pMK33-V5-TurboID-GW

LOCUS        pMK33-V5-TurboID-GW       10972 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gateway destination vector to express N-terminal TurboID fusion 
            under metallothionein promoter, for making stable cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10972)
  TITLE     Drosophila gateway destination vectors to express TurboID fusion 
            proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMK33-V5-TurboID-GW
FEATURES             Location/Qualifiers
     source          1..10972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(624..641)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(795..1383)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(875..894)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1554..2345,2346..2414))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2415..2519)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2587..2605
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4001..4280
                     /label=copia promoter
                     /note="strong promoter from the Drosophila transposable 
                     element copia (Sinclair et al., 1986)"
     CDS             4326..5351
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4326..5351
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     intron          5714..5779
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5909..5929
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6354..6488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6391..6410)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6445..6464
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        6489..6915
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     CDS             6930..6971
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6978..7934
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    7968..8092
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(8117..8122,8123..8140,8141..8147)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             8201..8860
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(8267..8286)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             9202..9507
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     9386..9405
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(9548..9672)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa
       61 ggatcgctcg cggctcttac cagcctaact tcgatcattg gaccgctgat cgtcacggcg
      121 atttatgccg cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta
      181 taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga
      241 atggaagccg gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat
      301 tcttgcggag aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg
      361 ccatctccag cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg ccacgggtgc
      421 gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc cttactggtt
      481 agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg
      541 cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc
      601 ggaagtcagc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      661 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      721 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      781 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      841 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      901 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      961 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1021 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1081 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1141 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1201 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     1261 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1321 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1381 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     1441 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     1501 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     1561 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     1621 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     1681 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     1741 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     1801 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     1861 gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     1921 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     1981 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     2041 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     2101 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     2161 ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     2221 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     2281 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     2341 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     2401 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     2461 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     2521 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     2581 tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattcacatt tgtacgaatt
     2641 ttttttttat caaaagttcg agtttttcac caatttcctc atcaaccgag caaggcaaac
     2701 ggctttgaat aatatggtgt tatatataca tatatcaaat cgctgctgac tgcgtgattg
     2761 atggccccaa gattacatat tatcgaatca ggattcagaa ggagatcaat gtcaaatgcg
     2821 gacaggaaca tgaaagacgc ctgttatgcg caattaaaaa tttgggttta attgctgtgg
     2881 aaactgttgt tggcggcatc ttaagttcct gtttaacaac atcaactact tatgtacgta
     2941 gaagcgttta agccatttgc atacagatga gaactggctt ttgtgctaat cagtcaagat
     3001 gactccgatg atgatgactc attacctgac cagttttcgc tgctttcttt tcaacaacta
     3061 cttgtatatg tattgtatcc aatagcaata cattgaattt ccatggtcta gtcacgtatt
     3121 atcatttaat tgacaccaag tcgtgttatt gttgagctat cgagttcagc tcaaacattt
     3181 cttattccca tgaataagcc ggcaaaaata tgcaatctat gaaagttaat ataagcaaac
     3241 cttactttga ctcaatacca atgcactttg tgtcgatagg ttcacgcaat tgaggcgatt
     3301 attccgataa cccaagcgat tgactgttcc cgtttcgatt ccaattgaaa tttggaaatg
     3361 tacaatagtt ttgctatatg ctgtcaagta cgctcttatc ttctctgggt tttcttcaga
     3421 gtttcgaaac gcttcttctt ttttttgttt tttttttttt ggaatctcgt attttggaag
     3481 gggctcccct ctggaatttg ttacactgtc gttatcattg cgaacaagcg gcccgaagct
     3541 atcagcgact ttaacattta caatgcactt ttttacgacc aattaaatgt acattttcct
     3601 ttcttcgccc gttgataagc gaacgcgatg tggcgcaggc aatgtgttgc tcttgcgaca
     3661 caaacgcaat caaaatggat tcaatttcgc tttttcccag tgaaacgaag aacgaaccga
     3721 ccatcatgat atgctcctct gcatgttgcg tattgaatca atgacaattt caattaagcc
     3781 gcccgttcgt catgcgtttt cgtgcgcttc gaaatgctga taacgctgct gtcctccaac
     3841 tgctttgcat gtggacacaa ttccatttat ttaattcttt tatttggatc ggttaaatta
     3901 aaaagcgcct tgttacgcat ttaacgttgt ttccggtgcg tggtggtttc atgcttctgg
     3961 gaacggcaaa tgggtttagg attgggaacc cctcatcatc tgttggaata tactattcaa
     4021 cctacaaaag taacgttaaa caacactact ttatatttga tatgaatggc cacacctttt
     4081 atgccataaa acatattgta agagaatacc actcttttta ttccttcttt ccttcttgta
     4141 cgttttttgc tgtaagtagg tcgtggtgct ggtgttgcag ttgaaataac ttaaaatata
     4201 aatcataaaa ctcaaacata aacttgacta tttatttatt tattaagaaa ggaaatataa
     4261 attataaatt acaacaggtt atggacctgc agccaagctt ggcgctcgtc cgggggcaat
     4321 gagatatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt
     4381 tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
     4441 tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca
     4501 aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg
     4561 acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca
     4621 cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca
     4681 tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc
     4741 aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg
     4801 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg
     4861 atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt
     4921 tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg
     4981 aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt
     5041 tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat
     5101 cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg
     5161 ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat
     5221 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg
     5281 atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg
     5341 caaaggaata gagtagatgc cgaccgaaca agagctgatt tcgagaacgc ctcagccagc
     5401 aactcgcgcg agcctagcaa ggcaaatgcg agagaacggc cttacgcttg gtggcacagt
     5461 tctcgtccac agttcgctaa gctcgctcgg ctgggtcgcg ggagggccgg tcgcagtgat
     5521 tcaggccctt ctggattgtg ttggtcccca gggcacgatt gtcatgccca cgcactcggg
     5581 tgatctgact gatcccgcag attggagatc gccgcccgtg cctgccgatt gggtgcagat
     5641 ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat
     5701 ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct
     5761 aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa
     5821 tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc
     5881 tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa
     5941 ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct
     6001 tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat
     6061 ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt
     6121 ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg
     6181 tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt
     6241 gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     6301 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     6361 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6421 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6481 tctggatcaa ttcgttgcag gacaggatgt ggtgcccgat gtgactagct ctttgctgca
     6541 ggccgtccta tcctctggtt ccgataagag acccagaact ccggcccccc accgcccacc
     6601 gccaccccca tacatatgtg gtacgcaagt aagagtgcct gcgcatgccc catgtgcccc
     6661 accaagagct ttgcatccca tacaagtccc caaagtggag aaccgaacca attcttcgcg
     6721 ggcagaacaa aagcttctgc acacgtctcc actcgaattt ggagccggcc ggcgtgtgca
     6781 aaagaggtga atcgaacgaa agacccgtgt gtaaagccgc gtttccaaaa tgtataaaac
     6841 cgagagcatc tggccaatgt gcatcagttg tggtcagcag caaaatcaag tgaatcatct
     6901 cagtgcaact aaagggggga tccaccatgg gcaagcccat ccccaacccc ctgctgggcc
     6961 tggacagcac cgctagcaaa gacaatactg tgcctctgaa gctgatcgct ctcctggcta
     7021 atggcgagtt ccatagtggc gaacagctgg gagaaaccct gggcatgtcc agggccgcta
     7081 tcaacaagca cattcagact ctgcgcgact ggggcgtgga cgtgttcacc gtgcccggaa
     7141 agggctactc tctgcccgag cctatcccgc tgctgaacgc taaacagatt ctgggacagc
     7201 tggacggcgg gagcgtggca gtcctgcctg tggtcgactc caccaatcag tacctgctgg
     7261 atcgaatcgg cgagctgaag agtggggatg cttgcattgc agaatatcag caggcaggga
     7321 gaggaagcag agggaggaaa tggttctctc cttttggagc taacctgtac ctgagtatgt
     7381 tttggcgcct gaagcgggga ccagcagcaa tcggcctggg cccggtcatc ggaattgtca
     7441 tggcagaagc gctgcgaaag ctgggagcag acaaggtgcg agtcaaatgg cccaatgacc
     7501 tgtatctgca ggatagaaag ctggcaggca tcctggtgga gctggccgga ataacaggcg
     7561 atgctgcaca gatcgtcatt ggcgccggga ttaacgtggc tatgaggcgc gtggaggaaa
     7621 gcgtggtcaa tcagggctgg atcacactgc aggaagcagg gattaacctg gacaggaata
     7681 ctctggccgc tacgctgatc cgagagctgc gggcagccct ggaactgttc gagcaggaag
     7741 gcctggctcc atatctgcca cggtgggaga agctggataa cttcatcaat agacccgtga
     7801 agctgatcat tggggacaaa gagattttcg ggattagccg ggggattgat aaacagggag
     7861 ccctgctgct ggaacaggac ggagttatca aaccctggat gggcggagaa atcagtctgc
     7921 ggtctgccga aaagagcggt ggcagcggcg gtggggatcc gatatcaaca agtttgtaca
     7981 aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc
     8041 ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggccgc
     8101 attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag
     8161 ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
     8221 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     8281 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
     8341 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
     8401 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag
     8461 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
     8521 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     8581 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
     8641 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     8701 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     8761 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga
     8821 attacaacag tactgcgatg agtggcaggg cggggcgtaa agatctggat ccggcttact
     8881 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
     8941 tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg
     9001 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
     9061 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
     9121 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
     9181 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
     9241 tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg
     9301 atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg
     9361 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc
     9421 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
     9481 attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca
     9541 ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg
     9601 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt
     9661 gtacaaagtg gttgatatca ctagttctag tcgaccatga agatcaagat cattgccccg
     9721 ccagagcgca agtactctgt ctggatcggt ggctccatcc tggcttcgct gtccaccttc
     9781 cagcagatgt ggatctccaa gcaggagtac gacgagtccg gcccctccat tgtgcaccgc
     9841 aagtgcttct aagaaggatc gcttgtctgg gcaagaggat caggatcggg atggtcttga
     9901 ttctgctgga ggaggaggag gagaagtcga ggaagcagca gcgaaagtgc aagtgcgagt
     9961 ggtggaagtt tggagtgcag cacaacaaaa tcaacaacaa caccaactac aagatgaaaa
    10021 gagcggaacc acctgcacac catcatcact atcatcatcg ttttgggcgc atgttgtgtg
    10081 gttccagcgt attaatataa ttaatttatt ccacatgaga tatgatatga tatactatgt
    10141 attttttgtt ttttttttat ttgtaaacct ttaatataac aagaactaca aaaaatgaaa
    10201 atgagcgaaa atgcatattc tgccattcca cacacacacc aacaacaccc aacacacgca
    10261 cacccacaag cttacacaca cacattcgcg gcatgacaag gacatcaaga taaagaagaa
    10321 cttaaagaag atatttccca aagcgcaaaa agaacacaca cacattgcaa aacacaaaca
    10381 acacactagc gttttgtaca attcgtcagc aaccttatgt attatttttt aattatgatg
    10441 taattataaa caaagtgaaa caaaaatatg aaaacaaaaa ggaaaatcaa atctgtcttc
    10501 tctttctccc gctctcctcg ctctctgctg ctaacctcgc cctctcctct ctcatctttt
    10561 tgtctgtctc tcttccacat ttttgccggc cggcaaaata ataacccaca cacactcaca
    10621 cttggctgca gtttcgcgtg cgatattcac acacattcaa gcatacaagc atacatacat
    10681 atgtattttt tttttatttg tacacttttc taattgcatg cgtatcgatt gataagttta
    10741 cgcctgaaaa tgttaattaa aatgtgaaaa tgcaactgaa aaactgatga aatgaaacaa
    10801 caacaagcga acaatttgct acatgtgtat tgtctaacaa ccgttactgc aacggttgct
    10861 tcggaaaagg gtgaagagga agagggcacg aggtcgacta gagcggccgc cgacgcgagg
    10921 ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga tg
//