pSL1477

LOCUS       pSL1477             6440 bp DNA     circular SYN 11-FEB-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6440)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Aug 30, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     primer_bind     123..164
                     /label=oSCL1800
     misc_feature    143..725
                     /label=CMV
     primer_bind     complement(699..730)
                     /label=OXB018
     primer_bind     706..749
                     /label=oSCL1791
     primer_bind     709..729
                     /label=oSCL1013
     misc_feature    731..732
                     /label=linker
     primer_bind     complement(734..748)
                     /label=Oscl28-2
     primer_bind     complement(735..748)
                     /label=8x R
     misc_feature    749..761
                     /label=linker
     misc_feature    767..785
                     /label=MS2 box
     misc_feature    791..800
                     /label=linker
     misc_feature    806..824
                     /label=MS2 box
     misc_feature    830..839
                     /label=linker
     misc_feature    830..839
                     /label=linker
     misc_feature    838..868
                     /label=hairpin
     misc_feature    844..862
                     /label=MS2 box
     misc_feature    867..879
                     /label=linker
     misc_feature    878..908
                     /label=hairpin
     misc_feature    884..902
                     /label=MS2 box
     misc_feature    912..917
                     /label=linker
     misc_feature    926..941
                     /label=linker
     primer_bind     927..943
                     /label=oSCL2478
     misc_feature    968..980
                     /label=linker
     misc_feature    986..1004
                     /label=MS2 box
     misc_feature    1010..1019
                     /label=linker
     misc_feature    1025..1043
                     /label=MS2 box
     misc_feature    1049..1058
                     /label=linker
     misc_feature    1049..1058
                     /label=linker
     misc_feature    1057..1087
                     /label=hairpin
     misc_feature    1063..1081
                     /label=MS2 box
     misc_feature    1086..1098
                     /label=linker
     misc_feature    1097..1127
                     /label=hairpin
     misc_feature    1103..1121
                     /label=MS2 box
     misc_feature    1131..1136
                     /label=linker
     misc_feature    1145..1160
                     /label=linker
     primer_bind     1146..1199
                     /label=oSCL2478
     primer_bind     1161..1198
                     /label=oSCL2476
     primer_bind     1171..1198
                     /label=oSCL2474
     primer_bind     1172..1199
                     /label=oSCL2040
     primer_bind     1172..1193
                     /label=oSCL2104
     primer_bind     1174..1198
                     /label=OGT146-F
     primer_bind     1175..1199
                     /label=olyl337
     primer_bind     1175..1199
                     /label=oSCL2063
     primer_bind     1175..1198
                     /label=OGT147-F
     misc_feature    1181..1240
     primer_bind     1240..1264
                     /label=Hwtx-F
     misc_feature    1241..1942
                     /label=Fc
     CDS             1943..1972
                     /codon_start=1
                     /label=linker
                     /translation="ASGSGGGGGS"
     misc_feature    1972..2083
                     /label=insert from PCR(pcDNA3.1-Hwtx-IV( albumin+matur
                     /label=insert from PCR(pcDNA3.1-Hwtx-IV( albumin+mature 
                     human optimized))
     misc_feature    1973..2083
                     /label=optimized Hwtx-IV(H)
     misc_feature    1984..2043
     primer_bind     complement(2061..2106)
                     /label=oSCL2477
     primer_bind     complement(2061..2083)
                     /label=Hwtx-R
     primer_bind     complement(2064..2083)
                     /label=olyl338
     primer_bind     complement(2065..2086)
                     /label=oSCL2041
     primer_bind     complement(2065..2086)
                     /label=oSCL2475
     primer_bind     complement(2067..2086)
                     /label=oSCL2105
     primer_bind     2086..2121
                     /label=oSCL1795
     polyA_signal    2093..2214
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     2093..2121
                     /label=OXB007
     primer_bind     complement(2104..2123)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2117..2137
                     /label=PSG5-R(SV40-R)
     primer_bind     2158..2177
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2185..2214)
                     /label=OXB016
     primer_bind     complement(2189..2214)
                     /label=OXB008
     primer_bind     complement(2198..2220)
                     /label=oSCL1801
     primer_bind     complement(2198..2214)
                     /label=oSCL1174
     primer_bind     complement(2198..2214)
                     /label=oSCL1796
     misc_feature    2219..2806
                     /label=CMV
     primer_bind     2219..2240
                     /label=oSCL1800
     primer_bind     complement(2775..2806)
                     /label=OXB018
     primer_bind     2782..2806
                     /label=oSCL1791
     primer_bind     2785..2805
                     /label=oSCL1013
     primer_bind     2807..2826
                     /label=T7
     primer_bind     2813..2862
                     /label=oSCL2413
     primer_bind     2836..2862
                     /label=oSCL2381
     CDS             2845..2997
                     /codon_start=1
                     /label=MS2-N55K
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGIAEWISSNSRSQAYKVT
                     CSVRQS"
     CDS             2998..3408
                     /codon_start=1
                     /label=LOV2
                     /translation="LERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGRNCRF
                     LQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQYFIG
                     VQLDGTEHVRDAAEREGVMLIKKTAENIDEAAG"
     primer_bind     3002..3044
                     /label=oSCL2408
     primer_bind     complement(3002..3021)
                     /label=oSCL2407
     CDS             3022..3024
                     /codon_start=1
                     /label=V416I
                     /translation="V"
     primer_bind     3338..3380
                     /label=oSCL2410
     primer_bind     complement(3338..3357)
                     /label=oSCL2409
     CDS             3358..3360
                     /codon_start=1
                     /label=G528A
                     /translation="G"
     primer_bind     3368..3411
                     /label=oSCL2412
     primer_bind     complement(3368..3387)
                     /label=oSCL2411
     CDS             3388..3390
                     /codon_start=1
                     /label=N538E
                     /translation="N"
     CDS             3409..3636
                     /codon_start=1
                     /label=MS2-N55K
                     /translation="QKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATN
                     SDCELIVKAMQGLLKDGNPIPSAIAANSGIY"
     primer_bind     complement(3617..3659)
                     /label=oSCL2415
     primer_bind     complement(3617..3639)
                     /label=oSCL2383
     primer_bind     complement(3617..3636)
                     /label=oSCL2382
     primer_bind     3617..3636
                     /label=oSCL2384
     misc_feature    3646..3694
                     /label=HSV TK poly(A) signal
     primer_bind     complement(3670..3702)
                     /label=oSCL1799
     repeat_region   3703..3843
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3703..3832
                     /label=AAV2 ITR
     rep_origin      3918..4373
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4005..4024)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4215..4236
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4390..4409)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4509..4531
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4569..4587)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4655..4759
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4760..5620
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4978..4997)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5791..6379
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6280..6299
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggcctcta gagttgacat tgattattga ctagttatta atagtaatca
      181 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      241 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      301 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      361 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      421 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      481 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      541 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      601 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      661 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc cttgaaccgg taagaattgc aacaaacaaa tcaccaccgt gaggatcacc
      781 cacggtggtg aaagccttag cctagccgtg aggatcaccc acggtaggca caagaaattc
      841 tacccgtgag gatcacccac gggtagaatc acaaagacta cagccgtgag gatcacccac
      901 ggctgtagac tcaaacttaa aaaggatatc ttcagcaaac tgatcggaga cgagtccgtc
      961 tcaaattgca acaaacaaat caccaccgtg aggatcaccc acggtggtga aagccttagc
     1021 ctagccgtga ggatcaccca cggtaggcac aagaaattct acccgtgagg atcacccacg
     1081 ggtagaatca caaagactac agccgtgagg atcacccacg gctgtagact caaacttaaa
     1141 aaggatatct tcagcaaact gaattcagct tagcgccacc atgtacagga tgcaactcct
     1201 gtcttgcatt gcactaagtc ttgcacttgt cacgaactcg atatcggcca tggttagatc
     1261 tgacaaaact cacacatgcc caccgtgccc agcacctgaa ctcctggggg gaccgtcagt
     1321 cttcctcttc cccccaaaac ccaaggacac cctcatgatc tcccggaccc ctgaggtcac
     1381 atgcgtggtg gtggacgtga gccacgaaga ccctgaggtc aagttcaact ggtacgtgga
     1441 cggcgtggag gtgcataatg ccaagacaaa gccgcgggag gagcagtaca acagcacgta
     1501 ccgtgtggtc agcgtcctca ccgtcctgca ccaggactgg ctgaatggca aggagtacaa
     1561 gtgcaaggtc tccaacaaag ccctcccagc ccccatcgag aaaaccatct ccaaagccaa
     1621 agggcagccc cgagaaccac aggtgtacac cctgccccca tcccgggagg agatgaccaa
     1681 gaaccaggtc agcctgacct gcctggtcaa aggcttctat cccagcgaca tcgccgtgga
     1741 gtgggagagc aatgggcagc cggagaacaa ctacaagacc acgcctcccg tgctggactc
     1801 cgacggctcc ttcttcctct acagcaagct caccgtggac aagagcaggt ggcagcaggg
     1861 gaacgtcttc tcatgctccg tgatgcacga ggctctgcac aaccactaca cgcagaagag
     1921 cctctccctg tctccgggta aagccagcgg aagtggcgga ggaggcggat ccggctgcct
     1981 gggcatcttc aaggcctgca accccagcaa cgaccagtgc tgcaagagca gcaagctcgt
     2041 gtgcagcaga aagacccggt ggtgcaagtg gcagatcggc aagtaactcg agtaagatac
     2101 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     2161 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttacgcgt
     2221 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     2281 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     2341 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2401 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2461 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2521 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2581 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2641 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2701 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2761 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctaat acgactcact
     2821 atagggaagc tttaagccac catggctagc aacttcacac agttcgtgct ggtggacaac
     2881 ggcggcaccg gcgacgtgac cgtggcccct agcaacttcg ccaacggcat cgccgagtgg
     2941 atcagcagca acagcagaag ccaagcctac aaggtgacct gcagcgtgag acagagcctg
     3001 gagagaatcg agaagaactt cgtgatcacc gaccctagac tgcccgacaa ccccatcatc
     3061 ttcgctagcg acagcttcct gcagctgacc gagtacagca gagaggagat cctgggcaga
     3121 aactgcagat tcctgcaagg ccccgagacc gacagagcca ccgtgagaaa gatcagagac
     3181 gccatcgaca atcagaccga ggtgaccgtg cagctgatca actacaccaa gagcggcaag
     3241 aagttctgga acctgttcca cctgcagccc atgagagatc agaagggcga cgtgcagtac
     3301 ttcatcggcg tgcagctgga cggcaccgag cacgtgagag acgccgccga gagagagggc
     3361 gtgatgctga tcaagaagac cgccgagaac atcgacgagg ccgccgggca gaagagaaag
     3421 tacaccatca aagtcgaggt gcccaaggtg gccacccaaa ccgtgggcgg cgtggagctg
     3481 cccgtggccg cctggagaag ctacctgaac atggagctga ccatccccat cttcgccacc
     3541 aacagcgact gcgagctgat cgtgaaggcc atgcaaggcc tgctgaagga cggcaacccc
     3601 attcctagcg ccatcgctgc taacagcggc atttattaac aattgcggca ataaaaagac
     3661 agaataaaac gcacgggtgt tgggtcgttt gttcgcggcc gcaggaaccc ctagtgatgg
     3721 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     3781 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
     3841 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     3901 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     3961 tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt
     4021 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     4081 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     4141 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     4201 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg
     4261 ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct
     4321 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
     4381 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     4441 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     4501 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     4561 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4621 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4681 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4741 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4801 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4861 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4921 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4981 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     5041 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5101 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5161 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5221 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5281 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5341 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5401 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5461 agccggtgag cgtggaagcc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5521 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5581 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5641 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5701 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5761 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5821 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5881 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     5941 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6001 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6061 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6121 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6181 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6241 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6301 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6361 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6421 ctggcctttt gctcacatgt
//