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pTagRFP-actin载体质粒基本信息
pTagRFP-actin质粒图谱载体图谱和pTagRFP-actin载体序列质粒序列多克隆位点信息
pTagRFP-actin质粒载体简介
pTagRFP-actin质粒序列载体序列
LOCUS pTagRFP-actin 5814 bp DNA circular SYN 12-JUL-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5814)
AUTHORS Biofeng
TITLE Direct Submission
JOURNAL Exported Jul 12, 2023 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5814
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
regulatory 607..616
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 613..1323
/codon_start=1
/product="monomeric derivative of red fluorescent protein
from Entacmaea quadricolor (Merzlyak et al., 2007)"
/label=TagRFP
/note="mammalian codon-optimized"
/translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
ARYCDLPSKLGHKLN"
polyA_signal 2602..2723
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(2730..3185)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3212..3316
/gene="bla"
/label=AmpR promoter
promoter 3318..3675
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3526..3661
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3710..4504
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4736..4783
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5112..5700
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggtgtc taagggcgaa gagctgatta aggagaacat gcacatgaag
661 ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga gggcgaaggc
721 aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc
781 ttcgccttcg acatcctggc taccagcttc atgtacggca gcagaacctt catcaaccac
841 acccagggca tccccgactt ctttaagcag tccttccctg agggcttcac atgggagaga
901 gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac
961 ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa cggccctgtg
1021 atgcagaaga aaacactcgg ctgggaggcc aacaccgaga tgctgtaccc cgctgacggc
1081 ggcctggaag gcagaagcga catggccctg aagctcgtgg gcgggggcca cctgatctgc
1141 aacttcaaga ccacatacag atccaagaaa cccgctaaga acctcaagat gcccggcgtc
1201 tactatgtgg accacagact ggaaagaatc aaggaggccg acaaagagac ctacgtcgag
1261 cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg gcacaaactt
1321 aattccggac tcagatctcg agctatggat gatgatatcg ccgcgctcgt cgtcgacaac
1381 ggctccggca tgtgcaaggc cggcttcgcg ggcgacgatg ccccccgggc cgtcttcccc
1441 tccatcgtgg ggcgccccag gcaccagggc gtgatggtgg gcatgggtca gaaggattcc
1501 tatgtgggcg acgaggccca gagcaagaga ggcatcctca ccctgaagta ccccatcgag
1561 cacggcatcg tcaccaactg ggacgacatg gagaaaatct ggcaccacac cttctacaat
1621 gagctgcgtg tggctcccga ggagcacccc gtgctgctga ccgaggcccc cctgaacccc
1681 aaggccaacc gcgagaagat gacccagatc atgtttgaga ccttcaacac cccagccatg
1741 tacgttgcta tccaggctgt gctatccctg tacgcctctg gccgtaccac tggcatcgtg
1801 atggactccg gtgacggggt cacccacact gtgcccatct acgaggggta tgccctcccc
1861 catgccatcc tgcgtctgga cctggctggc cgggacctga ctgactacct catgaagatc
1921 ctcaccgagc gcggctacag cttcaccacc acggccgagc gggaaatcgt gcgtgacatt
1981 aaggagaagc tgtgctacgt cgccctggac ttcgagcaag agatggccac ggctgcttcc
2041 agctcctccc tggagaagag ctacgagctg cctgacggcc aggtcatcac cattggcaat
2101 gagcggttcc gctgccctga ggcactcttc cagccttcct tcctgggcat ggagtcctgt
2161 ggcatccacg aaactacctt caactccatc atgaagtgtg acgtggacat ccgcaaagac
2221 ctgtacgcca acacagtgct gtctggcggc accaccatgt accctggcat tgccgacagg
2281 atgcagaagg agatcactgc cctggcaccc agcacaatga agatcaagat cattgctcct
2341 cctgagcgca agtactccgt gtggatcggc ggctccatcc tggcctcgct gtccaccttc
2401 cagcagatgt ggatcagcaa gcaggagtat gacgagtccg gcccctccat cgtccaccgc
2461 aaatgcttct agggatccac cggatctaga taactgatca taatcagcca taccacattt
2521 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
2581 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
2641 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
2701 tccaaactca tcaatgtatc ttaacgcgta aattgtaagc gttaatattt tgttaaaatt
2761 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
2821 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
2881 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
2941 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
3001 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
3061 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
3121 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
3181 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
3241 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
3301 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
3361 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
3421 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
3481 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
3541 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
3601 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
3661 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca
3721 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
3781 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
3841 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
3901 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
3961 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
4021 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
4081 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
4141 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
4201 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
4261 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
4321 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
4381 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
4441 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
4501 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
4561 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
4621 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg
4681 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
4741 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg
4801 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
4861 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
4921 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat
4981 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
5041 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
5101 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
5161 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
5221 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
5281 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
5341 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
5401 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
5461 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
5521 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
5581 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
5641 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
5701 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
5761 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat
//
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