgtttctgg tagtcaagat
     8161 aacaacacta caaatcgtag tacacacggt ggtagtgaca catcagcaaa cagcagtggt
     8221 actgcacaat caaataacaa tactagatct caacaatcta gaaacagcgg tggattaaca
     8281 ggtatattca actaactcga gtcgctccga ggcgccgcag ctaggcgatt tgttaactta
     8341 gatctttatc gatttgcatg caagctaatt cggtggaaac gaggtcatca tttccttccg
     8401 aaaaaacggt tgcatttaaa tcttacatat gtaatacttt caaagactac atttgtaaga
     8461 tttgatgttt gagtcggctg aaagatcgta cgtaccaata attgtttcgt gattgttcaa
     8521 gccataacac tgtagggata gtggaaagag tgcttcatct ggttacgatc aatcaaatat
     8581 tcaaacggag ggagacgatt ttgatgaaac cagtaacgtt atacgatgtc gcagagtatg
     8641 ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga
     8701 aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg
     8761 cagaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc
     8821 tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca
     8881 gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca
     8941 atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg
     9001 ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc
     9061 agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc
     9121 atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct
     9181 cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga
     9241 tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc
     9301 tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg
     9361 caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat
     9421 acgacgatac cgaagacagc tcatgttata tcccgccgtc aaccaccatc aaacaggatt
     9481 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg
     9541 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca
     9601 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     9661 tttcccgact ggaaagcggg cagtgagaat tcaggcgcgc ctattctaat gcataataaa
     9721 tactgataac atcttatatt ttgtattata ttttgtatta tcgttgacat gtataatttt
     9781 gatatcaaaa actgattttc cctctattat tttcgagatt tattttctta attctcttta
     9841 acaaactaga aatattgtat atacaaaaaa ttataaataa tagatgaata gtttaattat
     9901 aggtgttcat caatcgaaaa agcaacgtat cttatttaaa gtgcgttgct tttttctcat
     9961 ttataaggtt aaataattct catatatcaa gcaaagtgac aggcg
//