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pZsYellow1-N1载体 pZsYellow1N1质粒基本信息
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pZsYellow1-N1质粒 pZsYellow1N1载体简介
pZsYellow1-N1质粒序列 pZsYellow1N1载体序列
LOCUS pZsYellow1-N1 4709 bp ds-DNA circular SYN 19-OCT-2017
DEFINITION Vector for fusing ZsYellow1 to the C-terminus of a partner protein.
ACCESSION .
VERSION .
KEYWORDS pZsYellow1-N1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4709)
AUTHORS Clontech (TaKaRa)
TITLE Direct Submission
JOURNAL Exported Wednesday, February 19, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4709
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 61..364
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 591..671
/note="MCS"
/note="multiple cloning site of fluorescent protein
plasmids"
CDS 679..1374
/codon_start=1
/product="Zoanthus yellow fluorescent protein"
/note="ZsYellow1"
/note="mammalian codon-optimized"
/translation="MAHSKHGLKEEMTMKYHMEGCVNGHKFVITGEGIGYPFKGKQTIN
LCVIEGGPLPFSEDILSAGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWGRSFLFEDGAV
CICNVDITVSVKENCIYHKSIFNGVNFPADGPVMKKMTTNWEASCEKIMPVPKQGILKG
DVSMYLLLKDGGRYRCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKWQLTEH
AIAFPSALA"
polyA_signal 1497..1618
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1625..2080)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2107..2211
/gene="bla"
/note="AmpR promoter"
promoter 2213..2570
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2421..2556
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2605..3399
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin)"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_feature 3631..3678
/note="HSV TK poly(A) signal"
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 4007..4595
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
661 gatccaccgg tcgccaccat ggcccacagc aagcacggcc tgaaggagga gatgaccatg
721 aagtaccaca tggagggctg cgtgaacggc cacaagttcg tgatcaccgg cgagggcatc
781 ggctacccct tcaagggcaa gcagaccatc aacctgtgcg tgatcgaggg cggccccctg
841 cccttcagcg aggacatcct gagcgccggc ttcaagtacg gcgaccggat cttcaccgag
901 tacccccagg acatcgtgga ctacttcaag aacagctgcc ccgccggcta cacctggggc
961 cggagcttcc tgttcgagga cggcgccgtg tgcatctgta acgtggacat caccgtgagc
1021 gtgaaggaga actgcatcta ccacaagagc atcttcaacg gcgtgaactt ccccgccgac
1081 ggccccgtga tgaagaagat gaccaccaac tgggaggcca gctgcgagaa gatcatgccc
1141 gtgcctaagc agggcatcct gaagggcgac gtgagcatgt acctgctgct gaaggacggc
1201 ggccggtacc ggtgccagtt cgacaccgtg tacaaggcca agagcgtgcc cagcaagatg
1261 cccgagtggc acttcatcca gcacaagctg ctgcgggagg accggagcga cgccaagaac
1321 cagaagtggc agctgaccga gcacgccatc gccttcccca gcgccctggc ctgaagcggc
1381 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
1441 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
1501 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
1561 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag
1621 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
1681 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
1741 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
1801 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
1861 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
1921 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
1981 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
2041 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
2101 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
2161 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg
2221 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
2281 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
2341 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
2401 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
2461 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
2521 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa
2581 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
2641 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
2701 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
2761 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
2821 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
2881 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
2941 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
3001 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
3061 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
3121 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
3181 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
3241 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
3301 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
3361 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
3421 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
3481 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
3541 gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg
3601 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
3661 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
3721 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
3781 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
3841 tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
3901 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
3961 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
4021 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
4081 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
4141 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
4201 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
4261 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
4321 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
4381 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
4441 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
4501 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
4561 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
4621 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
4681 ctgtggataa ccgtattacc gccatgcat
//
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