| 质粒名称: | pdCas9-PBE |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 98161 |
| 存储实验室: | Caixia Gao |
|
载体骨架基本信息 |
|
| 载体名称: | pJIT163-Ubi-GFP |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 植物表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | NLS-APOBEC1-XTEN-dCas9-UGI-NLS |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | Ubi |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI (未破坏) |
| 3' 克隆位点: | Bsp1407I (未破坏) |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pdCas9-PBE was a gift from Caixia Gao (Addgene plasmid # 98161 ; http://n2t.net/addgene:98161 ; RRID:Addgene_98161) |
| 参考文献部分: | Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Zong Y, Wang Y, Li C, Zhang R, Chen K, Ran Y, Qiu JL, Wang D, Gao C. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. 10.1038/nbt.3811 PubMed 28244994 |
买家导航
pdCas9-PBE载体 pdCas9PBE质粒基本信息
pdCas9-PBE质粒图谱 pdCas9PBE载体图谱和pdCas9-PBE载体序列 pdCas9PBE质粒序列多克隆位点信息
pdCas9-PBE质粒 pdCas9PBE载体简介
pdCas9-PBE质粒序列 pdCas9PBE载体序列
LOCUS pdCas9-PBE 10201 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Expresses CRISPR base editor in protoplasts.
ACCESSION .
VERSION .
KEYWORDS pdCas9-PBE
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10201)
AUTHORS Zong Y, Wang Y, Li C, Zhang R, Chen K, Ran Y, Qiu JL, Wang D, Gao C
TITLE Precise base editing in rice, wheat and maize with a Cas9-cytidine
deaminase fusion.
JOURNAL Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub
2017 Feb 27.
PUBMED 28244994
REFERENCE 2 (bases 1 to 10201)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10201
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 2020..2040
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 2041..2724
/codon_start=1
/product="cytidine deaminase (C to U editing enzyme) from
rat"
/label=APOBEC-1
/note="can use ssDNA as a substrate (Komor et al., 2016)"
/translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
WATGLK"
CDS 2776..6876
/codon_start=1
/product="catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes Type II
CRISPR/Cas system"
/label=dCas9
/note="RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in the RuvC
catalytic domain and the H840A mutation in the HNH
catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 6877..6924
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 6946..7194
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 7207..7227
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 7301..7477
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
primer_bind complement(8031..8047)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8031..8047)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8044..8066)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8055..8071
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8079..8109)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8124..8145
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8262..8279)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8433..9021)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8513..8532)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9192..10052)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9815..9834
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10053..10157)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gagctcggta cctgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa
61 gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat
121 ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat
181 atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag
241 tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt
301 tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg
361 tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta
421 gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat
481 aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa
541 actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac
601 gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac
661 ggcacggcat ctctgtcgct gcctctggac ccctctcgat cgagagttcc gctccaccgt
721 tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg
781 cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc
841 accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc
901 tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat
961 ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc cccctctcta
1021 ccttctctag atcggcgttc cggtccatgg ttagggcccg gtagttctac ttctgttcat
1081 gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta cacggatgcg
1141 acctgtacgt cagacacgtt ctgattgcta acttgccagt gtttctcttt ggggaatcct
1201 gggatggctc tagccgttcc gcagacggga tcgatttcat gatttttttt gtttcgttgc
1261 atagggtttg gtttgccctt ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg
1321 tcatcttttc atgctttttt ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt
1381 tctagatcgg agtagaatta attctgtttc aaactacctg gtggatttat taattttgga
1441 tctgtatgtg tgtgccatac atattcatag ttacgaattg aagatgatgg atggaaatat
1501 cgatctagga taggtataca tgttgatgcg ggttttactg atgcatatac agagatgctt
1561 tttgttcgct tggttgtgat gatgtggtgt ggttgggcgg tcgttcattc gttctagatc
1621 ggagtagaat actgtttcaa actacctggt gtatttatta attttggaac tgtatgtgtg
1681 tgtcatacat cttcatagtt acgagtttaa gatggatgga aatatcgatc taggataggt
1741 atacatgttg atgtgggttt tactgatgca tatacatgat ggcatatgca gcatctattc
1801 atatgctcta accttgagta cctatctatt ataataaaca agtatgtttt ataattattt
1861 tgatcttgat atacttggat gatggcatat gcagcagcta tatgtggatt tttttagccc
1921 tgccttcata cgctatttat ttgcttggta ctgtttcttt tgtcgatgct caccctgttg
1981 tttggtgtta cttctgcaaa gcttgtcgac ggatccatgc caaagaagaa gaggaaggtt
2041 tcatcggaga ccggccctgt tgctgttgac cccaccctgc ggcggagaat cgagccacac
2101 gagttcgagg tgttcttcga cccaagggag ctccgcaagg agacgtgcct cctgtacgag
2161 atcaactggg gcggcaggca ctccatctgg aggcacacca gccaaaacac caacaagcac
2221 gtggaggtca acttcatcga gaagttcacc accgagaggt acttctgccc aaacacccgc
2281 tgctccatca cctggttcct gtcctggagc ccatgcggcg agtgctccag ggccatcacc
2341 gagttcctca gccgctaccc acacgtcacc ctgttcatct acatcgccag gctctaccac
2401 cacgccgacc caaggaacag gcagggcctc cgcgacctga tctccagcgg cgtgaccatc
2461 caaatcatga ccgagcagga gtccggctac tgctggagga acttcgtcaa ctactcccca
2521 agcaacgagg cccactggcc aaggtaccca cacctctggg tgcgcctcta cgtgctcgag
2581 ctgtactgca tcatcctcgg cctgccacca tgcctcaaca tcctgaggcg caagcaacca
2641 cagctgacct tcttcaccat cgccctccaa agctgccact accagaggct cccaccacac
2701 atcctgtggg ctaccggcct caagtccggc agcgagacgc caggcacctc cgagagcgct
2761 acgcctgaac ttaaggacaa gaagtactcg atcggcctcg ccatcgggac gaactcagtt
2821 ggctgggccg tgatcaccga cgagtacaag gtgccctcta agaagttcaa ggtcctgggg
2881 aacaccgacc gccattccat caagaagaac ctcatcggcg ctctcctgtt cgacagcggg
2941 gagaccgctg aggctacgag gctcaagaga accgctaggc gccggtacac gagaaggaag
3001 aacaggatct gctacctcca agagattttc tccaacgaga tggccaaggt tgacgattca
3061 ttcttccacc gcctggagga gtctttcctc gtggaggagg ataagaagca cgagcggcat
3121 cccatcttcg gcaacatcgt ggacgaggtt gcctaccacg agaagtaccc tacgatctac
3181 catctgcgga agaagctcgt ggactccacc gataaggcgg acctcagact gatctacctc
3241 gctctggccc acatgatcaa gttccgcggc catttcctga tcgaggggga tctcaaccca
3301 gacaacagcg atgttgacaa gctgttcatc caactcgtgc agacctacaa ccaactcttc
3361 gaggagaacc cgatcaacgc ctctggcgtg gacgcgaagg ctatcctgtc cgcgaggctc
3421 tcgaagtcca ggaggctgga gaacctgatc gctcagctcc caggcgagaa gaagaacggc
3481 ctgttcggga acctcatcgc tctcagcctg gggctcaccc cgaacttcaa gtcgaacttc
3541 gatctcgctg aggacgccaa gctgcaactc tccaaggaca cctacgacga tgacctcgat
3601 aacctcctgg cccagatcgg cgatcaatac gcggacctgt tcctcgctgc caagaacctg
3661 tcggacgcca tcctcctgtc agatatcctc cgcgtgaaca ccgagatcac gaaggctcca
3721 ctctctgcct ccatgatcaa gcgctacgac gagcaccatc aggatctgac cctcctgaag
3781 gcgctggtcc gccaacagct cccggagaag tacaaggaga ttttcttcga tcagtcgaag
3841 aacggctacg ctgggtacat cgacggcggg gcctcacaag aggagttcta caagttcatc
3901 aagccaatcc tggagaagat ggacggcacg gaggagctcc tggtgaagct caacagggag
3961 gacctcctgc ggaagcagag aaccttcgat aacggcagca tcccccacca aatccatctc
4021 ggggagctgc acgccatcct gagaaggcaa gaggacttct accctttcct caaggataac
4081 cgggagaaga tcgagaagat cctgaccttc agaatcccat actacgtcgg ccctctcgcg
4141 cgggggaact caagattcgc ttggatgacc cgcaagtctg aggagaccat cacgccgtgg
4201 aacttcgagg aggtggtgga caagggcgct agcgctcagt cgttcatcga gaggatgacc
4261 aacttcgaca agaacctgcc caacgagaag gtgctcccta agcactcgct cctgtacgag
4321 tacttcaccg tctacaacga gctcacgaag gtgaagtacg tcaccgaggg catgcgcaag
4381 ccagcgttcc tgtccgggga gcagaagaag gctatcgtgg acctcctgtt caagaccaac
4441 cggaaggtca cggttaagca actcaaggag gactacttca agaagatcga gtgcttcgat
4501 tcggtcgaga tcagcggcgt tgaggaccgc ttcaacgcca gcctcgggac ctaccacgat
4561 ctcctgaaga tcatcaagga taaggacttc ctggacaacg aggagaacga ggatatcctg
4621 gaggacatcg tgctgaccct cacgctgttc gaggacaggg agatgatcga ggagcgcctg
4681 aagacgtacg cccatctctt cgatgacaag gtcatgaagc aactcaagcg ccggagatac
4741 accggctggg ggaggctgtc ccgcaagctc atcaacggca tccgggacaa gcagtccggg
4801 aagaccatcc tcgacttcct gaagagcgat ggcttcgcca acaggaactt catgcaactg
4861 atccacgatg acagcctcac cttcaaggag gatatccaaa aggctcaagt gagcggccag
4921 ggggactcgc tgcacgagca tatcgcgaac ctcgctggct cccccgcgat caagaagggc
4981 atcctccaga ccgtgaaggt tgtggacgag ctcgtgaagg tcatgggccg gcacaagcct
5041 gagaacatcg tcatcgagat ggccagagag aaccaaacca cgcagaaggg gcaaaagaac
5101 tctagggagc gcatgaagcg catcgaggag ggcatcaagg agctggggtc ccaaatcctc
5161 aaggagcacc cagtggagaa cacccaactg cagaacgaga agctctacct gtactacctc
5221 cagaacggca gggatatgta cgtggaccaa gagctggata tcaaccgcct cagcgattac
5281 gacgtcgatg ctatcgttcc ccagtctttc ctgaaggatg actccatcga caacaaggtc
5341 ctcaccaggt cggacaagaa ccgcggcaag tcagataacg ttccatctga ggaggtcgtt
5401 aagaagatga agaactactg gaggcagctc ctgaacgcca agctgatcac gcaaaggaag
5461 ttcgacaacc tcaccaaggc tgagagaggc gggctctcag agctggacaa ggccggcttc
5521 atcaagcggc agctggtcga gaccagacaa atcacgaagc acgttgcgca aatcctcgac
5581 tctcggatga acacgaagta cgatgagaac gacaagctga tcagggaggt taaggtgatc
5641 accctgaagt ctaagctcgt ctccgacttc aggaaggatt tccagttcta caaggttcgc
5701 gagatcaaca actaccacca tgcccatgac gcttacctca acgctgtggt cggcaccgct
5761 ctgatcaaga agtacccaaa gctggagtcc gagttcgtgt acggggacta caaggtttac
5821 gatgtgcgca agatgatcgc caagtcggag caagagatcg gcaaggctac cgccaagtac
5881 ttcttctact caaacatcat gaacttcttc aagaccgaga tcacgctggc caacggcgag
5941 atccggaaga gaccgctcat cgagaccaac ggcgagacgg gggagatcgt gtgggacaag
6001 ggcagggatt tcgcgaccgt ccgcaaggtt ctctccatgc cccaggtgaa catcgtcaag
6061 aagaccgagg tccaaacggg cgggttctca aaggagtcta tcctgcctaa gcggaacagc
6121 gacaagctca tcgccagaaa gaaggactgg gacccaaaga agtacggcgg gttcgacagc
6181 cctaccgtgg cctactcggt cctggttgtg gcgaaggttg agaagggcaa gtccaagaag
6241 ctcaagagcg tgaaggagct cctggggatc accatcatgg agaggtccag cttcgagaag
6301 aacccaatcg acttcctgga ggccaagggc tacaaggagg tgaagaagga cctgatcatc
6361 aagctcccga agtactctct cttcgagctg gagaacggca ggaagagaat gctggcttcc
6421 gctggcgagc tccagaaggg gaacgagctc gcgctgccaa gcaagtacgt gaacttcctc
6481 tacctggctt cccactacga gaagctcaag ggcagcccgg aggacaacga gcaaaagcag
6541 ctgttcgtcg agcagcacaa gcattacctc gacgagatca tcgagcaaat ctccgagttc
6601 agcaagcgcg tgatcctcgc cgacgcgaac ctggataagg tcctctccgc ctacaacaag
6661 caccgggaca agcccatcag agagcaagcg gagaacatca tccatctctt caccctgacg
6721 aacctcggcg ctcctgctgc tttcaagtac ttcgacacca cgatcgatcg gaagagatac
6781 acctccacga aggaggtcct ggacgcgacc ctcatccacc agtcgatcac cggcctgtac
6841 gagacgagga tcgacctctc acaactcggc ggggataaga gacccgcagc aaccaagaag
6901 gcagggcaag caaagaagaa gaagacgcgt gactccggcg gcagcaccaa cctgtccgac
6961 atcatcgaga aggagacggg caagcaactc gtgatccagg agagcatcct catgctgcca
7021 gaggaggtgg aggaggtcat cggcaacaag ccagagtccg acatcctggt gcacaccgcc
7081 tacgacgagt ccaccgacga gaacgtcatg ctcctgacca gcgacgcccc agagtacaag
7141 ccatgggccc tcgtcatcca ggacagcaac ggggagaaca agatcaagat gctgtcgggg
7201 gggagcccaa agaagaagcg gaaggtgtag tgtacaagta acccgggaat tcggtacgct
7261 gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata aataatgtgt
7321 gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat gtgttgagca
7381 tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta
7441 attcctaaaa ccaaaatcca gtactaaaat ccagatctcc taaagtccct atagatcttt
7501 gtcgtgaata taaaccagac acgagacgac taaacctgga gcccagacgc cgttcgaagc
7561 tagaagtacc gcttaggcag gaggccgtta gggaaaagat gctaaggcag ggttggttac
7621 gttgactccc ccgtaggttt ggtttaaata tgatgaagtg gacggaagga aggaggaaga
7681 caaggaagga taaggttgca ggccctgtgc aaggtaagaa gatggaaatt tgatagaggt
7741 acgctactat acttatacta tacgctaagg gaatgcttgt atttataccc tataccccct
7801 aataacccct tatcaattta agaaataatc cgcataagcc cccgcttaaa aattggtatc
7861 agagccatga ataggtctat gaccaaaact caagaggata aaacctcacc aaaatacgaa
7921 agagttctta actctaaaga taaaagatct ttcaagatca aaactagttc cctcacaccg
7981 gtgacgggga tcgcatgcga tatctcgaga tctagcttgg cgtaatcatg gtcatagctg
8041 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
8101 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
8161 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
8221 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
8281 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
8341 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
8401 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
8461 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
8521 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
8581 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
8641 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
8701 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
8761 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
8821 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
8881 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
8941 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
9001 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
9061 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
9121 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
9181 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
9241 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
9301 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
9361 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
9421 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
9481 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
9541 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
9601 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
9661 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
9721 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
9781 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
9841 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
9901 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
9961 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
10021 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
10081 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
10141 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgccagtgc caagctaatt
10201 c
//
