| 质粒名称: | pAAVLINKv1-pCALM1-Cre-CHD7-C-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001733 |
| 产品用途: | 3' AAVLINK plasmid for CHD7 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CHD7 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-CHD7-C-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-CHD7-C-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-CHD7-C-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-CHD7-C-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-CHD7-C-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-CHD7-C-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-CHD7-C-FLAG 7062 bp DNA circular SYN 21-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7062)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7062
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3766
/codon_start=1
/label=CHD7
/translation="VPKRMKRKSWRMTISRKSLPSPKQELSLEGRILMKKAMLP*ALLE
MKPEMDSTWRTEILQ*LSSFMKEHLPSRFGLRIE***TA*TTSVKQC*KANGQ*IGARC
LISKASSQVTHPPQWTAPCRRGALLSSPWSAKPALVGVRTSLRLLSCQRKMPSTSLSLA
SGGGGGEKSKLRPKELPRGEISWRWLPSFESLRWSQKMDKKKL*IYQRPQERQQALPQI
FHLFLQSLSCLMSQHQCLMPLRLKWNCSKQAFRAHPQGISLMAP*WMESLP*RGGGEGG
KMWRDLICFS*ATNGRH*VQRMLR*PKLLKKI*RPHQQETFLLPDSWTQTHGSLLSILK
MGLGWWGKMLLKIRI*LNG*SCTLLTLLICQVMYQRMQMCCFPHFRNRNRNDIDVETLI
NWI*TL*QEKKGCLLSINEMGRRWVELWRLQ*RIYPGGWKKILNLQLLQTGLI*LSSLV
LFLSRCLTAFSLGL*CGERERAEEEEGPKVRSPEQPRPPLLWPPRQGSTLCW*TACLLE
WT*RAFRISRISSRSSWQASWASLQDWQQLPPPEAMRRTLLLCCP*CCQEWRACPTCLA
WAGC*ITLCQLLLETPLLLLVKENRKTALQKERRKEMRMKTRTKTLRKAQMLFRLLTLR
MDLLVLLLPRLDCPQTR*PSTLSSCPQWPRASSTHPCFYLQDWGD*RCLGSQHWQDFRM
PWAPAKKRLLTRLREDPLKMERPLKAAMPRRAWIRLQSPPS*KTK*HRVKS*THLMGGM
K*KTMKMM"
misc_feature 1976..1981
/label=SDS
misc_feature 1979..1984
/label=SDS
misc_feature 1982..1987
/label=SDS
misc_feature 2306..2311
/label=SDS
misc_feature 2318..2323
/label=SDS
misc_feature 3454..3459
/label=SDS
CDS 3770..3835
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3851..4282
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3995..4000
/label=SDS
repeat_region 4325..4465
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4325..4454
/label=AAV2 ITR
rep_origin 4540..4995
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5277..5381
/gene="bla"
/label=AmpR promoter
CDS 5382..6242
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6413..7001
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggttccgaag aggatgaaga
1501 ggaaaagctg gaggatgacg ataagtcgga agagtcttcc cagcccgaag caggagctgt
1561 ctctagaggg aagaattttg atgaagaaag caatgcttcc atgagcactg ctagagatga
1621 aacccgagat ggattctaca tggaggacgg agatccttca gtagctcagc tccttcatga
1681 aagaacattt gccttctcgt tttggcctaa ggatagagta atgataaacc gcttagacaa
1741 catctgtgaa gcagtgttga aaggcaaatg gccagtaaat aggcgccaga tgtttgattt
1801 ccaaggcctc atcccaggtt acacacccac cacagtggac agccccttgc agaagaggag
1861 ctttgctgag ctctccatgg tcggccaagc cagcattagt gggagtgagg acatcactac
1921 gtctcctcag ttgtcaaagg aagatgccct caacctctct gtccctcgcc agcggaggag
1981 gaggaggaga aaaatcgaaa ttgaggccga aagagctgcc aagaggcgaa atctcatgga
2041 gatggttgcc cagcttcgag agtctcaggt ggtctcagaa aatggacaag aaaaagttgt
2101 agatttatca aaggcctcaa gagaggcaac aagctctacc tcaaattttt catctctttc
2161 ttcaaagttt atcttgccta atgtctcaac accagtgtct gatgccttta agactcaaat
2221 ggaactgctc caagcaggcc tttcgcgcac acccacaagg catctcctta atggctccct
2281 agtggatgga gagcctccca tgaagaggag gcggggaagg aggaaaaatg tggagggact
2341 tgatctgctt ttcatgagcc acaaacggac gtcattgagt gcagaggatg ctgaggtgac
2401 caaagctttt gaagaagata tagagacccc accaacaaga aacattcctt ctcccggaca
2461 gctggaccca gacacacgga tccctgttat caatcttgaa gatgggacta ggctggtggg
2521 ggaagatgct cctaaaaata aggatttagt tgaatggctg aagctgcacc ctacttacac
2581 tgttgatatg ccaagttatg taccaaagaa tgcagatgtg ctgttttcct catttcagaa
2641 accgaaacag aaacgacata gatgtcgaaa ccctaataaa ttggatataa acactttgac
2701 aggagaagaa agggtgcctg ttgtcaataa acgaaatggg aagaagatgg gtggagctat
2761 ggcgcctcca atgaaggatc tacccaggtg gctggaagaa aatcctgaat ttgcagttgc
2821 tccagactgg actgatatag ttaagcagtc tggttttgtt cctgagtcga tgtttgaccg
2881 ccttctcact gggcctgtag tgcggggaga gggagcgagc agaagaggaa gaaggcccaa
2941 aagtgagatc gccagagcag ccgcggccgc cgctgctgtg gcctccacgt cagggatcaa
3001 ccctttgctg gtgaacagcc tgtttgctgg aatggacctg acgagccttc agaatctcca
3061 gaatctccag tcgctccagc tggcaggcct catgggcttc cctccaggac tggcaacagc
3121 tgccaccgcc ggaggcgatg cgaagaaccc tgctgctgtg ctgcccctga tgctgccagg
3181 aatggcgggc ctgcccaacg tgtttggctt gggcgggctg ttgaataacc ctctgtcagc
3241 tgctactgga aacaccacta ctgcttctag tcaaggagaa ccggaagaca gcacttcaaa
3301 aggagaggag aaaggaaatg agaatgaaga cgagaacaaa gactctgaga aaagcacaga
3361 tgctgtttcg gctgctgact ctgcgaatgg atctgttggt gctgctactg ccccggctgg
3421 attgccctca aacccgctag ccttcaaccc tttcctcctg tccacaatgg ccccgggcct
3481 cttctaccca tccatgtttc tacctccagg actgggggga ttgacgctgc ctgggttccc
3541 agcattggca ggacttcaga atgccgtggg ctccagcgaa gaaaaggctg ctgacaaggc
3601 tgagggagga ccctttaaag atggagagac ccttgaaggc agcgatgccg aggagagcct
3661 ggataagact gcagagtcct ccctcttaga agacgaaata gcacagggtg aagagctaga
3721 ctcacttgat gggggggatg aaatagaaaa caatgaaaat gatgaaaagg actacaaaga
3781 ccatgacggt gattataaag atcatgacat cgactacaag gatgacgatg acaagtaaga
3841 attctgatga ctcgagataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
3901 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
3961 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttagtt
4021 cttgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
4081 ttgggcactg acaattccgt ggtgtttatt tgtgaaattt gtgatgctat tgctttattt
4141 gtaaccatct agctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
4201 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
4261 ggagatgtgg gaggtttttt aaagatctgg agaccggtgt ggcacgtgcg gaccgagcgg
4321 ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact
4381 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc
4441 gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg
4501 tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat
4561 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag
4621 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
4681 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
4741 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
4801 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
4861 caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
4921 tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
4981 taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
5041 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
5101 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
5161 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
5221 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
5281 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
5341 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
5401 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
5461 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
5521 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
5581 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
5641 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
5701 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
5761 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
5821 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
5881 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
5941 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
6001 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
6061 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
6121 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
6181 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
6241 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
6301 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
6361 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
6421 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
6481 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
6541 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
6601 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
6661 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
6721 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
6781 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
6841 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
6901 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
6961 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
7021 ttttacggtt cctggccttt tgctggcctt ttgctcacat gt
//
