| 质粒名称: | pBAD-6xHis-miRFP670-2-HO1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000097 |
| 产品用途: | Expresses miRFP670-2 with Heme Oxygenase 1 (HO1) in bacteria for purification |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pBAD |
| 表达系统: | 细菌表达 |
| 启动子: | araBAD |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | miRFP670-2, HO1 |
| 物种来源: | Synthetic, Homo sapiens |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Quantitative assessment of near-infrared fluorescent proteins
Zhang H., Papadaki S., Sun X., Wang X., Drobizhev M., Yao L., Rehbock M., Köster R.W., Wu L., Namikawa K., Piatkevich K.D.
Nat. Methods 2023 2010 20, 1605–1616 (2023)
|
买家导航
pBAD-6xHis-miRFP670-2-HO1载体质粒基本信息
pBAD-6xHis-miRFP670-2-HO1质粒图谱载体图谱和pBAD-6xHis-miRFP670-2-HO1载体序列质粒序列多克隆位点信息
pBAD-6xHis-miRFP670-2-HO1质粒载体简介
pBAD-6xHis-miRFP670-2-HO1质粒序列载体序列
LOCUS pBAD-6xHis-miRFP670-2-HO1 5688 bp DNA circular SYN 21-APR-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5688)
AUTHORS Kiryl Piatkevich
TITLE Direct Submission
JOURNAL Exported Apr 14, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5688
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 120..285
/gene="araBAD"
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction"
CDS 319..327
/codon_start=1
/label=Start
/translation="MGS"
primer_bind 324..358
/label=miRFP670-2-F
CDS 328..345
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 355..1287
/codon_start=1
/product="near-infrared fluorescent protein with an
emission peak at 670 nm, engineered from a bacterial
phytochrome (Shcherbakova and Verkhusha, 2013)"
/label=miRFP670-2
/note="derived from Rhodopseudomonas palustris RpBphP6"
/translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
PRAVPMAQRVAAKRFAERLSTHFTAAHHQR"
regulatory 355..358
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
primer_bind 1268..1320
/label=0816-HO1-F
primer_bind complement(1268..1287)
/label=0816-PM345-R
misc_feature 1291..2043
/label=to be cloned into PM283
CDS 1321..2043
/codon_start=1
/label=HO1
/translation="MSVNLASQLREGTKKSHSMAENVGFVKCFLKGVVEKNSYRKLVGN
LYFVYSAMEEEMAKFKDHPILSHIYFPELNRKQSLEQDLQFYYGSNWRQEVKISAAGQA
YVDRVRQVAATAPELLVAHSYTRYLGDLSGGQILKKIAQNAMNLHDGGTAFYEFADIDD
EKAFKNTYRQAMNDLPIDQATAERIVDEANDAFAMNMKMFNELEGNLIKAIGIMVFNSL
TRRRSQGSTEVGLATSEG"
primer_bind 1595..1619
/label=HO1-F
primer_bind complement(1754..1775)
/label=HO1-R
primer_bind complement(2038..2074)
/label=miRFP670-2-R
terminator 2259..2345
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 2437..2464
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 2483..2574
/gene="bla"
/label=AmpR promoter
CDS 2575..3435
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3606..4194
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 4380..4520
/label=bom
/note="basis of mobility region from pBR322"
CDS complement(4784..5662)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/label=araC
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
ORIGIN
1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
301 taacaggagg aattaaccat ggggagccac catcaccatc accatggcct cgagatggcg
361 cgtaaggtcg atctcacctc ctgcgatcgc gagccgatcc acatccccgg cagcattcag
421 ccgtgcggct gcctgctagc ctgcgacgcg caggcggtgc ggatcacgcg cattacggaa
481 aatgccggcg cgttctttgg acgcgaaact ccgcgggtcg gtgagctact cgccgattac
541 ttcggcgaga ccgaagccca tgcgctgcgc aacgcactgg cgcagtcctc cgatccaaag
601 cgaccggcgc tgatcttcgg ttggcgcgac ggcctgaccg gccgcacctt cgacatctca
661 ctgcatcgcc atgacggtac atcgatcatc gagttcgagc ctgcggcggc cgaacaggcc
721 gacaatccgc tgcggctgac gcggcagatc atcgcgcgca ccaaagaact gaagtcgctc
781 gaagagatgg ccgcacgggt gccgcgctat ctgcaggcga tgctcggcta tcaccgcgtg
841 atgttgtacc gcttcgcgga cgacggctcc gggatggtga tcggcgaggc gaagcgcagc
901 gacctcgaga gctttctcgg tcagcacttt ccggcgtcgc tggtcccgca gcaggcgcgg
961 ctactgtact tgaagaacgc gatccgcgtg gtctcggatt cgcgcggcat cagcagccgg
1021 atcgtgcccg agcacgacgc ctccggcgcc gcgctcgatc tgtcgttcgc gcacctgcgc
1081 agcatctcgc cctgccatct cgaatttctg cggaacatgg gcgtcagcgc ctcgatgtcg
1141 ctgtcgatca tcattgacgg cacgctatgg ggattgatca tctgtcatca ttacgagccg
1201 cgtgccgtgc cgatggcgca gcgcgtcgcg gccaaaaggt tcgccgagcg cttatcgacg
1261 cacttcaccg ccgcccacca ccaacgctaa tagctgcaga agaaggagat atacataccc
1321 atgagtgtca acttagcttc ccagttgcgg gaagggacga aaaaatccca ctccatggcg
1381 gagaacgtcg gctttgtcaa atgcttcctc aagggcgttg tcgagaaaaa ttcctaccgt
1441 aagctggttg gcaatctcta ctttgtctac agtgccatgg aagaggaaat ggcaaaattt
1501 aaggaccatc ccatcctcag ccacatttac ttccccgaac tcaaccgcaa acaaagccta
1561 gagcaagacc tgcaattcta ttacggctcc aactggcggc aagaagtgaa aatttctgcc
1621 gctggccaag cctatgtgga ccgagtccgg caagtggccg ctacggcccc tgaattgttg
1681 gtggcccatt cctacacccg ttacctgggg gatctttccg gcggtcaaat tctcaagaaa
1741 attgcccaaa atgccatgaa tctccacgat ggtggcacag ctttctatga atttgccgac
1801 attgatgacg aaaaggcttt taaaaatacc taccgtcaag ctatgaatga tctgcccatt
1861 gaccaagcca ccgccgaacg gattgtggat gaagccaatg acgcctttgc catgaacatg
1921 aaaatgttca acgaacttga aggcaacctg atcaaggcga tcggcattat ggtgttcaac
1981 agcctcaccc gtcgccgcag tcaaggcagc accgaagttg gcctcgccac ctccgaaggc
2041 taagaattcg aagcttggct gttttggcgg atgagagaag attttcagcc tgatacagat
2101 taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt
2161 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
2221 ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
2281 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
2341 caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag
2401 gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc
2461 tttttgcgtt tctacaaact cttttgttta tttttctaaa tacattcaaa tatgtatccg
2521 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
2581 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
2641 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
2701 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
2761 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
2821 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
2881 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
2941 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
3001 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
3061 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
3121 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
3181 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
3241 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
3301 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
3361 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
3421 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
3481 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
3541 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
3601 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
3661 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
3721 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
3781 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
3841 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
3901 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
3961 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
4021 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
4081 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
4141 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
4201 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
4261 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
4321 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
4381 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
4441 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
4501 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
4561 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
4621 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gcagatcaat tcgcgcgcga
4681 aggcgaagcg gcatgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
4741 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
4801 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
4861 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
4921 tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct
4981 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
5041 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
5101 cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt
5161 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
5221 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
5281 gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa
5341 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
5401 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
5461 gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat
5521 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
5581 cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg
5641 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagag
//
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