pBAD-HisB-DpnIR

LOCUS       pBAD-HisB-DpnIR             4728 bp DNA     circular SYN 17-JUN-2026
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pBAD-HisB-mBaoJin2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Replikant
  TITLE     Direct Submission
  JOURNAL   Exported Sep 28, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..4728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     misc_feature    317..1089
                     /label=-1
     terminator      1299..1385
                     /gene="Escherichia coli rrnB"
                     /label=Escherichia coli rrnB terminator
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1477..1504
                     /note="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1523..1614
                     /gene="bla"
                     /label=bla promoter
                     /note="AmpR promoter"
     CDS             1615..2475
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2646..3234
                     /direction=RIGHT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3420..3560
                     /label=bom
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(3824..4702)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /note="araC"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
      301 taacaggagg aattaaccat ggagcttcac tttaacttag aattggttga aacctataaa
      361 tctaattctc aaaaggctcg tattttgacc gaagattggg tctatcgtca gtcatattgc
      421 ccgaactgtg gcaataaccc cttaaaccac tttgaaaaca atcgcccagt cgcggacttc
      481 tactgcaatc attgcagtga agagttcgaa ttaaaatcga aaaaaggtaa ttttagctcc
      541 acgatcaatg atggagcata tgccaccatg atgaaacggg ttcaggcgga caacaatcct
      601 aattttttct ttctgaccta cactaaaaat tttgaagtaa ataacttcct ggtgctgccg
      661 aaacagtttg tgacaccgaa atcgattatc cagcgcaaac cgctcgcgcc gacagcccgt
      721 cgtgcaggtt ggattggctg taacattgat ctgagccaag ttcctagtaa aggccgtatc
      781 tttttggtcc aagacgggca ggtacgcgat ccagagaaag tgactaaaga atttaaacag
      841 ggcctgttcc tgcgcaagtc ctcgctgtca agccgtggtt ggacgatcga aatactgaac
      901 tgtatcgaca agattgaggg tagcgaattt acgctcgaag atatgtatcg attcgaaagt
      961 gatctgaaaa acatcttcgt gaaaaacaac catattaagg aaaaaatacg ccagcagctt
     1021 caaattctgc gcgataaaga gattatcgag ttcaagggcc gggggaaata cagaaagctg
     1081 tgagaattcg aagcttggct gttttggcgg atgagagaag attttcagcc tgatacagat
     1141 taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt
     1201 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
     1261 ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
     1321 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
     1381 caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag
     1441 gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc
     1501 tttttgcgtt tctacaaact cttttgttta tttttctaaa tacattcaaa tatgtatccg
     1561 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     1621 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     1681 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     1741 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     1801 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     1861 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1921 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1981 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     2041 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     2101 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     2161 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     2221 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     2281 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     2341 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     2401 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     2461 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     2521 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     2581 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2641 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2701 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2761 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     2821 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2881 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2941 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3001 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3061 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3121 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3181 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3241 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3301 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3361 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3421 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     3481 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
     3541 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     3601 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     3661 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gcagatcaat tcgcgcgcga
     3721 aggcgaagcg gcatgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     3781 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     3841 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     3901 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     3961 tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct
     4021 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     4081 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     4141 cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt
     4201 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     4261 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     4321 gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa
     4381 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     4441 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     4501 gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     4561 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     4621 cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg
     4681 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagag
//