pDJ72

LOCUS       pDJ72               5104 bp DNA     circular SYN 08-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Aug 5, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    6..100
                     /label=important segment
     misc_feature    6..15
                     /label=SRE-1
                     /note="sterol regulatory element-1"
     misc_feature    27..36
                     /label=SRE-1
                     /note="sterol regulatory element-1"
     misc_feature    70..78
                     /label=E-box
     misc_feature    92..100
                     /label=E-box
     misc_feature    108..255
                     /label=CMVmin
     promoter        130..167
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     236..256
                     /label=181-WKG-593-F
     misc_feature    299..1818
                     /label=important segment
     CDS             299..1801
                     /codon_start=1
                     /product="secreted alkaline phosphatase from human 
                     placenta"
                     /label=SEAP
                     /note="The natural enzyme has a C-terminal transmembrane 
                     sequence that is replaced by a GPI anchor."
                     /translation="MLLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQP
                     AQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDK
                     HVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVV
                     TTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRK
                     YMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTH
                     LMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESR
                     AYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKA
                     RDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFA
                     RGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     primer_bind     complement(347..365)
                     /label=175-WKG-587-R
     primer_bind     792..811
                     /label=391-1151-F
     misc_feature    1054..1059
                     /label=putative TEAD-binding sites
     primer_bind     1177..1195
                     /label=392-1151-F2
     primer_bind     1790..1814
                     /label=183-WKG-599-F
     polyA_signal    2861..2995
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     2885..2904
                     /label=58-SV40poly(A)-Rseq
     rep_origin      complement(3357..3945)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3444..3468)
                     /label=225-WKG-1079-F
     primer_bind     3841..3869
                     /label=197-WKG-623-F3
     CDS             complement(4412..4790)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     4460..4482
                     /label=198-WKG-623-F4
     promoter        complement(4975..5079)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtatcac cccacacatc tgacgtatca ccccacacat ctgacgtcag gatgtcctgc
       61 aggacatcta tcacgtgact gcaggacatc tatcacgtga ctgcaggtcg agctcggtac
      121 ccgggtcgag taggcgtgta cggtgggagg cctatataag cagagctcgt ttagtgaacc
      181 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc
      241 gatccagcct ccgcggaatt cgagctcgcc cggggatcct ctagagtcag cttcctgcat
      301 gctgctgctg ctgctgctgc tgggcctgag gctacagctc tccctgggca tcatcccagt
      361 tgaggaggag aacccggact tctggaaccg cgaggcagcc gaggccctgg gtgccgccaa
      421 gaagctgcag cctgcacaga cagccgccaa gaacctcatc atcttcctgg gcgatgggat
      481 gggggtgtct acggtgacag ctgccaggat cctaaaaggg cagaagaagg acaaactggg
      541 gcctgagata cccctggcca tggaccgctt cccatatgtg gctctgtcca agacatacaa
      601 tgtagacaaa catgtgccag acagtggagc cacagccacg gcctacctgt gcggggtcaa
      661 gggcaacttc cagaccattg gcttgagtgc agccgcccgc tttaaccagt gcaacacgac
      721 acgcggcaac gaggtcatct ccgtgatgaa tcgggccaag aaagcaggga agtcagtggg
      781 agtggtaacc accacacgag tgcagcacgc ctcgccagcc ggcacctacg cccacacggt
      841 gaaccgcaac tggtactcgg acgccgacgt gcctgcctcg gcccgccagg aggggtgcca
      901 ggacatcgct acgcagctca tctccaacat ggacattgac gtgatcctag gtggaggccg
      961 aaagtacatg tttcgcatgg gaaccccaga ccctgagtac ccagatgact acagccaagg
     1021 tgggaccagg ctggacggga agaatctggt gcaggaatgg ctggcgaagc gccagggtgc
     1081 ccggtatgtg tggaaccgca ctgagctcat gcaggcttcc ctggacccgt ctgtgaccca
     1141 tctcatgggt ctctttgagc ctggagacat gaaatacgag atccaccgag actccacact
     1201 ggacccctcc ctgatggaga tgacagaggc tgccctgcgc ctgctgagca ggaacccccg
     1261 cggcttcttc ctcttcgtgg agggtggtcg catcgaccat ggtcatcatg aaagcagggc
     1321 ttaccgggca ctgactgaga cgatcatgtt cgacgacgcc attgagaggg cgggccagct
     1381 caccagcgag gaggacacgc tgagcctcgt cactgccgac cactcccacg tcttctcctt
     1441 cggaggctac cccctgcgag ggagctccat cttcgggctg gcccctggca aggcccggga
     1501 caggaaggcc tacacggtcc tcctatacgg aaacggtcca ggctatgtgc tcaaggacgg
     1561 cgcccggccg gatgttaccg agagcgagag cgggagcccc gagtatcggc agcagtcagc
     1621 agtgcccctg gacgaagaga cccacgcagg cgaggacgtg gcggtgttcg cgcgcggccc
     1681 gcaggcgcac ctggttcacg gcgtgcagga gcagaccttc atagcgcacg tcatggcctt
     1741 cgccgcctgc ctggagccct acaccgcctg cgacctggcg ccccccgccg gcaccaccga
     1801 cgccgcgcac ccgggttaac ccgtggtccc cgcgttgctt cctctgctgg ccgggaccct
     1861 gctgctgctg gagacggcca ctgctccctg agtgtcccgt ccctggggct cctgcttccc
     1921 catcccggag ttctcctgct ccccacctcc tgtcgtcctg cctggcctcc agcccgagtc
     1981 gtcatccccg gagtccctat acagaggtcc tgccatggaa ccttcccctc cccgtgcgct
     2041 ctggggactg agcccatgac accaaacctg ccccttggct gctctcggac tccctacccc
     2101 aaccccaggg actgcaggtt gtgccctgtg gctgcctgca ccccaggaaa ggagggggct
     2161 caggccatcc agccaccacc tacagcccag tggcctcgag ctgcagaagc ttttaaaaca
     2221 gctctggggt tgtacccacc ccagaggccc acgtggcggc tagtactccg gtattgcggt
     2281 acccttgtac gcctgtttta tactcccttc ccgtaactta gacgcacaaa accaagttca
     2341 atagaagggg gtacaaacca gtaccaccac gaacaagcac ttctgtttcc ccggtgatgt
     2401 cgtatagact gcttgcgtgg ttgaaagcga cggatccgtt atccgcttat gtacttcgag
     2461 aagcccagta ccacctcgga atcttcgatg cgttgcgctc agcactcaac cccagagtgt
     2521 agcttaggct gatgagtctg gacatccctc accggtgacg gtggtccagg ctgcgttggc
     2581 ggcctaccta tggctaacgc catgggacgc tagttgtgaa caaggtgtga agagcctatt
     2641 gagctacata agaatcctcc ggcccctgaa tgcggctaat cccaacctcg gagcaggtgg
     2701 tcacaaacca gtgattggcc tgtcgtaacg cgcaagtccg tggcggaacc gactactttg
     2761 ggtgtccgtg tttcctttta ttttattgtg gctgcttatg gtgacaatca cagattgtta
     2821 tcataaagcg aattggattg cggccgctta agctagcttc gatccagaca tgataagata
     2881 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     2941 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     3001 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     3061 caagtaaaac ctctacaaat gtggtatggc tgattatgat cctgcctcgc gcgtttcggt
     3121 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     3181 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     3241 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     3301 catcagagca gattgtactg agagtgcacc atatgtcggg ccgcgttgct ggcgtttttc
     3361 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     3421 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     3481 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     3541 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     3601 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     3661 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     3721 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     3781 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     3841 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     3901 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     3961 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     4021 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     4081 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     4141 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     4201 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     4261 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     4321 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     4381 agagtaagta gttcgccagt taatagtgcg caacgttgtt gccattgcta caggcatcgt
     4441 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     4501 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     4561 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     4621 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     4681 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     4741 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     4801 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     4861 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     4921 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     4981 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     5041 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     5101 acct
//