| 质粒名称: | dmBACCS2-IRES-dOrai-IRES-GFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 72893 |
| 存储实验室: | Takao Nakata |
|
载体骨架基本信息 |
|
| 载体名称: | pWPRE/C1 (modified pEGFP-C1) |
| 载体出品公司: | Modified from Clontech |
| 空载体大小: | 4581 bp |
| 完整质粒大小: | 9127 bp |
| 载体修饰: | 将土拨鼠肝炎病毒转录后调节元件（WPRE）插入pEGFP-C1的BamHI位点，并去除EGFP。 |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 卡那霉素50 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | dmBACCS2-IRES-dOrai-IRES-GFP |
| 物种来源: | D. melanogaster (fly); Avena sativa (oat) |
| 插入大小: | 4546 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | CMV |
| 标签/融合蛋白: | IRES-GFP（主干上的C终端） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | XhoI(未破坏) |
| 3’克隆位点: | SalI(未破坏) |
| 5’测序引物 | CMV Forward |
| 3’测序引物 | WPRE-R |
|
文献引用方法 |
|
| 材料和方法部分: | dmBACCS2-IRES-dOrai-IRES-GFP was a gift from Takao Nakata (Addgene plasmid # 72893 ; http://n2t.net/addgene:72893 ; RRID:Addgene_72893) |
| 参考文献部分: | Light generation of intracellular Ca(2+) signals by a genetically encoded protein BACCS. Ishii T, Sato K, Kakumoto T, Miura S, Touhara K, Takeuchi S, Nakata T. Nat Commun. 2015 Aug 18;6:8021. doi: 10.1038/ncomms9021. 10.1038/ncomms9021 PubMed 26282514 |
买家导航
dmBACCS2-IRES-dOrai-IRES-GFP载体质粒基本信息
dmBACCS2-IRES-dOrai-IRES-GFP质粒图谱载体图谱和dmBACCS2-IRES-dOrai-IRES-GFP载体序列质粒序列多克隆位点信息
dmBACCS2-IRES-dOrai-IRES-GFP质粒载体简介
dmBACCS2-IRES-dOrai-IRES-GFP质粒序列载体序列
LOCUS dmBACCS2-IRES-dOrai-IRES-GFP 9127 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Mammalian expression of dmBACCS2, a Drosophila melanogaster blue
light-activated Ca2+ channel switch, along with dOrai and GFP.
ACCESSION .
VERSION .
KEYWORDS dmBACCS2-IRES-dOrai-IRES-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9127)
AUTHORS Ishii T, Sato K, Kakumoto T, Miura S, Touhara K, Takeuchi S, Nakata
T
TITLE Light generation of intracellular Ca(2+) signals by a genetically
encoded protein BACCS.
JOURNAL Nat Commun. 2015 Aug 18;6:8021. doi: 10.1038/ncomms9021.
PUBMED 26282514
REFERENCE 2 (bases 1 to 9127)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9127
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 519..539
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 603..612
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
misc_feature 2186..2759
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
misc_feature 2209..2761
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
regulatory 3281..3290
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
misc_feature 3843..4416
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
misc_feature 3866..4418
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 4418..5137
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 4418..5134
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(4463..4484)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4724..4743)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 5071..5092
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 5182..5770
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5235..5255)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5653..5664)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 5915..6036
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5952..5971)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6006..6025
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(6043..6498)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6180..6201)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 6392..6411
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 6525..6629
/gene="bla"
/label=AmpR promoter
promoter 6631..6988
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(6654..6674)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 6839..6974
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6901..6920
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 7023..7817
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(7077..7096)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 7687..7706
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(8005..8024)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 8049..8096
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 8425..9013
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8914..8933
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcctcg
601 aggccaccat gggaccggtc ggaggaagcg gtggatcttt ggctactaca cttgaacgta
661 ttgagaagaa ctttgtcatt actgacccaa gattgccaga taatcccatt atattcgcgt
721 ccgatagttt cttgcagttg acagaatata gccgtgaaga aattttggga agaaactgca
781 ggtttctaca aggtcctgaa actgatcgcg cgacagtgag aaaaattaga gatgccatag
841 ataaccaaac agaggtcact gttcagctga ttaattatac aaagagtggt aaaaagttct
901 ggaacctctt tcacttgcag cctatgcgag atcagaaggg agatgtccag tactttattg
961 gggttcagtt ggatggaact gagcatgtcc gagatgctgc cgagagagag ggagtcatgc
1021 tgattaagaa aactgcagaa aatctgcaat catggcttca atacacatat gaactagaaa
1081 gtaagaatca tcagaagaag cgcacgtcgg ctgagaagca gctacagtcg gccagagagg
1141 cttgtgagaa attgcgtaag aaacggtcaa gtttggtggg tgcgttcgtt tccacgcacg
1201 gaaagagtat tgatgatgtg gatcggtcga ttgttgaggc acggaatgcc ctcggagatg
1261 taacaaacga gctgcaagaa cgactgcatc gctggaagca aatcgagacg tgccttggct
1321 taaacattgt gaacaacaat ggtctgcccg gaggatccgg aggaagtgga ggaggtatcc
1381 tgcagtctcg gggtggcagt ggtggttcag gcggttctgg atctttggct actacacttg
1441 aacgtattga gaagaacttt gtcattactg acccaagatt gccagataat cccattatat
1501 tcgcgtccga tagtttcttg cagttgacag aatatagccg tgaagaaatt ttgggaagaa
1561 actgcaggtt tctacaaggt cctgaaactg atcgcgcgac agtgagaaaa attagagatg
1621 ccatagataa ccaaacagag gtcactgttc agctgattaa ttatacaaag agtggtaaaa
1681 agttctggaa cctctttcac ttgcagccta tgcgagatca gaagggagat gtccagtact
1741 ttattggggt tcagttggat ggaactgagc atgtccgaga tgctgccgag agagagggag
1801 tcatgctgat taagaaaact gcagaaaatc tgcaatcatg gcttcaatac acatatgaac
1861 tagaaagtaa gaatcatcag aagaagcgca cgtcggctga gaagcagcta cagtcggcca
1921 gagaggcttg tgagaaattg cgtaagaaac ggtcaagttt ggtgggtgcg ttcgtttcca
1981 cgcacggaaa gagtattgat gatgtggatc ggtcgattgt tgaggcacgg aatgccctcg
2041 gagatgtaac aaacgagctg caagaacgac tgcatcgctg gaagcaaatc gagacgtgcc
2101 ttggcttaaa cattgtgaac aacaatggtc tgcccggcgg ctctggaggt agcggactag
2161 tttgagtcga gccgcggcaa ttccgcccct ctccctcccc cccccctaac gttactggcc
2221 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
2281 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
2341 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
2401 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
2461 accccccacc tggcaacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
2521 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
2581 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
2641 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
2701 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat
2761 atgtctgtgt ggaccacggc caacaattcg ggcctagaga cgccaacaaa gtcgccgatc
2821 acgtcgtcgg ttccgcgggc atcgagaagt tctgcggtga tcaccactgg aaatcaccag
2881 cagcaccact tccagcacgt tgtggccgcc gccgttgcag ccgccacttc agtggccacc
2941 ggtcatcagt tccagcagca gttcccgctg cacgcacatc cgcatccgca tccacagcac
3001 cacagcaaca gtcccacggg tagcggcagc aacagcaaca acagcgcagg tttccagcgc
3061 accagcatca gcaactcgct gctgcagttc ccgccgcccc cgcccccttc ctcacagaac
3121 caggcgaagc cgcgcggaca ccatcgcacc gccagcagca gcatgtcgca atccggcgag
3181 gatctccact cgcccaccta cctgtcctgg cggaagctcc agcttagtcg ggccaagcta
3241 aaggcatcca gcaagacgtc cgccctgctc tccggattcg ccatggtggc gatggtggag
3301 gtgcaactgg atcacgatac gaatgtacca ccgggcatgc tgatagcctt cgccatctgc
3361 accaccctcc tggtggcggt gcacatgctg gccctgatga tcagcacctg tatcctgcca
3421 aatattgaga cggtgtgtaa tttgcatagt atttccctgg tccacgaatc gccacacgag
3481 cgtctccatt ggtacattga aacggcgtgg gcattctcca cgctgctggg actcatactc
3541 tttctactgg agatagccat cctgtgctgg gtgaaattct atgacctaag tccgccggcg
3601 gcttggtcag cgtgtgtggt cctcataccg gtgatgatca tctttatggc cttcgccatt
3661 cacttctatc gctccctggt gtcgcacaaa tatgaggtga cagtctcggg catccgtgag
3721 ctggagatgc tcaaggagca gatggagcag gatcatttgg agcatcacaa taacatacga
3781 aataatggca tgaactatgg cgcatccggg gacattgtct aggtcgagcc gcggcaattc
3841 cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
3901 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
3961 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
4021 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
4081 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg caacaggtgc
4141 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
4201 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
4261 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
4321 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
4381 ggggacgtgg ttttcctttg aaaaacacga tgataatatg gtgagcaagg gcgaggagct
4441 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
4501 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
4561 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
4621 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
4681 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
4741 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
4801 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
4861 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
4921 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
4981 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
5041 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
5101 cgggatcact ctcggcatgg acgagctgta caagtaaccg cggtcgacgg taccgcgggc
5161 ccgggatcta agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
5221 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
5281 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
5341 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
5401 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
5461 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
5521 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
5581 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
5641 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
5701 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
5761 gcctccccgc atcgataccg tggatcgatc caccggatct agataactga tcataatcag
5821 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
5881 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
5941 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
6001 tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata
6061 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
6121 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
6181 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
6241 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
6301 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
6361 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
6421 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
6481 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
6541 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
6601 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt
6661 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
6721 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
6781 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
6841 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
6901 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
6961 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc
7021 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
7081 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
7141 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
7201 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
7261 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
7321 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
7381 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
7441 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
7501 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
7561 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
7621 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
7681 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
7741 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
7801 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
7861 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
7921 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
7981 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
8041 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
8101 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
8161 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
8221 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
8281 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
8341 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
8401 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
8461 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
8521 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
8581 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
8641 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
8701 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
8761 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
8821 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
8881 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
8941 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
9001 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
9061 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
9121 catgcat
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