KA1153_pPBCAG-MerCreMer-IN

LOCUS       KA1153_pPBCAG-MerCreMer-IN                9704 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  piggyBac-based expression of the tamoxifen-inducible Cre.
ACCESSION   .
VERSION     .
KEYWORDS    KA1153_pPBCAG-MerCreMer-IN
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9704)
  AUTHORS   Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, 
            Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
  TITLE     Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive 
            Pluripotency.
  JOURNAL   Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 
            10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
  PUBMED    29910149
REFERENCE   2  (bases 1 to 9704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9704
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   660..694
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1052..1355
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1360..1637
                     /label=chicken beta-actin promoter
     intron          1638..2654
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2578..2600
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2662..2681
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3737..3757
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3758..4783
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     misc_feature    5819..6405
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5986..6003)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6213..6232
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6406..7200
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6460..6479)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7070..7089
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7207..7255
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     repeat_region   7430..7492
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(7516..7534)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7555..7571)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7555..7571)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7568..7590)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7579..7595
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7603..7633)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7648..7669
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7786..7803)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7957..8545)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8037..8056)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8716..9576)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9339..9358
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9577..9681)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccttaac
      661 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt
      721 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc
      781 cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg
      841 agtcaaaatg acgcatgatt atcttttacg tgaattttaa gatttaactc atacgataat
      901 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta
      961 tagatatcgt cgacattgat tattgactag ctagttatta atagtaatca attacggggt
     1021 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     1081 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     1141 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     1201 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     1261 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     1321 agtacatcta cgtattagtc atcgctatta ccatgatggt cgaggtgagc cccacgttct
     1381 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
     1441 aattattttg tgcagcgatg ggggcggggg gggggggggg gcgcgcgccr ggsggggsgg
     1501 ggsggggsgr ggggsggggs ggggsgrggc ggagaggtgc ggcggcagcc aatcagagcg
     1561 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc
     1621 gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg
     1681 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc
     1741 gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt
     1801 ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg
     1861 gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc
     1921 ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg
     1981 aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag
     2041 gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc
     2101 tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg
     2161 ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg
     2221 gggtgccggg cggggcgggg ccgcctcggg ccggggrggg ctcgggggag gggcgcggcg
     2281 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
     2341 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg
     2401 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga
     2461 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc
     2521 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt
     2581 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct
     2641 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca
     2701 aagaattcct cgagatctca cgcgtgacta gcttgcatgc ctgcacacca tgggagatcc
     2761 acgaaatgaa atgggtgctt caggagacat gagggctgcc aacctttggc caagccctct
     2821 tgtgattaag cacactaaga agaatagccc tgccttgtcc ttgacagctg accagatggt
     2881 cagtgccttg ttggatgctg aaccgcccat gatctattct gaatatgatc cttctagacc
     2941 cttcagtgaa gcctcaatga tgggcttatt gaccaaccta gcagataggg agctggttca
     3001 tatgatcaac tgggcaaaga gagtgccagg ctttggggac ttgaatctcc atgatcaggt
     3061 ccaccttctc gagtgtgcct ggctggagat tctgatgatt ggtctcgtct ggcgctccat
     3121 ggaacacccg gggaagctcc tgtttgctcc taacttgctc ctggacagga atcaaggtaa
     3181 atgtgtggaa ggcatggtgg agatctttga catgttgctt gctacgtcaa gtcggttccg
     3241 catgatgaac ctgcagggtg aagagtttgt gtgcctcaaa tccatcattt tgcttaattc
     3301 cggagtgtac acgtttctgt ccagcacctt gaagtctctg gaagagaagg accacatcca
     3361 ccgtgtcctg gacaagatca cagacacttt gatccacctg atggccaaag ctggcctgac
     3421 tctgcagcag cagcatcgcc gcctagctca gctccttctc attctttccc atatccggca
     3481 tatgagtaac aaacgcatgg agcatctcta caacatgaaa tgcaagaacg tggtacccct
     3541 ctatgacctg ctcctggaga tgttggatgc ccaccgcctt catgccccag ccagtcgcat
     3601 gggagtgccc ccagaggagc ccagccagac ccagctggcc accaccagct ccacttcagc
     3661 acattcctta caaacctact acataccccc ggaagcagag ggcttcccca acacgatcgt
     3721 cgacctgcag accatgccca agaagaagag gaaggtgtcc aatttactga ccgtacacca
     3781 aaatttgcct gcattaccgg tcgatgcaac gagtgatgag gttcgcaaga acctgatgga
     3841 catgttcagg gatcgccagg cgttttctga gcatacctgg aaaatgcttc tgtccgtttg
     3901 ccggtcgtgg gcggcatggt gcaagttgaa taaccggaaa tggtttcccg cagaacctga
     3961 agatgttcgc gattatcttc tatatcttca ggcgcgcggt ctggcagtaa aaactatcca
     4021 gcaacatttg ggccagctaa acatgcttca tcgtcggtcc gggctgccac gaccaagtga
     4081 cagcaatgct gtttcactgg ttatgcggcg gatccgaaaa gaaaacgttg atgccggtga
     4141 acgtgcaaaa caggctctag cgttcgaacg cactgatttc gaccaggttc gttcactcat
     4201 ggaaaatagc gatcgctgcc aggatatacg taatctggca tttctgggga ttgcttataa
     4261 caccctgtta cgtatagccg aaattgccag gatcagggtt aaagatatct cacgtactga
     4321 cggtgggaga atgttaatcc atattggcag aacgaaaacg ctggttagca ccgcaggtgt
     4381 agagaaggca cttagcctgg gggtaactaa actggtcgag cgatggattt ccgtctctgg
     4441 tgtagctgat gatccgaata actacctgtt ttgccgggtc agaaaaaatg gtgttgccgc
     4501 gccatctgcc accagccagc tatcaactcg cgccctggaa gggatttttg aagcaactca
     4561 tcgattgatt tacggcgcta aggatgactc tggtcagaga tacctggcct ggtctggaca
     4621 cagtgcccgt gtcggagccg cgcgagatat ggcccgcgct ggagtttcaa taccggagat
     4681 catgcaagct ggtggctgga ccaatgtaaa tattgtcatg aactatatcc gtaacctgga
     4741 tagtgaaaca ggggcaatgg tgcgcctgct ggaagatggc gatggaggtt ctggagatcc
     4801 acgaaatgaa atgggtgctt caggagacat gagggctgcc aacctttggc caagccctct
     4861 tgtgattaag cacactaaga agaatagccc tgccttgtcc ttgacagctg accagatggt
     4921 cagtgccttg ttggatgctg aaccgcccat gatctattct gaatatgatc cttctagacc
     4981 cttcagtgaa gcctcaatga tgggcttatt gaccaaccta gcagataggg agctggttca
     5041 tatgatcaac tgggcaaaga gagtgccagg ctttggggac ttgaatctcc atgatcaggt
     5101 ccaccttctc gagtgtgcct ggctggagat tctgatgatt ggtctcgtct ggcgctccat
     5161 ggaacacccg gggaagctcc tgtttgctcc taacttgctc ctggacagga atcaaggtaa
     5221 atgtgtggaa ggcatggtgg agatctttga catgttgctt gctacgtcaa gtcggttccg
     5281 catgatgaac ctgcagggtg aagagtttgt gtgcctcaaa tccatcattt tgcttaattc
     5341 cggagtgtac acgtttctgt ccagcacctt gaagtctctg gaagagaagg accacatcca
     5401 ccgtgtcctg gacaagatca cagacacttt gatccacctg atggccaaag ctggcctgac
     5461 tctgcagcag cagcatcgcc gcctagctca gctccttctc attctttccc atatccggca
     5521 tatgagtaac aaacgcatgg agcatctcta caacatgaaa tgcaaaaacg tggtacccct
     5581 ctatgacctg ctcctggaga tgttggatgc ccaccgcctt catgccccag ccagtcgcat
     5641 gggagtgccc ccagaggagc ccagccagac ccagctggcc accaccagct ccacttcagc
     5701 acattcctta caaacctact acataccccc ggaagcagag ggcttcccca acacgatctg
     5761 agagctccta ctagctgagc ggccgctcga taagcttgat atcgaattcc gccccccccc
     5821 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     5881 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     5941 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     6001 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     6061 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     6121 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     6181 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     6241 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     6301 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     6361 acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatgat tgaacaagat
     6421 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     6481 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     6541 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     6601 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     6661 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     6721 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     6781 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     6841 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     6901 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     6961 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     7021 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     7081 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     7141 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     7201 ggggatcggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttcggatc
     7261 ctttgttact ttatagaaga aattttgagt ttttgttttt ttttaataaa taaataaaca
     7321 taaataaatt gtttgttgaa tttattatta gtatgtaagt gtaaatataa taaaacttaa
     7381 tatctattca aattaataaa taaacctcga tatacagacc gataaaacac atgcgtcaat
     7441 tttacgcatg attatcttta acgtacgtca caatatgatt atctttctag ggttaaagag
     7501 ctccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata
     7561 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7621 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7681 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7741 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7801 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7861 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7921 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7981 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8041 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8101 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8161 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8221 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8281 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8341 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     8401 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8461 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8521 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8581 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8641 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8701 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8761 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8821 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8881 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8941 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9001 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9061 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9121 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9181 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9241 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9301 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9361 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9421 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9481 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9541 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9601 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9661 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccac
//