psiCHECK-2-h2.527425

LOCUS       psiCHECK-2-h2.527425               6616 bp DNA     circular SYN 03-FEB-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6616)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Aug 27, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        62..253
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          489..621
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        666..684
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      688..697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             694..1629
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     misc_feature    1681..2015
                     /label=-10
     polyA_signal    2031..2079
                     /note="synthetic polyadenylation signal"
     promoter        2088..2839
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     regulatory      2869..2878
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2875..4527
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /db_xref="GI:42718124"
                     /protein_id="AAS38485.1"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     RBS             3834..3842
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    4571..4692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4825..4929
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4930..5790
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5961..6549
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca
      481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
      541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
      601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga
      661 gtacttaata cgactcacta taggctagcc accatggctt ccaaggtgta cgaccccgag
      721 caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg
      781 ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt
      841 ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca catcgagccc
      901 gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat
      961 ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga gctgctgaac
     1021 cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc ctttcactac
     1081 tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg
     1141 atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat caagagcgaa
     1201 gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct cccaagcaag
     1261 atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt caaggagaag
     1321 ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt taagggaggc
     1381 aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc cagcgacgat
     1441 ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat tgtcgaggga
     1501 gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt cagccaggag
     1561 gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt gctgaagaac
     1621 gagcagtaat tctaggcgat cgctcgagcc cgggaattcg tttaaaccta gagcggccgc
     1681 aaaccaaagg attttaaaat actgagtaca aacctgcata tgaggtttgt aagacaatgg
     1741 gtagaatact ggatgtcaca actaaaaatg aggccagtat cacggtaatt ttgcaagatg
     1801 tgtgtcaaat atcaatccat tgttgaaagg tgtccaattc atatataccc taaaccttag
     1861 taaaaaatgt gccacatcca caccacatga acagcacata tcatcacaaa aatagtgttc
     1921 tttgaagaat gcgtaggtac cactagacac ctattattaa tcattgtttc tccaaagaag
     1981 gaagagttta gatatggttt tgggagagta tcttagcggc cgctggccgc aataaaatat
     2041 ctttattttc attacatctg tgtgttggtt ttttgtgtga ggatctaaat gagtcttcgg
     2101 acctcgcggg ggccgcttaa gcggtggtta gggtttgtct gacgcggggg gagggggaag
     2161 gaacgaaaca ctctcattcg gaggcggctc ggggtttggt cttggtggcc acgggcacgc
     2221 agaagagcgc cgcgatcctc ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt
     2281 cggcgggtgg taactggcgg gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc
     2341 ttttcggtct gctcgcagac ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg
     2401 tcctaggctc catggggacc gtatacgtgg acaggctctg gagcatccgc acgactgcgg
     2461 tgatattacc ggagaccttc tgcgggacga gccgggtcac gcggctgacg cggagcgtcc
     2521 gttgggcgac aaacaccagg acggggcaca ggtacactat cttgtcaccc ggaggcgcga
     2581 gggactgcag gagcttcagg gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc
     2641 gtttgctggc ggtgtccccg gaagaaatat atttgcatgt ctttagttct atgatgacac
     2701 aaaccccgcc cagcgtcttg tcattggcga attcgaacac gcagatgcag tcggggcggc
     2761 gcggtcccag gtccacttcg catattaagg tgacgcgtgt ggcctcgaac accgagcgac
     2821 cctgcagcga cccgcttaaa agcttggcat tccggtactg ttggtaaagc caccatggcc
     2881 gatgctaaga acattaagaa gggccctgct cccttctacc ctctggagga tggcaccgct
     2941 ggcgagcagc tgcacaaggc catgaagagg tatgccctgg tgcctggcac cattgccttc
     3001 accgatgccc acattgaggt ggacatcacc tatgccgagt acttcgagat gtctgtgcgc
     3061 ctggccgagg ccatgaagag gtacggcctg aacaccaacc accgcatcgt ggtgtgctct
     3121 gagaactctc tgcagttctt catgccagtg ctgggcgccc tgttcatcgg agtggccgtg
     3181 gcccctgcta acgacattta caacgagcgc gagctgctga acagcatggg catttctcag
     3241 cctaccgtgg tgttcgtgtc taagaagggc ctgcagaaga tcctgaacgt gcagaagaag
     3301 ctgcctatca tccagaagat catcatcatg gactctaaga ccgactacca gggcttccag
     3361 agcatgtaca cattcgtgac atctcatctg cctcctggct tcaacgagta cgacttcgtg
     3421 ccagagtctt tcgacaggga caaaaccatt gccctgatca tgaacagctc tgggtctacc
     3481 ggcctgccta agggcgtggc cctgcctcat cgcaccgcct gtgtgcgctt ctctcacgcc
     3541 cgcgacccta ttttcggcaa ccagatcatc cccgacaccg ctattctgag cgtggtgcca
     3601 ttccaccacg gcttcggcat gttcaccacc ctgggctacc tgatttgcgg ctttcgggtg
     3661 gtgctgatgt accgcttcga ggaggagctg ttcctgcgca gcctgcaaga ctacaaaatt
     3721 cagtctgccc tgctggtgcc aaccctgttc agcttcttcg ctaagagcac cctgatcgac
     3781 aagtacgacc tgtctaacct gcacgagatt gcctctggcg gcgccccact gtctaaggag
     3841 gtgggcgaag ccgtggccaa gcgctttcat ctgccaggca tccgccaggg ctacggcctg
     3901 accgagacaa ccagcgccat tctgattacc ccagagggcg acgacaagcc tggcgccgtg
     3961 ggcaaggtgg tgccattctt cgaggccaag gtggtggacc tggacaccgg caagaccctg
     4021 ggagtgaacc agcgcggcga gctgtgtgtg cgcggcccta tgattatgtc cggctacgtg
     4081 aataaccctg aggccacaaa cgccctgatc gacaaggacg gctggctgca ctctggcgac
     4141 attgcctact gggacgagga cgagcacttc ttcatcgtgg accgcctgaa gtctctgatc
     4201 aagtacaagg gctaccaggt ggccccagcc gagctggagt ctatcctgct gcagcaccct
     4261 aacattttcg acgccggagt ggccggcctg cccgacgacg atgccggcga gctgcctgcc
     4321 gccgtcgtcg tgctggaaca cggcaagacc atgaccgaga aggagatcgt ggactatgtg
     4381 gccagccagg tgacaaccgc caagaagctg cgcggcggag tggtgttcgt ggacgaggtg
     4441 cccaagggcc tgaccggcaa gctggacgcc cgcaagatcc gcgagatcct gatcaaggct
     4501 aagaaaggcg gcaagatcgc cgtgtaataa ttctagagtc ggggcggccg gccgcttcga
     4561 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     4621 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     4681 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     4741 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatcca
     4801 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4861 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4921 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     4981 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     5041 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5101 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5161 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5221 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5281 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5341 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5401 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5461 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     5521 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     5581 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     5641 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     5701 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5761 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     5821 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5881 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5941 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6001 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6061 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     6121 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6181 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6241 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6301 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6361 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6421 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6481 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6541 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6601 gctcacatgg ctcgac
//