| 质粒名称: | pHybrid-Electra1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000202 |
| 产品用途: | Dual-expression vector. Expresses Electra1 in bacterial cells and co-expresses Electra1 with mScarlet in mammalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pHybrid |
| 表达系统: | 哺乳动物表达、细菌表达 |
| 启动子: | rhaB, CMV, SV40 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | Electra1, mScarlet |
| 物种来源: | Synthetic, Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Dual-expression system for blue fluorescent protein optimization
Papadaki S., Wang X., Wang Y., Zhang H., Jia S., Liu S., Yang M., Zhang D., Jia J., Köster R.W., Namikawa K., Piatkevich K.D.
Sci. Reports 2022 121 12, 1–16 (2022)
|
买家导航
pHybrid-Electra1载体质粒基本信息
pHybrid-Electra1质粒图谱载体图谱和pHybrid-Electra1载体序列质粒序列多克隆位点信息
pHybrid-Electra1质粒载体简介
pHybrid-Electra1质粒序列载体序列
LOCUS pHybrid-Electra1 5609 bp DNA circular SYN 31-MAY-2023
DEFINITION 72.Electra1TSN20211022-0571-00003_1023-ban2_H09_H09_i7.1.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5609)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5609
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 754..858
/gene="bla"
/label=AmpR promoter
CDS 859..1719
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1890..2478
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
enhancer 2689..3068
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3069..3272
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 3305..3423
/label=rhaB promoter
/note="promoter of the E. coli rhaBAD operon, conferring
tight induction with L-rhamnose and repression with
D-glucose in the presence of RhaR and RhaS (Giacalone et
al., 2006)"
primer_bind 3380..3403
/label=rhaB-F
CDS 3464..3481
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
regulatory 3512..3521
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 3518..4231
/codon_start=1
/label=Electra1
/translation="MVSKGEELIEENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
RIKVIEGGPLPFAFDILATSFLFGSKTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
VVTVTQDTSLEDGGLVYNVKVRGVNFPSNGPVMQKKTEGWEPFTEMMYPANGGLRGYTD
IALKVDGDGHLHANIVTTYRSKKTVGDIKMPGVHAVDYRLERVEESDNETYVVLREVAV
AKYSNLGGGMDELYK"
terminator 4252..4338
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind complement(4286..4311)
/label=rrnB-R
polyA_signal 4362..4483
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 4524..4734
/label=SV40 promoter
/note="SV40 early promoter"
promoter 4546..4742
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 4593..4728
/label=SV40 ori
/note="SV40 origin of replication"
regulatory 4743..4752
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 4749..5444
/codon_start=1
/product="bright monomeric red fluorescent protein evolved
from a synthetic template (Bindels et al., 2016)"
/label=mScarlet-I
/note="matures faster than mScarlet"
/translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
STGGMDELYK"
polyA_signal 5449..5496
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
primer_bind complement(5474..5497)
/label=HSV-TK-poly(A)-R
rep_origin join(5576..5609,1..422)
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
61 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
121 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
181 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
241 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
301 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
361 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
421 ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
481 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
541 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
601 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
661 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
721 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
781 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
841 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
901 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
961 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
1021 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
1081 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
1141 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
1201 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
1261 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
1321 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
1381 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
1441 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
1501 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
1561 gccggtgagc gtggctctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
1621 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
1681 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
1741 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
1801 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
1861 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
1921 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
1981 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
2041 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
2101 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
2161 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
2221 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
2281 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
2341 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
2401 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
2461 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
2521 tggccttttg ctcacatggc tcgacagatc ttcaatattg gccattagcc atattattca
2581 ttggttatat agcataaatc aatattggct attggccatt gcatacgttg tatctatatc
2641 ataatatgta catttatatt ggctcatgtc caatatgacc gccatgttgg cattgattat
2701 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
2761 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
2821 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
2881 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
2941 tgccaagtcc gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
3001 agtacatgac cttacgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
3061 ttaccatggt gatgcggttt tggcagtaca ccaatgggcg tggatagcgg tttgactcac
3121 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
3181 aacgggactt tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc
3241 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcactagaa
3301 gcttcaccac aattcagcaa attgtgaaca tcatcacgtt catctttccc tggttgccaa
3361 tggcccattt tcctgtcagt aacgagaagg tcgcgaattc aggcgctttt tagactggtc
3421 gtaacccgtt tttggctaac aggaggaatt aaccatgggg agccaccatc accatcacca
3481 tgctagcctc gagaattcac gcgtggtacc agccaccatg gtgtctaagg gcgaagagct
3541 gatcgaggaa aatatgcgta tgaaggtggt catggaaggt tcggtcaacg gccaccaatt
3601 caaatgcaca ggtgaaggag aaggcagacc gtacgaggga gtgcaaacca tgaggatcaa
3661 agtcatcgag ggaggacccc tgccatttgc ctttgacatt cttgccacgt cgttcctgtt
3721 cggcagcaag acctttatca agtacccggc cgacatccct gatttcttca aacagtcctt
3781 tcctgagggc tttacttggg aaagagttac gagatacgaa gatggtggag tcgtcaccgt
3841 cacgcaggac accagccttg aggatggggg gctcgtctac aacgtcaagg tcagaggggt
3901 aaactttccc tccaatggtc ccgtgatgca gaagaagacc gagggttggg agcctttcac
3961 agagatgatg tatccagcaa atggtggtct gagaggatat accgacatcg cactgaaagt
4021 tgatggtgat ggccatctgc acgccaacat cgtgacaact tacaggtcaa aaaagaccgt
4081 cggggacatc aagatgcccg gtgtccatgc cgttgattac cgcctggaaa gggtcgaaga
4141 gagtgacaat gaaacctacg tagtgctaag agaagtggca gttgccaaat acagcaacct
4201 tggtggtggc atggacgagc tgtacaagta atctagagtc gacccaggca tcaaataaaa
4261 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
4321 ctcctgagta ggacaaatgc ggccgcttcg agcagacatg ataagataca ttgatgagtt
4381 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
4441 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgggt
4501 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
4561 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
4621 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
4681 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
4741 aagccaccat ggtgagcaag ggcgaggcag tgatcaagga gttcatgcgg ttcaaggtgc
4801 acatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
4861 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttct
4921 cctgggacat cctgtcccct cagttcatgt acggctccag ggccttcatc aagcaccccg
4981 ccgacatccc cgactactat aagcagtcct tccccgaggg cttcaagtgg gagcgcgtga
5041 tgaacttcga ggacggcggc gccgtgaccg tgacccagga cacctccctg gaggacggca
5101 ccctgatcta caaggtgaag ctccgcggca ccaacttccc tcctgacggc cccgtaatgc
5161 agaagaagac aatgggctgg gaagcgtcca ccgagcggtt gtaccccgag gacggcgtgc
5221 tgaagggcga cattaagatg gccctgcgcc tgaaggacgg cggccgctac ctggcggact
5281 tcaagaccac ctacaaggcc aagaagcccg tgcagatgcc cggcgcctac aacgtcgacc
5341 gcaagttgga catcacctcc cacaacgagg actacaccgt ggtggaacag tacgaacgct
5401 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtgaccg gcaataaaaa
5461 gacagaataa aacgcacggt gttgggtcgt ttgttcggat ccgggctggc gtaatagcga
5521 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg
5581 ccctgtagcg gcgcattaag cgcggcggg
//
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