pQL72

LOCUS       pQL72             6502 bp DNA     circular SYN 22-APR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6502)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    238..329
                     /label=GGAA23
     misc_feature    336..483
                     /label=hcmvmin
     misc_feature    405..419
                     /label=New Feature
     primer_bind     complement(468..483)
                     /label=oYW11
     misc_feature    506..529
                     /label=New Feature
     primer_bind     530..556
                     /label=Oscl13-1
     misc_feature    531..2051
                     /label=SEAP
     primer_bind     complement(2031..2059)
                     /label=Oscl23-2
     primer_bind     complement(2033..2051)
                     /label=Oscl13-2
     terminator      2099..2326
                     /label=bGH PA
     misc_feature    2994..3071
                     /label=SV40 ORI
     CDS             3213..4004
                     /label=Kan/neoR
     primer_bind     complement(4344..4364)
                     /label=M13-rev
     misc_binding    complement(4370..4392)
                     /label=LacO
     promoter        complement(4397..4426)
                     /label=lac
     CDS             complement(5509..6168)
                     /label=AmpR
     promoter        complement(6408..6436)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgtacgga
      241 aggaaggaag gaaggaagga aggaaggaag gaaggaagga aggaaggaag gaaggaagga
      301 aggaaggaag gaaggaagga aggaaggaag ctagctcgag ctcggtaccc gggtcgagta
      361 ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct
      421 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc
      481 gcggccccga attcgagctc gcccggggat cctctagagt cagcttctgc atgctgctgc
      541 tgctgctgct gctgggcctg aggctacagc tctccctggg catcatccca gttgaggagg
      601 agaacccgga cttctggaac cgcgaggcag ccgaggccct gggtgccgcc aagaagctgc
      661 agcctgcaca gacagccgcc aagaacctca tcatcttcct gggcgatggg atgggggtgt
      721 ctacggtgac agctgccagg atcctaaaag ggcagaagaa ggacaaactg gggcctgaga
      781 tacccctggc catggaccgc ttcccatatg tggctctgtc caagacatac aatgtagaca
      841 aacatgtgcc agacagtgga gccacagcca cggcctacct gtgcggggtc aagggcaact
      901 tccagaccat tggcttgagt gcagccgccc gctttaacca gtgcaacacg acacgcggca
      961 acgaggtcat ctccgtgatg aatcgggcca agaaagcagg gaagtcagtg ggagtggtaa
     1021 ccaccacacg agtgcagcac gcctcgccag ccggcaccta cgcccacacg gtgaaccgca
     1081 actggtactc ggacgccgac gtgcctgcct cggcccgcca ggaggggtgc caggacatcg
     1141 ctacgcagct catctccaac atggacattg acgtgatcct aggtggaggc cgaaagtaca
     1201 tgtttcccat gggaacccca gaccctgagt acccagatga ctacagccaa ggtgggacca
     1261 ggctggacgg gaagaatctg gtgcaggaat ggctggcgaa gcgccagggt gcccggtatg
     1321 tgtggaaccg cactgagctc atgcaggctt ccctggaccc gtctgtgacc catctcatgg
     1381 gtctctttga gcctggagac atgaaatacg agatccaccg agactccaca ctggacccct
     1441 ccctgatgga gatgacagag gctgccctgc gcctgctgag caggaacccc cgcggcttct
     1501 tcctcttcgt ggagggtggt cgcatcgacc atggtcatca tgaaagcagg gcttaccggg
     1561 cactgactga gacgatcatg ttcgacgacg ccattgagag ggcgggccag ctcaccagcg
     1621 aggaggacac gctgagcctc gtcactgccg accactccca cgtcttctcc ttcggaggct
     1681 accccctgcg agggagctcc atcttcgggc tggcccctgg caaggcccgg gacaggaagg
     1741 cctacacggt cctcctatac ggaaacggtc caggctatgt gctcaaggac ggcgcccggc
     1801 cggatgttac cgagagcgag agcgggagcc ccgagtatcg gcagcagtca gcagtgcccc
     1861 tggacgaaga gacccacgca ggcgaggacg tggcggtgtt cgcgcgcggc ccgcaggcgc
     1921 acctggttca cggcgtgcag gagcagacct tcatagcgca cgtcatggcc ttcgccgcct
     1981 gcctggagcc ctacaccgcc tgcgacctgg cgccccccgc cggcaccacc gacgccgcgc
     2041 acccgggtta agcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg
     2101 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     2161 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     2221 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     2281 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     2341 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     2401 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     2461 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     2521 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     2581 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     2641 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     2701 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     2761 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     2821 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     2881 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     2941 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     3001 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     3061 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     3121 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     3181 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     3241 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     3301 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     3361 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     3421 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     3481 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     3541 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     3601 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     3661 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     3721 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     3781 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     3841 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     3901 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     3961 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     4021 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     4081 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     4141 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     4201 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     4261 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     4321 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     4381 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     4441 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     4501 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     4561 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     4621 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     4681 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     4741 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     4801 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     4861 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     4921 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     4981 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     5041 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     5101 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     5161 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     5221 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     5281 tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     5341 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     5401 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     5461 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     5521 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     5581 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     5641 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     5701 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     5761 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     5821 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     5881 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     5941 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     6001 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     6061 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     6121 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     6181 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     6241 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     6301 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     6361 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     6421 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     6481 ccgaaaagtg ccacctgacg tc
//