pDJ71

LOCUS       pDJ71              5062 bp DNA     circular SYN 08-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Aug 5, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    6..57
                     /label=important segment
     misc_feature    6..15
                     /label=SRE-1
                     /note="sterol regulatory element-1"
     misc_feature    49..57
                     /label=E-box
     misc_feature    65..213
                     /label=CMVmin
     promoter        87..125
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     194..214
                     /label=181-WKG-593-F
     misc_feature    257..1777
                     /label=important segment
     CDS             257..1759
                     /codon_start=1
                     /product="secreted alkaline phosphatase from human 
                     placenta"
                     /label=SEAP
                     /note="The natural enzyme has a C-terminal transmembrane 
                     sequence that is replaced by a GPI anchor."
                     /translation="MLLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQP
                     AQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDK
                     HVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVV
                     TTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRK
                     YMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTH
                     LMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESR
                     AYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKA
                     RDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFA
                     RGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     primer_bind     complement(305..323)
                     /label=175-WKG-587-R
     primer_bind     750..769
                     /label=Primer 1
     misc_feature    1012..1017
                     /label=putative TEAD-binding sites
     primer_bind     1135..1153
                     /label=392-1151-F2
     primer_bind     1748..1772
                     /label=183-WKG-599-F
     polyA_signal    2819..2953
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     2843..2862
                     /label=58-SV40poly(A)-Rseq
     rep_origin      complement(3315..3903)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3402..3426)
                     /label=225-WKG-1079-F
     primer_bind     3799..3827
                     /label=197-WKG-623-F3
     CDS             complement(4370..4748)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     4418..4440
                     /label=198-WKG-623-F4
     promoter        complement(4933..5037)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtatcac cccacacatc tgacgtcagg atgtcctgca ggacatctat cacgtgactg
       61 caggtcgagc tcggtacccg ggtcgaggta ggcgtgtacg gtgggaggcc tatataagca
      121 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
      181 atagaagaca ccgggaccga tccagcctcc gcggaattcg agctcgcccg gggatcctct
      241 agagtcagct tcctgcatgc tgctgctgct gctgctgctg ggcctgaggc tacagctctc
      301 cctgggcatc atcccagttg aggaggagaa cccggacttc tggaaccgcg aggcagccga
      361 ggccctgggt gccgccaaga agctgcagcc tgcacagaca gccgccaaga acctcatcat
      421 cttcctgggc gatgggatgg gggtgtctac ggtgacagct gccaggatcc taaaagggca
      481 gaagaaggac aaactggggc ctgagatacc cctggccatg gaccgcttcc catatgtggc
      541 tctgtccaag acatacaatg tagacaaaca tgtgccagac agtggagcca cagccacggc
      601 ctacctgtgc ggggtcaagg gcaacttcca gaccattggc ttgagtgcag ccgcccgctt
      661 taaccagtgc aacacgacac gcggcaacga ggtcatctcc gtgatgaatc gggccaagaa
      721 agcagggaag tcagtgggag tggtaaccac cacacgagtg cagcacgcct cgccagccgg
      781 cacctacgcc cacacggtga accgcaactg gtactcggac gccgacgtgc ctgcctcggc
      841 ccgccaggag gggtgccagg acatcgctac gcagctcatc tccaacatgg acattgacgt
      901 gatcctaggt ggaggccgaa agtacatgtt tcgcatggga accccagacc ctgagtaccc
      961 agatgactac agccaaggtg ggaccaggct ggacgggaag aatctggtgc aggaatggct
     1021 ggcgaagcgc cagggtgccc ggtatgtgtg gaaccgcact gagctcatgc aggcttccct
     1081 ggacccgtct gtgacccatc tcatgggtct ctttgagcct ggagacatga aatacgagat
     1141 ccaccgagac tccacactgg acccctccct gatggagatg acagaggctg ccctgcgcct
     1201 gctgagcagg aacccccgcg gcttcttcct cttcgtggag ggtggtcgca tcgaccatgg
     1261 tcatcatgaa agcagggctt accgggcact gactgagacg atcatgttcg acgacgccat
     1321 tgagagggcg ggccagctca ccagcgagga ggacacgctg agcctcgtca ctgccgacca
     1381 ctcccacgtc ttctccttcg gaggctaccc cctgcgaggg agctccatct tcgggctggc
     1441 ccctggcaag gcccgggaca ggaaggccta cacggtcctc ctatacggaa acggtccagg
     1501 ctatgtgctc aaggacggcg cccggccgga tgttaccgag agcgagagcg ggagccccga
     1561 gtatcggcag cagtcagcag tgcccctgga cgaagagacc cacgcaggcg aggacgtggc
     1621 ggtgttcgcg cgcggcccgc aggcgcacct ggttcacggc gtgcaggagc agaccttcat
     1681 agcgcacgtc atggccttcg ccgcctgcct ggagccctac accgcctgcg acctggcgcc
     1741 ccccgccggc accaccgacg ccgcgcaccc gggttaaccc gtggtccccg cgttgcttcc
     1801 tctgctggcc gggaccctgc tgctgctgga gacggccact gctccctgag tgtcccgtcc
     1861 ctggggctcc tgcttcccca tcccggagtt ctcctgctcc ccacctcctg tcgtcctgcc
     1921 tggcctccag cccgagtcgt catccccgga gtccctatac agaggtcctg ccatggaacc
     1981 ttcccctccc cgtgcgctct ggggactgag cccatgacac caaacctgcc ccttggctgc
     2041 tctcggactc cctaccccaa ccccagggac tgcaggttgt gccctgtggc tgcctgcacc
     2101 ccaggaaagg agggggctca ggccatccag ccaccaccta cagcccagtg gcctcgagct
     2161 gcagaagctt ttaaaacagc tctggggttg tacccacccc agaggcccac gtggcggcta
     2221 gtactccggt attgcggtac ccttgtacgc ctgttttata ctcccttccc gtaacttaga
     2281 cgcacaaaac caagttcaat agaagggggt acaaaccagt accaccacga acaagcactt
     2341 ctgtttcccc ggtgatgtcg tatagactgc ttgcgtggtt gaaagcgacg gatccgttat
     2401 ccgcttatgt acttcgagaa gcccagtacc acctcggaat cttcgatgcg ttgcgctcag
     2461 cactcaaccc cagagtgtag cttaggctga tgagtctgga catccctcac cggtgacggt
     2521 ggtccaggct gcgttggcgg cctacctatg gctaacgcca tgggacgcta gttgtgaaca
     2581 aggtgtgaag agcctattga gctacataag aatcctccgg cccctgaatg cggctaatcc
     2641 caacctcgga gcaggtggtc acaaaccagt gattggcctg tcgtaacgcg caagtccgtg
     2701 gcggaaccga ctactttggg tgtccgtgtt tccttttatt ttattgtggc tgcttatggt
     2761 gacaatcaca gattgttatc ataaagcgaa ttggattgcg gccgcttaag ctagcttcga
     2821 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     2881 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     2941 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     3001 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc
     3061 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     3121 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     3181 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     3241 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgtcgggcc
     3301 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     3361 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     3421 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     3481 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     3541 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     3601 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     3661 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     3721 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     3781 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3841 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3901 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     3961 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     4021 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     4081 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     4141 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     4201 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     4261 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     4321 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtgcgca acgttgttgc
     4381 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     4441 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     4501 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     4561 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     4621 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     4681 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     4741 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     4801 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     4861 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     4921 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     4981 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     5041 atttccccga aaagtgccac ct
//