| 质粒名称: | pMS82 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 68110 |
| 存储实验室: | Maggie Smith |
|
载体骨架基本信息 |
|
| 载体名称: | pSET152 |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 6116 bp |
| 载体类型: | Streptomyces integrating vector |
| 载体抗性: | Hygromycin, 200 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称1: | aac3(IV)、apramycin resistance (fragment) |
| 物种来源: | E. coli |
| 插入大小: | 26 bp |
| 基因突变: | Fragment |
| 插入基因名称2: | Hyg、Hygromycin resistance |
| 插入大小: | 998bp |
| 插入基因名称3: | oriT、origin of transfer |
| 物种来源: | E. coli |
| 插入大小: | 111bp |
| 插入基因名称4: | attP、phage attachment site |
| 物种来源: | bacteriophage phiBT1 |
| 插入大小: | 51bp |
| 插入基因名称5: | int、phiBT1 Integrase |
| 物种来源: | bacteriophage phiBT1 |
| 插入大小: | 1785bp |
|
文献引用方法 |
|
| 材料和方法部分: | pMS82 was a gift from Maggie Smith (Addgene plasmid # 68110 ; http://n2t.net/addgene:68110 ; RRID:Addgene_68110) |
| 参考文献部分: | Integration site for Streptomyces phage phiBT1 and development of site-specific integrating vectors. Gregory MA, Till R, Smith MC. J Bacteriol. 2003 Sep;185(17):5320-3. PubMed 12923110 |
买家导航
pMS82载体质粒基本信息
pMS82质粒图谱载体图谱和pMS82载体序列质粒序列多克隆位点信息
pMS82质粒载体简介
pMS82质粒序列载体序列
LOCUS pMS82 6116 bp DNA circular SYN 25-AUG-2024
DEFINITION Integration vector derived from Streptomyces phage phiBT1.
Hygromycin resistance gene, oriT, ColEi origin of replication.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6116)
AUTHORS Gregory MA, Till R, Smith MC
TITLE Integration site for Streptomyces phage phiBT1 and development of
site-specific integrating vectors.
JOURNAL J Bacteriol. 2003 Sep;185(17):5320-3.
PUBMED 12923110
REFERENCE 2 (bases 1 to 6116)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J
Bacteriol."; date: "2003-09"; volume: "185(17)"; pages: "5320-3"
FEATURES Location/Qualifiers
source 1..6116
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(38..54)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(38..54)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(51..73)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 62..78
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(86..116)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 131..152
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(269..286)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(440..1028)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(520..539)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3416..3787)
/codon_start=1
/gene="traJ"
/product="oriT-recognizing protein"
/label=traJ
/translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
KQDELGKVMMGVVRPRAEP"
oriT 3820..3929
/note="incP origin of transfer"
ORIGIN
1 gatccgcggc cgcgcgcgat atcgaattcg taatcatgtc atagctgttt cctgtgtgaa
61 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
121 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
181 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
241 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
301 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
361 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
421 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
481 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
541 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
601 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
661 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
721 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
781 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
841 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
901 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
961 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
1021 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
1081 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttgg
1141 ttcatgtgca gctccatcag caaaagggga tgataagttt atcaccaccg actatttgca
1201 acagtgccgt tgatcgtgct atgatcgact gatgtcatca gcggtggagt gcaatgtcgt
1261 gcaatacgaa tggcgaaaag ccgagaacct aggatccaag cttagatcta tgcatggtac
1321 cagtgagcgt ttttcaacct caggctgacg gggtcagggg tcagaggcga ggtaaccatc
1381 tgcggtgata aattatctct ggcggtgttg acataaatac cactggcggt gatactgagc
1441 acatcagatc ggtcgtcgag gtccacaggc cctcggcgac gttccgccgg gcctcggcga
1501 ccgccgcgtc gaggcgccgg tcgaaggggc agtcctccac gggcagctcg tggagggcgc
1561 gggccagctc cgccatcgcc tcgaccacgg cgaaccgctg gtgctcgggc cactcctcgg
1621 ccgccgcgac gccggggacg gcctccgtga cgagccacgc ggcggtgtcg tcggcaccgc
1681 gctcgacgac gcgggggacg gggatcggcg gggcctggga cggcgcctcg ccgtcgcaga
1741 accaggcggt ggcgtacacc gtcgcctcgg tcggcccgta gagattggcg atcccgaccg
1801 cagcaccacc gagaacgtcc ccgacgtggc cgaccagccc gtcatcgtca acgcctgatc
1861 cgcggtgcgg acaggccgtg tcgcgaccgg ccgtgcggaa ttaagccggc ccgtaccctg
1921 tgaatagagg tccgctgtga cacaagaatc cctgttactt ctcgaccgta ttgattcgga
1981 tgattcctac gcgagcctgc ggaacgacca ggaattctgg gagccgctgg cccgccgagc
2041 cctggaggag ctcgggctgc cggtgccgcc ggtgctgcgg gtgcccggcg agagcaccaa
2101 ccccgtactg gtcggcgagc ccggcccggt gatcaagctg ttcggcgagc actggtgcgg
2161 tccggagagc ctcgcgtcgg agtcggaggc gtacgcggtc ctggcggacg ccccggtgcc
2221 ggtgccccgc ctcctcggcc gcggcgagct gcggcccggc accggagcct ggccgtggcc
2281 ctacctggtg atgagccgga tgaccggcac cacctggcgg tccgcgatgg acggcacgac
2341 cgaccggaac gcgctgctcg ccctggcccg cgaactcggc cgggtgctcg gccggctgca
2401 cagggtgccg ctgaccggga acaccgtgct caccccccat tccgaggtct tcccggaact
2461 gctgcgggaa cgccgcgcgg cgaccgtcga ggaccaccgc gggtggggct acctctcgcc
2521 ccggctgctg gaccgcctgg aggactggct gccggacgtg gacacgctgc tggccggccg
2581 cgaaccccgg ttcgtccacg gcgacctgca cgggaccaac atcttcgtgg acctggccgc
2641 gaccgaggtc accgggatcg tcgacttcac cgacgtctat gcgggagact cccgctacag
2701 cctggtgcaa ctgcatctca acgccttccg gggcgaccgc gagatcctgg ccgcgctgct
2761 cgacggggcg cagtggaagc ggaccgagga cttcgcccgc gaactgctcg ccttcacctt
2821 cctgcacgac ttcgaggtgt tcgaggagac cccgctggat ctctccggct tcaccgatcc
2881 ggaggaactg gcgcagttcc tctgggggcc gccggacacc gcccccggcg cctgacgccc
2941 cgggtgaaga gccccgtcgg gcggtgcctg acggggcttc tcagttctca tgagcggaga
3001 acgagatgac gttggagggg caaggtcgcg ctgattgctg gggcaacacg tggagcggat
3061 cggggattgt ctttcttcag ctcgctgatg atatgctgac gctcaatgcc gtttggcctc
3121 cgactaacga aaatcccgca tttggacggc tgatccgatt ggcacggcgg acggcgaatg
3181 gcggagcaga cgctcgtccg ggggcaatga gatatgaaaa agcctgaact caccgcgacg
3241 tatcgggccc tggccagcta gctagagtcg acctgcaggt ccccggggat cggtcttgcc
3301 ttgctcgtcg gtgatgtact tcaccagctc cgcgaagtcg ctcttcttga tggagcgcat
3361 ggggacgtgc ttggcaatca cgcgcacccc ccggccgttt tagcggctaa aaaagtcatg
3421 gctctgccct cgggcggacc acgcccatca tgaccttgcc aagctcgtcc tgcttctctt
3481 cgatcttcgc cagcagggcg aggatcgtgg catcaccgaa ccgcgccgtg cgcgggtcgt
3541 cggtgagcca gagtttcagc aggccgccca ggcggcccag gtcgccattg atgcgggcca
3601 gctcgcggac gtgctcatag tccacgacgc ccgtgatttt gtagccctgg ccgacggcca
3661 gcaggtaggc cgacaggctc atgccggccg ccgccgcctt ttcctcaatc gctcttcgtt
3721 cgtctggaag gcagtacacc ttgataggtg ggctgccctt cctggttggc ttggtttcat
3781 cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct cgcagagcag
3841 gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg
3901 cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt
3961 ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa
4021 aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaagatccgt cgacctgcag
4081 gcatgctggc gccggacggg gcttcagacg tttcgggtgc tgggttgttg tctctggaca
4141 gtgatccatg ggaaactact cagcaccacc aatgttccca aaagaaagcg caggtcagcg
4201 cccatgagcc aagatctagg catgtcgccc ttcatcgctc ccgacgtccc tgagcacctt
4261 ctagacactg ttcgcgtctt cctgtacgcg cgtcagtcta agggccggtc cgacggctca
4321 gacgtgtcga ccgaagcaca gctagcggcc ggtcgtgcgt tggtcgcgtc tcgcaacgcc
4381 caggggggtg cgcgctgggt cgtggcaggt gagttcgtgg acgtcgggcg ctccggctgg
4441 gacccgaacg tgacccgtgc cgacttcgag cgcatgatgg gcgaagtccg cgccggcgaa
4501 ggtgacgttg tcgttgtgaa tgagctttcc cggctcactc gcaagggcgc ccatgacgcg
4561 ctcgaaatcg acaacgaatt gaagaagcac ggcgtgcgct tcatgtcggt tcttgagccg
4621 ttccttgaca cgtctacccc tatcggcgtc gccattttcg cgctgatcgc tgcccttgcg
4681 aaacaggaca gtgacctgaa ggcggagcgc ctgaagggtg cgaaagacga gattgccgcg
4741 ctgggtggcg ttcactcgtc ttccgccccg ttcggaatgc gcgccgtgcg caagaaggtc
4801 gataatctcg tgatctccgt tcttgagccg gacgaagaca acccggatca cgtcgagcta
4861 gttgagcgca tggcgaaaat gtcgttcgaa ggcgtgtccg acaacgccat tgcaacgacc
4921 ttcgagaagg aaaagatccc gtcgcccgga atggctgaga gacgcgccac ggaaaagcgt
4981 cttgcgtcca tcaaggcacg tcgcctgaac ggcgctgaaa agccgatcat gtggcgcgct
5041 caaacggtcc gatggattct caaccatccc gcaatcggcg gtttcgcatt cgagcgtgtg
5101 aagcacggta aggcgcacat caacgtcata cggcgcgacc ccggcggcaa gccgctaacg
5161 ccccacacgg gcattctcag cggctcgaag tggcttgagc ttcaagagaa gcgttccggg
5221 aagaatctca gcgaccggaa gcctggggcc gaagtcgaac cgacgcttct gagcgggtgg
5281 cgtttcctgg ggtgccgaat ctgcggcggc tcaatgggtc agtcccaggg tggccgtaag
5341 cgcaacggcg accttgccga aggcaattac atgtgcgcca acccgaaggg gcacggcggc
5401 ttgtcggtca agcgcagcga actggacgaa ttcgttgctt cgaaggtgtg ggcacggctc
5461 cgcacagccg acatggaaga tgaacacgat caggcatgga ttgccgccgc tgcggagcgc
5521 ttcgcccttc agcacgacct agcgggggtg gccgatgagc ggcgcgaaca acaggcgcac
5581 ctagacaacg tgcggcgctc catcaaggac cttcaggcgg accgtaaggc cggtctgtac
5641 gtcgggcgtg aagagctgga aacgtggcgc tcaacggtgc tgcaataccg gtcctacgaa
5701 gcggagtgca cgacccgact cgctgagctt gacgagaaga tgaacggcag cacccgcgtt
5761 ccgtctgagt ggttcagcgg cgaagacccg acggccgaag ggggcatctg ggcaagctgg
5821 gacgtgtacg agcgtcggga gttcctgagc ttcttccttg actccgtcat ggtcgaccgg
5881 gggcgccacc ctgagacgaa gaaatacatc cccctgaagg accgtgtgac gctcaagtgg
5941 gcggagctgc tgaaggagga agacgaagcg agcgaagcca ctgagcggga gcttgcggcg
6001 ctgtagcgca cagcgggagg ggtcgagccg gcggacggtt cggccccttt tttggccttg
6061 aaatcgttag ttaggctaac tagtagttcc ttcgtcacca tagcgggcag ggagcg
//
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