| 质粒名称: | lenti-UCOE-SuperFI-Cas9-BSD |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002156 |
| 产品用途: | Mammalian expression of Cas9 and sgRNA scaffold |
| 存储实验室: | Westlake Genetech |
|
载体骨架基本信息 |
|
| 载体名称: | LV-SFCas9 |
| 表达系统: | 慢病毒，哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | superFiCas9 |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Spacer Extension Restores Cleavage Activity by Enhancing PAM-Distal Interactions in High-Fidelity Cas9
Rong Zheng、Zhike Lu、Rongwei Wei et al.
Nature Structural & Molecular Biology
|
买家导航
lenti-UCOE-SuperFI-Cas9-BSD载体质粒基本信息
lenti-UCOE-SuperFI-Cas9-BSD质粒图谱载体图谱和lenti-UCOE-SuperFI-Cas9-BSD载体序列质粒序列多克隆位点信息
lenti-UCOE-SuperFI-Cas9-BSD质粒载体简介
lenti-UCOE-SuperFI-Cas9-BSD质粒序列载体序列
LOCUS lenti-UCOE-SuperFI-Cas9-BSD 14266 bp DNA circular SYN 22-OCT-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 14266)
AUTHORS White Lab
TITLE Direct Submission
JOURNAL Exported Apr 24, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..14266
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
misc_feature 136..142
/label=cAMP/PKA
misc_feature complement(137..143)
/label=cAMP/PKA
misc_feature 177..186
/label=NFkBBinding
misc_feature 189..195
/label=cAMP/PKA
misc_feature complement(190..196)
/label=cAMP/PKA
misc_feature 272..278
/label=cAMP/PKA
misc_feature complement(273..279)
/label=cAMP/PKA
misc_feature 328..337
/label=NFkBBinding
promoter 381..584
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 382..580
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 425..432
/label=AP1Binding
misc_feature 425..431
/label=AP1
/note="AP1BindingSite"
misc_feature 458..464
/label=cAMP/PKA
misc_feature complement(459..465)
/label=cAMP/PKA
misc_feature 496..505
/label=NFkBBinding
misc_feature complement(506..514)
/label=P5-YY1-60
primer_bind 535..555
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
misc_feature 570..576
/label=CMV-TATA
LTR 598..778
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 598..778
/label=5LTR-zhike
misc_feature 599..619
/label=5LTRRstart(+1mRNA)-Lenti
misc_feature 600..695
/label=HXB2-R
misc_feature 620..657
/label=TARbulge-loop-Lenti
misc_feature 671..676
/label=PolyAMotif
misc_feature 696..778
/label=HXB2-U5
misc_feature 696..778
/label=U5-Lenti
misc_feature complement(780..797)
/label=PBS(tRNALys3Human)
misc_feature 799..817
/label=InterferonResponseElement
misc_feature 825..950
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 888..911
/label=Intron5SplicingSite1
CDS 934..977
/codon_start=1
/label=HXB2-GagStart
/translation="MGARASVLSGGELD"
misc_feature 1025..1030
/label=PolyAMotif2
misc_feature complement(1167..1173)
/label=CMV-TATA
misc_feature 1168..1174
/label=CMV-TATA
misc_feature complement(1345..1351)
/label=CMV-TATA
misc_feature 1346..1352
/label=CMV-TATA
misc_feature 1443..1676
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 1677..2079
/label=RRE-con
misc_feature 1744..1749
/label=PolyAMotif
CDS 1861..1905
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 1939..1945
/label=CMV-TATA
misc_feature 1979..1986
/label=Stem-tetraV1
misc_feature complement(2031..2052)
/label=Intron3SplicingSite2
misc_feature 2046..2053
/label=Stem-tetraV2
misc_feature 2177..2202
/label=cPPT-con
misc_feature 2203..2320
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
misc_feature 2207..2221
/label=PPT(HIV1)
misc_feature 2321..2356
/label=cPPT-con2
misc_feature complement(2325..2330)
/label=PolyAMotif
primer_bind 2342..2382
/label=Gi-enAs-UCOE-F
primer_bind 2344..2375
/label=Gi-Ef1a-As-F
misc_feature 2370..3043
/label=A2UCOE
primer_bind complement(3028..3066)
/label=Gi-enAs-UCOE-R
promoter 3125..4303
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
primer_bind complement(3198..3220)
/label=pCDH1-R
primer_bind complement(3243..3262)
/label=EF1a-R
misc_feature 3293..3299
/label=CMV-TATA
misc_feature 3323..3355
/label=TSS-EF1a
intron 3356..4294
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
misc_feature complement(3462..3467)
/label=OriginTSS
misc_feature complement(3955..3963)
/label=P5-YY1-1
misc_feature complement(3990..3997)
/label=AP1Binding
misc_feature complement(3991..3997)
/label=AP1
/note="AP1BindingSite"
primer_bind 4251..4271
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
primer_bind 4303..4352
/label=In-1-F
promoter 4310..4328
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 4342..4350
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 4351..4404
/codon_start=1
/label=NLS(BE4Max1)
/translation="KRTADGSEFESPKKKRKV"
CDS 4405..8505
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system
Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, K1031D"
/label=SuperFiCas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVDGDDKVDDDDKMIAKSEDEIGDATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
primer_bind 4405..4425
/label=GS-F
primer_bind 4460..4479
/label=CRISPR4
primer_bind complement(4651..4670)
/label=CRISPR7
primer_bind 5251..5270
/label=CRISPR5
primer_bind 5956..5975
/label=CRISPR8
primer_bind complement(6387..6397)
/label=pC1300-F
primer_bind complement(6721..6740)
/label=CRISPR6
CDS 7402..7521
/codon_start=1
/label=Oligo
/translation="YPKLESEFVDGDDKVDDDDKMIAKSEDEIGDATAKYFFYS"
mat_peptide 7429..7431
/label=Y1010D
mat_peptide 7438..7440
/label=Y1013D
mat_peptide 7448..7449
/label=Y1016D
mat_peptide 7453..7455
/label=V1018D
misc_feature 7456..7458
/label=R1019D
misc_feature 7480..7482
/label=Q1027D
misc_feature 7492..7494
/label=K1031D
primer_bind complement(8204..8223)
/label=CRISPR2
CDS 8515..8565
/codon_start=1
/label=NLS(BE4Max2)
/translation="KRTADGSEFEPKKKRKV"
CDS 8584..8631
/codon_start=1
/product="two tandem FLAG(R) epitope tags"
/label=2xFLAG
/translation="DYKDDDDKDYKDDDDK"
primer_bind complement(8620..8641)
/label=GS-R
primer_bind complement(8620..8641)
/label=Amplify-CasF2-R
CDS 8641..8697
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 8698..9096
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
primer_bind complement(9083..9102)
/label=LJB-in-2-R
misc_feature 9121..9709
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(9174..9194)
/label=WPRE-R
/note="WPRE, reverse primer"
misc_feature complement(9207..9212)
/label=PolyAMotif2
CDS complement(9592..9603)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
misc_feature 9715..9780
/label=3LTRcon
misc_feature 9764..9788
/label=PolypurineTract-Lenti
misc_feature 9764..9778
/label=PPT(HIV1)
LTR 9781..10014
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
misc_feature 9814..9848
/label=UpstreamPolyadenylationEnhancer
misc_feature 9834..10014
/label=5LTR-zhike
misc_feature 9835..9855
/label=5LTRRstart(+1mRNA)-Lenti
misc_feature 9836..9931
/label=HXB2-R
misc_feature 9856..9893
/label=TARbulge-loop-Lenti
misc_feature 9907..9912
/label=PolyAMotif
misc_feature 9932..10014
/label=HXB2-U5
misc_feature 9932..10014
/label=U5-Lenti
primer_bind complement(10040..10057)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 10046..10270
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
misc_feature 10136..10141
/label=PolyAMotif
rep_origin 10316..10744
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(10403..10422)
/label=F1ori-R
/note="F1 origin, reverse primer"
misc_feature complement(10606..10611)
/label=PolyAMotif2
primer_bind 10613..10634
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(10753..10773)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 10758..11087
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
enhancer 10759..10842
/label=sv40Enchancer
misc_feature complement(10776..10785)
/label=NFkBBinding
misc_feature complement(10848..10857)
/label=NFkBBinding
rep_origin 10938..11073
/label=SV40 ori
/note="SV40 origin of replication"
misc_feature 10993..11000
/label=Termini
misc_feature complement(10998..11003)
/label=PolyAMotif
primer_bind 11000..11019
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 11135..11182
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 11201..11575
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 11705..11826
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature complement(11711..11716)
/label=PolyAMotif
misc_feature complement(11727..11770)
/label=sv40-USE
misc_feature 11736..11741
/label=PolyAMotif
primer_bind complement(11742..11761)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
misc_feature 11765..11770
/label=PolyAMotif
primer_bind 11796..11815
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(11875..11891)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(11875..11891)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(11888..11910)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 11899..11915
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(11923..11953)
/label=lac promoter
/note="promoter for the E. coli lac operon"
primer_bind complement(11931..11950)
/label=pC1300-F
protein_bind 11968..11989
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(12106..12123)
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 12218..12934
/label=OriPromotor
rep_origin complement(12277..12865)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 12284..12296
/label=Origin-GC
misc_feature 12339..12355
/label=OriginTSS
misc_feature 12354..12361
/label=Origin-TATA
primer_bind complement(12357..12376)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
misc_feature 12381..12397
/label=OriginTSS
misc_feature complement(12403..12423)
/label=5SplicingSite
misc_feature complement(12410..12430)
/label=Intron-5SplicingSite
misc_feature complement(12568..12586)
/label=OriginTSS
misc_feature 12978..12983
/label=PolyAMotif2
CDS complement(13036..13896)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
misc_feature 13078..13097
/label=Intron-3site
misc_feature 13210..13215
/label=PolyAMotif
CDS complement(13334..13712)
/label=ISS
/note="immunostimulatory sequence from the AmpR gene;
contains unmethylated CpG dinucleotides in the context of
5'-AACGTT-3' (Sato et al., 1996)"
primer_bind 13659..13678
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(13897..14001)
/gene="bla"
/label=AmpR promoter
misc_feature 13979..13984
/label=PolyAMotif
misc_feature 14026..14032
/label=cAMP/PKA
primer_bind complement(14076..14095)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
2341 cagagatcca gtttggttaa ttagctagcc gcgtgtggca tctgaagcac caccagcgag
2401 cgagagctag agagaaggaa agccaccgac ttcaccgcct ccgagctgct ccgggtcgcg
2461 ggtctgcagc gtctccggcc ctccgcgcct acagctcaag ccacatccga agggggaggg
2521 agccgggagc tgcgcgcggg gccgccgggg ggaggggtgg caccgcccac gccgggcggc
2581 cacgaagggc ggggcagcgg gcgcgcgccc ggcgggggga ggggccgcgc gccgcgcccg
2641 ctgggaattg gggccctagg gggagggcgg aggcgccgac gaccgcggca cttaccgttc
2701 gcggcgtggc gcccggtggt ccccaagggg agggaagggg gaggcggggc gaggacagtg
2761 accggagtct cctcagcggt ggcttttctg cttggcagcc tcagcggctg gcgccaaaac
2821 cggactccgc ccacttcctc gcccctgcgg tgcgagggtg tggaatcctc cagacgctgg
2881 gggaggggga gttgggagct taaaaactag tacccctttg ggaccacttt cagcagcgaa
2941 ctctcctgta caccaggggt cagttccaca gacgcgggcc aggggtgggt cattgcggcg
3001 tgaacaataa tttgactaga agttgattcg ggtgtttccg gaactgcaaa gatggataaa
3061 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
3121 aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
3181 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
3241 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
3301 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
3361 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
3421 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
3481 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
3541 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
3601 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
3661 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
3721 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
3781 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
3841 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
3901 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
3961 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
4021 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
4081 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
4141 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
4201 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
4261 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgacgtacgt aatacgactc
4321 actataggga gagccagagc cgccaccatg aaacggacag ccgacggaag cgagttcgag
4381 tcaccaaaga agaagcggaa agtcgacaag aagtacagca tcggcctgga catcggcacc
4441 aactctgtgg gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag
4501 gtgctgggca acaccgaccg gcacagcatc aagaagaacc tgatcggagc cctgctgttc
4561 gacagcggcg aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc
4621 agacggaaga accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg
4681 gacgacagct tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac
4741 gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc
4801 accatctacc acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg
4861 atctatctgg ccctggccca catgatcaag ttccggggcc acttcctgat cgagggcgac
4921 ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac
4981 cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgtct
5041 gccagactga gcaagagcag acggctggaa aatctgatcg cccagctgcc cggcgagaag
5101 aagaatggcc tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc caacttcaag
5161 agcaacttcg acctggccga ggatgccaaa ctgcagctga gcaaggacac ctacgacgac
5221 gacctggaca acctgctggc ccagatcggc gaccagtacg ccgacctgtt tctggccgcc
5281 aagaacctgt ccgacgccat cctgctgagc gacatcctga gagtgaacac cgagatcacc
5341 aaggcccccc tgagcgcctc tatgatcaag agatacgacg agcaccacca ggacctgacc
5401 ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt acaaagagat tttcttcgac
5461 cagagcaaga acggctacgc cggctacatt gacggcggag ccagccagga agagttctac
5521 aagttcatca agcccatcct ggaaaagatg gacggcaccg aggaactgct cgtgaagctg
5581 aacagagagg acctgctgcg gaagcagcgg accttcgaca acggcagcat cccccaccag
5641 atccacctgg gagagctgca cgccattctg cggcggcagg aagattttta cccattcctg
5701 aaggacaacc gggaaaagat cgagaagatc ctgaccttcc gcatccccta ctacgtgggc
5761 cctctggcca ggggaaacag cagattcgcc tggatgacca gaaagagcga ggaaaccatc
5821 accccctgga acttcgagga agtggtggac aagggcgctt ccgcccagag cttcatcgag
5881 cggatgacca acttcgataa gaacctgccc aacgagaagg tgctgcccaa gcacagcctg
5941 ctgtacgagt acttcaccgt gtataacgag ctgaccaaag tgaaatacgt gaccgaggga
6001 atgagaaagc ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc
6061 aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag
6121 tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca
6181 taccacgatc tgctgaaaat tatcaaggac aaggacttcc tggacaatga ggaaaacgag
6241 gacattctgg aagatatcgt gctgaccctg acactgtttg aggacagaga gatgatcgag
6301 gaacggctga aaacctatgc ccacctgttc gacgacaaag tgatgaagca gctgaagcgg
6361 cggagataca ccggctgggg caggctgagc cggaagctga tcaacggcat ccgggacaag
6421 cagtccggca agacaatcct ggatttcctg aagtccgacg gcttcgccaa cagaaacttc
6481 atgcagctga tccacgacga cagcctgacc tttaaagagg acatccagaa agcccaggtg
6541 tccggccagg gcgatagcct gcacgagcac attgccaatc tggccggcag ccccgccatt
6601 aagaagggca tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg
6661 cacaagcccg agaacatcgt gatcgaaatg gccagagaga accagaccac ccagaaggga
6721 cagaagaaca gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc
6781 cagatcctga aagaacaccc cgtggaaaac acccagctgc agaacgagaa gctgtacctg
6841 tactacctgc agaatgggcg ggatatgtac gtggaccagg aactggacat caaccggctg
6901 tccgactacg atgtggacca tatcgtgcct cagagctttc tgaaggacga ctccatcgac
6961 aacaaggtgc tgaccagaag cgacaagaac cggggcaaga gcgacaacgt gccctccgaa
7021 gaggtcgtga agaagatgaa gaactactgg cggcagctgc tgaacgccaa gctgattacc
7081 cagagaaagt tcgacaatct gaccaaggcc gagagaggcg gcctgagcga actggataag
7141 gccggcttca tcaagagaca gctggtggaa acccggcaga tcacaaagca cgtggcacag
7201 atcctggact cccggatgaa cactaagtac gacgagaatg acaagctgat ccgggaagtg
7261 aaagtgatca ccctgaagtc caagctggtg tccgatttcc ggaaggattt ccagttttac
7321 aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg
7381 ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgga cggcgacgac
7441 aaggtggacg acgacgataa gatgatcgcc aagagcgagg acgaaatcgg cgacgctacc
7501 gccaagtact tcttctacag caacatcatg aactttttca agaccgagat taccctggcc
7561 aacggcgaga tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg
7621 tgggataagg gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat
7681 atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca aagagtctat cctgcccaag
7741 aggaacagcg ataagctgat cgccagaaag aaggactggg accctaagaa gtacggcggc
7801 ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag
7861 tccaagaaac tgaagagtgt gaaagagcta ctggggatca ccatcatgga aagaagcagc
7921 ttcgagaaga atcccatcga ctttctggaa gccaagggct acaaagaagt gaaaaaggac
7981 ctgatcatca agctgcctaa gtactccctg ttcgagctgg aaaacggccg gaagagaatg
8041 ctggcctctg ccggcgaact gcagaaggga aacgaactgg ccctgccctc caaatatgtg
8101 aacttcctgt acctggccag ccactatgag aagctgaagg gctcccccga ggataatgag
8161 cagaaacagc tgtttgtgga acagcacaag cactacctgg acgagatcat cgagcagatc
8221 agcgagttct ccaagagagt gatcctggcc gacgctaatc tggacaaagt gctgtccgcc
8281 tacaacaagc accgggataa gcccatcaga gagcaggccg agaatatcat ccacctgttt
8341 accctgacca atctgggagc ccctgccgcc ttcaagtact ttgacaccac catcgaccgg
8401 aagaggtaca ccagcaccaa agaggtgctg gacgccaccc tgatccacca gagcatcacc
8461 ggcctgtacg agacacggat cgacctgtct cagctgggag gcgacggaag cggaaaaaga
8521 accgccgacg gcagcgaatt cgagcctaag aaaaagcgga aagtgagcct gggcagcggc
8581 tccgattaca aagatgacga tgacaaagac tacaaggatg atgatgataa gggatccggc
8641 gcaacaaact tctctctgct gaaacaagcc ggagatgtcg aagagaatcc tggaccgatg
8701 gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac
8761 agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc
8821 atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg
8881 ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat
8941 gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat
9001 cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt
9061 gaattgctgc cctctggtta tgtgtgggag ggctaagaat tcgatatcaa gcttatcggt
9121 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
9181 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
9241 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
9301 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
9361 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
9421 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
9481 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
9541 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
9601 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
9661 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
9721 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
9781 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
9841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
9901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
9961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
10021 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
10081 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
10141 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
10201 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
10261 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
10321 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
10381 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
10441 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
10501 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
10561 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
10621 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
10681 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
10741 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
10801 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
10861 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
10921 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
10981 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
11041 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
11101 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
11161 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
11221 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
11281 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
11341 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
11401 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
11461 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
11521 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
11581 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
11641 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
11701 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
11761 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
11821 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
11881 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
11941 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
12001 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
12061 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
12121 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
12181 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
12241 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
12301 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
12361 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
12421 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
12481 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
12541 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
12601 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
12661 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
12721 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
12781 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
12841 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
12901 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
12961 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
13021 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
13081 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
13141 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
13201 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
13261 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
13321 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
13381 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
13441 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
13501 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
13561 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
13621 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
13681 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
13741 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
13801 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
13861 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
13921 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
13981 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
14041 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
14101 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
14161 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
14221 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgtt
//
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