| 质粒名称: | pcDNA5 FRT/TO SF-HERC2 (ShB-R) |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 55613 |
| 存储实验室: | David Chan |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA5 FRT/TO |
| 载体出品公司: | Life Technologies |
| 空载体大小: | 5137 bp |
| 完整质粒大小: | 19695 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | Hygromycin |
| 载体抗性: | Ampicillin, 100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | 3xFLAG tagged HERC2 (WT) |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 14640 bp |
| 基因突变: | HERC2 (a.k.a. D15F37S1, MRT38, SHEP1, jdf2, p528) |
| GenBank编号 | NM_004667.5 |
| 启动子: | CMV |
| 标签/融合蛋白: |
3xFLAG (N terminal on insert)
STREP (N terminal on insert)
|
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | PmeI(未破坏) |
| 3’克隆位点: | NotI (未破坏) |
| 5’测序引物 | CGC AAA TGG GCG GTA GGC GTG |
| 3’测序引物 | TAG AAG GCA CAG TCG AGG C |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA5 FRT/TO SF-HERC2 (ShB-R) was a gift from David Chan (Addgene plasmid # 55613 ; http://n2t.net/addgene:55613 ; RRID:Addgene_55613) |
| 参考文献部分: | Degradation of the Deubiquitinating Enzyme USP33 is Mediated by p97 and the Ubiquitin Ligase HERC2. Chan NC, den Besten W, Sweredoski MJ, Hess S, Deshaies RJ, Chan DC. J Biol Chem. 2014 May 22. pii: jbc.M114.569392. 10.1074/jbc.M114.569392 PubMed 24855649 |
pcDNA5 FRT/TO SF-HERC2 (ShB-R)
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pcDNA5 FRT/TO SF-HERC2 (ShB-R)载体质粒基本信息
pcDNA5 FRT/TO SF-HERC2 (ShB-R)质粒图谱载体图谱和pcDNA5 FRT/TO SF-HERC2 (ShB-R)载体序列质粒序列多克隆位点信息
质粒图谱.png)
pcDNA5 FRT/TO SF-HERC2 (ShB-R)质粒载体简介
pcDNA5 FRT/TO SF-HERC2 (ShB-R)质粒序列载体序列
LOCUS pcDNA5 FRT/TO SF-HERC2 (ShB-R) 19695 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP
tagged wild-type HERC2 in Flp-In TREx cells .
ACCESSION .
VERSION .
KEYWORDS pcDNA5 FRT/TO SF-HERC2 (ShB-R)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 19695)
AUTHORS Chan NC, den Besten W, Sweredoski MJ, Hess S, Deshaies RJ, Chan DC
TITLE Degradation of the Deubiquitinating Enzyme USP33 is Mediated by p97
and the Ubiquitin Ligase HERC2.
JOURNAL J Biol Chem. 2014 May 22. pii: jbc.M114.569392.
PUBMED 24855649
REFERENCE 2 (bases 1 to 19695)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..19695
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 820..838
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 841..859
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 869..893
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 991..1056
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 1066..1089
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
CDS 1096..1116
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQG"
primer_bind complement(15647..15664)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 15653..15877
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(16010..16029)
/label=F1ori-R
/note="F1 origin, reverse primer"
protein_bind 16161..16208
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
CDS 16216..17238
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
"
polyA_signal 17368..17489
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(17405..17424)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 17459..17478
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(17538..17554)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(17538..17554)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(17551..17573)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 17562..17578
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(17586..17616)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 17631..17652
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(17769..17786)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(17940..18528)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(18020..18039)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(18699..19559)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 19322..19341
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(19560..19664)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatac tccagcctcc
961 ggactctagc gctagcacgc gtccaccatg gactacaaag accatgacgg tgattataaa
1021 gatcatgaca tcgattacaa ggatgacgat gacaaggggt cggcctggag ccacccgcag
1081 ttcgaaaaag gatccgagaa cctctacttc caaggctcga gtatgccctc tgaatctttc
1141 tgtttggctg cccaggctcg cctcgactcc aaatggttga aaacagatat acagcttgca
1201 ttcacaagag atgggctctg tggtctgtgg aatgaaatgg ttaaagatgg agaaattgta
1261 tacactggaa cagaatcaac ccagaacgga gagctccctc ctagaaaaga tgatagtgtc
1321 gaaccaagtg gaacaaagaa agaagatctg aatgacaaag agaaaaaaga tgaagaagaa
1381 actcctgcac ctatatatag ggccaagtca attctggaca gctgggtatg gggcaagcaa
1441 ccagatgtga atgaactgaa ggagtgtctt tctgtgctgg ttaaagagca gcaggccctg
1501 gccgtccagt cagccaccac caccctctca gccctgcgac tcaagcagag gctggtgatc
1561 ttggagcgct atttcattgc cttgaataga accgtttttc aggagaatgt caaagttaag
1621 tggaaaagca gcggtatttc tctgcctcct gtggacaaaa aaagttcccg gcctgcgggc
1681 aaaggtgtgg aggggctcgc cagagtggga tcccgagcgg cgctgtcttt tgcctttgcc
1741 ttcctgcgca gggcctggcg atcaggcgag gatgcggacc tctgcagtga gctgttgcag
1801 gagtccctgg acgccctgcg agcacttccc gaggcctcgc tctttgacga gagcaccgtg
1861 tcctctgtgt ggctggaggt ggtggagaga gcgaccaggt tcctcaggtc cgtcgtgacg
1921 ggggatgttc acggaacgcc agccaccaaa gggccaggaa gcatccccct gcaggaccag
1981 cacttggccc tggccatcct gctggagctg gctgtgcaga gaggcacgct gagccaaatg
2041 ttgtctgcca tcctgttgtt gcttcagctg tgggacagcg gggcacagga gactgacaat
2101 gagcgttccg cccagggcac cagcgcccca cttttgccct tgctgcaaag gttccagagc
2161 atcatttgca ggaaggatgc accccactcc gagggcgaca tgcacctttt gtctggccct
2221 ctgagcccca atgagagttt cctgaggtac ctcacccttc cacaagacaa cgagcttgcc
2281 attgatctgc gacaaacggc ggttgttgtc atggcccatt tagaccgtct ggctacgccc
2341 tgtatgcctc cgctgtgtag ctctccgaca tctcataagg gatcattgca agaggtcata
2401 ggttgggggt taataggatg gaaatactat gccaatgtga ttggtccaat ccagtgcgaa
2461 ggcctggcca acctgggagt cacacagatt gcctgtgcag agaagcgttt cctgattctg
2521 tcacgcaatg gccgcgtgta cacacaggcc tataatagtg acacgctggc cccacagctg
2581 gtccaaggcc ttgcctccag aaacattgta aaaattgctg cccattctga tggtcaccac
2641 tacctagcct tggctgctac tggagaggtg tactcctggg gctgtgggga cggcggacgg
2701 ctgggccatg gggacactgt gcctttggag gagcctaagg tgatctccgc cttctctgga
2761 aagcaggccg ggaagcacgt ggtgcacatc gcttgcggga gcacttacag tgcggccatc
2821 actgccgagg gggagctgta cacctggggc cgcgggaact acggccggct gggccatggc
2881 tccagtgagg acgaggccat tccgatgctg gtagccgggc ttaaaggact gaaggtcatc
2941 gatgtggcgt gtgggagtgg ggatgctcaa accctggctg tcactgagaa cgggcaagtg
3001 tggtcttggg gagatggtga ctatgggaaa ttgggcagag gtggtagtga tggctgcaaa
3061 accccaaagc tgattgaaaa gcttcaagac ttggatgtgg tcaaagtccg ctgtggaagt
3121 cagttttcca ttgctttgac gaaagatggc caagtttatt catggggaaa aggtgacaac
3181 cagagacttg gacatggaac agaggaacat gttcgttatc caaaactctt agaaggcttg
3241 caagggaaga aggtgattga tgtggctgca ggctccaccc actgcctggc tctgactgag
3301 gacagcgagg tccacagctg ggggagcaac gaccagtgcc agcactttga caccttgcgc
3361 gtgaccaagc cagaacctgc agcattgcca ggactggaca ccaaacacat agtgggaatt
3421 gcctgtgggc ctgcccagag ctttgcttgg tcatcatgtt ctgagtggtc cattggcctc
3481 cgtgtccctt ttgtggtgga catctgctca atgacttttg agcagctgga tctcctgctt
3541 cggcaggtga gtgaggggat ggatggttcc gcggactggc ccccgcccca ggagaaagag
3601 tgtgtggccg tggcaacgct gaatcttcta cgacttcagt tgcatgctgc cattagtcac
3661 caggttgacc cggaattcct tggtttaggt ctgggcagca tcctcctgaa cagcctgaag
3721 cagacggtgg tgaccctggc cagcagtgcg ggcgtgctga gcaccgtgca gtcggccgcc
3781 caggccgtgc tgcagagtgg ctggtccgtg ctgctgccca ccgcggagga gcgggcccgg
3841 gcactctctg ctctcctgcc ctgcgcagtt tcaggcaatg aagtgaacat aagtccaggt
3901 cgtcgattca tgattgatct tctggtgggc agcttgatgg ctgatggagg gttggagtca
3961 gccttacacg cagccattac tgcagagatc caggatattg aagccaaaaa agaagcacag
4021 aaggaaaaag aaattgatga acaggaagcg aatgcctcaa catttcatag aagcaggact
4081 ccactggata aagaccttat taatacgggg atctgtgagt cttctggcaa acagtgtttg
4141 cctctggttc agctcataca acagcttctt agaaacattg cttctcagac tgtagccaga
4201 ttgaaagatg ttgcccgtcg gatttcatca tgtctggact ttgagcaaca cagtcgtgaa
4261 agatctgctt cattggattt gttactgcgt tttcaacgtt tgcttattag taaactttat
4321 ccaggagaaa gtattggtca gacctcagat atttctagtc cagagctaat gggtgttggt
4381 tccttgctga agaagtacac agccctcctg tgcacgcata tcggggatat actgcctgtg
4441 gccgccagca ttgcttctac cagctggcgg cacttcgcgg aggtggctta cattgtggaa
4501 ggggacttta ctggtgttct ccttccagaa ctagtagttt ctatagtgct tctgctcagt
4561 aaaaatgctg gtctcatgca agaggctgga gctgtacctc tgctgggtgg cctgttggaa
4621 catctggatc ggttcaacca tctggcacca ggaaaggaac gggatgatca tgaagagtta
4681 gcctggcctg gcataatgga gtcatttttt acaggtcaga actgtagaaa taatgaggaa
4741 gtgacactta tacgcaaagc tgatttggag aaccataata aagatggagg cttctggact
4801 gtgattgacg ggaaggtgta tgatataaag gacttccaga cacagtcgtt aacaggaaat
4861 agtattcttg ctcagtttgc aggggaagac ccagtggtag ctttggaagc tgctttgcag
4921 tttgaagaca cccgggaatc catgcacgcg ttttgtgttg gccagtattt ggagcctgac
4981 caagaaatcg tcaccatacc agatctgggg agtctctctt cacctctgat agacacagag
5041 aggaatctgg gcctgcttct cggattacac gcttcgtatt tggcaatgag cacaccgctg
5101 tctcctgtcg agattgaatg tgccaaatgg cttcagtcat ccatcttctc tggaggcctg
5161 cagaccagcc agatccacta cagctacaac gaggagaaag acgaggacca ctgcagctcc
5221 ccagggggca cacctgccag caaatctcga ctctgctccc acagacgggc cctgggggac
5281 cattcccagg catttctgca agccattgca gacaacaaca ttcaggatca caacgtgaag
5341 gactttttgt gtcaaataga aaggtactgt aggcagtgcc atttgaccac accgatcatg
5401 tttccccccg agcatcccgt ggaagaggtc ggtcgcttgt tgttatgttg cctcttaaaa
5461 catgaagatt taggtcatgt ggcattatct ttagttcatg caggtgcact tggtattgag
5521 caagtaaagc acagaacgtt gcctaagtca gtggtggatg tttgtagagt tgtctaccaa
5581 gcaaaatgtt cgctcattaa gactcatcaa gaacagggcc gttcttacaa ggaggtctgc
5641 gctcctgtca tcgaacgttt gagattcctc tttaatgaat tgagacctgc tgtttgtaat
5701 gacctctcta taatgtctaa gtttaaattg ttaagttctt tgccccgttg gaggaggata
5761 gctcaaaaga taattcgaga acgaaggaaa aagagagttc ctaagaagcc agaatctacg
5821 gatgatgaag aaaaaattgg aaacgaagag agtgatttag aagaagcttg cattttgcct
5881 catagtccaa taaatgtgga caagagaccc attgcaatta aatcacccaa ggacaaatgg
5941 cagccgctgt tgagtactgt tacaggtgtt cacaaataca agtggttgaa gcagaatgtg
6001 cagggtcttt atccgcagtc tccactcctc agtacaattg ctgaatttgc ccttaaagaa
6061 gagccagtgg atgtggaaaa aatgagaaag tgcctactaa aacagttgga gagagcagag
6121 gttcgcctgg aagggataga tacaatttta aaactggcga gcaagaattt cttacttcca
6181 tctgtgcagt atgcgatgtt ttgtggatgg caaagactta ttcctgaggg aatcgatata
6241 ggggaacctc ttactgattg tttaaaggat gttgatttga tcccgccttt taatcggatg
6301 ctgctggaag tcacctttgg caagctgtac gcttgggctg tacagaacat tcgaaatgtt
6361 ttgatggatg ccagtgccaa atttaaagag cttggtatcc agccggttcc cctgcaaacc
6421 atcaccaatg agaacccgtc aggaccgagc ctggggacca tcccgcaagc ccgcttcctc
6481 ctggtgatgc tcagcatgct caccctgcag cacggcgcaa acaacctcga ccttctgctc
6541 aattccggca tgctggccct cacgcagacg gcactgcgcc tgattggccc cagttgtgac
6601 aacgttgagg aagatatgaa tgcttctgct caaggtgctt ctgccacagt tttggaagaa
6661 acaaggaagg aaacggctcc tgtgcagctc cctgtttcag gaccagaact ggctgccatg
6721 atgaagattg gaacaagggt catgagaggt gtggactgga aatggggcga tcaggatggg
6781 cctcctccag gcctaggccg cgtgattggt gagctgggag aggacggatg gataagagtc
6841 cagtgggaca caggcagcac caactcctac aggatgggga aagaaggaaa atacgacctc
6901 aagctggcag agctgccggc tgctgcacag ccctcagcag aggattcgga cacagaggat
6961 gactctgaag ccgaacaaac tgaaaggaac attcacccca ctgcaatgat gtttaccagc
7021 actattaact tactgcagac tctttgtctg tctgctggag ttcatgctga gatcatgcag
7081 agcgaagcca ccaagacttt atgcggactg ctgcgaatgt tagtggaaag cggaacgacg
7141 gacaagacat cttctccaaa caggctggtg tacagggagc aacaccggag ctggtgcacg
7201 ctggggtttg tgcggagcat cgctctcacg ccgcaggtat gcggcgccct cagctccccg
7261 cagtggatca cgctgctcat gaaggtcgtg gaagggcacg cacccttcac tgccacctcg
7321 ctgcagaggc agatcttagc tgtgcatttg ttgcaagcag tccttccatc atgggacaag
7381 accgaaaggg cgagggacat gaaatgcctc gtggagaagc tgtttgactt cttgggaagc
7441 ttgctcacta cctgctcctc tgacgtgcca ttactcagag agtccacgct gaggcggcgc
7501 agggtgcgcc cgcaggcctc gctgactgcc acccacagca gcacactggc ggaggaggtg
7561 gtggcactgc tgcgcacgct gcactccctg actcagtgga atgggctcat caacaagtac
7621 atcaactccc agctccgctc catcacccac agctttgtgg gaaggccttc cgaaggggcc
7681 cagttagagg actacttccc cgactccgag aaccctgaag tggggggcct catggcagtc
7741 ctggctgtga ttggaggcat cgatggtcgc ctgcgcctgg gcggtcaagt tatgcacgat
7801 gagtttggag aaggcactgt gactcgcatc accccaaagg gcaaaatcac cgtgcagttc
7861 tctgacatgc ggacgtgtcg cgtttgccca ttgaatcagc tgaaaccact ccctgccgtg
7921 gcctttaatg tgaacaacct gcccttcaca gagcccatgc tgtctgtctg ggctcagttg
7981 gtgaacctcg ctggaagcaa gttagaaaag cacaaaataa agaaatcgac taaacaggcc
8041 tttgcaggac aagtggacct ggacctgctg cggtgccagc agttgaagct atacatcctg
8101 aaagcaggtc gggcgctgct ctcccaccag gataaactgc ggcagatcct gtctcagcca
8161 gctgttcagg agactggaac tgttcacaca gatgatggag cagtggtatc acctgacctt
8221 ggggacatgt ctcctgaagg gccgcagccc cccatgatcc tcttgcagca gctgctggcc
8281 tcggccaccc agccgtctcc tgtgaaggcc atatttgata aacaggaact tgaggctgct
8341 gcactggccg tttgccagtg cttggctgtg gagtccactc acccttcgag cccaggattt
8401 gaagactgca gctccagtga ggccaccacg cctgtcgccg tgcagcacat ccgccctgcc
8461 agagtgaaga ggcgcaagca gtcgcccgtt cccgctctgc cgatcgtggt gcagctcatg
8521 gagatgggat tttccagaag gaacatcgag tttgccctga agtctctcac tggtgcttcc
8581 gggaatgcat ccagcttgcc tggtgtggaa gccttggtcg ggtggctgct ggaccactcc
8641 gacatacagg tcacggagct ctcagatgca gacacggtgt ccgacgagta ttctgacgag
8701 gaggtggtgg aggacgtgga tgatgccgcc tactccatgt ctactggtgc tgttgtgacg
8761 gagagccaga cgtacaaaaa acgagctgat ttcttgagta atgatgatta tgctgtatat
8821 gtgagagaga atattcaggt gggaatgatg gttagatgct gccgagcgta tgaagaagtg
8881 tgcgaaggtg atgttggcaa agtcatcaag ctggacagag atggattgca tgatctcaat
8941 gtgcagtgtg actggcagca gaaagggggc acctactggg ttaggtacat tcatgtggaa
9001 cttataggct atcctccacc aagttcttct tctcacatca agattggtga taaagtgcgg
9061 gtcaaagcct ctgtcaccac accaaaatac aaatggggat ctgtgactca tcagagtgtg
9121 ggggttgtga aggctttcag tgccaatgga aaagatatca ttgtcgactt tccccagcag
9181 tctcactgga ctgggttgct atcagaaatg gagttggtac ccagtattca tcctggggtt
9241 acgtgtgatg gatgtcagat gtttcctatc aatggatcca gattcaaatg cagaaactgt
9301 gatgactttg atttttgtga aacgtgtttc aagaccaaaa aacacaatac caggcataca
9361 tttggcagaa taaatgaacc aggtcagtct gcggtatttt gtggccgttc tggaaaacag
9421 ctgaagcgtt gccacagcag ccagccaggc atgctgctgg acagctggtc ccgcatggtg
9481 aagagcctga atgtgtcgtc ctccgtgaac caggcatccc gtctcattga cggcagcgag
9541 ccctgctggc agtcatcggg gtcgcaagga aagcactgga ttcgtttgga gattttccca
9601 gatgttcttg ttcatagatt aaaaatgatc gtagatcctg ctgacagtag ctacatgccg
9661 tccctggttg tagtgtcagg tggaaattcc ctgaataacc ttattgaact aaagacaatc
9721 aatattaacc cttctgacac cacagtgccc cttctgaatg actgcacaga gtatcacagg
9781 tatattgaaa ttgctataaa gcagtgcagg agctcaggaa tcgattgtaa aatccatggt
9841 ctcatcctgc tgggacggat ccgtgcagaa gaggaagatt tggctgcagt tcctttctta
9901 gcttcggata atgaagagga ggaggatgag aaaggcaaca gcggaagcct cattagaaag
9961 aaggctgctg ggctggaatc agcagctacg ataagaacca aggtgtttgt gtggggcctg
10021 aatgacaagg accagctggg cgggctgaaa ggctccaaga taaaggttcc ttcgttctct
10081 gagacactgt cagctttgaa tgtggtacag gtggctggtg gatctaaaag tttgtttgca
10141 gtgactgtgg aagggaaggt gtatgcctgt ggagaagcca cgaatggccg gctggggctg
10201 ggcatttcca gcgggacggt gcccatccca cggcagatca cagctctcag cagctacgtg
10261 gtcaagaagg tggctgttca ctcaggtggc cggcacgcga cggctttaac tgtcgatgga
10321 aaagtgtttt cgtggggcga aggtgacgat ggaaaacttg gacacttcag cagaatgaac
10381 tgtgacaaac caaggctgat cgaggccctg aaaaccaagc gtatccggga tatcgcctgt
10441 gggagctcgc acagcgcagc cctcacatcc agcggagaac tgtacacctg gggcctcggc
10501 gagtacggcc ggctgggaca tggggataat acgacacagc taaagcccaa aatggtgaaa
10561 gtccttctcg gtcacagagt aatccaggtt gcatgtggga gtagagacgc gcagaccctg
10621 gctctgaccg atgaaggttt ggtattttcc tggggtgatg gtgactttgg aaaactgggc
10681 cggggcggaa gtgaaggctg taacattccc cagaacattg agagactaaa tggacagggg
10741 gtgtgccaga ttgagtgtgg agctcagttc tccctggcgc tcaccaagtc tggagtggtg
10801 tggacatggg gaaaggggga ttacttcaga ttgggccacg gctctgacgt gcacgtgcgg
10861 aaaccacagg tggtggaagg gctgagaggg aagaagatcg tgcatgtggc tgtcggggcc
10921 ctgcactgcc tggcggtcac ggactcgggg caggtgtatg cttggggtga caacgaccac
10981 ggccagcagg gcaatggcac gaccacggtt aacaggaagc ccacactcgt gcaaggctta
11041 gaaggccaga agatcacacg cgtggcttgt gggtcgtccc acagtgtggc gtggacaact
11101 gtggatgtgg ccacgccctc tgtccacgag cccgtcctct tccagactgc aagagaccct
11161 ttaggtgctt cctatttagg cgtgccttca gatgctgatt cttctgctgc cagtaataaa
11221 ataagtggtg caagtaattc taagccaaat cgcccttctc ttgccaagat tctcttgtca
11281 ttggatggaa atctggccaa acagcaggcc ttatcacata ttcttacagc attgcaaatc
11341 atgtatgcca gagatgctgt tgtcggggcc ctgatgccgg ccgccatgat cgccccggtg
11401 gagtgcccct cgttctcctc ggcggcccct tccgacgcat ctgcgatggc tagtcccatg
11461 aatggagaag aatgcatgct ggctgttgat atcgaagaca gactgagtcc aaatccatgg
11521 caagaaaaga gagagattgt ttcctctgag gacgcagtga ccccctctgc agtgactccg
11581 tcggccccct cagcctccgc tcggcctttt atcccagtga cggatgacct gggagccgca
11641 agcatcattg cagaaaccat gaccaaaacc aaagaggatg ttgaaagcca aaataaagca
11701 gcaggtccgg agcctcaggc cttggatgag ttcaccagtc tgctgattgc ggatgacact
11761 cgtgtggtgg tagacctgct caagctgtca gtgtgcagcc gggccgggga caggggcagg
11821 gatgtgctct ccgcggtgct ttccggcatg gggaccgcct acccacaggt ggcagatatg
11881 ctgttggagc tctgtgtcac cgagttggag gatgtggcca cagactcgca gagcggccgc
11941 ctctcttctc agcctgtggt ggtggagagt agccaccctt acaccgacga cacctccacc
12001 agtggcacag tgaagatacc aggtgcagaa ggactcaggg tagaatttga ccggcagtgc
12061 tccacagaga ggcgccacga ccctctcaca gtcatggacg gcgtcaacag gatcgtctcc
12121 gtgcggtcag gccgagagtg gtccgactgg tccagcgagc tgcgcatccc aggggatgag
12181 ttaaagtgga agttcatcag cgatgggtct gtgaatggct ggggctggcg cttcaccgtc
12241 tatcccatca tgccagctgc tggccctaaa gaactcctct ctgaccgctg cgtcctctcc
12301 tgtccatcca tggacttggt gacgtgtctg ttagacttcc gactcaacct tgcctctaac
12361 agaagcatcg tccctcgcct tgcggcctcg ctggcagctt gtgcacagct gagtgcccta
12421 gctgccagtc acagaatgtg ggcccttcag agactgagga agctgcttac aactgaattt
12481 gggcagtcaa ttaacataaa taggctgctt ggagaaaatg atggggaaac aagagctttg
12541 agttttacag gtagtgctct tgctgctttg gtgaaaggtc ttccagaagc tttgcaaagg
12601 cagtttgaat atgaagatcc tattgtgagg ggtggcaaac agctgctcca cagcccattc
12661 tttaaggtac tggtagctct tgcttgtgac ctggagctgg acactctgcc ttgctgtgcc
12721 gagacgcaca agtgggcctg gttccggagg tactgcatgg cctcccgtgt tgctgtggcc
12781 cttgacaaaa gaacaccgtt gccccgtctg tttcttgatg aggtggctaa gaaaattcgt
12841 gaattaatgg cagacagcga aaacatggat gttctgcatg agagccatga catttttaaa
12901 agagagcaag acgaacaact tgtgcagtgg atgaacaggc gaccagatga ctggactctc
12961 tctgctggtg gcagtggaac aatttatgga tggggacata atcacagggg ccagctcggg
13021 ggcattgaag gcgcaaaagt caaagttccc actccctgtg aagcccttgc aactctcaga
13081 cccgtgcagt taatcggagg ggaacagacc ctctttgctg tgacggctga tgggaagctg
13141 tatgccactg ggtatggtgc aggtggcaga ctaggcattg gagggacaga gtcggtgtcc
13201 accccaacat tgcttgaatc cattcagcat gtgtttatta agaaagtagc tgtgaactct
13261 ggaggaaagc actgccttgc cctgtcttca gaaggagaag tttactcttg gggtgaggca
13321 gaagatggga agttggggca tggcaacaga agtccgtgtg accgccctcg tgtcatcgag
13381 tctctgagag gaattgaagt ggtcgatgtt gctgctggcg gagcccacag cgcctgtgtc
13441 acagcagccg gggacctcta cacatggggc aaaggccgct acggccggct ggggcacagc
13501 gacagtgagg accagctgaa gccgaagctg gtggaggcgc tgcagggcca ccgtgtggtt
13561 gacatcgcct gtggcagtgg agatgcccag accctctgcc tcacagatga cgacactgtc
13621 tggtcctggg gggacgggga ctacggcaag ctcggccggg gaggcagcga tggctgtaaa
13681 gtgcctatga agattgattc tcttactggt cttggagtag ttaaagtgga atgcggatcc
13741 cagttttctg ttgcccttac caaatctgga gctgtttata cctggggcaa aggcgattat
13801 cacaggttgg gccatggatc agatgaccat gttcgaaggc ctcggcaggt ccaagggttg
13861 caggggaaga aagtcatcgc catcgccact ggctccctgc actgtgtgtg ctgcacagag
13921 gatggtgagg tttatacatg gggcgacaat gatgagggac aactgggaga cggaaccacc
13981 aatgccatcc agaggcctcg gttggtagct gcccttcagg gtaagaaggt caaccgtgtg
14041 gcctgtggct cagcacatac cctcgcctgg tcgaccagca agcccgccag tgctggcaaa
14101 ctccctgcac aggtccccat ggagtacaat cacctgcagg agatccccat cattgcgctg
14161 aggaaccgtc tgctgctgct gcaccacctc tccgagctct tctgcccctg catccccatg
14221 ttcgacctgg aaggctcgct cgacgaaact ggactcgggc cttctgttgg gttcgacact
14281 ctccgaggaa ttctgatatc ccagggaaag gaggcggctt tccggaaagt agtacaagca
14341 actatggtac gcgatcgtca gcatggcccc gtcgtggagc tgaaccgcat ccaggtcaaa
14401 cgatcaagga gcaaaggcgg gctggccggc cccgacggca ccaagtctgt ctttgggcag
14461 atgtgtgcta agatgagctc gtttggtccc gacagcctcc tccttcctca ccgtgtctgg
14521 aaagtcaagt ttgtgggtga atctgtggat gactgtgggg gcggctacag cgagtccata
14581 gctgagatct gtgaggagct gcagaacgga ctcacgcccc tgctgatcgt gacacccaac
14641 gggagggatg agtctggggc caaccgagac tgctacctgc tcagcccggc cgccagagca
14701 cccgtgcaca gcagcatgtt ccgcttcctg ggtgtgttgc tgggcattgc catccgaacc
14761 gggagtcccc tgagcctcaa ccttgccgag cctgtctgga agcagctggc tgggatgagc
14821 ctcaccatcg cggacctcag tgaggttgat aaggatttta ttcctggact catgtacatc
14881 cgagacaatg aagccacctc agaggagttt gaagccatga gcctgccctt cacagtgcca
14941 agtgccagtg gccaggacat tcagttgagc tccaagcaca cacacatcac cctggacaac
15001 cgcgcggagt acgtgcggct ggcgataaac tatagactcc atgaatttga tgagcaggtg
15061 gctgctgttc gggaaggaat ggcccgcgtt gtgcctgttc ccctcctctc tctgttcacc
15121 ggctacgaac tggagacgat ggtgtgtggc agccctgaca tcccgctgca ccttctcaag
15181 tcggtggcca cctataaagg catcgagcct tccgcatcgc tgatccagtg gttctgggag
15241 gtgatggagt ccttctccaa cacagagcgc tctcttttcc ttcgcttcgt ctggggccgg
15301 acgaggctgc ccaggaccat cgccgacttc cggggccgag acttcgtcat ccaggtgttg
15361 gataaataca accctccaga ccacttcctc cctgagtcct acacctgttt cttcttgctg
15421 aagctgccca ggtattcctg caagcaggtg ctggaggaga agctcaagta cgccatccac
15481 ttctgcaagt ccatagacac agatgactac gctcgcatcg cacttacagg agagccagcc
15541 gccgacgaca gcagcgacga ttcagataac gaggatgtcg actcctttgc ttcggactct
15601 acacaagatt atttaacagg acactaagtt taaacccgct gatcagcctc gactgtgcct
15661 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
15721 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
15781 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
15841 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
15901 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
15961 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
16021 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
16081 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
16141 ggtgatggtt cacgtaccta gaagttccta ttccgaagtt cctattctct agaaagtata
16201 ggaacttcct tggccaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
16261 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
16321 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
16381 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
16441 gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag
16501 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg
16561 gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
16621 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
16681 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag
16741 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
16801 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
16861 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
16921 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt
16981 gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag
17041 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc
17101 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc
17161 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt
17221 ccgagggcaa aggaatagca cgtactacga gatttcgatt ccaccgccgc cttctatgaa
17281 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
17341 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
17401 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
17461 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
17521 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
17581 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
17641 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
17701 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
17761 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
17821 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
17881 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
17941 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
18001 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
18061 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
18121 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
18181 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
18241 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
18301 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
18361 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
18421 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
18481 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
18541 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
18601 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
18661 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
18721 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
18781 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
18841 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
18901 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
18961 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
19021 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
19081 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
19141 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
19201 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
19261 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
19321 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
19381 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
19441 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
19501 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
19561 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
19621 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
19681 gtgccacctg acgtc
//
