| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-CHD9-C-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001742 |
| 产品用途: | 3' AAVLINK plasmid for CHD9 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CHD9 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-CHD9-C-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-CHD9-C-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-CHD9-C-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-CHD9-C-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-CHD9-C-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-CHD9-C-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-CHD9-C-FLAG 7158 bp DNA circular SYN 22-JUL-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7158)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7158
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..141
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..141
/label=AAV2 ITR
misc_feature 156..236
/label=SCP1
CDS 248..1276
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1292..1381
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1306..1311
/label=SDS
misc_feature 1309..1314
/label=SDS
intron 1385..1425
/label=Splicing Donor
protein_bind 1432..1465
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1466..1557
/label=Splicing Acceptor
/note="Splicing acceptor"
misc_feature 1558..3871
/label=CHD9
misc_feature 1731..1736
/label=SDS
misc_feature 1734..1739
/label=SDS
misc_feature 1737..1742
/label=SDS
misc_feature 1740..1745
/label=SDS
misc_feature 2480..2485
/label=SDS
misc_feature 2559..2564
/label=SDS
misc_feature 3745..3750
/label=SDS
CDS 3878..3943
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3947..4378
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4091..4096
/label=SDS
repeat_region 4421..4561
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4421..4550
/label=AAV2 ITR
rep_origin 4636..5091
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5373..5477
/gene="bla"
/label=AmpR promoter
CDS 5478..6338
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6509..7097
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtgtact tatataaggg ggtgggggcg
181 cgttcgtcct cagtcgcgat cgaacactcg agccgagcag acgtgcctac ggaccgcgta
241 cgccaccatg tccaatttac tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc
301 aacgagtgat gaggttcgca agaacctgat ggacatgttc agggatcgcc aggcgttttc
361 tgagcatacc tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt
421 gaataaccgg aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct
481 tcaggcgcgc ggtctggcag taaaaactat ccagcaacat ttgggccagc taaacatgct
541 tcatcgtcgg tccgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg
601 gcggatccga aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga
661 acgcactgat ttcgaccagg ttcgttcact catggaaaat agcgatcgct gccaggatat
721 acgtaatctg gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc
781 caggatcagg gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg
841 cagaacgaaa acgctggtta gcaccgcagg tgtagagaag gcacttagcc tgggggtaac
901 taaactggtc gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct
961 gttttgccgg gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac
1021 tcgcgccctg gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggatga
1081 ctctggtcag agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga
1141 tatggcccgc gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt
1201 aaatattgta atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct
1261 gctggaagat ggcgatggag gtggatctgg tctgctgctg aaactcctcc tcctcggtgg
1321 tggttctggt cctcgtcccc gtggtactcg tggtaaaggt cgccgtattc actacaaagg
1381 ataagtaagt atcaaggtta caagacaggt ttaaggagac caataactag tataacttcg
1441 tataggatac tttatagcaa tttatgaaac tgggcttgtc gagacagaga agactcttgc
1501 gtttctgata ggcacctatt ggtcttactg acatccactt tgcctttctc tccacagggg
1561 atcagaatct agttctgatt ctgactcaga ttctgagaga tcatcttgtt cttccagatc
1621 atcttcttcc tcatcatcct cttcttgctc ccactctcga tcaggctcta gttcttcttc
1681 atcttcatct tgttcttcag catcttcttc atcctcttcc tccacctctt cctcctcctc
1741 ctcctcttca tcttcatcag aagaaagtga cagtgatgaa gaagaagccc aaaaacgaga
1801 aagtactact cacatgaaag cctatgatga agaaagcgtc gcgtcactga gcactaccca
1861 ggatgagact caggatagtt ttcagatgaa caatgggaca ccagagtctg cttatatctt
1921 acaaggtgga tatatgctgg cagcctcgta ttggccaaag gatcgtgtga tgatcaatag
1981 gttggacagt atttgtcaaa cagttctgaa aggaaagtgg ccttcagcta gaagaagtta
2041 tgatgctaac acagtggctt ctttctatac cacaaaactg ctggacagcc ctggagcagc
2101 tacagaatac agcgatccca gtgtacccac tcccccaggt gccggtgtta aagaagaaca
2161 tgatcagtca acacagatgt caaaggtgaa gaagcatgta cgagaaaagg agtttacagt
2221 gaaaatcaaa gacgaaggtg gtttgaagtt gacatttcag aagcaagggc ttgctcagaa
2281 aagaccattt gatggtgaag acggtgctct ggggcagcag cagtacctca ctcggcttcg
2341 agagcttcaa agtgcatcag agaccagcct cgtcaatttc ccaaaatcca taccagtatc
2401 aggtacttcc attcaaccaa cccttggtgc caatggtgtg atattagaca accagcctat
2461 agtcaaaaaa aggcgaggaa ggaggaagaa tgtagaaggt gttgacatct tcttttttaa
2521 cagaaataaa ccacctaatc atgtttcttt aggcttaacc tcctcacaga tttccacagg
2581 gataaatcca gcactatcct atactcaacc tcaaggaatt cctgatacag aaagtccagt
2641 tccagttatt aatcttaaag atggaacgag acttgcagga gatgatgcac caaagagaaa
2701 ggatttggaa aaatggctta aggagcaccc gggttatgtg gaagatttgg gagcttttat
2761 tcctagaatg cagcttcatg agggaagacc caaacaaaaa agacaccgtt gcagaaaccc
2821 caataaacta gatgtgaata gtctcactgg agaagaacgt gttcaactga ttaacagaag
2881 aaatgctaga aaggttggag gtgcatttgc tccccctttg aaagatttat gtagattcct
2941 aaaagaaaat tcagaatatg gagtagctcc tgaatgggga gatgttgtta agcaatctgg
3001 atttcttcca gaaagcatgt atgaacgtat tctcactggt cccgttgtga gagaggaagt
3061 aagcaggcgg gggagacggc ctaaaagtgg aattgcaaag gccacagcag cagcagctgc
3121 tgcatctgcc accagtgttt caggcaatcc tttgttagcc aatggactac ttccaggtgt
3181 ggatctcaca actcttcagg ccttacaaca aaacctacaa aacttgcagt cactgcaagt
3241 aactgctggg ttgatgggaa tgcctaccgg ccttccttct ggaggagaag ctaaaaacat
3301 ggctgctatg ttccccatgc tgctgtcagg aatggctgga ttaccaaatc tgttgggcat
3361 gggaggactc ctgacaaagc ctacggaatc tgggacagaa gacaaaaagg gaagtgactc
3421 taaggagtca gaaggaaaaa cagaaaggac agagagccaa agttcagaga atggtggaga
3481 aaactctgtg tcaagttctc cttccacatc ctctactgct gcattaaata cagctgcagc
3541 tgccaaccca ttagctctta acccactatt actatctaat atactttatc cagggatgct
3601 tctcactcca ggccttaatc ttcatattcc aactttgtcc cagtccaata cttttgatgt
3661 acaaaacaaa aacagtgact taggctcgtc taagtctgta gaagtaaaag aagaagattc
3721 cagaattaaa gatcaggaag acaaaggagg aactgaacca agtcctctca atgaaaacag
3781 cacagatgag ggttcagaga aagctgatgc ttcatctgga tctgatagta catcgtcgtc
3841 atctgaggat tcagattcta gtaatgaaga ctgtacagac tacaaagacc atgacggtga
3901 ttataaagat catgacatcg actacaagga tgacgatgac aagtaactcg agataatcaa
3961 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
4021 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
4081 ttcattttct cctccttgta taaatcctgg ttagttcttg ccacggcgga actcatcgcc
4141 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
4201 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccatctagct ttatttgtga
4261 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
4321 caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag
4381 atctggagac cggtgtggca cgtgcggacc gagcggccgc aggaacccct agtgatggag
4441 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
4501 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag
4561 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
4621 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
4681 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
4741 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
4801 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
4861 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
4921 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct
4981 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
5041 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
5101 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
5161 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
5221 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
5281 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
5341 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
5401 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
5461 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
5521 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
5581 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
5641 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
5701 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
5761 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
5821 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
5881 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
5941 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
6001 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
6061 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
6121 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
6181 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
6241 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
6301 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
6361 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
6421 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
6481 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
6541 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
6601 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
6661 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
6721 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
6781 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
6841 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
6901 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
6961 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
7021 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
7081 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
7141 ggccttttgc tcacatgt
//
