| 质粒名称: | pAAVLINK-EFS-HA-WDFY3-A |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001940 |
| 产品用途: | 5' AAVLINK plasmid for WDFY3 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EFS |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | WDFY3 |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EFS-HA-WDFY3-A
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EFS-HA-WDFY3-A载体质粒基本信息
pAAVLINK-EFS-HA-WDFY3-A质粒图谱载体图谱和pAAVLINK-EFS-HA-WDFY3-A载体序列质粒序列多克隆位点信息
pAAVLINK-EFS-HA-WDFY3-A质粒载体简介
pAAVLINK-EFS-HA-WDFY3-A质粒序列载体序列
LOCUS pAAVLINK-EFS-HA-WDFY3-A 7182 bp DNA circular SYN 10-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7182)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 17, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7182
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
promoter 145..356
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
CDS 371..397
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 404..4322
/codon_start=1
/gene="WDFY3"
/gene_synonym="ALFY; BCHS; MCPH18; ZFYVE25"
/product="WD repeat and FYVE domain-containing protein 3"
/label=WDFY3
/note="autophagy-linked FYVE protein"
/db_xref="GI:31317272"
/db_xref="CCDS:CCDS3609.1"
/db_xref="GeneID:23001"
/db_xref="HGNC:HGNC:20751"
/db_xref="MIM:617485"
/protein_id="NP_055806.2"
/translation="NMVKRIMGRPRQEECSPQDNALGLMHLRRLFTELCHPPRHMTQKE
QEEKLYMMLPVFNRVFGNAPPNTMTEKFSDLLQFTTQVSRLMVTEIRRRASNKSTEAAS
RAIVQFLEINQSEEASRGWMLLTTINLLASSGQKTVDCMTTMSVPSTLVKCLYLFFDLP
HVPEAVGGAQNELPLAERRGLLQKVFVQILVKLCSFVSPAEELAQKDDLQLLFSAITSW
CPPYNLPWRKSAGEVLMTISRHGLSVNVVKYIHEKECLSTCVQNMQQSDDLSPLEIVEM
FAGLSCFLKDSSDVSQTLLDDFRIWQGYNFLCDLLLRLEQAKEAESKDALKDLVNLITS
LTTYGVSELKPAGITTGAPFLLPGFAVPQPAGKGHSVRNVQAFAVLQNAFLKAKTSFLA
QIILDAITNIYMADNANYFILESQHTLSQFAEKISKLPEVQNKYFEMLEFVVFSLNYIP
CKELISVSILLKSSSSYHCSIIAMKTLLKFTRHDYIFKDVFREVGLLEVMVNLLHKYAA
LLKDPTQALNEQGDSRNNSSVEDQKHLALLVMETLTVLLQGSNTNAGIFREFGGARCAH
NIVKYPQCRQHALMTIQQLVLSPNGDDDMGTLLGLMHSAPPTELQLKTDILRALLSVLR
ESHRSRTVFRKVGGFVYITSLLVAMERSLSCPPKNGWEKVNQNQVFELLHTVFCTLTAA
MRYEPANSHFFKTEIQYEKLADAVRFLGCFSDLRKISAMNVFPSNTQPFQRLLEEDVIS
IESVSPTLRHCSKLFIYLYKVATDSFDSRAEQIPPCLTSESSLPSPWGTPALSRKRHAY
HSVSTPPVYPPKNVADLKLHVTTSSLQSSDAVIIHPGAMLAMLDLLASVGSVTQPEHAL
DLQLAVANILQSLVHTERNQQVMCEAGLHARLLQRCSAALADEDHSLHPPLQRMFERLA
SQALEPMVLREFLRLASPLNCGAWDKKLLKQYRVHKPSSLSYEPEMRSSMITSLEGLGT
DNVFSLHEDNHYRISKSLVKSAEGSTVPLTRVKCLVSMTTPHDIRLHGSSVTPAFVEFD
TSLEGFGCLFLPSLAPHNAPTNNTVTTGLIDGAVVSGIGSGERFFPPPSGLSYSSWFCI
EHFSSPPNNHPVRLLTVVRRANSSEQHYVCLAIVLSAKDRSLIVSTKEELLQNYVDDFS
EESSFYEILPCCARFRCGELIIEGQWHHLVLVMSKGMLKNSTAALYIDGQLVNTVKLHY
VHSTPGGSGSANPPVVSTVYAYIGTPPAQRQIASLVWRLGPTHFLEEVLPSSNVTTIYE
LGPNYVGSFQAVCMPCKDAKSE"
misc_feature 438..443
/label=SDS
misc_feature 2290..2295
/label=SDS
misc_feature 2762..2767
/label=SDS
misc_feature 2867..2872
/label=SDS
misc_feature 3683..3688
/label=SDS
intron 4323..4363
/label=Splicing Donor
misc_feature 4367..4400
/label=loxP-JT1571
repeat_region 4445..4585
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4445..4574
/label=AAV2 ITR
repeat_region 4446..4586
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4660..5115
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5397..5501
/gene="bla"
/label=AmpR promoter
CDS 5502..6362
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6533..7121
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgggcag agcgcacatc gcccacagtc cccgagaagt
181 tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg
241 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
301 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagcgta
361 cgccaccatg tacccttatg acgtacctga ctatgctgga tctaacatgg tgaagaggat
421 catggggcgg ccgaggcagg aggagtgcag cccacaagac aacgccttag gactgatgca
481 cctccgccgg ctcttcacgg agttgtgcca tcctccccgg cacatgactc agaaggaaca
541 agaagagaaa ctgtatatga tgctgccagt gtttaacagg gtttttggaa atgctccgcc
601 gaatacaatg acagaaaaat tttctgatct tctgcagttc acaacacaag tctcacgact
661 aatggtgaca gaaattcgaa ggagagcatc aaacaaatcc acagaggctg caagtcgggc
721 catagttcag ttcctagaga ttaatcagag tgaagaagcc agtagaggct ggatgcttct
781 aacgacaatt aatttgttag cttcctctgg tcagaaaacc gtggactgca tgacaacaat
841 gtcagtgcct tccaccctgg ttaaatgttt atatctgttt tttgaccttc cacatgtgcc
901 tgaggcagtt ggaggtgcac agaatgagct acctctagca gaacgtcgag gactactcca
961 gaaagttttt gtacagatct tagtgaaact gtgcagtttt gtttcccctg cggaggagct
1021 ggctcagaaa gatgatctcc agcttctatt cagtgcaata acctcttggt gccctcccta
1081 taacctgcct tggagaaaga gtgctggaga agtcctcatg accatatctc gtcatggtct
1141 tagtgtcaat gtagtgaagt atattcatga gaaagagtgt ttatctacat gtgttcagaa
1201 tatgcagcaa tcagatgacc tgtctcccct agaaattgtc gaaatgtttg ctgggctttc
1261 ttgtttcctc aaagattcca gcgatgtttc ccaaacactt ctggatgatt ttcggatatg
1321 gcaaggatat aattttcttt gtgatctctt gcttagattg gaacaagcaa aagaggcaga
1381 atccaaagat gccttgaaag atctggttaa tctgataact tccctaacaa catatggtgt
1441 cagtgaacta aaaccagctg gtattaccac aggggcaccc tttttattgc ctggatttgc
1501 agtacctcag cctgcaggca aaggtcacag tgtgagaaac gtccaggcct ttgcagttct
1561 tcagaatgca tttttaaaag caaaaaccag cttccttgcc caaatcatcc ttgatgctat
1621 cacaaatatt tacatggctg acaatgccaa ttacttcatc ctagagtcac agcacacatt
1681 gtcacagttt gcagagaaga tttctaaact cccagaagta caaaacaaat actttgagat
1741 gctggagttt gttgttttta gcttaaatta tataccttgt aaagaactta ttagtgtcag
1801 tatcctctta aaatctagct cttcttatca ctgtagcatt attgcaatga aaacacttct
1861 taagtttaca agacatgact acatatttaa agacgtgttc agggaggttg gccttttgga
1921 ggtcatggta aaccttttgc ataaatatgc tgccctgttg aaggatccaa ctcaggcact
1981 aaatgaacaa ggggactcaa gaaataatag ttcagttgaa gaccaaaaac acctggcttt
2041 attggttatg gagaccttga cagtgcttct tcaaggatca aacacaaatg caggaatttt
2101 tcgagaattt ggaggtgcaa gatgtgcaca taatatagta aagtaccctc aatgccggca
2161 gcatgccttg atgactatcc aacagctggt gctctcccca aatggggacg atgacatggg
2221 cactctcctg gggctaatgc attcagcccc accgacggaa ttgcagttga agactgatat
2281 tttaagggcc ctcctgtcgg tccttcgaga aagccatcgt tcaagaacag tttttaggaa
2341 agttggagga tttgtgtaca ttacatcctt gctcgttgct atggaaagat ctttgagctg
2401 tccacccaag aatggctggg agaaagtgaa ccagaatcaa gtgtttgaac ttcttcacac
2461 tgtgttctgc acgttgactg cagcaatgcg ctatgagcca gccaactctc atttcttcaa
2521 aacagagatt cagtatgaga agttggcaga tgctgttcga tttcttggct gcttctcaga
2581 cctaagaaaa ataagcgcca tgaatgtctt cccctcaaat acacagccat ttcaaagact
2641 tttagaggaa gatgtaatct caatagaatc agtgtcaccc acgttacggc actgcagtaa
2701 actttttatt tatctttaca aagtagccac agattctttt gacagtcgtg cagaacagat
2761 ccctccttgc ctgacaagtg agtcttctct cccctctcct tggggtacac cagctttgtc
2821 caggaaaagg catgcatatc attctgtttc aactccccct gtttaccctc ctaaaaatgt
2881 tgccgacctg aaactacatg tgacaacttc atctctgcag agttctgatg cagtcatcat
2941 tcatcctgga gccatgcttg ccatgctgga cctactggcc tctgttgggt cagtgacaca
3001 gccagaacat gctttggatc ttcaacttgc cgtggcaaat attttacaat ccctggtgca
3061 cacagaaagg aaccagcaag tcatgtgtga agctggtctt catgcacgac tgctgcagag
3121 gtgcagtgct gcattggctg atgaggacca ctcactgcac ccgcccctgc agcggatgtt
3181 tgaacgatta gcctctcagg ctctggaacc catggtgttg agggagtttt tacgtttggc
3241 aagtccttta aattgtggtg cctgggacaa aaaactgcta aaacaatata gggtccacaa
3301 accaagttca ctgagttatg aaccagaaat gagaagtagt atgatcacat ctctggaagg
3361 tctgggtact gataatgttt ttagcttaca tgaagataac cattaccgga taagcaagag
3421 cctggtaaaa tctgcggaag gaagtactgt acccctgacc agggtgaagt gtctggtctc
3481 catgacaacc ccacatgaca tcagacttca tgggtcatca gttactccag cttttgttga
3541 atttgacaca tcacttgaag ggtttggatg tctttttttg cccagtttgg cccctcataa
3601 tgctcctaca aataataccg tcacaacagg tcttattgat ggggctgtgg tcagtggcat
3661 tggttctggt gaaagattct tccctcctcc ctccggctta agttactcta gctggttttg
3721 tattgaacat tttagttctc ctccaaataa ccaccctgtc agacttctta ctgttgtgcg
3781 ccgagcaaat tcttctgagc aacattacgt gtgccttgca atagttctat cagcaaaaga
3841 ccgatctctg attgtttcca ccaaagagga actcctccaa aattatgttg atgattttag
3901 tgaagagtcc tcattttatg aaattctccc atgctgtgct cgctttcgat gtggagagct
3961 tatcattgag ggacagtggc atcatttggt cctggtaatg agcaaaggca tgttgaaaaa
4021 cagtactgca gccctttata ttgatggaca gcttgttaac actgtaaagc ttcattatgt
4081 ccacagtact ccagggggtt caggttcggc aaatccacca gtggtgagca cggtctatgc
4141 ctacattggt actccacctg cccaacgcca aattgcctca ttggtttggc gcctgggacc
4201 cacacatttt ctagaagaag ttttaccttc ttcaaatgtt actaccattt atgaacttgg
4261 accaaattat gttggaagct ttcaggctgt atgtatgcca tgtaaagatg caaaatccga
4321 aggtaagtat caaggttaca agacaggttt aaggagacca ataagctatt attcgtatag
4381 catacattat acgaagttat taagttatgg agaccggtgt ggcacgtgcg gaccgagcgg
4441 ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact
4501 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc
4561 gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg
4621 tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat
4681 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag
4741 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
4801 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
4861 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
4921 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
4981 caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
5041 tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
5101 taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
5161 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
5221 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
5281 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
5341 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
5401 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
5461 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
5521 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
5581 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
5641 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
5701 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
5761 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
5821 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
5881 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
5941 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
6001 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
6061 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
6121 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
6181 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
6241 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
6301 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
6361 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
6421 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
6481 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
6541 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
6601 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
6661 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
6721 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
6781 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
6841 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
6901 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
6961 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
7021 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
7081 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
7141 ttttacggtt cctggccttt tgctggcctt ttgctcacat gt
//
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