VPS13C&mClover3

LOCUS        VPS13C^mClover3               15964 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  VPS13C internally tagged with mClover3 at residue 1914..
ACCESSION   .
VERSION     .
KEYWORDS    VPS13C^mClover3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15964)
  AUTHORS   Kumar N, Leonzino M, Hancock-Cerutti W, Horenkamp FA, Li P, Lees JA,
            Wheeler H, Reinisch KM, De Camilli P
  TITLE     VPS13A and VPS13C are lipid transport proteins differentially 
            localized at ER contact sites.
  JOURNAL   J Cell Biol. 2018 Oct 1;217(10):3625-3639. doi: 
            10.1083/jcb.201807019. Epub 2018 Aug 9.
  PUBMED    30093493
REFERENCE   2  (bases 1 to 15964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15964
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    591..671
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      673..682
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     RBS             3837..3845
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(6463..6484)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     7071..7092
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    12752..12873
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12789..12808)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12843..12862
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(12880..13335)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13017..13038)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13229..13248
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        13362..13466
                     /gene="bla"
                     /label=AmpR promoter
     promoter        13468..13825
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(13491..13511)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      13676..13811
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     13738..13757
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             13860..14654
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13914..13933)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     14524..14543
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(14842..14861)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    14886..14933
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      15262..15850
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15751..15770
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      661 gatccaccgg tcgccaccat ggtgctggag tcggtggtcg cggacttgct gaaccgcttc
      721 ctgggggact atgtggagaa cctgaacaag tcccagctga agctgggcat ctggggcgga
      781 aatgtggctt tagataatct acagataaaa gaaaatgccc tgagtgaatt ggatgttcct
      841 tttaaagtca aggctggcca aattgataaa ttaactttga agattccttg gaagaacctt
      901 tatggagaag cagttgttgc gaccctggaa ggattatacc tgcttgttgt ccctggagca
      961 agtattaagt atgatgctgt aaaagaagaa aaatccttgc aggatgttaa acagaaagag
     1021 ctatcccgaa ttgaagaagc ccttcaaaaa gcagcagaaa aaggcacaca ttcaggggag
     1081 ttcatatatg gcttggagaa ctttgtttac aaggacatca agcctggacg taaacgtaaa
     1141 aagcacaaaa aacattttaa gaaacctttt aaaggtcttg atcgttcaaa agataagcca
     1201 aaagaagcca aaaaggatac atttgtggaa aaattggcaa ctcaagtaat aaaaaatgta
     1261 caagtaaaaa tcacagatat tcacattaaa tatgaagatg atgtcactga tccaaagcgg
     1321 cctctttcat ttggtgtcac actgggagag cttagtctac tgactgcaaa tgaacactgg
     1381 actccatgca tattaaatga agcagacaaa attatataca agcttatacg acttgatagt
     1441 cttagcgcct actggaatgt aaattgcagc atgtcttacc agagatcaag ggaacagatt
     1501 ttggatcagc tgaaaaatga aattcttaca agtggaaata tacccccaaa ttatcaatac
     1561 attttccagc caatatcagc ctctgcaaaa ctctacatga atccttatgc agaatcagag
     1621 ctcaaaacgc ccaaactgga ttgcaacata gaaatacaaa atattgccat tgaactgacc
     1681 aaacctcagt acttaagtat gattgacctt ttggagtcag tggattatat ggttaggaat
     1741 gcgccttata ggaaatacaa gccttattta ccacttcata ccaatggtcg acgatggtgg
     1801 aaatatgcaa ttgattctgt tcttgaagtt catataagaa ggtatacaca gatgtggtca
     1861 tggagtaaca taaaaaagca caggcagtta ctcaagagtt ataaaattgc ctacaaaaac
     1921 aagttaacac agtctaaagt ctcagaagaa atacagaaag aaattcagga cttggagaag
     1981 actctagatg tttttaacat aattttagca aggcaacaag cacaagttga ggtgattcgg
     2041 tctgggcaaa aattaaggaa aaagtctgct gacacaggcg agaaacgtgg aggctggttt
     2101 agtgggttgt ggggtaagaa agagtctaag aaaaaggacg aagaatcatt gattcctgaa
     2161 actattgatg accttatgac tccagaggaa aaagataaac tcttcactgc cattggttat
     2221 agtgagagta cccacaacct aactttacct aagcagtatg ttgcccatat tatgaccctg
     2281 aagttagtaa gcacctctgt tacgataaga gaaaacaaga atattccaga aatactaaaa
     2341 attcagataa ttggcctggg cactcaagta tctcagcgac caggagcaca agcacttaag
     2401 gtagaagcga aattagaaca ctggtatata acaggtttga gacagcagga tattgtgcca
     2461 tcacttgtgg cttcaattgg tgacactaca tcatccttgc ttaaaattaa atttgaaacc
     2521 aatccggagg atagtcctgc tgaccagact ctgattgttc agtcccagcc tgtggaggtc
     2581 atctatgatg ctaaaactgt caatgcagtg gttgaattct ttcaatcaaa taagggattg
     2641 gatcttgagc aaataacatc agcaacattg atgaagctgg aagaaattaa ggagagaaca
     2701 gctacaggac ttacacatat tattgaaact cgaaaagtcc ttgatttaag gataaatctg
     2761 aagccttctt atctagtagt tccacagacg ggtttccacc atgaaaagtc agatcttctg
     2821 attttagatt ttggtacatt tcagctcaac agtaaagatc aaggtttaca gaagactact
     2881 aattcatctc tggaagaaat aatggataag gcatatgaca agtttgatgt tgaaataaaa
     2941 aatgtacaac tactttttgc aagagcagag gaaacctgga aaaagtgtcg atttcagcat
     3001 ccatcaacta tgcatatatt gcaacccatg gatattcatg ttgagttggc taaggccatg
     3061 gtagaaaaag acattagaat ggccagattt aaagtgtcag gaggacttcc tttgatgcat
     3121 gtgagaattt ctgaccagaa gatgaaagat gtgctatatt tgatgaacag tatacctttg
     3181 ccacagaaat catcagccca gtctccagag agacaggtat cctcaattcc tattatttca
     3241 ggtggtacaa aaggtctact tggtacttca ctattgctag acactgtgga atcagagtct
     3301 gatgatgagt attttgatgc tgaagatgga gaaccacaga cttgtaaaag tatgaaagga
     3361 tcagaactta aaaaagctgc agaggtccca aatgaggagc tcatcaatct tctactcaag
     3421 tttgaaatta aagaagtgat tttggaattt actaaacagc agaaagaaga agatacaatt
     3481 ctagtattta atgttactca gttaggaaca gaggccacaa tgagaacatt tgacttaact
     3541 gtggtatctt atttaaagaa aatcagcttg gattatcatg aaattgaagg atccaaaagg
     3601 aagccccttc acttgattag ctcttctgac aaacctggat tagatctttt gaaagtggag
     3661 tatattaagg ctgataagaa tggacctagt tttcaaactg cttttggaaa aactgaacaa
     3721 acagttaagg tggccttttc atctttaaat ctgttgctgc aaacacaagc tcttgtcgct
     3781 tctattaatt acctcacaac cattattcca tctgatgatc aaagcataag tgttgctaag
     3841 gaggtacaaa tttcaactga aaaacaacaa aaaaattcaa ctctgccaaa agcgattgta
     3901 tcctccagag atagtgacat tattgatttc aggctatttg ccaagttgaa tgctttctgt
     3961 gtcattgttt gcaacgaaaa gaacaatatc gccgaaatca agattcaagg actggattcc
     4021 tccctttctc tccagtcaag aaagcagtca ctttttgccc gactagaaaa tattattgtc
     4081 acagatgttg atccaaagac agttcataag aaagctgtgt caataatggg aaatgaagtt
     4141 ttccgtttta atttggattt gtatccagat gctactgagg gggatttgta tactgacatg
     4201 tccaaagtgg atggtgtgct gtctctgaat gttggctgta ttcagattgt ctatcttcat
     4261 aaattcctta tgtcacttct gaacttcctg aataatttcc agacagccaa agagtctctg
     4321 agtgctgcca ctgcccaggc tgcagaaagg gctgccacaa gtgtgaaaga tcttgcccag
     4381 aggagttttc gtgtttccat caatattgat ttgaaagcac cggttatagt catcccacag
     4441 tcttctattt ccaccaatgc agtagtggta gatcttgggt taatcagagt tcataatcag
     4501 ttcagtctgg tgtctgatga agactactta aatcctccag taattgatag aatggatgtg
     4561 cagctaacaa agcttacact ttataggaca gtgatccagc caggcatcta ccatcctgat
     4621 attcagctgt tgcacccaat taacttggaa tttcttgtaa atcggaatct agctgcatct
     4681 tggtaccaca aggtgcctgt tgtggaaatt aaaggacatc ttgattcaat gaatgttagt
     4741 ctaaatcaag aagatcttaa tcttttattt aggatactaa cagaaaatct ctgtgagggt
     4801 actgaagact tggataaagt gaaaccaaga gtacaagaga caggtgaaat taaagagccc
     4861 cttgaaatct ctatatcaca agatgtacat gattcaaaaa atactttaac aactggagtg
     4921 gaagaaatta ggtctgtaga catcattaat atgctgctga attttgaaat taaagaggtt
     4981 gtggttactt tgatgaaaaa atcagaaaag aaaggaaggc ctttacatga gctaaatgtc
     5041 ctgcaacttg gaatggaagc taaagttaaa acctatgaca tgactgctaa agcttatcta
     5101 aaaaaaatta gtatgcagtg ctttgatttc actgactcta aaggggaacc tcttcacatt
     5161 attaactctt ctaatgtgac tgacgaaccc cttctgaaaa tgttactgac aaaggcagac
     5221 agtgatggac cagaatttaa aactattcat gacagtacca aacagagact gaaggtttca
     5281 tttgcatcct tagacttagt acttcatttg gaagctttac tttccttcat ggatttttta
     5341 tcatctgctg ctccattctc tgagccttcc tcttctgaga aggaatccga gctgaaacca
     5401 cttgtggggg agtccagaag tatcgctgtc aaagctgtat ccagcaacat ttcccaaaag
     5461 gatgtgtttg atttaaagat cacagctgaa ttaaatgcat ttaatgtctt tgtctgtgat
     5521 cagaagtgta acattgcaga tattaaaata catggaatgg atgcctctat ttctgtgaag
     5581 cctaagcaga ctgatgtgtt tgccagactt aaagatatta tagttatgaa tgtagatttg
     5641 cagtccattc acaaaaaggc tgtctctatt ttgggagatg aagtctttag gttccaactg
     5701 actctttatc cagatgccac agaaggagag gcctatgctg atatgtccaa agtagacggc
     5761 aaacttagtt ttaaagtggg ttgtattcag attgtttatg ttcataaatt cttcatgtct
     5821 cttttgaact tcctcaacaa tttccaaact gctaaagaag ctttgagtac agccacagtc
     5881 caggctgcag aaagagctgc ttccagcatg aaagacttgg ctcaaaagag tttccgcctt
     5941 ttgatggata ttaatttgaa agcaccagtt attattattc ctcagtcttc agtatcacct
     6001 aatgctgtta tagcagatct gggtttaatc agagttgaaa acaagtttag cttggttcct
     6061 atggaacatt attctcttcc tccagtcatt gataaaatga acatcgaact cactcagttg
     6121 aagctgtcaa gaactatttt gcaggctagc ttgccacaaa atgacattga aattttaaaa
     6181 ccagtcaaca tgcttttgtc catacagcga aacttagcag cagcatggta tgtgcaaatt
     6241 ccagggatgg agataaaagg aaaactaaaa cctatgcagg ttgctctcag tgaagatgac
     6301 ttgacagttt taatgaaaat tttgctagaa aatcttggag aagcttcctc acaaccaagc
     6361 cctacacagt ctgtgcagga gactgtaaga gtgagaaaag ttgatgtttc aagtgtacct
     6421 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     6481 gacgtaaacg gccacaagtt cagcgtccgc ggcgagggcg agggcgatgc caccaacggc
     6541 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     6601 gtgaccacct tcggctacgg cgtggcctgc ttcagccgct accccgacca catgaagcag
     6661 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catctctttc
     6721 aaggacgacg gtacctacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     6781 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     6841 ctggagtaca acttcaacag ccactacgtc tatatcacgg ccgacaagca gaagaactgc
     6901 atcaaggcta acttcaagat ccgccacaac gttgaggacg gcagcgtgca gctcgccgac
     6961 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     7021 ctgagccatc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     7081 ctggagttcg tgaccgccgc cggtggagac catctcaaag aacaagaaga ttggacagac
     7141 tcaaagctct ctatgaacca gattgtcagt ctccaatttg actttcactt tgaatctctt
     7201 tccattatcc tttataacaa tgatatcaac caggaatctg gagttgcatt tcataatgac
     7261 agtttccaac ttggtgaact cagactacat cttatggcct cctcagggaa gatgtttaag
     7321 gatggctcaa tgaatgtcag cgttaaactt aagacatgca cccttgatga tctcagagaa
     7381 ggaattgaga gagcaacatc gagaatgatt gacagaaaga atgaccaaga taacaacagt
     7441 tctatgattg atataagtta caaacaagac aaaaatggaa gtcaaattga tgctgttctt
     7501 gacaagctgt atgtatgtgc cagtgtggaa tttctgatga ctgtggcaga tttctttatc
     7561 aaagctgtgc ctcagagtcc agaaaatgtg gcaaaagaaa cacagatttt accaagacag
     7621 actgccacag ggaaggtcaa gatagagaaa gatgactctg ttagaccaaa tatgacttta
     7681 aaggccatga tcacagatcc agaagtggta tttgttgcca gcctgacaaa ggctgatgct
     7741 cctgctctga cagcctcgtt tcagtgcaac ctttctctgt caacatccaa actcgaacag
     7801 atgatggaag cttctgtgag agatctgaaa gtgctcgctt gcccttttct cagagaaaag
     7861 agagggaaaa acattaccac agtcttgcag ccctgttctt tatttatgga aaaatgtacg
     7921 tgggcttcag gaaagcaaaa tataaatatt atggttaaag aatttataat taagatttca
     7981 cccataattc ttaatactgt gttgacaatc atggctgcat tgtctccaaa aacaaaagaa
     8041 gatggatcca aagatacgtc taaggaaatg gaaaatcttt ggggtatcaa atcgattaat
     8101 gattataaca cttggtttct tggtgttgac acggcaacag aaataacgga aagcttcaaa
     8161 ggcattgaac attcactgat agaggaaaat tgtggtgttg ttgtagaatc cattcaagtt
     8221 accttagaat gtggccttgg acatcgaact gtacctttat tattggcaga gtctaagttt
     8281 tcaggaaata ttaaaaattg gacttctcta atggctgctg ttgctgacgt gacactacag
     8341 gtgcactatt acaatgagat ccatgctgtc tgggagccac tgattgagag agtggagggg
     8401 aagagacaat ggaatttaag gcttgatgta aagaagaacc cagttcagga taaaagtttg
     8461 ctgccaggag atgattttat tcctgagcca caaatggcaa ttcatatttc ttcaggaaat
     8521 acaatgaata taacaatatc caaaagttgt cttaatgttt tcaacaattt agcaaaaggt
     8581 ttttcagagg gcactgcttc tacttttgac tactctttaa aggacagagc tccttttacg
     8641 gtaaaaaatg ctgtaggtgt tcccattaag gtgaagccca attgtaatct cagagtaatg
     8701 ggcttccctg agaaaagtga tatttttgat gttgatgctg gccagaattt ggaactggag
     8761 tatgccagca tggtaccttc aagtcaaggg aacctatcta tattgagccg tcaagaaagc
     8821 tccttcttca ctctgaccat tgtacctcat ggatatacag aagttgcaaa tatccctgtg
     8881 gccagacctg gacggcgatt gtataatgta cggaatccca atgccagtca ttctgactct
     8941 gtcttggtac aaattgatgc aactgaaggg aataaagtaa ttacccttcg ctctcctcta
     9001 cagatcaaaa accatttctc cattgcattt atcatctata aatttgttaa gaatgttaag
     9061 ctattggagc gcattgggat agccagacct gaagaggagt tccatgttcc tttagattca
     9121 tatagatgtc aattgtttat ccagccagct ggaatcttag agcatcagta caaagaatct
     9181 accacttata tttcctggaa ggaagaactt cataggagca gggaagtcag atgcatgttg
     9241 cagtgtccat cagtagaagt cagcttctta cctctcatag tgaatacagt tgctctgcct
     9301 gatgaattga gctacatatg tacacatggg gaagactggg atgtagctta cattattcat
     9361 ctttatcctt ctctcacttt gcggaatctt ctcccatatt ccctaagata tttacttgag
     9421 ggaacagcag aaactcatga gctggcagaa ggcagtactg ctgatgttct gcattcgaga
     9481 atcagtggtg aaataatgga attagtcctg gtgaaatacc agggcaaaaa ctggaatgga
     9541 catttccgca tacgtgatac actaccagaa ttctttcctg tgtgtttttc ttctgactcc
     9601 acagaagtga cgacagtcga cctgtcagtc cacgtcagga gaattggcag ccggatggtg
     9661 ctgtctgtct ttagtcccta ttggttaatc aacaagacta cccgggttct ccagtatcgt
     9721 tcagaagata ttcatgtgaa acatccagct gatttcaggg atattatttt attttctttc
     9781 aagaagaaga acatttttac taaaaataag gtacaattaa aaatttcaac cagtgcctgg
     9841 tccagtagtt tctcattgga tacagtggga agttatgggt gtgtgaagtg tcctgccaac
     9901 aatatggagt acctggttgg tgttagcatc aaaatgagca gtttcaacct ttcacgaata
     9961 gttaccctga ctcccttttg taccattgca aacaagtcat cattagaact agaagttggc
    10021 gagattgcat ctgatggctc aatgccaact aataaatgga actatattgc ttcttcagag
    10081 tgccttccat tttggccaga aagtttgtca ggcaaacttt gtgtgagagt ggtgggctgt
    10141 gaaggatctt ccaaaccatt cttttataac cgacaggata atggcacttt attgagctta
    10201 gaagatctga atgggggtat cttggtggat gtaaacactg ccgaacattc aactgtcata
    10261 actttttctg attaccatga gggatctgca cctgccttga taatgaacca tacaccatgg
    10321 gacatcctca catacaaaca gagtgggtca ccagaagaaa tggtcttgct gccaagacag
    10381 gctcgacttt ttgcctgggc agatcctact ggtaccagaa aacttacatg gacatatgca
    10441 gcaaatgttg gggaacatga tctgttaaag gatggatgtg gacagtttcc atatgatgca
    10501 aacatccaga tacactgggt atcatttctg gatgggcgcc agagagtttt gcttttcacc
    10561 gatgatgttg ccttggtttc caaagcactg caggcagaag aaatggaaca ggctgattat
    10621 gaaataacct tgtctctcca cagtcttggg ctttcactgg ttaacaatga aagcaagcag
    10681 gaagtttcct atattgggat aaccagttct ggtgttgttt gggaggtgaa accaaagcag
    10741 aaatggaagc catttagtca aaagcagata atcttattgg aacaatccta tcagaaacat
    10801 caaatatcaa gagaccatgg ctggattaag ctagataata attttgaggt caattttgat
    10861 aaagatccaa tggaaatgcg cctccctatt cgtagcccta ttaaacgaga ctttttatca
    10921 ggaattcaga ttgaatttaa gcagtcttct caccagagaa gtttaagggc caggttgtac
    10981 tggcttcagg ttgataatca gttaccaggt gcaatgttcc ctgttgtatt tcatcctgtt
    11041 gcccctccaa aatctattgc tttagattca gagcccaagc ctttcattga tgtgagtgtc
    11101 atcacaagat ttaatgagta cagtaaagtc ttacagttca agtattttat ggtcctcatt
    11161 caggaaatgg ccttaaaaat tgatcaaggg tttctaggag ctattattgc actgtttacc
    11221 ccaacaacag accctgaagc tgaaagaaga cggacaaagt taatccaaca agatattgat
    11281 gctctaaatg cagaattaat ggagacttca atgactgata tgtcaattct tagtttcttt
    11341 gaacatttcc atatttctcc tgtgaagttg catttgagtt tgtctttggg ttccggaggt
    11401 gaagaatcag acaaagaaaa acaggaaatg tttgcagttc attctgtcaa cttgctgttg
    11461 aaaagcatag gtgctactct gactgatgtg gatgacctta tattcaaact tgcttattat
    11521 gaaattcgat atcagttcta caagagagat cagcttatat ggagtgttgt taggcattac
    11581 agtgaacagt tcttgaaaca gatgtatgtc cttgtattgg ggttagatgt acttggaaac
    11641 ccatttggat taattagagg tctgtctgaa ggagttgaag ctttattcta tgaacccttc
    11701 cagggtgctg ttcaaggccc tgaagaattt gcagaggggt tagtgattgg agtgagaagc
    11761 ctctttggac acacagtagg tggtgcagca ggagttgtat ctcgaatcac cggttctgtt
    11821 gggaaaggtt tggcagcaat tacaatggac aaggaatatc agcaaaaaag aagagaagag
    11881 ttgagtcgac agcccagaga ttttggagac agcctggcca gaggaggaaa gggctttctg
    11941 cgaggagttg ttggtggagt gactggaata ataacaaaac ctgtggaagg tgccaaaaag
    12001 gaaggagctg ctggattctt taaaggaatt ggaaaagggc ttgtgggtgc tgtggcccgt
    12061 ccaactggtg gaatcgtaga tatggccagt agtaccttcc aaggcattca gagggcagca
    12121 gaatcaactg aggaagtatc tagcctccgt ccccctcgcc tgatccatga agatggcatc
    12181 attcgtcctt atgacagaca ggaatctgag ggctctgact tacttgagaa tcatatcaaa
    12241 aagttggaag gagagactta ccgataccac tgtgctattc ctggaagcaa gaagacaatc
    12301 cttatggtta caaataggcg agtgttgtgt ataaaggaag ttgaaatcct gggccttatg
    12361 tgtgtagact ggcaatgtcc atttgaagat tttgtatttc ctcctagtgt cagtgaaaat
    12421 gtgctaaaaa tttcagttaa ggaacagggt ctgttccaca aaaaagacag tgccaatcaa
    12481 ggctgtgttc gaaaagttta cctgaaggac accgccacag cagagagagc atgtaatgcc
    12541 attgaggatg cacagtcaac gagacagcag caaaaattga tgaagcagtc atcagtgaga
    12601 cttctcagac cccaattgcc atcttaaaaa gcggccgcga ctctagatca taatcagcca
    12661 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
    12721 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
    12781 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    12841 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt
    12901 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
    12961 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
    13021 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
    13081 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
    13141 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
    13201 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
    13261 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
    13321 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
    13381 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
    13441 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg
    13501 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
    13561 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
    13621 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
    13681 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
    13741 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
    13801 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca
    13861 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
    13921 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
    13981 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
    14041 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
    14101 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
    14161 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
    14221 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
    14281 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
    14341 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
    14401 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
    14461 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
    14521 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
    14581 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
    14641 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
    14701 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
    14761 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
    14821 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
    14881 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
    14941 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
    15001 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
    15061 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact
    15121 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    15181 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    15241 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
    15301 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    15361 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
    15421 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    15481 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    15541 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    15601 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    15661 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    15721 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    15781 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    15841 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    15901 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat
    15961 gcat
//