| 质粒名称: | pSL320 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002415 |
| 产品用途: | Mammalian PhCMV-driven expression vector for MCP-specific EGFP-mRNA containing 8 tandem MS2-box repeats in the 5’-UTR (PhCMV-(MS2)8-EGFP-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | PhCMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | EGFP |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Rationally designed light-inducible RNA-releasing protein for translational regulation and optogenetic control of gene therapies
NA
NA
|
pSL320
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL320载体质粒基本信息
pSL320质粒图谱载体图谱和pSL320载体序列质粒序列多克隆位点信息
pSL320质粒载体简介
pSL320质粒序列载体序列
LOCUS pSL320 6634 bp DNA circular SYN 05-MAR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6634)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Aug 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6634
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 863..882
/label=T7
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 901..930
/label=hACTB
misc_feature 957..986
/label=hACTB
primer_bind 976..1003
/label=oSCL666
misc_feature 1004..1007
/label=linker
primer_bind complement(1009..1023)
/label=Oscl28-2
primer_bind complement(1010..1023)
/label=8x R
misc_feature 1024..1036
/label=linker
misc_feature 1042..1060
/label=MS2 box
misc_feature 1066..1075
/label=linker
misc_feature 1081..1099
/label=MS2 box
misc_feature 1105..1114
/label=linker
misc_feature 1105..1114
/label=linker
misc_feature 1113..1143
/label=hairpin
misc_feature 1119..1137
/label=MS2 box
misc_feature 1142..1154
/label=linker
misc_feature 1153..1183
/label=hairpin
misc_feature 1159..1177
/label=MS2 box
misc_feature 1187..1192
/label=linker
primer_bind 1200..1218
/label=oSCL2107
misc_feature 1201..1216
/label=linker
misc_feature 1243..1255
/label=linker
misc_feature 1261..1279
/label=MS2 box
misc_feature 1285..1294
/label=linker
misc_feature 1300..1318
/label=MS2 box
misc_feature 1324..1333
/label=linker
misc_feature 1324..1333
/label=linker
misc_feature 1332..1362
/label=hairpin
misc_feature 1338..1356
/label=MS2 box
misc_feature 1361..1373
/label=linker
misc_feature 1372..1402
/label=hairpin
misc_feature 1378..1396
/label=MS2 box
misc_feature 1406..1411
/label=linker
primer_bind 1419..1488
/label=oSCL2107
misc_feature 1420..1435
/label=linker
primer_bind 1436..1460
/label=oSCL666
misc_feature 1441..2197
/label=EGFP
/note="note=""Fragment extracted from OWS233-Kpn1-EGFP-Xba1
[758 nt] : (KpnI[4] / XbaI[766])"
primer_bind 1469..1488
/label=Oscl9-1
/note="for primer of EGFP"
regulatory 1471..1480
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1477..2196
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 1477..2193
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 1480..2193
/label=EGFP
primer_bind complement(2175..2196)
/label=Oscl9-2
primer_bind complement(2175..2196)
/label=Primer 2
primer_bind complement(2198..2217)
/label=oSCL667
terminator 2231..2458
/label=bGH PA
polyA_signal 2234..2458
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2504..2932
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2946..3275
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3126..3261
/label=SV40 ori
/note="SV40 origin of replication"
misc_feature 3126..3203
/label=SV40 ORI
CDS 3342..4136
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 3345..4136
/label=Kan/neoR
polyA_signal 4310..4431
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4476..4496)
/label=M13-rev
primer_bind complement(4480..4496)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_binding complement(4502..4524)
/label=LacO
protein_bind 4504..4520
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4528..4558)
/label=lac promoter
/note="promoter for the E. coli lac operon"
promoter complement(4529..4558)
/label=lac
protein_bind 4573..4594
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4882..5467)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5638..6498)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
CDS complement(5641..6300)
/label=AmpR
promoter complement(6499..6603)
/gene="bla"
/label=AmpR promoter
promoter complement(6540..6568)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gctccatcct ggcctcgctg tccaccttcc aattggagac gagtccgtct cactaggctc
961 catcctggcc tcgctgtcca ccttcctagc ttggtacgga tccaacttga accggtaaga
1021 attgcaacaa acaaatcacc accgtgagga tcacccacgg tggtgaaagc cttagcctag
1081 ccgtgaggat cacccacggt aggcacaaga aattctaccc gtgaggatca cccacgggta
1141 gaatcacaaa gactacagcc gtgaggatca cccacggctg tagactcaaa cttaaaaagg
1201 atatcttcag caaactgatc ggagacgagt ccgtctcaaa ttgcaacaaa caaatcacca
1261 ccgtgaggat cacccacggt ggtgaaagcc ttagcctagc cgtgaggatc acccacggta
1321 ggcacaagaa attctacccg tgaggatcac ccacgggtag aatcacaaag actacagccg
1381 tgaggatcac ccacggctgt agactcaaac ttaaaaagga tatcttcagc aaactgaatt
1441 cagagtttga agtggtgggc cgcagtgttt gccaccatgg tgagcaaggg cgaggagctg
1501 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
1561 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
1621 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
1681 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
1741 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
1801 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
1861 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
1921 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
1981 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
2041 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
2101 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
2161 gggatcactc tcggcatgga cgagctgtac aagtaatcta gagggcccgt ttaaacccgc
2221 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
2281 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
2341 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
2401 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
2461 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
2521 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
2581 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
2641 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
2701 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
2761 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
2821 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
2881 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
2941 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
3001 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
3061 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
3121 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
3181 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
3241 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
3301 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
3361 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
3421 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
3481 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
3541 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
3601 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
3661 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
3721 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
3781 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
3841 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
3901 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
3961 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
4021 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
4081 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
4141 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
4201 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
4261 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
4321 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
4381 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
4441 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
4501 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
4561 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
4621 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
4681 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
4741 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
4801 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
4861 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
4921 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
4981 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
5041 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
5101 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
5161 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
5221 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
5281 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
5341 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
5401 aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
5461 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
5521 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
5581 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
5641 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
5701 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
5761 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
5821 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
5881 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
5941 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
6001 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
6061 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
6121 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
6181 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
6241 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
6301 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
6361 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
6421 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
6481 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
6541 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
6601 gcgcacattt ccccgaaaag tgccacctga cgtc
//
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