pCDH-PCaMKII-Mt-mTagBFP2

LOCUS      pCDH-PCaMKII-Mt-mTagBFP2       7595 bp DNA     circular SYN 02-APR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    2487-pCDH-PCaMKII-Mt-mTagBFP2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 1, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     28..44
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        59..285
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             286..466
                     /label=5' LTR
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    513..638
                     /label=HIV-1 psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1131..1364
                     /label=HIV-1 RRE
                     /note="The Rev response element (RRE) of human 
                     immunodeficiency virus type 1 allows for Rev-dependent mRNA
                     export from the nucleus to the cytoplasm."
     misc_feature    1860..1976
                     /label=HIV-1 cPPT
                     /note="central polypurine tract from pol gene of human 
                     immunodeficiency virus type 1"
     promoter        1989..2359
                     /label=alpha-CamKII Promotor Truncated
     intron          2366..2467
                     /label=Mouse Insulin Intron 1-2
     CDS             2498..2677
                     /codon_start=1
                     /label=Mito-advanced Sequence
                     /translation="MAQRLLLRRFLASVISRKPSQGQWPPLTSRALQTPQCSPGGLTVT
                     PNPARTIYTTRISLT"
     CDS             2693..2752
                     /codon_start=1
                     /product="two tandem HA epitope tags"
                     /label=2xHA
                     /translation="YPYDVPDYAGYPYDVPDYAG"
     CDS             2759..3469
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    3709..4297
                     /label=WPRE
                     /note="woodchuck posttranscriptional regulatory element"
     CDS             complement(4180..4191)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4371..4604
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4676..4797
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      4837..4972
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5005..5021)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5029..5045
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5053..5083)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5098..5119
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5407..5995)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6166..7026)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7027..7131)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctgacgcgtg
       61 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
      121 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
      181 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
      241 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
      301 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
      361 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
      421 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
      481 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
      541 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
      601 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
      661 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
      721 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
      781 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
      841 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
      901 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
      961 ccaccgcaca gcaagcggcc actgatcttc agacctggag gaggagatat gagggacaat
     1021 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     1081 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     1141 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     1201 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     1261 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     1321 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     1381 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     1441 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     1501 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     1561 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     1621 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     1681 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     1741 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     1801 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     1861 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     1921 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     1981 atactagtac ttgtggacta agtttgttca catccccttc tccaaccccc tcagtacatc
     2041 accctgggag aacaaggtcc acttgcttct gggcccacac agtcctgcag tattgtgtat
     2101 ataaggccag ggcaacggag gagcaggttt tgaagtgaaa ggcaggcagg tgttggggag
     2161 gcagttaccg gggcaacggg aacagggcgt ttcggaggtg gttgccatgg ggacctggat
     2221 gctgacgaag gctcgcgagg ctgtgagcag ccacagtgcc ctgctcagaa gccccaagct
     2281 cgtcaatcaa gctggttctc catttgcact caggagcacg ggcaggcgag tggcccctag
     2341 ttctgggagc agagtatcag atatcgtact cttctcagtg ggcctggctc cccagctaag
     2401 acctcaggga cttgaggtag gatatagcct cctctcttac gtgaaacttt tgctatcctc
     2461 aacccaggat atctctagtt gatcagaatt cgccaccatg gctcagcgac ttcttctgag
     2521 gaggttcctg gcctctgtca tctccaggaa gccctctcag ggtcagtggc cacccctcac
     2581 ttccagagcc ctccagaccc cacaatgcag tcctggtggc ctgactgtaa cacccaaccc
     2641 agcccggaca atatacacca cgaggatctc cttgacacct agggccacca tgtacccata
     2701 cgatgttcct gactatgcgg gctatccgta tgacgtcccg gactatgcag gactcgagat
     2761 ggtgtctaag ggcgaagagc tgattaagga gaacatgcac atgaagctat acatggaggg
     2821 caccgtggac aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg
     2881 cacccagacc atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat
     2941 cctggctact agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc
     3001 cgacttcttc aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga
     3061 agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta
     3121 caacgtcaag atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac
     3181 actcggctgg gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag
     3241 aaacgacatg gccctgaagc tcgtgggcgg gagccatctg atcgcaaacg ccaagaccac
     3301 atatagatcc aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta
     3361 cagactggaa agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc
     3421 agtggccaga tactgcgacc tccctagcaa actggggcac aagctcaatc tcgaggcggc
     3481 cgcaggatcc gtttaaacac cggttgtaca agtgagggcc ccatgttcgc cttccgccgc
     3541 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag
     3601 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc
     3661 gccggacccg gctccaccgg atctcgctag agctgaatct aagtcgacaa tcaacctctg
     3721 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     3781 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     3841 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     3901 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     3961 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     4021 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     4081 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     4141 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     4201 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     4261 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac
     4321 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     4381 aattcactcc caacgaagat aagatctgct ttttgcttgt actgggtctc tctggttaga
     4441 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     4501 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     4561 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     4621 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     4681 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     4741 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     4801 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4861 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4921 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt
     4981 tgcagagacc aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
     5041 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     5101 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     5161 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     5221 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5281 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5341 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5401 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5461 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5521 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5581 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5641 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5701 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5761 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     5821 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     5881 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     5941 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     6001 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6061 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6121 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     6181 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6241 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6301 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6361 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6421 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     6481 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     6541 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     6601 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     6661 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     6721 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     6781 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     6841 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     6901 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     6961 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     7021 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     7081 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     7141 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     7201 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     7261 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     7321 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     7381 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     7441 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
     7501 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     7561 gcgattaagt tgggtaacgc cagggttttc cagtc
//