| 质粒名称: | pGL3-DNMT1-hCtRNA-Nicking/PegRNA-PGK-puroR |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002191 |
| 产品用途: | The hCtRNA simultaneously drives the expression of both the targeting DNMT1 gene NickingRNA and pegRNA, harboring puroR selection marker. |
| 存储实验室: | Zhuobin Liang Lab 梁卓斌课题组 |
|
载体骨架基本信息 |
|
| 载体名称: | pGL3 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | hCtRNA |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | DNMT1-nicking-sgRNA-pegRNA |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
TRU-PE: A Universal, Trackable Prime Editor Toolkit for Robust Single-and Multi-Locus Genome Engineering
Zhichao Qiu, Keke Sun, Qingwei Zeng, Ziwei Luo, Xinran Liu, Yaping Li, Xiang Lei, Ruilin Zhao, Zhen Zhang, Damia Romero-Moya, Yuan Yang, Xuan Wang, Ehsan Hashemi, Joe Z. Zhang, Xiaolong Wang, Alessandra Giorgetti, Zhuobin Liang
bioRxiv 2026.02.20.706938
|
pGL3-DNMT1-hCtRNA-Nicking/PegRNA-PGK-puroR
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pGL3-DNMT1-hCtRNA-Nicking/PegRNA-PGK-puroR载体质粒基本信息
pGL3-DNMT1-hCtRNA-Nicking/PegRNA-PGK-puroR质粒图谱载体图谱和pGL3-DNMT1-hCtRNA-Nicking/PegRNA-PGK-puroR载体序列质粒序列多克隆位点信息
pGL3-DNMT1-hCtRNA-Nicking/PegRNA-PGK-puroR质粒载体简介
pGL3-DNMT1-hCtRNA-Nicking/PegRNA-PGK-puroR质粒序列载体序列
LOCUS pGL3-DNMT1-hCtRNA-Nicking/PegRNA-PGK-puroR 4942 bp DNA circular SYN 07-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4942)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 20, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4942
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 4..23
/label=OL1915
primer_bind 21..55
/label=OL2640
primer_bind complement(21..44)
/label=OL459
primer_bind 22..61
/label=OL2721
primer_bind 24..40
/label=OL1147
primer_bind 24..40
/label=OL2857
primer_bind 37..61
/label=OL1451
misc_feature 41..115
/label=hCtRNA
primer_bind 56..79
/label=OL464
primer_bind complement(98..115)
/label=OL465
primer_bind complement(101..116)
/label=OL2643
primer_bind complement(102..136)
/label=OL2641
primer_bind 116..164
/label=OL2722
primer_bind 116..152
/label=OL2642
misc_feature 116..136
/label=DNMT1 nickingRNA
misc_RNA 137..212
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind 137..177
/label=OL462
primer_bind complement(195..231)
/label=OL463
misc_feature 213..287
/label=hCtRNA
primer_bind 213..233
/label=OL2721
primer_bind 213..233
/label=OL1451
primer_bind 213..227
/label=OL2640
primer_bind 228..251
/label=OL464
primer_bind complement(270..287)
/label=OL465
primer_bind complement(273..307)
/label=OL2643
primer_bind complement(274..288)
/label=OL2641
primer_bind 288..311
/label=OL2644
misc_feature 288..307
/label=DNMT1 spacer
primer_bind 292..307
/label=OL2645
misc_RNA 308..383
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind 308..348
/label=OL462
primer_bind 308..335
/label=OL2722
primer_bind 308..323
/label=OL2642
primer_bind complement(366..383)
/label=OL463
misc_feature 384..394
/label=RT template
primer_bind complement(388..431)
/label=OL2723
misc_feature 390
/label=C352A
primer_bind complement(392..431)
/label=OL2645
primer_bind 392..407
/label=OL2643
misc_feature 395..407
/label=PBS
primer_bind complement(411..430)
/label=OL2858
primer_bind complement(414..430)
/label=OL1148
primer_bind complement(420..439)
/label=OL1916
misc_feature 451..568
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 617..1127
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 1046..1064
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 1149..1748
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(1149..1168)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 1645..1665
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
polyA_signal 1895..2016
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1906..1925)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 1960..1979
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(2264..2281)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2435..3023)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2515..2534)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3194..4054)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3817..3836
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4055..4159)
/gene="bla"
/label=AmpR promoter
rep_origin 4186..4641
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4273..4292)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4483..4504
/label=F1ori-F
/note="F1 origin, forward primer"
polyA_signal 4772..4820
/note="synthetic polyadenylation signal"
misc_feature 4834..4925
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 4874..4893
/label=RVprimer3
/note="pGL3 vector, forward primer"
ORIGIN
1 agtgaacgga tctcgacggt atcgatcacg agactagcct agagggggta tagctcagtg
61 gtagagcatt tgactgcaga tcaagaggtc cccggttcaa atccgggtgc cccctgccct
121 tcagctaaaa taaagggttt tagagctaga aatagcaagt taaaataagg ctagtccgtt
181 atcaacttga aaaagtggca ccgagtcggt gcagaggggg tatagctcag tggtagagca
241 tttgactgca gatcaagagg tccccggttc aaatccgggt gccccctgat tcctggtgcc
301 agaaacagtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
361 aaaaagtggc accgagtcgg tgcgtcacca ctgtttctgg caccaggttt ttttctcgac
421 ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
481 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
541 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
601 ttggccgcgg ctcgaggggg ttggggttgc gccttttcca aggcagccct gggtttgcgc
661 agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggactc
721 gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc
781 ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt
841 gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc
901 agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag
961 ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag
1021 tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt
1081 cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg gatccaccgg
1141 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
1201 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
1261 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
1321 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
1381 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
1441 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
1501 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
1561 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
1621 ccttcctgga aacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
1681 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
1741 gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg
1801 catcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttctag
1861 agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga gtttggacaa
1921 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
1981 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
2041 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
2101 tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg agagccttca acccagtcag
2161 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat
2221 catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc actgactcgc
2281 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
2341 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
2401 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
2461 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
2521 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
2581 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
2641 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
2701 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
2761 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
2821 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
2881 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
2941 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
3001 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
3061 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
3121 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
3181 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
3241 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
3301 taccatctgg ccccagtgct gcaatgatac cgcgggaccc acgctcaccg gctccagatt
3361 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
3421 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
3481 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
3541 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
3601 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
3661 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
3721 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
3781 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
3841 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
3901 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
3961 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
4021 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
4081 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
4141 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg
4201 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
4261 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
4321 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
4381 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
4441 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
4501 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
4561 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
4621 aaatattaac gcttacaatt tgccattcgc cattcaggct gcgcaactgt tgggaagggc
4681 gatcggtgcg ggcctcttcg ctattacgcc agcccaagct accatgataa gtaagtaata
4741 ttaaggtacg ggaggtactt ggagcggccg caataaaata tctttatttt cattacatct
4801 gtgtgttggt tttttgtgtg aatcgatagt actaacatac gctctccatc aaaacaaaac
4861 gaaacaaaac aaactagcaa aataggctgt ccccagtgca agtgcaggtg ccagaacatt
4921 tctctatcga taggtaccga tt
//
pGL3-DNMT1-hCtRNA-Nicking/PegRNA-PGK-puroR载体图谱质粒图谱pdf版和pGL3-DNMT1-hCtRNA-Nicking/PegRNA-PGK-puroR载体序列质粒序列等相关资料下载
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