pCMV-hA3A-BE3-Y132D

LOCUS       pCMV-hA3A-BE3-Y132D                8442 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses hA3A-BE3-Y132D in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-hA3A-BE3-Y132D
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Wang X, Li J, Wang Y, Yang V, Wei J, Wu J, Wang R, Huang X, Chen J, 
            Yang L
  TITLE     Efficient base editing in methylated regions with a human 
            APOBEC3A-Cas9 fusion.
  JOURNAL   Nat Biotechnol.,  2018
REFERENCE   2  (bases 1 to 8442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        162..541
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        542..745
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     696..716
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     787..806
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        787..805
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      813..822
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             819..1415
                     /codon_start=1
                     /gene="human APOBEC3A"
                     /gene_synonym="A3A"
                     /product="human cytidine deaminase (C to U editing enzyme) 
                     that efficiently edits methylated cytosines (Wang et al., 
                     2018)"
                     /label=APOBEC3A
                     /note="also known as hA3A"
                     /translation="MEASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTS
                     VKMDQHRGFLHNQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSW
                     GCAGEVRAFLQENTHVRLRIFAARIYDDDPLYKEALQMLRDAGAQVSIMTYDEFKHCWD
                     TFVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN"
     CDS             1464..5564
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5577..5825
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5838..5858
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5867..5884
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5907..5924)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5913..6137
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6208..6224)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6208..6224)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6221..6243)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6232..6248
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6256..6286)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6301..6322
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6439..6456)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6610..7198)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6690..6709)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7369..8229)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7992..8011
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8230..8334)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8413..8432)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
       61 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
      121 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
      181 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      241 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      301 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      361 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      421 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      481 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      541 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      601 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      661 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      721 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agagatccgc
      781 ggccgctaat acgactcact atagggagag ccgccaccat ggaagccagc ccagcatccg
      841 ggcccagaca cttgatggat ccacacatat tcacttccaa ctttaacaat ggcattggaa
      901 ggcataagac ctacctgtgc tacgaagtgg agcgcctgga caatggcacc tcggtcaaga
      961 tggaccagca caggggcttt ctacacaacc aggctaagaa tcttctctgt ggcttttacg
     1021 gccgccatgc ggagctgcgc ttcttggacc tggttccttc tttgcagttg gacccggccc
     1081 agatctacag ggtcacttgg ttcatctcct ggagcccctg cttctcctgg ggctgtgccg
     1141 gggaagtgcg tgcgttcctt caggagaaca cacacgtgag actgcgtatc ttcgctgccc
     1201 gcatctatga tgatgacccc ctatataagg aggcactgca aatgctgcgg gatgctgggg
     1261 cccaagtctc catcatgacc tacgatgaat ttaagcactg ctgggacacc tttgtggacc
     1321 accagggatg tcccttccag ccctgggatg gactagatga gcacagccaa gccctgagtg
     1381 ggaggctgcg ggccattctc cagaatcagg gaaacagcgg cagcgagact cccgggacct
     1441 cagagtccgc cacacccgaa agtgataaaa agtattctat tggtttagcc atcggcacta
     1501 attccgttgg atgggctgtc ataaccgatg aatacaaagt accttcaaag aaatttaagg
     1561 tgttggggaa cacagaccgt cattcgatta aaaagaatct tatcggtgcc ctcctattcg
     1621 atagtggcga aacggcagag gcgactcgcc tgaaacgaac cgctcggaga aggtatacac
     1681 gtcgcaagaa ccgaatatgt tacttacaag aaatttttag caatgagatg gccaaagttg
     1741 acgattcttt ctttcaccgt ttggaagagt ccttccttgt cgaagaggac aagaaacatg
     1801 aacggcaccc catctttgga aacatagtag atgaggtggc atatcatgaa aagtacccaa
     1861 cgatttatca cctcagaaaa aagctagttg actcaactga taaagcggac ctgaggttaa
     1921 tctacttggc tcttgcccat atgataaagt tccgtgggca ctttctcatt gagggtgatc
     1981 taaatccgga caactcggat gtcgacaaac tgttcatcca gttagtacaa acctataatc
     2041 agttgtttga agagaaccct ataaatgcaa gtggcgtgga tgcgaaggct attcttagcg
     2101 cccgcctctc taaatcccga cggctagaaa acctgatcgc acaattaccc ggagagaaga
     2161 aaaatgggtt gttcggtaac cttatagcgc tctcactagg cctgacacca aattttaagt
     2221 cgaacttcga cttagctgaa gatgccaaat tgcagcttag taaggacacg tacgatgacg
     2281 atctcgacaa tctactggca caaattggag atcagtatgc ggacttattt ttggctgcca
     2341 aaaaccttag cgatgcaatc ctcctatctg acatactgag agttaatact gagattacca
     2401 aggcgccgtt atccgcttca atgatcaaaa ggtacgatga acatcaccaa gacttgacac
     2461 ttctcaaggc cctagtccgt cagcaactgc ctgagaaata taaggaaata ttctttgatc
     2521 agtcgaaaaa cgggtacgca ggttatattg acggcggagc gagtcaagag gaattctaca
     2581 agtttatcaa acccatatta gagaagatgg atgggacgga agagttgctt gtaaaactca
     2641 atcgcgaaga tctactgcga aagcagcgga ctttcgacaa cggtagcatt ccacatcaaa
     2701 tccacttagg cgaattgcat gctatactta gaaggcagga ggatttttat ccgttcctca
     2761 aagacaatcg tgaaaagatt gagaaaatcc taacctttcg cataccttac tatgtgggac
     2821 ccctggcccg agggaactct cggttcgcat ggatgacaag aaagtccgaa gaaacgatta
     2881 ctccatggaa ttttgaggaa gttgtcgata aaggtgcgtc agctcaatcg ttcatcgaga
     2941 ggatgaccaa ctttgacaag aatttaccga acgaaaaagt attgcctaag cacagtttac
     3001 tttacgagta tttcacagtg tacaatgaac tcacgaaagt taagtatgtc actgagggca
     3061 tgcgtaaacc cgcctttcta agcggagaac agaagaaagc aatagtagat ctgttattca
     3121 agaccaaccg caaagtgaca gttaagcaat tgaaagagga ctactttaag aaaattgaat
     3181 gcttcgattc tgtcgagatc tccggggtag aagatcgatt taatgcgtca cttggtacgt
     3241 atcatgacct cctaaagata attaaagata aggacttcct ggataacgaa gagaatgaag
     3301 atatcttaga agatatagtg ttgactctta ccctctttga agatcgggaa atgattgagg
     3361 aaagactaaa aacatacgct cacctgttcg acgataaggt tatgaaacag ttaaagaggc
     3421 gtcgctatac gggctgggga cgattgtcgc ggaaacttat caacgggata agagacaagc
     3481 aaagtggtaa aactattctc gattttctaa agagcgacgg cttcgccaat aggaacttta
     3541 tgcagctgat ccatgatgac tctttaacct tcaaagagga tatacaaaag gcacaggttt
     3601 ccggacaagg ggactcattg cacgaacata ttgcgaatct tgctggttcg ccagccatca
     3661 aaaagggcat actccagaca gtcaaagtag tggatgagct agttaaggtc atgggacgtc
     3721 acaaaccgga aaacattgta atcgagatgg cacgcgaaaa tcaaacgact cagaaggggc
     3781 aaaaaaacag tcgagagcgg atgaagagaa tagaagaggg tattaaagaa ctgggcagcc
     3841 agatcttaaa ggagcatcct gtggaaaata cccaattgca gaacgagaaa ctttacctct
     3901 attacctaca aaatggaagg gacatgtatg ttgatcagga actggacata aaccgtttat
     3961 ctgattacga cgtcgatcac attgtacccc aatccttttt gaaggacgat tcaatcgaca
     4021 ataaagtgct tacacgctcg gataagaacc gagggaaaag tgacaatgtt ccaagcgagg
     4081 aagtcgtaaa gaaaatgaag aactattggc ggcagctcct aaatgcgaaa ctgataacgc
     4141 aaagaaagtt cgataactta actaaagctg agaggggtgg cttgtctgaa cttgacaagg
     4201 ccggatttat taaacgtcag ctcgtggaaa cccgccaaat cacaaagcat gttgcacaga
     4261 tactagattc ccgaatgaat acgaaatacg acgagaacga taagctgatt cgggaagtca
     4321 aagtaatcac tttaaagtca aaattggtgt cggacttcag aaaggatttt caattctata
     4381 aagttaggga gataaataac taccaccatg cgcacgacgc ttatcttaat gccgtcgtag
     4441 ggaccgcact cattaagaaa tacccgaagc tagaaagtga gtttgtgtat ggtgattaca
     4501 aagtttatga cgtccgtaag atgatcgcga aaagcgaaca ggagataggc aaggctacag
     4561 ccaaatactt cttttattct aacattatga atttctttaa gacggaaatc actctggcaa
     4621 acggagagat acgcaaacga cctttaattg aaaccaatgg ggagacaggt gaaatcgtat
     4681 gggataaggg ccgggacttc gcgacggtga gaaaagtttt gtccatgccc caagtcaaca
     4741 tagtaaagaa aactgaggtg cagaccggag ggttttcaaa ggaatcgatt cttccaaaaa
     4801 ggaatagtga taagctcatc gctcgtaaaa aggactggga cccgaaaaag tacggtggct
     4861 tcgatagccc tacagttgcc tattctgtcc tagtagtggc aaaagttgag aagggaaaat
     4921 ccaagaaact gaagtcagtc aaagaattat tggggataac gattatggag cgctcgtctt
     4981 ttgaaaagaa ccccatcgac ttccttgagg cgaaaggtta caaggaagta aaaaaggatc
     5041 tcataattaa actaccaaag tatagtctgt ttgagttaga aaatggccga aaacggatgt
     5101 tggctagcgc cggagagctt caaaagggga acgaactcgc actaccgtct aaatacgtga
     5161 atttcctgta tttagcgtcc cattacgaga agttgaaagg ttcacctgaa gataacgaac
     5221 agaagcaact ttttgttgag cagcacaaac attatctcga cgaaatcata gagcaaattt
     5281 cggaattcag taagagagtc atcctagctg atgccaatct ggacaaagta ttaagcgcat
     5341 acaacaagca cagggataaa cccatacgtg agcaggcgga aaatattatc catttgttta
     5401 ctcttaccaa cctcggcgct ccagccgcat tcaagtattt tgacacaacg atagatcgca
     5461 aacgatacac ttctaccaag gaggtgctag acgcgacact gattcaccaa tccatcacgg
     5521 gattatatga aactcggata gatttgtcac agcttggggg tgactctggt ggttctacta
     5581 atctgtcaga tattattgaa aaggagaccg gtaagcaact ggttatccag gaatccatcc
     5641 tcatgctccc agaggaggtg gaagaagtca ttgggaacaa gccggaaagc gatatactcg
     5701 tgcacaccgc ctacgacgag agcaccgacg agaatgtcat gcttctgact agcgacgccc
     5761 ctgaatacaa gccttgggct ctggtcatac aggatagcaa cggtgagaac aagattaaga
     5821 tgctctctgg tggttctccc aagaagaaga ggaaagtcta accggtcatc atcaccatca
     5881 ccattgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     5941 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     6001 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     6061 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     6121 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga taccgtcgac
     6181 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     6241 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct agggtgccta
     6301 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6361 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6421 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6481 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     6541 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     6601 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6661 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6721 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6781 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6841 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6901 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6961 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7021 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7081 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7141 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7201 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7261 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7321 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7381 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7441 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7501 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7561 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7621 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7681 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7741 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7801 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7861 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7921 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7981 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8041 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8101 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8161 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8221 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8281 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8341 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat cgatctcccg atcccctagg
     8401 gtcgactctc agtacaatct gctctgatgc cgcatagtta ag
//