pDJ684

LOCUS       pDJ684           6532 bp DNA     circular SYN 10-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Aug 5, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6532
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     78..99
                     /label=oYW269
                     /note="LA-NheI-F"
     misc_feature    196..226
                     /label=SleepingBeauty-ITR_Lo
                     /label=nonstandard type:  ITR
     misc_feature    392..423
                     /label=SleepingBeauty-ITR_Li
                     /label=nonstandard type:  ITR
     primer_bind     451..498
                     /label=oYW336
     primer_bind     460..476
                     /label=oYW181
                     /note="P_hCMV-F"
     primer_bind     complement(460..476)
                     /label=oYW179
                     /note="ITR PCR F"
     misc_feature    477..1580
                     /label=EF-1a
                     /note="/vntifkey=21"
     primer_bind     477..498
                     /label=oYW338
                     /note="LA-NruI-PEF1a PCR-F"
     promoter        493..1671
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     primer_bind     496..510
                     /label=oYW43
                     /note="LA-MluI-hEF1a PCR F"
     primer_bind     496..510
                     /label=oYW183
                     /note="P_hEF1a PCR F"
     primer_bind     604..627
                     /label=oDJ223-1
     primer_bind     605..627
                     /label=oYW355
                     /note="LA-NruI-PEF1a PCR-F"
     primer_bind     605..624
                     /label=oDJ8-1
     primer_bind     complement(611..630)
                     /label=EF1a-R
     primer_bind     complement(671..690)
                     /label=oYW45
                     /note="hEF1a mutant TOP motif LA-R"
     primer_bind     673..713
                     /label=oYW46
                     /note="hEF1a mutant TOP motif RA-F"
     intron          724..1662
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     937..956
                     /label=oYW361
                     /note="PEF1a mid seq primer"
     primer_bind     1076..1093
                     /label=oYW47
                     /note="hEF1a-Seq-F"
     primer_bind     1438..1459
                     /label=oYW285
                     /note="PEF1a-F for pYW136/137"
     primer_bind     complement(1551..1560)
                     /label=oYW346
     primer_bind     1619..1639
                     /label=pEF-F/EF-1aFwd
     primer_bind     complement(1640..1659)
                     /label=oYW184
                     /note="P_hEF1a PCR R"
     primer_bind     complement(1643..1697)
                     /label=oYW337
     primer_bind     complement(1643..1672)
                     /label=oYW348
                     /note="RA-PEF1a PCR R"
     primer_bind     complement(1643..1672)
                     /label=oYW354
                     /note="RA-PEF1a PCR R"
     primer_bind     complement(1643..1671)
                     /label=oYW339
                     /note="RA-NheI-PEF1a PCR R"
     primer_bind     1643..1659
                     /label=oYW180
                     /note="Puro PCR R"
     primer_bind     1670..1707
                     /label=oYW333
                     /note="LA-AgeI-PacI-EcoRV-IRES PCR-F"
     misc_feature    1730..2303
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1776..1795)
                     /label=pIRES2-EGFP.P3
     primer_bind     complement(1825..1844)
                     /label=IRES-R
     primer_bind     2301..2312
                     /label=oDJ199-3
     primer_bind     complement(2316..2361)
                     /label=oYW335
                     /note="RA-SalI-IRES PCR R"
     primer_bind     complement(2316..2350)
                     /label=oYW344
     primer_bind     complement(2316..2350)
                     /label=oYW345
     primer_bind     complement(2316..2335)
                     /label=oYW334
                     /note="IRES PCR R"
     primer_bind     2336..2373
                     /label=oYW193
                     /note="LA-PuroR-F"
     primer_bind     2351..2373
                     /label=oYW180
                     /note="Puro PCR R"
     CDS             2353..2952
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2838..2891)
                     /label=oYS113
     primer_bind     complement(2937..2952)
                     /label=oYW194
                     /note="PuroR-R"
     primer_bind     complement(2995..3012)
                     /label=BGH/pcDNA3.1R
     primer_bind     complement(2995..3012)
                     /label=pcDNA3.1-R(BGH-R)
     primer_bind     2998..3017
                     /label=oYW188
                     /note="bGH poly A"
     polyA_signal    3001..3225
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3005..3022)
                     /label=oYW190
                     /note="BGH poly A R"
     primer_bind     complement(3067..3085)
                     /label=oYW303
                     /note="PolyA tail-bGH pA"
     primer_bind     complement(3067..3085)
                     /label=oYW57
                     /note="bGH seq primer R"
     primer_bind     complement(3070..3097)
                     /label=oYW300
                     /note="PolyA tail-bGH pA"
     primer_bind     complement(3070..3093)
                     /label=oYW302
                     /note="bGH pA R"
     primer_bind     3070..3089
                     /label=W2F0627-P099-D03
                     /note="Sequencing primer"
     primer_bind     3128..3147
                     /label=BGH-polyA-R
     primer_bind     3208..3254
                     /label=oYW203
                     /note="LA-P_CMV-F"
     primer_bind     complement(3208..3231)
                     /label=oYW202
                     /note="BGH polyA-R"
     primer_bind     complement(3208..3225)
                     /label=oYW56
                     /note="LA-BamHI-PPT-MluI-bGH pA PCR"
     primer_bind     complement(3208..3225)
                     /label=oYW53
                     /note="LA-BamHI-bGH pA"
     primer_bind     complement(3209..3226)
                     /label=oYW282
                     /note="Arm-PCR pYW134-R"
     primer_bind     3226..3254
                     /label=oYW189
                     /note="LA-P_CMV-F"
     primer_bind     3245..3262
                     /label=oYW90
                     /note="LA-AgeI-PBS-PCMV F"
     primer_bind     3246..3271
                     /label=oYW355
                     /note="LA-NruI-PEF1a PCR-F"
     primer_bind     3254..3270
                     /label=oYW326
     primer_bind     3274..3298
                     /label=oDJ684-1
     primer_bind     3274..3295
                     /label=oDJ223-1
     primer_bind     3274..3292
                     /label=oYW278
                     /note="LA-NotI-P_GFAP-F"
     primer_bind     3274..3292
                     /label=oYW181
                     /note="P_hCMV-F"
     primer_bind     3276..3315
                     /label=oDJ655-1
     misc_feature    complement(3276..3277)
                     /label=P_RPBSA
                     /label=nonstandard type: promoter_const
     misc_feature    3292
                     /label=CMV
     misc_feature    3313..3330
                     /label=NFAT_IL4
     misc_feature    3343..3360
                     /label=NFAT_IL4
     misc_feature    3373..3390
                     /label=NFAT_IL4
     misc_feature    3403..3420
                     /label=NFAT_IL4
     misc_feature    3433..3450
                     /label=NFAT_IL4
     promoter        3464..3495
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     primer_bind     complement(3499..3547)
                     /label=oDJ684-2
     primer_bind     3528..3560
                     /label=oQZ280
     primer_bind     3528..3549
                     /label=oDJ684-3
     primer_bind     3528..3545
                     /label=oQZ321
     regulatory      3528..3536
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3534..3602
                     /codon_start=1
                     /label=Secretory (IgK)
                     /label=nonstandard type:  signal
                     /translation="MTSETDTLLLWVLLLWVPGSTGD"
     primer_bind     3534..3560
                     /label=oQZ331
     CDS             3534..3536
                     /codon_start=1
                     /label=mGreenLantern
                     /translation="M"
     primer_bind     3537..3558
                     /label=oQZ757
     primer_bind     complement(3604..3636)
                     /label=oQZ758
     primer_bind     3604..3636
                     /label=oQZ759
     CDS             3621..4136
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     complement(3731..3754)
                     /label=oQZ826
     primer_bind     3755..3779
                     /label=oQZ827
     primer_bind     complement(4115..4156)
                     /label=oDJ684-4
     primer_bind     complement(4115..4136)
                     /label=oQZ281
     primer_bind     complement(4115..4136)
                     /label=oDJ657-2
     primer_bind     complement(4118..4136)
                     /label=oQZ322
     primer_bind     complement(4156..4173)
                     /label=BGH/pcDNA3.1R
     primer_bind     complement(4156..4173)
                     /label=pcDNA3.1-R(BGH-R)
     primer_bind     4159..4178
                     /label=oYW188
                     /note="bGH poly A"
     polyA_signal    4162..4386
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4166..4183)
                     /label=oYW190
                     /note="BGH poly A R"
     primer_bind     complement(4228..4246)
                     /label=oYW303
                     /note="PolyA tail-bGH pA"
     primer_bind     complement(4228..4246)
                     /label=oYW57
                     /note="bGH seq primer R"
     primer_bind     complement(4231..4258)
                     /label=oYW300
                     /note="PolyA tail-bGH pA"
     primer_bind     complement(4231..4254)
                     /label=oYW302
                     /note="bGH pA R"
     primer_bind     4231..4250
                     /label=W2F0627-P099-D03
                     /note="Sequencing primer"
     primer_bind     4289..4308
                     /label=BGH-polyA-R
     primer_bind     4369..4387
                     /label=oYW203
                     /note="LA-P_CMV-F"
     primer_bind     complement(4369..4387)
                     /label=oYW202
                     /note="BGH polyA-R"
     primer_bind     complement(4369..4386)
                     /label=oYW56
                     /note="LA-BamHI-PPT-MluI-bGH pA PCR"
     primer_bind     complement(4369..4386)
                     /label=oYW53
                     /note="LA-BamHI-bGH pA"
     primer_bind     complement(4370..4387)
                     /label=oYW282
                     /note="Arm-PCR pYW134-R"
     primer_bind     4397..4413
                     /label=oYW183
                     /note="P_hEF1a PCR F"
     primer_bind     4398..4419
                     /label=oYW197
                     /note="ITR PCR F1"
     primer_bind     complement(4429..4444)
                     /label=oYW336
     misc_feature    complement(4470..4498)
                     /label=SleepingBeauty-ITR_Ri
                     /label=nonstandard type:  ITR
     misc_feature    4664..4695
                     /label=SleepingBeauty-ITR-Ro
                     /label=nonstandard type:  ITR
     primer_bind     complement(4838..4857)
                     /label=oYW207
                     /note="pLL3.7 PCR R"
     primer_bind     complement(4839..4864)
                     /label=oYW301
                     /note="PolyA tail-pLL3.7 3'LTR"
     rep_origin      complement(4883..5471)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5022..5041)
                     /label=W5CF0703-P099-D09
                     /note="Sequencing primer"
     CDS             complement(5642..6502)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5659..5678)
                     /label=W4CF0701-P099-E08
                     /note="Sequencing primer"
     primer_bind     complement(6323..6342)
                     /label=W3CF0630-P099-D08
                     /note="Sequencing primer"
     primer_bind     complement(6379..6398)
                     /label=W3CF0630-P099-A08
                     /note="Sequencing primer"
     primer_bind     complement(6379..6398)
                     /label=W3CF0630-P099-F07
                     /note="Sequencing primer"
     primer_bind     6473..6492
                     /label=oYW24
                     /note="Forward seq primer of AmpR"
     promoter        complement(join(6503..6532,1..75))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
       61 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gatgcggtgt gaaataccgc
      121 acagatgcgt aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa
      181 attggatccc tatacagttg aagtcggaag tttacataca cttaagttgg agtcattaaa
      241 actcgttttt caactactcc acaaatttct tgttaacaaa caatagtttt ggcaagtcag
      301 ttaggacatc tactttgtgc atgacacaag tcatttttcc aacaattgtt tacagacaga
      361 ttatttcact tataattcac tgtatcacaa ttccagtggg tcagaagttt acatacacta
      421 agttcgactc ctctgcagaa tgcggcgatg tttcggtaag gggtccgcta gaattcgaaa
      481 ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
      541 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
      601 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
      661 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
      721 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
      781 gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
      841 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
      901 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
      961 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
     1021 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg
     1081 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
     1141 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
     1201 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc
     1261 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc
     1321 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa
     1381 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc
     1441 aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg
     1501 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg
     1561 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc
     1621 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg aaccggttta
     1681 attaagatat ctgcatctag ggcggccaat tccgcccctc tcccccccgc ccctctccct
     1741 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     1801 atatgtgatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     1861 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     1921 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     1981 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     2041 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     2101 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     2161 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     2221 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     2281 tcctttgaaa aacacgatga taagcttgcc acaacccaca aggagacgac cttccgtcga
     2341 cgccaccatg ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     2401 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     2461 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     2521 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     2581 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     2641 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     2701 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     2761 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     2821 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     2881 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     2941 cccggtgcct gattcgaagg cctgtcgtgg gggatcaatt cgctcgctga tcagcctcga
     3001 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     3061 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     3121 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     3181 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcctag gagaatctgc
     3241 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtggtacct
     3301 gagctcgcta gctacattgg aaaattttat acacgttcta gctacattgg aaaattttat
     3361 acacgttcta gctacattgg aaaattttat acacgttcta gctacattgg aaaattttat
     3421 acacgttcta gctacattgg aaaattttat acacgttaga ctctagaggg tatataatgg
     3481 aagctcgact tccagcttgg caatccggta ctgttggtaa agaattcgcc accatgacta
     3541 gtgagacaga cacactcctg ctatgggtac tgctgctctg ggttccaggt tccactggtg
     3601 acgctagtgg tggttctggt atggtcttca cactcgaaga tttcgttggg gactggcgac
     3661 agacagccgg ctacaacctg gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc
     3721 agaatctcgg ggtgtccgta actccgatcc aaaggattgt cctgagcggt gaaaatgggc
     3781 tgaagatcga catccatgtc atcatcccgt atgaaggtct gagcggcgac caaatgggcc
     3841 agatcgaaaa aatttttaag gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc
     3901 tgcactatgg cacactggta atcgacgggg ttacgccgaa catgatcgac tatttcggac
     3961 ggccgtatga aggcatcgcc gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt
     4021 ggaacggcaa caaaattatc gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc
     4081 gagtaaccat caacggagtg accggctggc ggctgtgcga acgcattctg gcgtgatcta
     4141 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     4201 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     4261 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     4321 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     4381 ctatggcttc tgaggcggaa agaaccagct gggcctacta gctactcggg accccttacc
     4441 gaaacatcgc cgcattctgc agaggagtcg agtgtatgta aacttctgac ccactgggaa
     4501 tgtgatgaaa gaaataaaag ctgaaatgaa tcattctctc tactattatt ctgatatttc
     4561 acattcttaa aataaagtgg tgatcctaac tgacctaaga cagggaattt ttactaggat
     4621 taaatgtcag gaattgtgaa aaagtgagtt taaatgtatt tggctaaggt gtatgtaaac
     4681 ttccgacttc aactgtatag ggatccgctt cctcgctcac tgactcgctg cgctcggtcg
     4741 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     4801 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     4861 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4921 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4981 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     5041 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     5101 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     5161 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     5221 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     5281 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     5341 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     5401 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     5461 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     5521 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     5581 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     5641 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     5701 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     5761 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     5821 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     5881 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     5941 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6001 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     6061 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     6121 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     6181 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     6241 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     6301 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     6361 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     6421 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     6481 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc at
//