| 质粒名称: | pAAV-AscI-CAG-LSSmOrange-P2A-EGFP |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000718 |
| 产品用途: | AAV, experess LSSmOrange and EGFP in cytoplasm of mammalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | LSSmOrange, EGFP |
| 物种来源: | Synthetic |
| 密码子优化: | 未优化 |
买家导航
pAAV-AscI-CAG-LSSmOrange-P2A-EGFP载体质粒基本信息
pAAV-AscI-CAG-LSSmOrange-P2A-EGFP质粒图谱载体图谱和pAAV-AscI-CAG-LSSmOrange-P2A-EGFP载体序列质粒序列多克隆位点信息
pAAV-AscI-CAG-LSSmOrange-P2A-EGFP质粒载体简介
pAAV-AscI-CAG-LSSmOrange-P2A-EGFP质粒序列载体序列
LOCUS pAAV-AscI-CAG-LSSmOrange-P2A-EGFP 6292 bp DNA circular SYN 18-JUL-2024
DEFINITION S151D, T166L, W168Y, muated from mChilada.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6292)
AUTHORS Vax
TITLE Direct Submission
JOURNAL Exported May 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT ORIGDB|GenBank
FEATURES Location/Qualifiers
source 1..6292
/lab_host="Mammalian Cells"
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(182..770)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(941..1801)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(1802..1906)
/gene="bla"
/label=AmpR promoter
rep_origin complement(1932..2387)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
misc_feature 2661..2691
/label=AscI-OxE insert
/label=AscI-OxE\insert
/note="/vntifkey=21"
misc_feature 2692..2935
/label=CMV enhancer
/label=CMV\enhancer
/note="/vntifkey=21"
promoter 2942..3219
/label=chicken beta-actin promoter
primer_bind 3623..3661
/label=KpnI-LSSmChilada-F
regulatory 3643..3652
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 3649..4359
/codon_start=1
/product="monomeric orange fluorescent protein with a large
Stokes shift (Shcherbakova et al., 2012)"
/label=LSSmOrange
/note="mammalian codon-optimized"
/translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
FQTVKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGMEASSERMYPEDGALK
GEDKLRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind 4062..4081
/label=mCherry-F
/note="mCherry, forward primer"
primer_bind complement(4336..4381)
/label=AgeI-LSSmChilada-R
CDS 4384..4440
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
misc_feature 4441..4449
/label=Linker
/note="/vntifkey=21"
CDS 4453..5169
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(5146..5166)
/label=AgeI-LSSmChilada-R
misc_feature 5194..5782
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(5665..5676)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 5825..5946
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
ORIGIN
1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
2641 gaagatcgta ggttaattaa ggcgcgcccc taggggagat ggccagccag cccattgacg
2701 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
2761 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
2821 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
2881 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
2941 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
3001 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
3061 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
3121 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
3181 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct
3241 tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
3301 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt
3361 ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga
3421 gggccctttg tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg
3481 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg
3541 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg
3601 tgctgtctca tcattttggc aaagaattgg ggatccggta ccgccaccat ggtgagcaag
3661 ggcgaggaga ataacatggc catcatcaag gagttcatgc gcttcaaggt gcgcatggag
3721 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
3781 ggctttcaga ccgttaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
3841 atcttgtccc ctcagttcac ctacggctcc aaggcctacg tgaagcaccc cgccgacatc
3901 cccgactacc tcaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
3961 gaggacggcg gcgtggtgac cgtgactcag gactcctccc tgcaggacgg cgagttcatc
4021 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
4081 accatgggca tggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
4141 gaggacaagc tcaggctgaa gctgaaggac ggcggccact acacctccga ggtcaagacc
4201 acctacaagg ccaagaagcc cgtgcagttg cccggcgcct acatcgtcga catcaagttg
4261 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
4321 cgccactcca ccggcggcat ggacgagctg tacaagtaac gaccggttca cgtgggaagt
4381 ggggctacaa acttctcact gctgaaacag gccggggatg tggaggaaaa cccaggacca
4441 cacgtgacta gtgtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
4501 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
4561 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
4621 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
4681 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
4741 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
4801 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
4861 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
4921 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
4981 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
5041 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
5101 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
5161 tacaagtaag aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
5221 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
5281 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
5341 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
5401 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
5461 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
5521 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
5581 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
5641 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
5701 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
5761 tccctttggg ccgcctcccc gcatcgatac cgtcgacccg ggcggccgct tcgagcagac
5821 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
5881 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
5941 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag
6001 gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tcttcctaga
6061 gcatggctac gtagataagt agcatggcgg gttaatcatt aactacaagg aacccctagt
6121 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
6181 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg
6241 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ct
//
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