pEff

LOCUS       pEff               14502 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Transient expression of recombinant proteins in plants. The plasmid 
            contains gfp, which is replaced by the gene of interest..
ACCESSION   .
VERSION     .
KEYWORDS    pEff
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14502)
  AUTHORS   Mardanova ES, Blokhina EA, Tsybalova LM, Peyret H, Lomonossoff GP, 
            Ravin NV
  TITLE     Efficient Transient Expression of Recombinant Proteins in Plants by 
            the Novel pEff Vector Based on the Genome of Potato Virus X.
  JOURNAL   Front Plant Sci. 2017 Feb 28;8:247. doi: 10.3389/fpls.2017.00247. 
            eCollection 2017.
  PUBMED    28293244
REFERENCE   2  (bases 1 to 14502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..14502
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      1052..1061
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     complement(4925..4946)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5186..5205)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5607..5628
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      5872..6124
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        6213..6558
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      7250..7502
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        7658..7837
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        7690..7873
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             7894..8688
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7948..7967)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8558..8577
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      9074..9326
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    9417..9441
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      9809..10439
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(10529..11677)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11976..12770)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     complement(13360..13377)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(13611..13630)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    14062..14086
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     14225..14244
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14369..14391
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14383..14400
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14384..14400
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggagaaaac taaaccatac
      361 accaccaaca caaccaaacc caccacgccc aattgttaca cacccgcttg aaaaagaaag
      421 tttaacaaat ggccaaggtg cgcgaggttt accaatcttt tacagactcc accacaaaaa
      481 ctctcatcca agatgaggct tatagaaaca ttcgccccat catggaaaaa cacaaactag
      541 ctaaccctta cgctcaaacg gttgaagcgg ctaatgatct agaggggttc ggcatagcca
      601 ccaatcccta tagcattgaa ttgcatacac atgcagccgc taagaccata gagaataaac
      661 ttctagaggt gcttggttcc atcctaccac aagaacctgt tacatttatg tttcttaaac
      721 ccagaaagct aaactacatg agaagaaacc cgcggatcaa ggacattttc caaaatgttg
      781 ccattgaacc aagagacgta gccaggtacc ccaaggaaac aataattgac aaactcacag
      841 agatcacaac ggaaacagca tacattagtg acactctgca cttcttggat ccgagctaca
      901 tagtggagac attccaaaac tgcccaaaat tgcaaacatt gtatgcgacc ttagttctcc
      961 ccgttgaggc agcctttaaa atggaaagca ctcacccgaa catatacagc ctcaaatact
     1021 tcggagatgg tttccagtat ataccaggca accatggtgg cggggcatac catcatgaat
     1081 tcgctcatct acaatggctc aaagtgggaa agatcaagtg gagggacccc aaggatagct
     1141 ttctcggaca tctcaattac acgactgagc aggttgagat gcacacagtg acagtacagt
     1201 tgcaggaatc gttcgcggca aaccacttgt actgcatcag gagaggagac ttgctcacac
     1261 cggaggtgcg cactttcggc caacctgaca ggtacgtgat tccaccacag atcttcctcc
     1321 caaaagttca caactgcaag aagccgattc tcaagaaaac tatgatgcag ctcttcttgt
     1381 atgttaggac agtcaaggtc gcaaaaaatt gtgacatttt tgccaaagtc agacaattaa
     1441 ttaaatcatc tgacttggac aaatactctg ctgtggaact ggtttactta gtaagctaca
     1501 tggagttcct tgccgattta caagctacca cctgcttctc agacacactt tctggtggct
     1561 tgctaacaaa gacccttgca ccggtgaggg cttggataca agagaaaaag atgcagctgt
     1621 ttggtcttga ggactacgcg aagttagtca aagcagttga tttccacccg gtggattttt
     1681 ctttcaaagt ggaaacttgg gacttcagat tccacccctt gcaagcgtgg aaagccttcc
     1741 gaccaaggga agtgtcggat gtagaggaaa tggaaagttt gttctcagat ggggacttgc
     1801 ttgattgctt cacaagaatg ccagcttatg cggtaaacgc agaggaagat ttagctgcaa
     1861 tcaggaaaac gcccgagatg gatgtcggtc aagaagttaa agaacctgca ggagacagaa
     1921 atcaatactc aaaccctgca gaaactttcc tcaacaagct ccacaggaaa cacagtaggg
     1981 aggtgaaaca ccaggccgca aagaaagcta aacgcctagc tgaaatccag gagtcaatga
     2041 gagctgaagg tgatgccgaa ccaaatgaaa taagcggggg catgggggca atacccagca
     2101 acgccgaact tcctggcacg aatgatgcca gacaagaact cacactccca accactaaac
     2161 ctgtccctgc aaggtgggaa gatgcttcat tcacagattc tagtgtggaa gaggagcagg
     2221 ttaaacccct tggaaaagaa accgttgaaa cagcgacgca acaagtcatc gaaggacttc
     2281 cttggaaaca ctggattcct caattaaatg ctgttggatt caaggcgctg gaaattcaga
     2341 gggataggag tggaacaatg atcatgccca tcacagaaat ggtctccggg ctggaaaaag
     2401 aggacttccc tgaaggaact ccaaaagagt tggcacgaga attgttcgct atgaacagaa
     2461 gccctgccac catccctttg gacctgctta gagccagaga ctacggcagt gatgtaaaga
     2521 acaagagaat tggtgccatc acaaagacac aggcaacgag ttggggcgaa tacttgacag
     2581 gaaagataga aagcttgact gagaggaaag ttgcgacttg tgtcattcat ggagctggag
     2641 gttctggaaa aagtcatgcc atccagaagg cattgagaga aattggcaag ggctcggaca
     2701 tcactgtagt cctgccgacc aatgaactgc ggctagattg gagtaagaaa gtgcctaaca
     2761 ctgagcccta tatgttcaag acctatgaaa aggcgttaat tgggggaaca ggcagcatag
     2821 tcatctttga cgattactca aaacttcctc ccggttacat agaagcctta gtctgtttct
     2881 actctaaaat caagctaatc attctaacag gagatagcag acaaagcgtc taccatgaaa
     2941 ctgctgagga cgcctccatc aggcatttgg gaccagcaac agagtacttc tcaaaatact
     3001 gccgatacta tctcaatgcc acacaccgca acaagaaaga tcttgcgaac atgcttggtg
     3061 tctacagtga gagaacggga gtcaccgaaa tcagcatgag cgccgagttc ttagaaggaa
     3121 tcccaacttt ggtaccctcg gatgagaaga gaaagctgta catgggcacc gggaggaatg
     3181 acacgttcac atacgctgga tgccaggggc taactaagcc gaaggtacaa atagtgttgg
     3241 accacaacac ccaagtgtgt agcgcgaatg tgatgtacac ggcactttct agagccaccg
     3301 ataggattca cttcgtgaac acaagtgcaa attcctcggc cttctgggaa aagttggaca
     3361 gcacccctta cctcaagact ttcctatcag tggtgagaga acaagcactc agggagtacg
     3421 agccggcaga ggcagagcca attcaagagc ctgagcccca gacacacatg tgtgtcgaga
     3481 atgaggagtc cgtgctagaa gagtacaaag aggaactctt ggaaaagttt gacagagaga
     3541 tccactctga atcccatggt cattcaaact gtgtccaaac tgaagacaca accattcagt
     3601 tgttttcgca tcaacaagca aaagatgaga ccctcctctg ggcgactata gatgcgcggc
     3661 tcaagaccag caatcaagag acaaacttcc gagaattcct gagcaagaag gacattgggg
     3721 acgttctgtt tttaaactac caaaaagcta tgggtttacc caaagagcgt attccttttt
     3781 cccaagaggt ctgggaagct tgtgcccacg aagtacaaag caagtacctc agcaagtcaa
     3841 agtgcaactt gatcaatggg actgtgagac agagcccaga cttcgatgaa aataagatta
     3901 tggtattcct caagtcgcag tgggtcacaa aggtggaaaa actaggtcta cccaagatta
     3961 agccaggtca aaccatagca gccttttacc agcagactgt gatgcttttt ggaactatgg
     4021 ctaggtacat gcgatggttc agacaggctt tccagccaaa agaagtcttc ataaactgtg
     4081 agaccacgcc agatgacatg tctgcatggg ccttgaacaa ctggaatttc agcagaccta
     4141 gcttggctaa tgactacaca gctttcgacc agtctcagga tggagccatg ttgcaatttg
     4201 aggtgctcaa agccaaacac cactgcatac cagaggaaat cattcaggca tacatagata
     4261 ttaagactaa tgcacagatt ttcctaggca cgttatcaat tatgcgcctg actggtgaag
     4321 gtcccacttt tgatgcaaac actgagtgca acatagctta cacccataca aagtttgaca
     4381 tcccagccgg aactgctcaa gtttatgcag gagacgactc cgcactggac tgtgttccag
     4441 aagtgaagca tagtttccac aggcttgagg acaaattact cctaaagtca aagcctgtaa
     4501 tcacgcagca aaagaagggc agttggcctg agttttgtgg ttggctgatc acaccaaaag
     4561 gggtgatgaa agacccaatt aagctccatg ttagcttaaa attggctgaa gctaagggtg
     4621 aactcaagaa atgtcaagat tcctatgaaa ttgatctgag ttatgcctat gaccacaagg
     4681 actctctgca tgacttgttc gatgagaaac agtgtcaggc acacacactc acttgcagaa
     4741 cactaatcaa gtcagggaga ggcactgtct cactttcccg cctcagaaac tttctttaac
     4801 cgttaagtta ccttagagat ttgaataagt ctagattttt atttttaatt ttctttcaaa
     4861 tacttccatc aggcgcgcca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     4921 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     4981 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     5041 gcccgtgccc tggcccaccc tcgtgaccac cttcagctac ggcgtgcagt gcttcagccg
     5101 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     5161 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtatg
     5221 tagtgttttt ggataactga gtttgcctat gattttgtat ttactgagat gtttgtcctc
     5281 tttctgcagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     5341 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     5401 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     5461 cacaacaccg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     5521 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     5581 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     5641 atcactcacg gcatggacga gctgtacaag taacccgggt gtacaagtaa tctaggatac
     5701 cgtcgaggtc gtatcactgg aacaacaacc gctgaggctg ttgtcactct accaccacca
     5761 taactacgtc tacataaccg acgcctaccc cagtttcata gtattttctg gtttgattgt
     5821 atgaataata taaataaaaa aaaaaaacta gtgggtaccg cgaatttccc cgatcgttca
     5881 aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc
     5941 atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta
     6001 tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa
     6061 aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta
     6121 gatcgtatac ctagggtcaa catggtggag cacgacacac ttgtctactc caaaaatatc
     6181 aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc
     6241 ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa
     6301 aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat
     6361 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
     6421 gaagacggtc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta
     6481 agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca
     6541 tttcatttgg agagaacacg ggggactcta gaggatccga aatgcgggtg atagtatcac
     6601 catacgaagc tgaagacatt ttaaaaagat caacagacat gctacgaaac attgatagcg
     6661 gtgttttgtc tacaaaggaa tgcattaagg ctttcagcac catcactaga gacctgcact
     6721 gtgcaaaggc atcttaccaa tggggagttg acacaggttt gtatcaacgt aattgcgcag
     6781 aaaagcacct tattgataca gttgaaagta acataaggtt ggcacagccg cttgttaggg
     6841 agaaggtagc agtgcatttt tgcaaggatg agcctaaaga acttgttgcc ttcataacta
     6901 gaaaatatgt cgaattgact ggagtaggtg ttagggaagc tgttaaaagg gagatgcgga
     6961 gtctcactaa gacagttctc aataagatgt ccctcgaaat ggcattctat atgtcaccta
     7021 gagcttggaa gaacgcagaa tggctggaat tgaaatttag tcccgtaaag attttcagag
     7081 atttgcttct cgatgttgaa actctcaatg aactctgcgc agaggatgac gttcatgtcg
     7141 ataaagttaa tgaaaacggt gatgagaacc atgatctcga acttcaggat gagtgctgaa
     7201 tttaaattgt acaagtaaag cggccctgca ggtcgactcg aatttccccg atcgttcaaa
     7261 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     7321 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     7381 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     7441 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     7501 tctacgtacc ccgcgccagc ttggcgtaat catggtcata gctgttgcga tcgctctgca
     7561 gataacgcgt ggccggccat gagcggagaa ttaagggagt cacgttatga cccccgccga
     7621 tgacgcggga caagccgttt tacgtttgga actgacagaa ccgcaacgtt gaaggagcca
     7681 ctcagccgcg ggtttctgga gtttaatgag ctaagcacat acgtcagaaa ccattattgc
     7741 gcgttcaaaa gtcgcctaag gtcactatca gctagcaaat atttcttgtc aaaaatgctc
     7801 cactgacgtt ccataaattc ccctcggtat ccaattagag tctcatattc actctcaatc
     7861 caaataatct gcaccggatc tggatcgttt cgcatgattg aacaagatgg attgcacgca
     7921 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     7981 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     8041 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     8101 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     8161 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     8221 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     8281 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     8341 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     8401 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgatg atctcgtcgt gacccatggc
     8461 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     8521 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     8581 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     8641 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     8701 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     8761 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     8821 tccagcgcgg ggatctcatg ctggagttct tcgcccacgg gatctctgcg gaacaggcgg
     8881 tcgaaggtgc cgatatcatt acgacagcaa cggccgacaa gcacaacgcc acgatcctga
     8941 gcgacaatat gatcgcggcg tccacatcaa cggcgtcggc ggcgactgcc caggcaagac
     9001 cgagatgcac cgcgatatct tgctgcgttc ggatattttc gtggagttcc cgccacagac
     9061 ccggatgatc cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
     9121 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
     9181 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
     9241 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
     9301 cgcggtgtca tctatgttac tagatcgggg gccggccctc actggtgaaa agaaaaacca
     9361 ccccagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt
     9421 acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca
     9481 aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt
     9541 gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg
     9601 ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga
     9661 gcgttgctgc ctgtgatcaa atatcatctc cctcgcagag atccgaatta tcagccttct
     9721 tattcatttc tcgcttaacc gtgacagagt agacaggctg tctcgcggcc gaggggcgca
     9781 gcccctgggg ggatgggagg cccgcgttag cgggccggga gggttcgaga agggggggca
     9841 ccccccttcg gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa aacaggttta
     9901 taaatattgg tttaaaagca ggttaaaaga caggttagcg gtggccgaaa aacgggcgga
     9961 aacccttgca aatgctggat tttctgcctg tggacagccc ctcaaatgtc aataggtgcg
    10021 cccctcatct gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct catctgtcag
    10081 tagtcgcgcc cctcaagtgt caataccgca gggcacttat ccccaggctt gtccacatca
    10141 tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat ttgcgaggct ggccagctcc
    10201 acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa cgccgcgccg ggtgagtcgg
    10261 cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag ggccaagttt tccgcgaggt
    10321 atccacaacg ccggcggccg cggtgtctcg cacacggctt cgacggcgtt tctggcgcgt
    10381 ttgcagggcc atagacggcc gccagcccag cggcgagggc aaccagcccg gtgagcgtcg
    10441 gaaaggcgct cggtcttgcc ttgctcgtcg gtgatgtaca ctagtcgctg gctgctgaac
    10501 ccccagccgg aactgacccc acaaggccct agcgtttgca atgcaccagg tcatcattga
    10561 cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc gcaggcttcg ccgacctgct
    10621 cgcgccactt cttcacgcgg gtggaatccg atccgcacat gaggcggaag gtttccagct
    10681 tgagcgggta cggctcccgg tgcgagctga aatagtcgaa catccgtcgg gccgtcggcg
    10741 acagcttgcg gtacttctcc catatgaatt tcgtgtagtg gtcgccagca aacagcacga
    10801 cgatttcctc gtcgatcagg acctggcaac gggacgtttt cttgccacgg tccaggacgc
    10861 ggaagcggtg cagcagcgac accgattcca ggtgcccaac gcggtcggac gtgaagccca
    10921 tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt cgtgtaatac cggccattga
    10981 tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa ggtgatcggc tcgccgatag
    11041 gggtgcgctt cgcgtactcc aacacctgct gccacaccag ttcgtcatcg tcggcccgca
    11101 gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac gtggaaaatg accttgtttt
    11161 gcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa cagggcagag cgggccgtgt
    11221 cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat atcgaacaag gaaagctgca
    11281 tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc ctgcttggcc tcgctgacct
    11341 gttttgccag gtcctcgccg gcggtttttc gcttcttggt cgtcatagtt cctcgcgtgt
    11401 cgatggtcat cgacttcgcc aaacctgccg cctcctgttc gagacgacgc gaacgctcca
    11461 cggcggccga tggcgcgggc agggcagggg gagccagttg cacgctgtcg cgctcgatct
    11521 tggccgtagc ttgctggacc atcgagccga cggactggaa ggtttcgcgg ggcgcacgca
    11581 tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga aaaccccgcg tcgatcagtt
    11641 cttgcctgta tgccttccgg tcaaacgtcc gattcattca ccctccttgc gggattgccc
    11701 cgactcacgc cggggcaatg tgcccttatt cctgatttga cccgcctggt gccttggtgt
    11761 ccagataatc caccttatcg gcaatgaagt cggtcccgta gaccgtctgg ccgtccttct
    11821 cgtacttggt attccgaatc ttgccctgca cgaataccag cgaccccttg cccaaatact
    11881 tgccgtgggc ctcggcctga gagccaaaac acttgatgcg gaagaagtcg gtgcgctcct
    11941 gcttgtcgcc ggcatcgttg cgccacatct aggtactaaa acaattcatc cagtaaaata
    12001 taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca
    12061 tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac
    12121 ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca
    12181 aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc
    12241 gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt
    12301 tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg
    12361 tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac
    12421 tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa
    12481 aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc
    12541 aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc
    12601 acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt
    12661 tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg
    12721 tatttttcga tcagtttttt caattccggt gatattctca ttttagccat ttattatttc
    12781 cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact
    12841 ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt
    12901 tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa ccacaattat
    12961 gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg
    13021 cttctgtttc tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca
    13081 aaagcaccgc cggacatcag cgctatctct gctctcactg ccgtaaaaca tggcaactgc
    13141 agttcactta caccgcttct caacccggta cgcaccagaa aatcattgat atggccatga
    13201 atggcgttgg atgccgggca acagcccgca ttatgggcgt tggcctcaac acgattttac
    13261 gtcacttaaa aaactcaggc cgcagtcggt aactatgcgg tgtgaaatac cgcacagatg
    13321 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
    13381 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    13441 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    13501 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    13561 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    13621 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    13681 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    13741 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    13801 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    13861 cgacttatcg ccactggcag caggtaacct cgcgcataca gccgggcagt gacgtcatcg
    13921 tctgcgcgga aatggacggg cccccggcgc cagatctggg gaaccctgtg gttggcatgc
    13981 acatacaaat ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc gattattcta
    14041 ataaacgctc ttttctctta ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt
    14101 gtgaaccatc acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
    14161 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
    14221 aggaagggaa gaaagcgaaa ggagcgggcg ccattcaggc tgcgcaactg ttgggaaggg
    14281 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
    14341 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
    14401 gaattgttaa ttaataggcc tcgaggtcaa catggtggag cacgacacac ttgtctactc
    14461 caaaaatatc aaagatacag tctcagaaga ccaaagggca at
//