pBAV1K-T5-sfGFP

LOCUS       pBAV1K-T5-sfGFP                3626 bp DNA     circular SYN 15-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 15, 2024 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..3626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..70
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    46..62
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             77..88
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             95..808
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    815..835
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      886..972
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             2303..2314
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(2516..2610)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2626..3420)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEEYRCVKDTEGMSPAKVYKLVGENEILYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEGYED
                     EQSPEKTIELYAECIRLFHSIDISDCPYTNSLDGRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    join(3626,1..21)
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aattcgcggc cgcttctaga ggaaatcata aaaaatttat ttgctttgtg agcggataac
       61 aattataata ctagagaaag aggagaaata ctagatgagc aaaggagaag aacttttcac
      121 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
      181 ccgtggagag ggtgaaggtg atgctacaaa cggaaaactc acccttaaat ttatttgcac
      241 tactggaaaa ctacctgttc cgtggccaac acttgtcact actctgacct atggtgttca
      301 atgcttttcc cgttatccgg atcacatgaa acggcatgac tttttcaaga gtgccatgcc
      361 cgaaggttat gtacaggaac gcactatatc tttcaaagat gacgggacct acaagacgcg
      421 tgctgaagtc aagtttgaag gtgataccct tgttaatcgt atcgagttaa agggtattga
      481 ttttaaagaa gatggaaaca ttcttggaca caaactcgag tacaacttta actcacacaa
      541 tgtatacatc acggcagaca aacaaaagaa tggaatcaaa gctaacttca aaattcgcca
      601 caacgttgaa gatggttccg ttcaactagc agaccattat caacaaaata ctccaattgg
      661 cgatggccct gtccttttac cagacaacca ttacctgtcg acacaatctg tcctttcgaa
      721 agatcccaac gaaaagcgtg accacatggt ccttcttgag tttgtaactg ctgctgggat
      781 tacacatggc atggatgagc tctacaaata gtaatactag tagcggccgc tgcaggcctc
      841 agggcccgat cgatgccgcc gcttaattaa ttaatccaga ggcatcaaat aaaacgaaag
      901 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
      961 agtaggacaa atccgccgcc ctagacctag tgtcatttta tttcccccgt ttcagcatca
     1021 agaacctttg cataacttgc tctatatcca cactgataat tgccctcaaa ccataatcta
     1081 aaggcgctag agtttgttga aacaatatct tttacatcat tcgtatttaa aattccaaac
     1141 tccgctcccc taaggcgaat aaaagccatt aaatcttttg tatttaccaa attatagtca
     1201 tccactatat ctaagagtaa attcttcaat tctctttttt ggctttcatc aagtgttata
     1261 tagcggtcaa tatcaaaatc attaatgttc aaaatatctt ttttgtcgta tatatgttta
     1321 ttcttagcaa tagcgtcctt tgattcatga gtcaaatatt catatgaacc tttgatataa
     1381 tcaagtatct caacatgagc aactgaacta ttccccaatt ttcgcttaat cttgttccta
     1441 acgctttcta ttgttacagg atttcgtgca atatatataa cgtgatagtg tggtttttta
     1501 tagtgctttc catttcgtat aacatcacta ctattccatg tatctttatc ttttttttcg
     1561 tccatatcgt gtaaaggact gacagccata gatacgccca aactctctaa tttttccttc
     1621 caatcattag gaattgagtc aggatataat aaaaatccaa aatttctagc tttagtattt
     1681 ttaatagcca tgatataatt accttatcaa aaacaagtag cgaaaactcg tatccttcta
     1741 aaaacgcgag ctttcgctta ttttttttgt tctgattcct ttcttgcata ttcttctata
     1801 gctaacgccg caaccgcaga ttttgaaaaa cctttttgtt tcgccatatc tgttaatttt
     1861 ttatcttgct cttttgtcag agaaatcata actctttttt tcgattctga aatcaccatt
     1921 taaaaaactc caatcaaata attttataaa gttagtgtat cactttgtaa tcataaaaac
     1981 aacaataaag ctacttaaat atagatttat aaaaaacgtt ggcgaaaacg ttggcgattc
     2041 gttggcgatt gaaaaacccc ttaaaccctt gagccagttg ggatagagcg tttttggcac
     2101 aaaaattggc actcggcact taatgggggg tcgtagtacg gaagcaaaat tcgcttcctt
     2161 tccccccatt tttttccaaa ttccagattt ttttcaaaaa ttttccagcg ctaccgctcg
     2221 gcaaaattgc aagcaatttt taaaatcaaa cccatgaggg aatttcattc cctcatactc
     2281 ccttgagcct cctccaaccg aaatagaagg gcgctgcgct tattatttca ttcagtcatc
     2341 ggctttcata atctaacaga caacatcttc gctgcaaagc cacgctacgc tcaagggctt
     2401 ttacgctacg ataacgcctg ttttaacgat tatgccgata actaaacgaa ataaacgcta
     2461 aaacgtctca gaaacgattt tgagacgttt taataaaaaa tcgcctagtg cttggattct
     2521 caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
     2581 aggtcattac tggatctatc aacgggagtc caagcgagct cggtactaaa acaattcatc
     2641 cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa
     2701 tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat
     2761 gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc
     2821 atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc
     2881 gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc
     2941 ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc
     3001 taagcggccg tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag
     3061 cctgatgcac tccgcataca gctcgatagt cttttcaggg ctttgttcat cttcataccc
     3121 ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg
     3181 ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt
     3241 ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag
     3301 gatttcattt tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt
     3361 tacgcagcgg tattcttcga tcagtttttt caattccggt gatattctca ttttagccat
     3421 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac
     3481 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacag aaaacagcct
     3541 tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac
     3601 cacaattatg atagaatttg acgtcg
//