psiCHECK-2-h1-cluster18

LOCUS       psiCHECK-2-h1-cluster18             6605 bp DNA     circular SYN 16-MAY-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6605)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Sep 15, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        62..253
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          489..621
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        666..684
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      688..697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             694..1629
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     misc_feature    1681..2004
                     /label=h1-cluster18
     polyA_signal    2020..2068
                     /note="synthetic polyadenylation signal"
     promoter        2077..2828
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     regulatory      2858..2867
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2864..4516
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /db_xref="GI:42718124"
                     /protein_id="AAS38485.1"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     RBS             3823..3831
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    4560..4681
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4814..4918
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4919..5779
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5950..6538
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca
      481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
      541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
      601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga
      661 gtacttaata cgactcacta taggctagcc accatggctt ccaaggtgta cgaccccgag
      721 caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg
      781 ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt
      841 ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca catcgagccc
      901 gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat
      961 ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga gctgctgaac
     1021 cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc ctttcactac
     1081 tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg
     1141 atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat caagagcgaa
     1201 gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct cccaagcaag
     1261 atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt caaggagaag
     1321 ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt taagggaggc
     1381 aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc cagcgacgat
     1441 ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat tgtcgaggga
     1501 gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt cagccaggag
     1561 gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt gctgaagaac
     1621 gagcagtaat tctaggcgat cgctcgagcc cgggaattcg tttaaaccta gagcggccgc
     1681 ataggatatt ctaaatctga gctgtcattt aaattatgtt ttaataatgt ggctcatagg
     1741 tacaaggaat ttgattaatc agaattggaa agaaaaaatt gtgtagctta acttgtacat
     1801 ctttgtttcc ctgaaatcta ctcaccatgc cacagccaaa aatgatgttt caaaaattca
     1861 ggtctgatca agtagaattg cccacccggc ttaacaattt ttaatgactt cctttttcat
     1921 tttattgtgg taagaagagt taacacaata tctctagaat taattcatct tcataaactg
     1981 aaacttcata ccaattgaac aactgcggcc gctggccgca ataaaatatc tttattttca
     2041 ttacatctgt gtgttggttt tttgtgtgag gatctaaatg agtcttcgga cctcgcgggg
     2101 gccgcttaag cggtggttag ggtttgtctg acgcgggggg agggggaagg aacgaaacac
     2161 tctcattcgg aggcggctcg gggtttggtc ttggtggcca cgggcacgca gaagagcgcc
     2221 gcgatcctct taagcacccc cccgccctcc gtggaggcgg gggtttggtc ggcgggtggt
     2281 aactggcggg ccgctgactc gggcgggtcg cgcgccccag agtgtgacct tttcggtctg
     2341 ctcgcagacc cccgggcggc gccgccgcgg cggcgacggg ctcgctgggt cctaggctcc
     2401 atggggaccg tatacgtgga caggctctgg agcatccgca cgactgcggt gatattaccg
     2461 gagaccttct gcgggacgag ccgggtcacg cggctgacgc ggagcgtccg ttgggcgaca
     2521 aacaccagga cggggcacag gtacactatc ttgtcacccg gaggcgcgag ggactgcagg
     2581 agcttcaggg agtggcgcag ctgcttcatc cccgtggccc gttgctcgcg tttgctggcg
     2641 gtgtccccgg aagaaatata tttgcatgtc tttagttcta tgatgacaca aaccccgccc
     2701 agcgtcttgt cattggcgaa ttcgaacacg cagatgcagt cggggcggcg cggtcccagg
     2761 tccacttcgc atattaaggt gacgcgtgtg gcctcgaaca ccgagcgacc ctgcagcgac
     2821 ccgcttaaaa gcttggcatt ccggtactgt tggtaaagcc accatggccg atgctaagaa
     2881 cattaagaag ggccctgctc ccttctaccc tctggaggat ggcaccgctg gcgagcagct
     2941 gcacaaggcc atgaagaggt atgccctggt gcctggcacc attgccttca ccgatgccca
     3001 cattgaggtg gacatcacct atgccgagta cttcgagatg tctgtgcgcc tggccgaggc
     3061 catgaagagg tacggcctga acaccaacca ccgcatcgtg gtgtgctctg agaactctct
     3121 gcagttcttc atgccagtgc tgggcgccct gttcatcgga gtggccgtgg cccctgctaa
     3181 cgacatttac aacgagcgcg agctgctgaa cagcatgggc atttctcagc ctaccgtggt
     3241 gttcgtgtct aagaagggcc tgcagaagat cctgaacgtg cagaagaagc tgcctatcat
     3301 ccagaagatc atcatcatgg actctaagac cgactaccag ggcttccaga gcatgtacac
     3361 attcgtgaca tctcatctgc ctcctggctt caacgagtac gacttcgtgc cagagtcttt
     3421 cgacagggac aaaaccattg ccctgatcat gaacagctct gggtctaccg gcctgcctaa
     3481 gggcgtggcc ctgcctcatc gcaccgcctg tgtgcgcttc tctcacgccc gcgaccctat
     3541 tttcggcaac cagatcatcc ccgacaccgc tattctgagc gtggtgccat tccaccacgg
     3601 cttcggcatg ttcaccaccc tgggctacct gatttgcggc tttcgggtgg tgctgatgta
     3661 ccgcttcgag gaggagctgt tcctgcgcag cctgcaagac tacaaaattc agtctgccct
     3721 gctggtgcca accctgttca gcttcttcgc taagagcacc ctgatcgaca agtacgacct
     3781 gtctaacctg cacgagattg cctctggcgg cgccccactg tctaaggagg tgggcgaagc
     3841 cgtggccaag cgctttcatc tgccaggcat ccgccagggc tacggcctga ccgagacaac
     3901 cagcgccatt ctgattaccc cagagggcga cgacaagcct ggcgccgtgg gcaaggtggt
     3961 gccattcttc gaggccaagg tggtggacct ggacaccggc aagaccctgg gagtgaacca
     4021 gcgcggcgag ctgtgtgtgc gcggccctat gattatgtcc ggctacgtga ataaccctga
     4081 ggccacaaac gccctgatcg acaaggacgg ctggctgcac tctggcgaca ttgcctactg
     4141 ggacgaggac gagcacttct tcatcgtgga ccgcctgaag tctctgatca agtacaaggg
     4201 ctaccaggtg gccccagccg agctggagtc tatcctgctg cagcacccta acattttcga
     4261 cgccggagtg gccggcctgc ccgacgacga tgccggcgag ctgcctgccg ccgtcgtcgt
     4321 gctggaacac ggcaagacca tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt
     4381 gacaaccgcc aagaagctgc gcggcggagt ggtgttcgtg gacgaggtgc ccaagggcct
     4441 gaccggcaag ctggacgccc gcaagatccg cgagatcctg atcaaggcta agaaaggcgg
     4501 caagatcgcc gtgtaataat tctagagtcg gggcggccgg ccgcttcgag cagacatgat
     4561 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     4621 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     4681 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     4741 ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatccag gtggcacttt
     4801 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4861 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     4921 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     4981 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5041 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5101 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     5161 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     5221 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     5281 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     5341 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     5401 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     5461 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5521 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5581 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5641 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5701 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     5761 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     5821 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5881 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5941 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6001 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6061 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     6121 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6181 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6241 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6301 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6361 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6421 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6481 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6541 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatggc
     6601 tcgac
//