| 质粒名称: | pQL62 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002504 |
| 产品用途: | Synthetic mammalian ETS TF-dependent SEAP expression vector ((GGAA)3-PhCMVmin-SEAP-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: |
Synthetic
|
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | SEAP |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
pQL62
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pQL62载体质粒基本信息
pQL62质粒图谱载体图谱和pQL62载体序列质粒序列多克隆位点信息
pQL62质粒载体简介
pQL62质粒序列载体序列
LOCUS pQL62 6426 bp DNA circular SYN 04-JAN-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM recombinant plasmid
REFERENCE 1 (bases 1 to 6426)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 2, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6426
/mol_type="other DNA"
/organism="recombinant plasmid"
primer_bind 213..253
/label=QL121
primer_bind complement(234..274)
/label=QL122
misc_feature 234..253
/label=GGAA
primer_bind 254..276
/label=Oscl23-1
misc_feature 260..407
/label=hcmvmin
misc_feature 329..343
/label=New Feature
primer_bind complement(392..407)
/label=oYW11
misc_feature 430..453
/label=New Feature
primer_bind 454..480
/label=Oscl13-1
misc_feature 455..1975
/label=SEAP
primer_bind complement(1955..1983)
/label=Oscl23-2
primer_bind complement(1957..1975)
/label=Oscl13-2
terminator 2023..2250
/label=bGH PA
misc_feature 2918..2995
/label=SV40 ORI
CDS 3137..3928
/label=Kan/neoR
primer_bind complement(4268..4288)
/label=M13-rev
misc_binding complement(4294..4316)
/label=LacO
promoter complement(4321..4350)
/label=lac
CDS complement(5433..6092)
/label=AmpR
promoter complement(6332..6360)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgtacgga
241 aggaaggaag atcgctagct cgagctcggt acccgggtcg agtaggcgtg tacggtggga
301 ggcctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg
361 ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc ccgaattcga
421 gctcgcccgg ggatcctcta gagtcagctt ctgcatgctg ctgctgctgc tgctgctggg
481 cctgaggcta cagctctccc tgggcatcat cccagttgag gaggagaacc cggacttctg
541 gaaccgcgag gcagccgagg ccctgggtgc cgccaagaag ctgcagcctg cacagacagc
601 cgccaagaac ctcatcatct tcctgggcga tgggatgggg gtgtctacgg tgacagctgc
661 caggatccta aaagggcaga agaaggacaa actggggcct gagatacccc tggccatgga
721 ccgcttccca tatgtggctc tgtccaagac atacaatgta gacaaacatg tgccagacag
781 tggagccaca gccacggcct acctgtgcgg ggtcaagggc aacttccaga ccattggctt
841 gagtgcagcc gcccgcttta accagtgcaa cacgacacgc ggcaacgagg tcatctccgt
901 gatgaatcgg gccaagaaag cagggaagtc agtgggagtg gtaaccacca cacgagtgca
961 gcacgcctcg ccagccggca cctacgccca cacggtgaac cgcaactggt actcggacgc
1021 cgacgtgcct gcctcggccc gccaggaggg gtgccaggac atcgctacgc agctcatctc
1081 caacatggac attgacgtga tcctaggtgg aggccgaaag tacatgtttc ccatgggaac
1141 cccagaccct gagtacccag atgactacag ccaaggtggg accaggctgg acgggaagaa
1201 tctggtgcag gaatggctgg cgaagcgcca gggtgcccgg tatgtgtgga accgcactga
1261 gctcatgcag gcttccctgg acccgtctgt gacccatctc atgggtctct ttgagcctgg
1321 agacatgaaa tacgagatcc accgagactc cacactggac ccctccctga tggagatgac
1381 agaggctgcc ctgcgcctgc tgagcaggaa cccccgcggc ttcttcctct tcgtggaggg
1441 tggtcgcatc gaccatggtc atcatgaaag cagggcttac cgggcactga ctgagacgat
1501 catgttcgac gacgccattg agagggcggg ccagctcacc agcgaggagg acacgctgag
1561 cctcgtcact gccgaccact cccacgtctt ctccttcgga ggctaccccc tgcgagggag
1621 ctccatcttc gggctggccc ctggcaaggc ccgggacagg aaggcctaca cggtcctcct
1681 atacggaaac ggtccaggct atgtgctcaa ggacggcgcc cggccggatg ttaccgagag
1741 cgagagcggg agccccgagt atcggcagca gtcagcagtg cccctggacg aagagaccca
1801 cgcaggcgag gacgtggcgg tgttcgcgcg cggcccgcag gcgcacctgg ttcacggcgt
1861 gcaggagcag accttcatag cgcacgtcat ggccttcgcc gcctgcctgg agccctacac
1921 cgcctgcgac ctggcgcccc ccgccggcac caccgacgcc gcgcacccgg gttaagcggc
1981 cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
2041 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
2101 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
2161 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
2221 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
2281 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
2341 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
2401 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
2461 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
2521 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
2581 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
2641 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
2701 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
2761 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
2821 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
2881 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
2941 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
3001 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
3061 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
3121 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
3181 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
3241 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
3301 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
3361 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
3421 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
3481 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
3541 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
3601 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
3661 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
3721 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
3781 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
3841 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
3901 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
3961 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
4021 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
4081 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
4141 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
4201 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
4261 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
4321 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
4381 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
4441 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
4501 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
4561 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
4621 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
4681 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
4741 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
4801 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
4861 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
4921 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
4981 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
5041 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
5101 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
5161 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt
5221 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
5281 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
5341 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
5401 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
5461 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
5521 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
5581 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
5641 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
5701 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
5761 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
5821 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
5881 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
5941 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
6001 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
6061 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
6121 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
6181 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
6241 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
6301 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
6361 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
6421 gacgtc
//
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