iSeroSnFR

LOCUS       iSeroSnFR          5842 bp DNA     circular SYN 12-MAY-2026
DEFINITION  PM20.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Kiryl Piatkevich
  TITLE     Direct Submission
  JOURNAL   Exported Jul 24, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     39..62
                     /label=N1-CMV-Fw
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    591..666
                     /label=MCS
                     /note="multiple cloning site of fluorescent protein 
                     plasmids"
     misc_feature    672..677
                     /label=Kozak
     misc_feature    678..2504
                     /label=iSeroSnFR
     CDS             678..740
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTGD"
     misc_feature    747..971
                     /label=X513 opuBC
     misc_feature    972..983
                     /label=LINKER 1
     primer_bind     1190..1211
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1707..1718
                     /label=LINKER 2
     misc_feature    1719..2309
                     /label=X513 opuBC
     CDS             2322..2351
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2355..2501
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     polyA_signal    2630..2751
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     2730..2752
                     /label=N1-CMV-Fw
     primer_bind     complement(2734..2764)
                     /label=N1-CMV-Rv
     primer_bind     complement(2749..2769)
                     /label=N1-CMV-Rv
     rep_origin      complement(2758..3213)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3240..3344
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3346..3703
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3554..3689
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3738..4532
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    4764..4811
                     /label=HSV TK poly(A) signal
                     /note="herpesvirus thymidine kinase polyadenylation signal"
     rep_origin      5140..5728
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      661 gatccaccgg tgccaccatg gagacagaca cactcctgct atgggtactg ctgctctggg
      721 ttccaggttc cactggtgac agatctgcga acgacaccgt agttgtgggc tcgattaacc
      781 atacagaaca gattatcgtc gcaaacatgt tggcagagat gattgaggcg catacagacc
      841 ttaaggtggt tcgcaaactg aaccttggcg gggttaacgt taactttgaa gccattaaac
      901 gcggaggtgc gaataatggt attgacattt acctggagta cgttgggtat ggtcttgtgg
      961 atattctggg gtacccggag ccgaacgtct atatcatcgc cgacaagcag aagaacggca
     1021 tcaaggcgaa cttcaagatc cgctacaacg tggaggacgg cagcgtgcag ctcgccgacc
     1081 actaccagca gaacaccccc atcggcgacg gccctgtgct gctgcctgac aaccactacc
     1141 tgagcaccca gtccgtgctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     1201 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggcggta
     1261 ccggagggag catgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     1321 agctggacgg cggcgtaaac ggccacaagt tcagcgtgcg cggcgagggc gagggcgatg
     1381 ccaccaacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     1441 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     1501 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1561 ccatcagctt caaggacgac ggcacctaca agacccgcgc cgaggtgaag ttcgagggcg
     1621 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     1681 tggggcacaa gctggagtac aactttccgc cgcccggtgc tactgatcca gaaggtgcat
     1741 acgaaaccgt gaagaaggag tacaaacgta aatggaatat tgtatggctc aaaccactgg
     1801 gattcaacgc ttcgtatgtg cttgcggtta aagacgaact ggcgaaacag tataacctta
     1861 aaaccttcag tgacttagcg aaaatctcgg ataagctgat tctgggtgca aacatgatgt
     1921 ttttagaaaa tcccgatggt tacccaggcc tgcaaaaact gtacaatttc aaattcaagc
     1981 acaccaaaag catggacgct ggtattccgt ataccgccat tgataataac gaagttcagg
     2041 taattgatgc cactgccact gatggcttgc tggtgagcca caaattaaaa attctggagg
     2101 atgataaagc gttcttcccg ccgtattatg ctgcccccat catccgtcag gatgtcttag
     2161 ataagcatcc tgaactgaag gacgtgctga acaaactcgc gaatcaaatt tcactggaag
     2221 aaatgcagaa actgaattac aagcgtgacg gtgagggtca ggacccagcg aaagtagcta
     2281 aggagttttt gaaagagaaa ggtttaattc tgcaggtcga cgaacaaaaa ctcatctcag
     2341 aagaggatct gaatgctgtg ggccaggaca cgcaggaggt catcgtggtg ccacactcct
     2401 tgccctttaa ggtggtggtg atctcagcca tcctggccct ggtggtgctc accatcatct
     2461 cccttatcat cctcatcatg ctttggcaga agaagccacg ttagtaaagc ggccgcgact
     2521 ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     2581 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     2641 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     2701 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa
     2761 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     2821 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     2881 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     2941 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     3001 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     3061 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     3121 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     3181 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
     3241 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3301 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa
     3361 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     3421 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     3481 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     3541 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     3601 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     3661 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag
     3721 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     3781 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     3841 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3901 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3961 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     4021 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     4081 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     4141 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     4201 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     4261 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     4321 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     4381 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     4441 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     4501 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     4561 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     4621 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     4681 catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat
     4741 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg
     4801 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     4861 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     4921 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc
     4981 aggttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     5041 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     5101 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5161 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5221 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5281 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5341 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5401 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5461 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5521 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5581 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5641 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5701 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5761 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     5821 taaccgtatt accgccatgc at
//