pCW57-CMV-ssRFP-GFP-KDEL

LOCUS       pCW57-CMV-ssRFP-GFP-KDEL                9213 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Fluorescence reporter to monitor ER-phagy activity.
ACCESSION   .
VERSION     .
KEYWORDS    pCW57-CMV-ssRFP-GFP-KDEL
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9213)
  AUTHORS   Chino H, Hatta T, Natsume T, Mizushima N
  TITLE     Intrinsically Disordered Protein TEX264 Mediates ER-phagy.
  JOURNAL   Mol Cell. 2019 Apr 11. pii: S1097-2765(19)30257-6. doi: 
            10.1016/j.molcel.2019.03.033.
  PUBMED    31006538
REFERENCE   2  (bases 1 to 9213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9213
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1724..1841
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1909..2179
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2212..2250
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2247..2271
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     sig_peptide     2350..2403
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
     CDS             2404..3078
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(2534..2552)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2802..2821
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2996..3019
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             3124..3840
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3124..3840
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3169..3190)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3430..3449)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3777..3798
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        3862..4372
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4291..4309
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4382..4978
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4382..4401)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4878..4898
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4979..5032
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5033..5779
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5819..5839)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6237..6248)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6453..6686
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6758..6879
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6795..6814)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6849..6868
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6919..7054
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6981..7000
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7054..7125
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7126..7986
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7344..7363)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8157..8745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8646..8665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8899..8916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9033..9054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9069..9099
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9107..9123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9112..9134
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9131..9147
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9131..9147
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9166..9186
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9168..9186
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacaat tctcgacctc gagttttaaa agaaaagggg
     1741 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     1801 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     1861 gcagagatcc actttggccg cgaatcgata tgtcctcgag tttaccactc cctatcagtg
     1921 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     1981 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2041 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     2101 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2161 tccctatcag tgatagagaa aagtgaaagt cgagctcggt acccgggtcg aggtaggcgt
     2221 gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
     2281 cgccatccac gctgttttga cctccataga agacaccggg accgatccag cctccgcggc
     2341 cccgaattca tgaagttatg catattactg gccgtcgtgg cctttgttgg cctctcgctc
     2401 gggatggcct cctccgagga cgtcatcaag gagttcatgc gcttcaaggt gcgcatggag
     2461 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     2521 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
     2581 atcctgtccc ctcagttcca gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     2641 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     2701 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     2761 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     2821 accatgggct gggaggcctc caccgagcgg atgtaccccg aggacggcgc cctgaagggc
     2881 gagatcaaga tgaggctgaa gctgaaggac ggcggccact acgacgccga ggtcaagacc
     2941 acctacatgg ccaagaagcc cgtgcagctg cccggcgcct acaagaccga catcaagctg
     3001 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg cgccgagggc
     3061 cgccactcca ccggcgccat cgatgaattc actagtgtta acggatccct cgagcggccg
     3121 cccatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     3181 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     3241 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     3301 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     3361 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     3421 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     3481 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     3541 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     3601 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     3661 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     3721 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     3781 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     3841 aaagatgagc tctaaggatc cggggttggg gttgcgcctt ttccaaggca gccctgggtt
     3901 tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg
     3961 gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt
     4021 gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc
     4081 ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg cagacggaca
     4141 gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata gcggctgctc
     4201 agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg ggtgtggggc
     4261 ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg
     4321 cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agcaattcac
     4381 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     4441 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     4501 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     4561 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     4621 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     4681 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     4741 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     4801 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     4861 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     4921 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccga
     4981 aggtagaggt tctctcctca cttgtggtga tgttgaagaa aaccctggtc caatgtctag
     5041 actggacaag agcaaagtca taaacggagc tctggaatta ctcaatggtg tcggtatcga
     5101 aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg
     5161 gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc tggacaggca
     5221 tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc
     5281 caagtcatac cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac
     5341 ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca
     5401 aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg
     5461 ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga cacctaccac
     5521 cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccggc agggagccga
     5581 acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg
     5641 cgaaagcggc gggccgaccg acgcccttga cgattttgac ttagacatgc tcccagccga
     5701 tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg attttgacct
     5761 tgacatgctc cccgggtaac taagtaagga tcgatccaag atatcgtatt cttaactatg
     5821 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     5881 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     5941 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6001 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     6061 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     6121 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     6181 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     6241 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     6301 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgttt
     6361 cgcctcggcg tccggactag aggtaccttt aagaccaatg acttacaagg cagctgtaga
     6421 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     6481 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     6541 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     6601 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6661 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     6721 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     6781 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6841 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     6901 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     6961 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     7021 ttccagaagt agtgaggagg cttttttgga ggcctttcaa atatgtatcc gctcatgaga
     7081 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     7141 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     7201 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     7261 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     7321 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     7381 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     7441 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     7501 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     7561 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     7621 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     7681 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     7741 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     7801 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     7861 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     7921 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     7981 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     8041 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     8101 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     8161 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     8221 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     8281 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     8341 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     8401 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     8461 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     8521 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     8581 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     8641 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     8701 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     8761 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     8821 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     8881 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     8941 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     9001 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     9061 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     9121 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     9181 taaagggaac aaaagctgga gctgcaagct taa
//