| 质粒名称: | pAAVLINK-EFS-HA-5'-TET2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001883 |
| 产品用途: | 5' AAVLINK plasmid for TET2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: |
EFS
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | TET2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EFS-HA-5'-TET2
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EFS-HA-5'-TET2载体质粒基本信息
pAAVLINK-EFS-HA-5'-TET2质粒图谱载体图谱和pAAVLINK-EFS-HA-5'-TET2载体序列质粒序列多克隆位点信息
pAAVLINK-EFS-HA-5'-TET2质粒载体简介
pAAVLINK-EFS-HA-5'-TET2质粒序列载体序列
LOCUS pAAVLINK-EFS-HA-5'-TET2 7086 bp DNA circular SYN 10-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7086)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7086
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
promoter 145..356
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
CDS 372..398
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 405..4226
/codon_start=1
/gene="TET2"
/gene_synonym="IMD75; KIAA1546; MDS"
/product="methylcytosine dioxygenase TET2 isoform a"
/EC_number="1.14.11.80"
/label=TET2
/note="isoform a is encoded by transcript variant
1;probable methylcytosine dioxygenase TET2; tet oncogene
family member 2; ten-eleven translocation 2"
/db_xref="GI:187761317"
/db_xref="CCDS:CCDS47120.1"
/db_xref="GeneID:54790"
/db_xref="HGNC:HGNC:25941"
/db_xref="MIM:612839"
/protein_id="NP_001120680.1"
/translation="MEQDRTNHVEGNRLSPFLIPSPPICQTEPLATKLQNGSPLPERAH
PEVNGDTKWHSFKSYYGIPCMKGSQNSRVSPDFTQESRGYSKCLQNGGIKRTVSEPSLS
GLLQIKKLKQDQKANGERRNFGVSQERNPGESSQPNVSDLSDKKESVSSVAQENAVKDF
TSFSTHNCSGPENPELQILNEQEGKSANYHDKNIVLLKNKAVLMPNGATVSASSVEHTH
GELLEKTLSQYYPDCVSIAVQKTTSHINAINSQATNELSCEITHPSHTSGQINSAQTSN
SELPPKPAAVVSEACDADDADNASKLAAMLNTCSFQKPEQLQQQKSVFEICPSPAENNI
QGTTKLASGEEFCSGSSSNLQAPGGSSERYLKQNEMNGAYFKQSSVFTKDSFSATTTPP
PPSQLLLSPPPPLPQVPQLPSEGKSTLNGGVLEEHHHYPNQSNTTLLREVKIEGKPEAP
PSQSPNPSTHVCSPSPMLSERPQNNCVNRNDIQTAGTMTVPLCSEKTRPMSEHLKHNPP
IFGSSGELQDNCQQLMRNKEQEILKGRDKEQTRDLVPPTQHYLKPGWIELKAPRFHQAE
SHLKRNEASLPSILQYQPNLSNQMTSKQYTGNSNMPGGLPRQAYTQKTTQLEHKSQMYQ
VEMNQGQSQGTVDQHLQFQKPSHQVHFSKTDHLPKAHVQSLCGTRFHFQQRADSQTEKL
MSPVLKQHLNQQASETEPFSNSHLLQHKPHKQAAQTQPSQSSHLPQNQQQQQKLQIKNK
EEILQTFPHPQSNNDQQREGSFFGQTKVEECFHGENQYSKSSEFETHNVQMGLEEVQNI
NRRNSPYSQTMKSSACKIQVSCSNNTHLVSENKEQTTHPELFAGNKTQNLHHMQYFPNN
VIPKQDLLHRCFQEQEQKSQQASVLQGYKNRNQDMSGQQAAQLAQQRYLIHNHANVFPV
PDQGGSHTQTPPQKDTQKHAALRWHLLQKQEQQQTQQPQTESCHSQMHRPIKVEPGCKP
HACMHTAPPENKTWKKVTKQENPPASCDNVQQKSIIETMEQHLKQFHAKSLFDHKALTL
KSQKQVKVEMSGPVTVLTRQTTAAELDSHTPALEQQTTSSEKTPTKRTAASVLNNFIES
PSKLLDTPIKNLLDTPVKTQYDFPSCRCVEQIIEKDEGPFYTHLGAGPNVAAIREIMEE
RFGQKGKAIRIERVIYTGKEGKSSQGCPIAKWVVRRSSSEEKLLCLVRERAGHTCEAAV
IVILILVWEGIPLSLADKLYSELTETLRKYGTLTNRRCALNEERTCACQ"
misc_feature 958..963
/label=SDS
CDS 1594..1611
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
misc_feature 1629..1634
/label=SDS
misc_feature 1632..1637
/label=SDS
intron 4227..4267
/label=Splicing Donor
misc_feature 4271..4304
/label=lox2272-JT1571
repeat_region 4349..4489
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4349..4478
/label=AAV2 ITR
repeat_region 4350..4490
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4564..5019
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5301..5405
/gene="bla"
/label=AmpR promoter
CDS 5406..6266
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6437..7025
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgggcag agcgcacatc gcccacagtc cccgagaagt
181 tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg
241 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
301 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagcgta
361 cggccaccat gtacccttat gacgtacctg actatgctgg atctatggaa caggatagaa
421 ccaaccatgt tgagggcaac agactaagtc cattcctgat accatcacct cccatttgcc
481 agacagaacc tctggctaca aagctccaga atggaagccc actgcctgag agagctcatc
541 cagaagtaaa tggagacacc aagtggcact ctttcaaaag ttattatgga ataccctgta
601 tgaagggaag ccagaatagt cgtgtgagtc ctgactttac acaagaaagt agagggtatt
661 ccaagtgttt gcaaaatgga ggaataaaac gcacagttag tgaaccttct ctctctgggc
721 tccttcagat caagaaattg aaacaagacc aaaaggctaa tggagaaaga cgtaacttcg
781 gggtaagcca agaaagaaat ccaggtgaaa gcagtcaacc aaatgtctcc gatttgagtg
841 ataagaaaga atctgtgagt tctgtagccc aagaaaatgc agttaaagat ttcaccagtt
901 tttcaacaca taactgcagt gggcctgaaa atccagagct tcagattctg aatgagcagg
961 aggggaaaag tgctaattac catgacaaga acattgtatt acttaaaaac aaggcagtgc
1021 taatgcctaa tggtgctaca gtttctgcct cttccgtgga acacacacat ggtgaactcc
1081 tggaaaaaac actgtctcaa tattatccag attgtgtttc cattgcggtg cagaaaacca
1141 catctcacat aaatgccatt aacagtcagg ctactaatga gttgtcctgt gagatcactc
1201 acccatcgca tacctcaggg cagatcaatt ccgcacagac ctctaactct gagctgcctc
1261 caaagccagc tgcagtggtg agtgaggcct gtgatgctga tgatgctgat aatgccagta
1321 aactagctgc aatgctaaat acctgttcct ttcagaaacc agaacaacta caacaacaaa
1381 aatcagtttt tgagatatgc ccatctcctg cagaaaataa catccaggga accacaaagc
1441 tagcgtctgg tgaagaattc tgttcaggtt ccagcagcaa tttgcaagct cctggtggca
1501 gctctgaacg gtatttaaaa caaaatgaaa tgaatggtgc ttacttcaag caaagctcag
1561 tgttcactaa ggattccttt tctgccacta ccacaccacc accaccatca caattgcttc
1621 tttctccccc tcctcctctt ccacaggttc ctcagcttcc ttcagaagga aaaagcactc
1681 tgaatggtgg agttttagaa gaacaccacc actaccccaa ccaaagtaac acaacacttt
1741 taagggaagt gaaaatagag ggtaaacctg aggcaccacc ttcccagagt cctaatccat
1801 ctacacatgt atgcagccct tctccgatgc tttctgaaag gcctcagaat aattgtgtga
1861 acaggaatga catacagact gcagggacaa tgactgttcc attgtgttct gagaaaacaa
1921 gaccaatgtc agaacacctc aagcataacc caccaatttt tggtagcagt ggagagctac
1981 aggacaactg ccagcagttg atgagaaaca aagagcaaga gattctgaag ggtcgagaca
2041 aggagcaaac acgagatctt gtgcccccaa cacagcacta tctgaaacca ggatggattg
2101 aattgaaggc ccctcgtttt caccaagcgg aatcccatct aaaacgtaat gaggcatcac
2161 tgccatcaat tcttcagtat caacccaatc tctccaatca aatgacctcc aaacaataca
2221 ctggaaattc caacatgcct ggggggctcc caaggcaagc ttacacccag aaaacaacac
2281 agctggagca caagtcacaa atgtaccaag ttgaaatgaa tcaagggcag tcccaaggta
2341 cagtggacca acatctccag ttccaaaaac cctcacacca ggtgcacttc tccaaaacag
2401 accatttacc aaaagctcat gtgcagtcac tgtgtggcac tagatttcat tttcaacaaa
2461 gagcagattc ccaaactgaa aaacttatgt ccccagtgtt gaaacagcac ttgaatcaac
2521 aggcttcaga gactgagcca ttttcaaact cacacctttt gcaacataag cctcataaac
2581 aggcagcaca aacacaacca tcccagagtt cacatctccc tcaaaaccag caacagcagc
2641 aaaaattaca aataaagaat aaagaggaaa tactccagac ttttcctcac ccccaaagca
2701 acaatgatca gcaaagagaa ggatcattct ttggccagac taaagtggaa gaatgttttc
2761 atggtgaaaa tcagtattca aaatcaagcg agttcgagac tcataatgtc caaatgggac
2821 tggaggaagt acagaatata aatcgtagaa attcccctta tagtcagacc atgaaatcaa
2881 gtgcatgcaa aatacaggtt tcttgttcaa acaatacaca cctagtttca gagaataaag
2941 aacagactac acatcctgaa ctttttgcag gaaacaagac ccaaaacttg catcacatgc
3001 aatattttcc aaataatgtg atcccaaagc aagatcttct tcacaggtgc tttcaagaac
3061 aggagcagaa gtcacaacaa gcttcagttc tacagggata taaaaataga aaccaagata
3121 tgtctggtca acaagctgcg caacttgctc agcaaaggta cttgatacat aaccatgcaa
3181 atgtttttcc tgtgcctgac cagggaggaa gtcacactca gacccctccc cagaaggaca
3241 ctcaaaagca tgctgctcta aggtggcatc tcttacagaa gcaagaacag cagcaaacac
3301 agcaacccca aactgagtct tgccatagtc agatgcacag gccaattaag gtggaacctg
3361 gatgcaagcc acatgcctgt atgcacacag caccaccaga aaacaaaaca tggaaaaagg
3421 taactaagca agagaatcca cctgcaagct gtgataatgt gcagcaaaag agcatcattg
3481 agaccatgga gcagcatctg aagcagtttc acgccaagtc gttatttgac cataaggctc
3541 ttactctcaa atcacagaag caagtaaaag ttgaaatgtc agggccagtc acagttttga
3601 ctagacaaac cactgctgca gaacttgata gccacacccc agctttagag cagcaaacaa
3661 cttcttcaga aaagacacca accaaaagaa cagctgcttc tgttctcaat aattttatag
3721 agtcaccttc caaattacta gatactccta taaaaaattt attggataca cctgtcaaga
3781 ctcaatatga tttcccatct tgcagatgtg tagagcaaat tattgaaaaa gatgaaggtc
3841 ctttttatac ccatctagga gcaggtccta atgtggcagc tattagagaa atcatggaag
3901 aaaggtttgg acagaagggt aaagctatta ggattgaaag agtcatctat actggtaaag
3961 aaggcaaaag ttctcaggga tgtcctattg ctaagtgggt ggttcgcaga agcagcagtg
4021 aagagaagct actgtgtttg gtgcgggagc gagctggcca cacctgtgag gctgcagtga
4081 ttgtgattct catcctggtg tgggaaggaa tcccgctgtc tctggctgac aaactctact
4141 cggagcttac cgagacgctg aggaaatacg gcacgctcac caatcgccgg tgtgccttga
4201 atgaagagag aacttgcgcc tgtcaggtaa gtatcaaggt tacaagacag gtttaaggag
4261 accaataagc tattattcgt ataggatact ttatacgaag ttattaagtt atggagaccg
4321 gtgtggcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
4381 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
4441 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
4501 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
4561 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
4621 ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg
4681 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
4741 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
4801 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
4861 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt
4921 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat
4981 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
5041 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
5101 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
5161 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
5221 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
5281 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
5341 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
5401 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
5461 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
5521 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
5581 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
5641 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
5701 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
5761 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
5821 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
5881 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
5941 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
6001 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
6061 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
6121 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
6181 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
6241 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
6301 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
6361 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
6421 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
6481 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
6541 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
6601 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
6661 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
6721 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
6781 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
6841 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
6901 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
6961 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
7021 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
7081 acatgt
//
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pAAVLINK-EFS-HA-5'-TET2质粒载体应用举例
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