| 质粒名称: | pSL1970 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002462 |
| 产品用途: | Mammalian PhCMV-driven ePDZb1-VP64 (TULIP) expression vector (PhCMV-ePDZb1-VP64-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | PhCMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | ePDZb1-VP64 |
| 物种来源: | Synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Rationally designed light-inducible RNA-releasing protein for translational regulation and optogenetic control of gene therapies
NA
NA
|
pSL1970
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL1970载体质粒基本信息
pSL1970质粒图谱载体图谱和pSL1970载体序列质粒序列多克隆位点信息
pSL1970质粒载体简介
pSL1970质粒序列载体序列
LOCUS pSL1970 6124 bp DNA circular SYN 11-FEB-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6124)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Aug 31, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6124
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 820..838
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 855..1436
/label=optimized for human
primer_bind 1437..1462
/label=oSCL2101
primer_bind 1437..1462
/label=oSCL2966
primer_bind complement(1437..1456)
/label=oSCL2835
misc_feature 1437
/label=linker
misc_feature 1441..1442
/label=TetR-CIBN
misc_feature 1441..1442
/label=tetr
primer_bind complement(1443..1492)
/label=oSCL264
primer_bind complement(1443..1473)
/label=oSCL244
CDS 1443..1472
/codon_start=1
/label=linker
/translation="ASGSGGGGDV"
primer_bind 1469..1487
/label=oXQ080
CDS 1473..1622
/label=VP64
misc_feature 1473..1622
/label=VPR
misc_feature 1473..1511
/label=VP16 minimal
primer_bind 1473..1487
/label=oSCL265
misc_feature 1512..1550
/label=VP16 minimal
misc_feature 1551..1589
/label=VP16 minimal
primer_bind complement(1608..1629)
/label=oSCL266
primer_bind complement(1608..1625)
/label=Primer 2
primer_bind complement(1608..1625)
/label=Primer 4
primer_bind complement(1608..1623)
/label=oXQ082
primer_bind complement(1687..1706)
/label=Primer 2
polyA_signal 1724..1948
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 1994..2422
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2436..2765
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2616..2751
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2832..3626
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3800..3921
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3970..3986)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 3994..4010
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4018..4048)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4063..4084
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4372..4957)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5128..5988)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5989..6093)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct aatacgactc actatagggt
841 aaaagcttgc caccatgcct gagctgggct ttagcatcag cggcggcgtt ggaggaagag
901 gcaacccctt cagacccgac gatgacggca tcttcgtgac cagagtgcag ccagagggcc
961 ctgccagcaa gctgctgcag cctggagata agatcatcca ggctaatggc tacagcttca
1021 tcaacatcga gcacggccag gccgtgtccc tgctgaaaac cttccagaac accgtggaac
1081 tgatcatcgt gagagaggtg ggcaacggcg ccaagcagga gatccgggtg cgggtggaaa
1141 aggacggcgg cagcggcgga gtgtccagcg tgcccaccaa cctggaagtg gtggccgcta
1201 cccctacaag cctgctcatc agctgggacg cctaccggga actgcctgtc agctactata
1261 ggatcacata cggcgagaca ggcggcaatt ctccagtgca agagttcacc gtccccggct
1321 ctaaatccac cgccacaatt tctggactga aacctggcgt ggactacacc atcaccgtgt
1381 acgcccacta caactaccac tactactcta gccctatcag catcaactat agaacagcta
1441 gcgccagcgg aagtggcgga ggaggcgacg tcgacgcatt ggacgatttt gatctggata
1501 tgctgggaag tgacgccctc gatgattttg accttgacat gcttggttcg gatgcccttg
1561 atgactttga cctcgacatg ctcggcagtg acgcccttga tgatttcgac ctggacatgc
1621 tgtgagatcc actagtccag tgtggtggaa ttctgcagat atccagcaca gtggcggccg
1681 ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
1741 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
1801 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
1861 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
1921 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
1981 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
2041 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
2101 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
2161 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
2221 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
2281 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
2341 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
2401 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
2461 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
2521 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
2581 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
2641 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
2701 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
2761 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag
2821 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
2881 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
2941 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
3001 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
3061 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
3121 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
3181 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
3241 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
3301 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
3361 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
3421 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
3481 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
3541 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
3601 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
3661 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
3721 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
3781 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
3841 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
3901 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
3961 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
4021 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
4081 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
4141 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
4201 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
4261 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
4321 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
4381 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
4441 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
4501 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
4561 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
4621 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
4681 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
4741 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
4801 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
4861 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg
4921 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
4981 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
5041 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
5101 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
5161 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
5221 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
5281 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
5341 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
5401 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
5461 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
5521 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
5581 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
5641 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
5701 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
5761 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
5821 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
5881 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
5941 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
6001 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
6061 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
6121 cgtc
//
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