| 质粒名称: | pFLP3 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 64946 |
| 存储实验室: | Herbert Schweizer |
|
载体骨架基本信息 |
|
| 载体名称: | pFLP1 |
| 载体出品公司: | Herbert Schweizer |
| 载体抗性: | 氨苄青霉素和四环素 |
| 空载体大小: | 10925 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Flp recombinase |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pFLP3 was a gift from Herbert Schweizer (Addgene plasmid # 64946 ; http://n2t.net/addgene:64946 ; RRID:Addgene_64946) |
| 参考文献部分: | A Tn7-based broad-range bacterial cloning and expression system. Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer RR, Schweizer HP. Nat Methods. 2005 Jun;2(6):443-8. 10.1038/nmeth765 PubMed 15908923 |
买家导航
pFLP3载体质粒基本信息
pFLP3质粒图谱载体图谱和pFLP3载体序列质粒序列多克隆位点信息
pFLP3质粒载体简介
pFLP3质粒序列载体序列
LOCUS pFLP3 10925 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Flp recombinase expression vector.
ACCESSION .
VERSION .
KEYWORDS pFLP3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10925)
AUTHORS Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer
RR, Schweizer HP
TITLE A Tn7-based broad-range bacterial cloning and expression system.
JOURNAL Nat Methods. 2005 Jun;2(6):443-8.
PUBMED 15908923
REFERENCE 2 (bases 1 to 10925)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10925
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(29..51)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 151..170
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
rep_origin 232..583
/label=pRO1600 oriV
/note="broad-host-range origin of replication from
Pseudomonas aeruginosa plasmid pRO1600; requires the
pRO1600 Rep protein for replication (West et al., 1994)"
CDS 597..1430
/codon_start=1
/product="replication protein for the broad-host-range
plasmid pRO1600 from Pseudomonas aeruginosa "
/label=pRO1600 Rep
/translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
primer_bind 1576..1598
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 1590..1607
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 1591..1607
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(2338..3759)
/codon_start=1
/gene="Bacillus subtilis sacB"
/product="secreted levansucrase that renders bacterial
growth sensitive to sucrose"
/label=SacB
/note="negative selection marker"
/translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
VKDSILEQGQLTVNK"
promoter complement(3760..4205)
/gene="sacR"
/label=sacB promoter
/note="sacB promoter and control region"
promoter 4420..4448
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
CDS 4496..5686
/codon_start=1
/gene="tet"
/product="tetracycline efflux protein"
/label=TcR
/note="confers resistance to tetracycline"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
primer_bind complement(4848..4867)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
CDS complement(6054..7325)
/codon_start=1
/product="site-specific recombinase"
/label=FLP
/note="FLP is a site-specific recombinase from
Saccharomyces cerevisiae. Recombination occurs at FRT
sequences."
/translation="MPQFGILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
YPAWNGIISQEVLDYLSSYINRRI"
RBS 7329..7337
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
CDS 7426..8139
/codon_start=1
/gene="cIts"
/product="temperature-sensitive variant of the phage lambda
repressor"
/label=-lambda repressor (ts)
/note="thermosensitivity is conferred by the A67T mutation"
/translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
PVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
VGKVIASQWPEETFG"
primer_bind complement(8428..8444)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8428..8444)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8441..8463)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8452..8468
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8476..8506)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8521..8542
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8659..8676)
/label=L4440
/note="L4440 vector, forward primer"
oriT 8926..9034
/note="incP origin of transfer"
rep_origin complement(9106..9694)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9186..9205)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9865..10725)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10488..10507
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10726..10830)
/gene="bla"
/label=AmpR promoter
primer_bind 10898..10916
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatacctc tcaggcgccg ctggtgccgc tggttggacg ccaagggtga atccgcctcg
241 ataccctgat tactcgcttc ctgcgccctc tcaggcggcg ataggggact ggtaaaacgg
301 ggattgccca gacgcctccc ccgccccttc aggggcacaa atgcggcccc aacggggcca
361 cgtagtggtg cgttttttgc gtttccaccc ttttcttcct tttccctttt aaacctttta
421 ggacgtctac aggccacgta atccgtggcc tgtagagttt aaaaagggac ggatttgttg
481 ccattaaggg acggatttgt tgttaagaag ggacggattt gttgttgtaa agggacggat
541 ttgttgtatt gtgggacgca gatacagtgt ccccttatac acaaggaatg tcgaacgtgg
601 cctcaccccc aatggtttac aaaagcaatg ccctggtcga ggccgcgtat cgcctcagtg
661 ttcaggaaca gcggatcgtt ctggcctgta ttagccaggt gaagaggagc gagcctgtca
721 ccgatgaagt gatgtattca gtgacggcgg aggacatagc gacgatggcg ggtgtcccta
781 tcgaatcttc ctacaaccag ctcaaagaag cggccctgcg cctgaaacgg cgggaagtcc
841 ggttaaccca agagcccaat ggcaagggga aaagaccgag tgtgatgatt accggctggg
901 tgcaaacaat catctaccgg gagggtgagg gccgtgtaga actcaggttc accaaagaca
961 tgctgccgta cctgacggaa ctcaccaaac agttcaccaa atacgccttg gctgacgtgg
1021 ccaagatgga cagcacccac gcgatcaggc tttacgagct gctcatgcaa tgggacagca
1081 tcggccagcg cgaaatagaa attgaccagc tgcgaaagtg gtttcaactg gaaggccggt
1141 atccctcgat caaggacttc aagttgcgag tgcttgatcc agccgtgacg cagatcaacg
1201 agcacagccc gctacaggtg gagtgggcgc agcgaaagac cgggcgcaag gtcacacatc
1261 tgttgttcag ttttggaccg aagaagcccg ccaaggcggt gggtaaggcc ccagcgaagc
1321 gcaaggccgg gaagatttca gatgctgaga tcgcgaaaca ggctcgccct ggtgagacat
1381 gggaagcggc ccgcgctcga ctaacccaga tgccgctgga tctggcctag aggccgtggc
1441 caccacggcc cggcctgcct ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
1501 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
1561 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat
1621 gcctgcagtt cacttacacc gcttctcaac ccggtacgca ccagaaaatc attgatatgg
1681 ccatgaatgg cgttggatgc cgggcaacag cccgcattat gggcgttggc ctcaacacga
1741 ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac agccgggcag
1801 tgacgtcatc gtctgcgcgg aaatggacga acagtggggc tatgtcgggg ctaaatcgcg
1861 ccagcgctgg ctgttttacg cgtatgacag tctccggaag acggttgttg cgcacgtatt
1921 cggtgaacgc actatggcga cgctggggcg tcttatgagc ctgctgtcac cctttgacgt
1981 ggtgatatgg atgacggatg gctggccgct gtatgaatcc cgcctgaagg gaaagctgca
2041 cgtaatcagc aagcgatata cgcagcgaat tgagcggcat aacctgaatc tgaggcagca
2101 cctggcacgg ctgggacgga agtcgctgtc gttctcaaaa tcggtggagc tgcatgacaa
2161 agtcatcggg cattatctga acataaaaca ctatcaataa gttggagtca ttaccaaaag
2221 gttaggaata cggttagcca tttgcctgct tttatatagt tcatatggga ttcaccttta
2281 tgttgataag aaataaaaga aaatgccaat aggatatcgg cattttcttt tgcgttttta
2341 tttgttaact gttaattgtc cttgttcaag gatgctgtct ttgacaacag atgttttctt
2401 gcctttgatg ttcagcagga agctaggcgc aaacgttgat tgtttgtctg cgtagaatcc
2461 tctgtttgtc atatagcttg taatcacgac attgtttcct ttcgcttgag gtacagcgaa
2521 gtgtgagtaa gtaaaggtta catcgttagg atcaagatcc atttttaaca caaggccagt
2581 tttgttcagc ggcttgtatg ggccagttaa agaattagaa acataaccaa gcatgtaaat
2641 atcgttagac gtaatgccgt caatcgtcat ttttgatccg cgggagtcag tgaacagata
2701 ccatttgccg ttcattttaa agacgttcgc gcgttcaatt tcatctgtta ctgtgttaga
2761 tgcaatcagc ggtttcatca cttttttcag tgtgtaatca tcgtttagct caatcatacc
2821 gagagcgccg tttgctaact cagccgtgcg ttttttatcg ctttgcagaa gtttttgact
2881 ttcttgacgg aagaatgatg tgcttttgcc atagtatgct ttgttaaata aagattcttc
2941 gccttggtag ccatcttcag ttccagtgtt tgcttcaaat actaagtatt tgtggccttt
3001 atcttctacg tagtgaggat ctctcagcgt atggttgtcg cctgagctgt agttgccttc
3061 atcgatgaac tgctgtacat tttgatacgt ttttccgtca ccgtcaaaga ttgatttata
3121 atcctctaca ccgttgatgt tcaaagagct gtctgatgct gatacgttaa cttgtgcagt
3181 tgtcagtgtt tgtttgccgt aatgtttacc ggagaaatca gtgtagaata aacggatttt
3241 tccgtcagat gtaaatgtgg ctgaacctga ccattcttgt gtttggtctt ttaggataga
3301 atcatttgca tcgaatttgt cgctgtcttt aaagacgcgg ccagcgtttt tccagctgtc
3361 aatagaagtt tcgccgactt tttgatagaa catgtaaatc gatgtgtcat ccgcattttt
3421 aggatctccg gctaatgcaa agacgatgtg gtagccgtga tagtttgcga cagtgccgtc
3481 agcgttttgt aatggccagc tgtcccaaac gtccaggcct tttgcagaag agatattttt
3541 aattgtggac gaatcgaact caggaacttg atatttttca tttttttgct gttcagggat
3601 ttgcagcata tcatggcgtg taatatggga aatgccgtat gtttccttat atggcttttg
3661 gttcgtttct ttcgcaaacg cttgagttgc gcctcctgcc agcagtgcgg tagtaaaggt
3721 taatactgtt gcttgttttg caaacttttt gatgttcatc gttcatgtct ccttttttat
3781 gtactgtgtt agcggtctgc ttcttccagc cctcctgttt gaagatggca agttagttac
3841 gcacaataaa aaaagaccta aaatatgtaa ggggtgacgc caaagtatac actttgccct
3901 ttacacattt taggtcttgc ctgctttatc agtaacaaac ccgcgcgatt tacttttcga
3961 cctcattcta ttagactctc gtttggattg caactggtct attttcctct tttgtttgat
4021 agaaaatcat aaaaggattt gcagactacg ggcctaaaga actaaaaaat ctatctgttt
4081 cttttcattc tctgtatttt ttatagtttc tgttgcatgg gcataaagtt gcctttttaa
4141 tcacaattca gaaaatatca taatatctca tttcactaaa taatagtgaa cggcaggtat
4201 atgtgatggg ttaaaaagga tcgatcctct agagaatagg aacttcggaa taggaacttc
4261 aagatcccct gattcccttt gtcaacagca atggatactg actcgctgcg ctcggtcgtt
4321 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
4381 ggggataacg caggaaagaa catgaattaa ttctcatgtt tgacagctta tcatcgatta
4441 gctttaatgc ggtagtttat cacagttaaa ttgctaacgc agtcaggcac cgcgtatgaa
4501 atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg
4561 cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg acagcatcgc
4621 cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat gcgcacccgt
4681 tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt cgctacttgg
4741 agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggattc tctacgccgg
4801 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
4861 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
4921 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tacatgcacc
4981 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
5041 ggagtcgcat aagggagagc gccgaccgat gcccttgaga gccttcaacc cagtcagctc
5101 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat
5161 gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg
5221 ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc
5281 tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc
5341 cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat
5401 ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc
5461 catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc
5521 tcttaccagc ctaacttcga tcactggacc gctgatcgtc acggcgattt atgccgcctc
5581 ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct
5641 ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg aagccggcgg
5701 cacctcgcta acggattcac cactccaaga attggagcca atcaattctt gcggagaact
5761 gtgaatgcgc aaaccaaccc ttggggctgc aggaattcca ttgagtaagt ttttaagcac
5821 atcagcttca aaagcgctct gaagttccta tactttctag aggatccccc tagagaatag
5881 gaacttcgga ataggaactt caaagcgttt ccgaaaacga gcgcttccga aaatgcaacg
5941 cgagctgcgc acatacagct cactgttcac gtcgcaccta tatctgcgtg ttgcctgtat
6001 atatatatac atgagaagaa cggcatagtg cgtgtttatg cttaaatgcg tacttatatg
6061 cgtctattta tgtaggatga aaggtagtct agtacctcct gtgatattat cccattccat
6121 gcggggtatc gtatgcttcc ttcagcacta ccctttagct gttctatatg ctgccactcc
6181 tcaattggat tagtctcatc cttcaatgct atcatttcct ttgatattgg atcatatgca
6241 tagtaccgag aaactagtgc gaagtagtga tcaggtattg ctgttatctg atgagtatac
6301 gttgtcctgg ccacggcaga agcacgctta tcgctccaat ttcccacaac attagtcaac
6361 tccgttaggc ccttcattga aagaaatgag gtcatcaaat gtcttccaat gtgagatttt
6421 gggccatttt ttatagcaaa gattgaataa ggcgcatttt tcttcaaagc tttattgtac
6481 gatctgacta agttatcttt taataattgg tattcctgtt tattgcttga agaattgccg
6541 gtcctattta ctcgttttag gactggttca gaattcctca aaaattcatc caaatataca
6601 agtggatcga tcctacccct tgcgctaaag aagtatatgt gcctactaac gcttgtcttt
6661 gtctctgtca ctaaacactg gattattact cccagatact tattttggac taatttaaat
6721 gatttcggat caacgttctt aatatcgctg aatcttccac aattgatgaa agtagctagg
6781 aagaggaatt ggtataaagt ttttgttttt gtaaatctcg aagtatactc aaacgaattt
6841 agtattttct cagtgatctc ccagatgctt tcaccctcac ttagaagtgc tttaagcatt
6901 tttttactgt ggctatttcc cttatctgct tcttccgatg attcgaactg taattgcaaa
6961 ctacttacaa tatcagtgat atcagattga tgtttttgtc catagtaagg aataattgta
7021 aattcccaag caggaatcaa tttctttaat gaggcttcca gaattgttgc tttttgcgtc
7081 ttgtatttaa actggagtga tttattgaca atatcgaaac tcagcgaatt gcttatgata
7141 gtattatagc tcatgaatgt ggctctcttg attgctgttc cgttatgtgt aatcatccaa
7201 cataaatagg ttagttcagc agcacataat gctattttct cacctgaagg tctttcaaac
7261 ctttccacaa actgacgaac aagcacctta ggtggtgttt tacataatat accaaattgt
7321 ggcatacaac ctccttagta catgcaacca ttatcaccgc cagaggtaaa atagtcaaca
7381 cgcacggtgt tagatattta tcccttgcgg tgatagattt aacgtatgag cacaaaaaag
7441 aaaccattaa cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat ttatgaaaaa
7501 aagaaaaatg aacttggctt atcccaggaa tctgtcgcag acaagatggg gatggggcag
7561 tcaggcgttg gtgctttatt taatggcatc aatgcattaa atgcttataa cgccgcattg
7621 cttacaaaaa ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc cagagaaatc
7681 tacgagatgt atgaagcggt tagtatgcag ccgtcactta gaagtgagta tgagtaccct
7741 gttttttctc atgttcaggc agggatgttc tcacctaagc ttagaacctt taccaaaggt
7801 gatgcggaga gatgggtaag cacaaccaaa aaagccagtg attctgcatt ctggcttgag
7861 gttgaaggta attccatgac cgcaccaaca ggctccaagc caagctttcc tgacggaatg
7921 ttaattctcg ttgaccctga gcaggctgtt gagccaggtg atttctgcat agccagactt
7981 gggggtgatg agtttacctt caagaaactg atcagggata gcggtcaggt gtttttacaa
8041 ccactaaacc cacagtaccc aatgatccca tgcaatgaga gttgttccgt tgtggggaaa
8101 gttatcgcta gtcagtggcc tgaagagacg tttggctgat cggcaaggtg ttctggtcgg
8161 cgcatagctg ataacaattg agcaagaatc ttcatcgaat taggggaatt ttcactcccc
8221 tcagaacata acatagtaaa tggattgaat tatgaagaat ggtttttatg cgacttaccg
8281 cagcaaaaat aaagggaaag ataagcgctc aataaacctg tctgttttcc ttaattctct
8341 gctggctgat aatcatcacc tgcaggttgg ctccaattat ttgtatattc ataaaatcgg
8401 ggtaccgagc tcgaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
8461 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
8521 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
8581 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
8641 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
8701 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
8761 aggaaagaac atggggattc cttaaggtat actttccgct gcataaccct gcttcggggt
8821 cattatagcg attttttcgg tatatccatc ctttttcgca cgatatacag gattttgcca
8881 aagggttcgt gtagactttc cttggtgtat ccaacggcgt cagccgggca ggataggtga
8941 agtaggccca cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc acctggcggt
9001 gctcaacggg aatcctgctc tgcgaggctg gccgataagc tagcttatgt gagcaaaagg
9061 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
9121 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
9181 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
9241 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
9301 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
9361 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
9421 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
9481 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
9541 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
9601 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
9661 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
9721 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
9781 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
9841 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
9901 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
9961 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
10021 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
10081 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
10141 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
10201 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
10261 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
10321 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
10381 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
10441 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
10501 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
10561 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
10621 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
10681 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
10741 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
10801 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
10861 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
10921 tcgtc
//
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