pAAVLINK-EF1alpha-HA-5'-KIF13B

LOCUS       pAAVLINK-EF1alpha-HA-5'-KIF13B                6683 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6683)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        158..700
                     /label=EF1-alpha
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        187..398
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             411..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    505..510
                     /label=SDS
     CDS             716..742
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             749..3823
                     /codon_start=1
                     /gene="KIF13B"
                     /gene_synonym="GAKIN"
                     /product="kinesin-like protein KIF13B"
                     /label=KIF13B
                     /note="kinesin 13B; guanylate kinase associated kinesin; 
                     kinesin-like protein GAKIN"
                     /db_xref="GI:46852172"
                     /db_xref="CCDS:CCDS55217.1"
                     /db_xref="GeneID:23303"
                     /db_xref="HGNC:HGNC:14405"
                     /db_xref="MIM:607350"
                     /protein_id="NP_056069.2"
                     /translation="MGDSKVKVAVRIRPMNRRETDLHTKCVVDVDANKVILNPVNTNLS
                     KGDARGQPKVFAYDHCFWSMDESVKEKYAGQDIVFKCLGENILQNAFDGYNACIFAYGQ
                     TGSGKSYTMMGTADQPGLIPRLCSGLFERTQKEENEEQSFKVEVSYMEIYNEKVRDLLD
                     PKGSRQTLKVREHSVLGPYVDGLSKLAVTSYKDIESLMSEGNKSRTVAATNMNEESSRS
                     HAVFKITLTHTLYDVKSGTSGEKVGKLSLVDLAGSERATKTGAAGDRLKEGSNINKSLT
                     TLGLVISALADQSAGKNKNKFVPYRDSVLTWLLKDSLGGNSKTAMVATVSPAADNYDET
                     LSTLRYADRAKHIVNHAVVNEDPNARIIRDLREEVEKLREQLTKAEAMKSPELKDRLEE
                     SEKLIQEMTVTWEEKLRKTEEIAQERQKQLESLGISLQSSGIKVGDDKCFLVNLNADPA
                     LNELLVYYLKEHTLIGSANSQDIQLCGMGILPEHCIIDITSEGQVMLTPQKNTRTFVNG
                     SSVSSPIQLHHGDRILWGNNHFFRLNLPKKKKKAEREDEDQDPSMKNENSSEQLDVDGD
                     SSSEVSSEVNFNYEYAQMEVTMKALGSNDPMQSILNSLEQQHEEEKRSALERQRLMYEH
                     ELEQLRRRLSPEKQNCRSMDRFSFHSPSAQQRLRQWAEEREATLNNSLMRLREQIVKAN
                     LLVREANYIAEELDKRTEYKVTLQIPASSLDANRKRGSLLSEPAIQVRRKGKGKQIWSL
                     EKLDNRLLDMRDLYQEWKECEEDNPVIRSYFKRADPFYDEQENHSLIGVANVFLESLFY
                     DVKLQYAVPIINQKGEVAGRLHVEVMRLSGDVGERIAGGDEVAEVSFEKETQENKLVCM
                     VKILQATGLPQHLSHFVFCKYSFWDQQEPVIVAPEVDTSSSSVSKEPHCMVVFDHCNEF
                     SVNITEDFIEHLSEGALAIEVYGHKINDPRKNPALWDLGIIQAKTRSLRDRWSEVTRKL
                     EFWVQILEQNENGEYCPVEVISAKDVPTGGIFQLRQ"
     regulatory      1722..1731
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3295..3300
                     /label=SDS
     misc_feature    3474..3479
                     /label=SDS
     misc_feature    3477..3482
                     /label=SDS
     misc_feature    3799..3804
                     /label=SDS
     intron          3824..3864
                     /label=Splicing Donor
     misc_feature    3868..3901
                     /label=lox2272-JT1571
     repeat_region   3946..4086
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3946..4075
                     /label=AAV2 ITR
     repeat_region   3947..4087
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4161..4616
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4898..5002
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5003..5863
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6034..6622
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
      421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
      721 ttatgacgta cctgactatg ctggatctat gggggactcc aaagtgaaag tggcggtgcg
      781 gatacgaccc atgaaccggc gagagactga cttgcatacc aaatgtgtgg tggatgtgga
      841 tgcaaacaag gttattctta atcctgtaaa tacgaatctt tccaaaggag atgcccgggg
      901 ccagccgaag gtgtttgctt atgatcattg tttctggtct atggatgaat ctgtcaaaga
      961 aaagtatgca ggtcaagata ttgttttcaa gtgccttgga gagaatatcc tgcagaatgc
     1021 ttttgatggc tacaatgcat gtatctttgc ctatggacag actggctctg gaaaatctta
     1081 taccatgatg ggcacagctg accaacctgg attaatccca agactttgca gtggactctt
     1141 tgaacgaact cagaaagagg aaaatgaaga acagagtttt aaagtagaag tgtcctacat
     1201 ggaaatttat aatgaaaaag ttcgagacct tcttgatccc aaaggaagcc gtcagacgtt
     1261 gaaagtcaga gagcatagtg tgttgggacc ttatgtcgac ggactttcta aactggctgt
     1321 cacaagctac aaggatattg agtcgttgat gtctgagggt aacaaatctc gcacagttgc
     1381 tgcaaccaac atgaacgagg agagtagccg atcccatgca gttttcaaaa tcaccctcac
     1441 acatactctc tacgatgtga agtctgggac atctggagag aaagtgggca aactcagcct
     1501 ggtggattta gctggcagtg aacgagcaac gaagacaggc gctgcagggg acaggctgaa
     1561 ggaagggagc aacattaaca agtccctcac aaccctcggt ctggttatct cagctcttgc
     1621 agatcagagt gctggcaaaa acaagaataa atttgttcca tatcgtgact cagttctcac
     1681 ttggctgctc aaagacagcc tcgggggtaa cagcaagacc gccatggtgg ctactgtgag
     1741 tcctgcagct gataactatg atgaaaccct ctcaactctg cggtatgcag atcgagccaa
     1801 gcacattgta aaccacgctg tggtgaatga ggaccctaat gcccgaatta tccgggatct
     1861 ccgggaagaa gttgagaaac tccgggagca gctgaccaaa gcagaggcaa tgaaatctcc
     1921 agagctaaag gaccggctgg aagaatctga gaagctaatc caggaaatga ctgtgacctg
     1981 ggaggagaaa ttaaggaaaa cggaggagat tgcacaggaa cgacagaaac agcttgagag
     2041 tcttggaata tctcttcagt cttcgggaat caaagttggg gatgataaat gcttccttgt
     2101 gaatctgaat gctgacccag ctctgaatga gcttctggtg tactatttaa aggaacatac
     2161 attgataggg tcagcaaatt cccaagatat ccaactgtgc ggcatgggaa ttcttcctga
     2221 acactgtatt atagacatca cgtcagaagg ccaggttatg ctgactcctc agaagaacac
     2281 cagaacattt gtaaatgggt catctgtctc cagtccaata cagctacacc atggggacag
     2341 gatattatgg ggaaacaatc atttcttcag actcaatttg cctaaaaaga aaaagaaagc
     2401 agaacgagag gatgaggacc aggatccctc catgaagaac gagaatagtt ctgagcagct
     2461 ggatgtagac ggagactcct ccagcgaggt gtccagtgaa gttaacttta attacgaata
     2521 cgcacagatg gaggtcacca tgaaggccct gggcagcaat gatccgatgc agtccatatt
     2581 aaacagccta gaacaacagc atgaagaaga aaaacgatct gcactggagc gccagaggct
     2641 tatgtatgag cacgaattgg agcagctccg gagaaggctg tctcctgaga agcagaactg
     2701 ccggagcatg gacaggtttt ctttccactc gcccagcgct cagcaacgct taagacagtg
     2761 ggctgaggag agagaagcaa cgttgaataa cagcctgatg aggctgaggg aacaaattgt
     2821 taaggccaat ctattggtga gagaagctaa ttacattgct gaggagctgg ataaaagaac
     2881 agaatacaaa gttaccctac agattccagc ctccagcctg gatgccaaca ggaagcgagg
     2941 ctctcttctt agtgagcctg caatccaggt gagaagaaaa ggaaaaggaa agcagatttg
     3001 gtctttggaa aaactggaca acaggctgtt ggatatgaga gacctttatc aggagtggaa
     3061 agagtgtgaa gaagataacc cagtaatacg atcatacttc aaacgtgctg atccattcta
     3121 tgatgagcag gaaaatcaca gtctcattgg ggtggccaat gtcttcctcg agtcactttt
     3181 ctatgatgtg aagttacaat acgctgttcc catcatcaac cagaaaggag aggtggcagg
     3241 tcggctgcac gtggaggtga tgcgactcag tggtgatgtt ggggagagga tcgcaggagg
     3301 cgatgaggtg gcagaggtct cctttgagaa ggagacccag gagaacaaac tggtgtgcat
     3361 ggttaaaatc ctgcaagcta ctgggttgcc acagcatctg tcccactttg tgttctgcaa
     3421 atacagcttc tgggatcaac aggagccggt gattgtcgct cctgaagtgg acacctcctc
     3481 ctcttccgtc agcaaggagc cgcactgcat ggttgtcttt gatcattgca atgagttttc
     3541 tgttaacatc accgaagact ttatcgagca tctttccgaa ggagcattgg caattgaagt
     3601 atatggacat aaaataaacg atccccggaa aaaccccgcc ctgtgggatt tgggaatcat
     3661 ccaagcaaag acacgtagtc ttcgggacag atggagtgaa gtgaccagga aattggaatt
     3721 ctgggttcaa atcttggaac agaatgagaa tggtgaatac tgccctgtag aagtgatttc
     3781 tgcaaaggat gtcccaacag gaggaatctt ccagctccgg caggtaagta tcaaggttac
     3841 aagacaggtt taaggagacc aataagctat tattcgtata ggatacttta tacgaagtta
     3901 ttaagttatg gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga
     3961 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
     4021 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
     4081 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     4141 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     4201 ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt
     4261 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     4321 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
     4381 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     4441 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc
     4501 gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga
     4561 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
     4621 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     4681 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     4741 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     4801 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     4861 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     4921 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     4981 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     5041 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     5101 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     5161 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     5221 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     5281 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     5341 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     5401 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     5461 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     5521 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     5581 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     5641 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     5701 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     5761 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     5821 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     5881 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     5941 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     6001 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     6061 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6121 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6181 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     6241 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6301 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6361 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6421 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6481 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6541 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6601 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6661 ttgctggcct tttgctcaca tgt
//